Abstract
Ancient bones, presumably dating back to 6000 BC and 1500 BC, have been characterized employing different physical analyses (optical and electron microscopy, characteristic X-ray fluorescence, and others) and prepared to be submitted to further investigations by nuclear techniques for dating analysis. Particular attention has been devoted to the freeze-drying process (also known as lyophilization) of the biological samples, which is based on the tissues drying under frozen environment, enabling removal of water and absorbed gases. Such a process presents different advantages, such as the possibility to store the samples at room temperature preserving their chemical properties, and the enhancement of the weight concentration of elements in the matrix. In this work attention is devoted to freeze-drying of cortical and trabecular bones, which loss water slowly with respect to soft tissues. The essential parameters to control during the process are the temperature and the pressure, the maintenance of a low gas pressure and the drying time, allowing the proper ice sublimation and the water degassing from the sample. The aim of the presented work is that to describe the lyophilization of cortical bones, to freeze and subsequently store the sample at low pressure, sublimating the ice on their surface and eliminating the gas under vacuum conditions. Experimental measures were performed to reduce the water content in the range of 1–5 wt.%. Measurements of water desorption, electronic microscopy before and after their treatment process, as well as Raman and FTIR spectroscopy were performed on the treated samples. The described procedure allows to prepare samples at high carbon concentration from which, employing nuclear analysis, it is possible to evaluate the content of stable isotopes and of radiocarbon, which have a crucial relevance for dating of biological samples.
Similar content being viewed by others
References
S. Talamo, H. Fewlass, R. Maria, H. Jaouen, Sci. Technol. Archaeol. 7, 62 (2021). https://doi.org/10.1080/20548923.2021.1944479
A. Merivaara, J. Zini, E. Koivunotko, S. Valkonen, O. Korhonen, F.M. Fernandes, M. Yliperttula, J. Control. Release 336, 480 (2021). https://doi.org/10.1016/j.jconrel.2021.06.042
S.M. Ott, Am. J. Nephrol. 47, 373 (2018). https://doi.org/10.1159/000489672
A.A. Ariffin, H.H. Chan, N. Yusof, S. Mohd, S. Ramalingam, W.M. Ng, A. Mansor, JUMMEC 22, 66 (2019). https://doi.org/10.22452/jummec.vol22no1.10
H. W. Xiang, Chapter 6 - Vapor Pressures, in The Corresponding-States Principle and its Practice, (Elsevier, 2005). https://doi.org/10.1016/B978-044452062-3/50006-3
E.F. Morgan, G.U. Unnikrisnan, A.I. Hussein, Annu. Rev. Biomed. Eng. 20, 119 (2018). https://doi.org/10.1146/annurev-bioeng-062117-121139
W. Gong, S. Yang, L. Zheng, H. Xiao, J. Zheng, B. Wu, Z. Zhou, J. Cult. Herit. 35, 116 (2019). https://doi.org/10.1016/j.culher.2018.06.002
G. Quarta, M. D’Elia, E. Ingravallo, I. Tiberi, L. Calcagnile, Radiocarbon 47, 207 (2016). https://doi.org/10.1017/S0033822200019706
G. Aprile, L. Calcagnile, J. De Grossi Mazzorin, C. Minniti, R. Montefinese, G. Quarta, I. Tiberi, Concerning the extinction of the wild horse in Italy and the newly introduction as domesticate: recent evidence from Grotta dei Cervi - Porto Badisco (Otranto, south Italy), 3rd IMEKO International Conference on Metrology for Archaeology and Cultural Heritage, 2017, Lecce, Italy. https://www.imeko.org/publications/tc4-Archaeo-2017/IMEKO-TC4-ARCHAEO-2017-003.pdf
R. Longin, Nature 230, 241 (1971). https://doi.org/10.1038/230241a0
S. Suvarnapathaki, X. Wu, T. Zhang, M.A. Nguyen, A.A. Goulopoulos, B. Wu, G. Camci-Una, Bioact. Mater. 13, 64 (2022). https://doi.org/10.1016/j.bioactmat.2021.11.002
Z. Shahbazarab, A. Teimouri, A.N. Chermahini, M. Azadi, Int. J. Biol. 108, 1017 (2018). https://doi.org/10.1016/j.ijbiomac.2017.11.017
A. Trujillo-Mederos, I. Alemána, M. Botella, P. Bosch, J. Archaeol. Sci. 39, 1072 (2012). https://doi.org/10.1016/j.jas.2011.12.005
Fytik software - https://fityk.nieto.pl/
I. Aguilar-Hernández, D.L. Cárdenas-Chavez, T. López-Luke, A. García-García, M. Herrera- Domínguez, E. Pisano, N. Ornelas-Soto, Biomed. Opt. Express 11, 388 (2020). https://doi.org/10.1364/BOE.11.000388
X.N. He, J. Allen, P.N. Black, T. Baldacchini, X. Huang, H. Huang, L. Jiang, Y.F. Ludoi, Biomed. Opt. Express 3, 2896 (2012). https://doi.org/10.1364/BOE.3.002896
C.K. Rojas-Mayorga, A. Bonilla-Petriciolet, J. Silvestre-Albero, I.A. Aguayo-Villarreal, D.I. Mendoza-Castillo, Appl. Surf. Sci. 355, 748 (2015). https://doi.org/10.1016/j.apsusc.2015.07.163
H. Gheisari, E. Karamian, M. Abdellahi, Ceram. 41, 5967 (2015). https://doi.org/10.1016/j.ceramint.2015.01.033
L. Torrisi, Pol. J. Med. Phys. Eng. 25, 193 (2019). https://doi.org/10.2478/pjmpe-2019-0026
L. Torrisi, A. Italiano, A. Torrisi, Appl. Surf. Sci. 387, 529 (2016). https://doi.org/10.1016/j.apsusc.2016.06.153
A. Georgopoulou, F. Papadogiannis, A. Batsali, J. Marakis, K. Alpantaki, A.G. Eliopoulos, C. Pontikoglou, M. Chatzinikolaidou, J. Mater. Sci. Mater. Med. 29, 59 (2018). https://doi.org/10.1007/s10856-018-6064-2
E.T. Moghadam, M. Yazdanian, M. Alam, H. Tebyanian, A. Tafazoli, E. Tahmasebi, R. Ranjbar, A. Yazdanian, A. Seifalian, J. Mater. Res. Technol. 13, 2078 (2021). https://doi.org/10.1016/j.jmrt.2021.05.089
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Torrisi, A., Proverbio, E. & Serra, A. Ancient Bones Characterization and Preparation Through Freeze-Drying Process. Int J Thermophys 43, 126 (2022). https://doi.org/10.1007/s10765-022-03054-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10765-022-03054-5