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Complete genome sequence of a variant of maize-associated totivirus from Ecuador

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Abstract

The complete genomic sequence of a variant of the recently reported maize-associated totivirus (MATV) from China was obtained from commercial maize in Ecuador. The genome of MATV-Ec (Ecuador) (4,998 bp) is considerably longer than that of MATV-Ch (China) (3,956 bp), the main difference due to a ≈ 1-kb-long capsid-protein-encoding fragment that is completely absent from the Chinese genome. Sequence alignments between MATV-Ec and MATV-Ch showed an overall identity of 82% at the nucleotide level, whereas at the amino acid level, the viruses exhibited 95% and 94% identity for the putative capsid protein and the RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis of the viral RdRp domain indicated that MATV-Ec and MATV-Ch share a common ancestor with other plant-associated totiviruses, with Panax notoginseng virus A as the closest relative. MATV-Ec was detected in 46% (n = 80) of maize plants tested in this study, but not in endophytic fungi isolated from plants positive for the virus.

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Acknowledgements

The authors would like to express their gratitude to maize growers for granting access to their fields and to researchers at INIAP-Portoviejo, especially to Alma Mendoza who helped with sample collection.

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Authors and Affiliations

  1. Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador, CIBE, Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador

    Robert A. Alvarez-Quinto, Rodrigo F. Espinoza-Lozano & Diego F. Quito-Avila

  2. Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida, Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador

    Carlos A. Mora-Pinargote & Diego F. Quito-Avila

Authors
  1. Robert A. Alvarez-Quinto
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  2. Rodrigo F. Espinoza-Lozano
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  3. Carlos A. Mora-Pinargote
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  4. Diego F. Quito-Avila
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Corresponding author

Correspondence to Diego F. Quito-Avila.

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Funding

This work was partially funded by the Ecuadorean Science and Technology Secretariat (SENESCYT), through the National Emblematic Program PROMETEO.

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All the authors declare they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Alvarez-Quinto, R.A., Espinoza-Lozano, R.F., Mora-Pinargote, C.A. et al. Complete genome sequence of a variant of maize-associated totivirus from Ecuador. Arch Virol 162, 1083–1087 (2017). https://doi.org/10.1007/s00705-016-3159-2

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  • DOI: https://doi.org/10.1007/s00705-016-3159-2

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