WO2015092549A2 - Biologicals for plants - Google Patents

Biologicals for plants Download PDF

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Publication number
WO2015092549A2
WO2015092549A2 PCT/IB2014/003190 IB2014003190W WO2015092549A2 WO 2015092549 A2 WO2015092549 A2 WO 2015092549A2 IB 2014003190 W IB2014003190 W IB 2014003190W WO 2015092549 A2 WO2015092549 A2 WO 2015092549A2
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WO
WIPO (PCT)
Prior art keywords
plant
strain
increase
bacillus
composition
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PCT/IB2014/003190
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French (fr)
Other versions
WO2015092549A3 (en
Inventor
Mari Ellen Davis
Elizabeth GALBRAITH
Shelly Gebert
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Dupont Nutrition Biosciences Aps
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Publication of WO2015092549A2 publication Critical patent/WO2015092549A2/en
Publication of WO2015092549A3 publication Critical patent/WO2015092549A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Definitions

  • Bacterial strains, compositions, and method'; for redaciag overall damage a d l sses in plasu perionruusce, health, vigor and yield are disclosed.
  • bacterial sunlas, compositions, and ioe.hwl:-: for treating seeds, soil. plants, or pin: : ⁇ ao to reduce bsetcriaf i ⁇ mgai. and/or nematode damage are disclosed, in one embo iment, bacterial strains. corapositioas. ; and meth ds lor treating seeds, soil plants, o plant parts to increase plant performance arc disclosed.
  • Plants are often the targets of attack from pathogens, sach as acteria, fang:, and nematodes. Slow, but steady, progress has beers made in the last few decades towards developing more efficient methods rtd compositions for controlling pathogen infestations in plants.
  • Chemical pesticides have traditionally been nsed to control pathogen mfestadons. However, : ⁇ . ⁇ . ⁇ ; ⁇ are se eral disadvinaages to nsing chemical pestleid3 ⁇ 4l agents, such as lack of seleebvity, persistence in the envnwraneni and toxicity.
  • the dlsckwnre relates to « composition comprising one or more bacteria! strains selected from the group eopsisimg of B clHus mykiiquif cie .
  • Baci!l iiehe forms, BaciUux suhkiis, and a nibaciil poiy iyxa.
  • the disclosure relates to a composition eompnsmg one or snore bacterial strains selected ⁇ : the group consisting of tea new ?nyk ⁇ !i utf ⁇ * kii'Hs- ⁇ 2 ' 7 (NRRL 8-501 Sg Baciiius o y! l?gmf c fis BS2G84 (NRRL B-50013i, Beciihjs my iiq ifackns 15AP4 (ATCC PTA-6>07), f,a aw.
  • amy nqt fhckm 3AP4 (ATCC PTAfoSObg B isius mykBquifsckm 1 vs.yoi (NRRL - 50-104). Bacillus o ylol q tck ⁇ 278 (NRRL B-50034), Bacillus amyBBlquBack 1 13 (NRRL B-S0500).
  • die islcosrue relates to a conge tio , comprising at ast two bacterial strains selected Rom the gtotsp consisting ⁇ > ⁇ Bacillus amyloBqkfhcle .
  • the strains can of the same species or different species.
  • the composition further comprises a hioooniroi agent selected from l « group ⁇ ⁇ - ⁇ . ong of bacteria, Rmgi, yeast proRrzoas, viruses, eotomopsihogefdc n-ooao-Oi- botanical extracts, proteins, secondary metabolites, and mrtocitlants.
  • a hioooniroi agent selected from l « group ⁇ ⁇ - ⁇ . ong of bacteria, Rmgi, yeast proRrzoas, viruses, eotomopsihogefdc n-ooao-Oi- botanical extracts, proteins, secondary metabolites, and mrtocitlants.
  • die oomposinon comprises at least two strains selected 0 an the group consisting of: Bacillus amykaiqui/ackas BS2 " l (NRRL B-S015), Be, .7.0.0 amyi /igui fastens BS20S4 (NRRL BA00I3) Bacillus myloligw/hc ss 15AP4 (ATCC PTA-OaO/f. Baalim
  • amylaliquifiadens 3AP4 (AfCC FTA-0505), Bac-ca.- a y lfaek Id o.vd ( RRL B-50504), .Bacillus a y llqidfaaem ABP278 (MRRL B-gSOgBaf Bacillm amykBlq tfhc m 1013 (NRRL B- 50509), Baviiius 918 (NRRL B-50508), Bticiliw amy Bqdfackas 22CP1 (ATCC PI A-650S), Bacillus amykBqiqfac is BSI8 (NRRI B- 50033), Bacillus iichenlfartvis BA842 O R L B- 50516), Bacillus h eniforntis BL21 (NRRL ⁇ -5 ⁇ 4), Bacilli sabBBs 3 P5 (NRRL B--50R10), and B;
  • the composition c m rises Bacillm .aacB- ga yd-. ⁇ ⁇ . ⁇ 22CP1 and Bacillus amylaik i/ac x I 5AP4. !rt still another e b druent the composition comprises Bacillm ainykliquifitckas 22CF i rid i -/do amyloHguBackas BS ! 8, in yet arsother enRsa-drmeot, the composites* comprises Bacillm my tq&ifyck-m BSI8 and B ctHus amylollqiBfack 15AP4.
  • the composition ioono comprises one or more agroehenneai!y a t ve compounds selected IVom die group consisting of: an insecOcide, a fungicide, a bactericide, and a nemaiicide.
  • its? composition further comprises a compound selected from the group consisting of a saRmer, a ⁇ -chevooiigosaecharide, an Isofiavone, and a ryanodine receptor modulator.
  • the composts n comprises one or more agfcshem ically active eornpoands, wherein one compound Is ryoaxypyr.
  • the conges ion comprises one or more agroohemicaliy active compounds, wherein one c- ope and is cya wpyr,
  • the composition further comprises at least one seed, plant, or plant part, in one embodiment, the seed, plant or plant part is genetically modified .
  • the compostion inhibits the crowd ' ! of one or m e plant athogens including bm n t limited to & bacteria, a fungus, a - irus : . a protoaea, or a nematode.
  • the eornposbon is in an effective amount to improve plant performance including but ftot hmi ed to mcreased root, formation, increased root mass, increased root function, increased shoot height, increased shoot function, increased flower bud presence, increased flower bod formation, increased seed germination, increased total plant wet weight, and increased total plant dr.. w eight.
  • the disclosure relates to a med ' sod comprising spplymg a composition comprising one or more bacterial strains selected from the group consisting ⁇ ⁇
  • the disclosure relates to a method comprising applying a composition comprising one or more bacterial strains seieeled from the group consisting oi .0. ;. who iyioikpjifiH.' 'i- . .f a o 7 w chemfbrf : .Pa .ffw . -J .O/W -.no/ ' v.vvwa /a,. -: ; ⁇ .,.>. ⁇ > . > .g-. ⁇ ; ⁇ . ⁇ to a seed, a plant, plant pari or sod Irs an effective amount to achieve ar?
  • erlbct selected iforn the goaup consisting of inhibit a plant pathogen, improve plant performance, improve plant yield, improve plant vigor, increase phosphate avaliabiiity, increase production of a plant hormone, uictease root fo mtknv increase shoot heigh- in a piano increase leaf length of a plant, increase flower bud formation of a plant, increase total plant fresh weight, increase total plant dry weight, and increase seed ermi tion.
  • the disclosure relates to a method comprising applying a composition contprisiog at least two bacterial mams selected from the group consisting of Saei!iiiH
  • the strains can be of the same species o s ⁇ different species-
  • the method com rse applying a composition further comprBmg a biocontrof agent, wherein the bioeoniroi agent selected f : a de g oup c nsistin of bacteria, hmgi yeast, protoi-oas, viruses, srmsmopathogestic nematodes, botanical extracts, proteins, secondary metabolites, and inaoculents.
  • the method ⁇ ⁇ >: ⁇ : ⁇ applying a com osiio bather comprising at least ns-o strains are selected irmn the rou consistin of: Baillus ⁇ loU talacie BS2? (NRRL ⁇ 50 5). Bacillus amyl uifackns BS2084 (NRRL B-5QGD), Bacillus myloliqtiifac m 15AP4 ATCC PTA-650? ⁇ , &adh ⁇ s a l liqulfaciem 3APd (ATCC PTA-6 6), Bacillus ur-rylonq if cie LSSAOI (NiiRL b b 101;.
  • gacilim myloh ' quia iens ASP278 (NRRL B- 50b34 ⁇ Bacith amyteliq iftteterts 1013 (NRRL B-50509), Bacillus a yhliqukaaens 91$ fN ' RRL B-5G508), Bacill amyloH.]uif ciew 22CP1 (ATCC PTA-65G8), Bacitl iwiollquifyck BSI8 (NR 3 B-50633) ⁇ Bacillus iicheniformis BAS 2 (NRPi. C-Ac3dny R.- t ;H U . ⁇ .
  • the method comprises applying a composition eomprsmg .BoRRs cmn'hHquiJa ris 22CP! and Bacillus ⁇ ldl uifyciens 15AP4,
  • the method comprises applying a composition comprising Bacillus a ⁇ loiiqmfadc 22CP!a;3 ⁇ 4! 3 ⁇ 4ea%s a/ttyloiiqtttfiKtem BS1S.
  • the method comprises applying a composition comprising 3 ⁇ 4ici3 ⁇ 4 « ; my liqxifixc& BShS and B l ' hs amy llquifhc m ISA ..
  • the method comprises applying a composition: comprising one or more agr chemicsUy active compounds selected Bom the group consisting of: an insecticide, a fungicide, a bactericide, a d a nem Suitede,
  • the method comprises applying a composition Birdie r comprising a compound selected R m she group consisting of a saiener, a !ipo-ohitoollgosaccharlde, isoflavone, and a ryanodine receptor modulator.
  • the method comprises applying corn-position comprising one or more agKfcbemical!y active ciits otwsds, wherein one compound is synaxypyr, in another embodiment, the method comprises applying a composition comprising one or more agrochemRaSly active compoun s, wherein one compound s cya3 ⁇ 4:ypyr, In another emb diment, the method comprises applying a composition com risin rynaxypyr and eyaxypyr.
  • the method comprises applying the composition in an effective amount R ; inhibit growth of a plant pathogen, including but not limited to bacteria, a fungus, a nematode, a virus, a protozoa.
  • a plant pathogen including but not limited to bacteria, a fungus, a nematode, a virus, a protozoa.
  • c mprises apple ing e composition in an effocii ve amoom m bo ove plam psi Bce n hsd ⁇ bar not Imihe-d to increased root formation, increased root mass, increased root bincbon, increased shoot height, increased seed germination, increased shoot batchon. ifH-mased Bower bud presence, increased flower bnd formation, incnaased total plant wet weight, and soereased total pimt dry weight.
  • t e ⁇ h - k-.nr rel tes to an isolated Aoe/bow shram for improv ing plant performance, plant vigor, or plant yield, wherein the Bectihit stain comprises the BdhA en*, and produces :od- AA> accde , ⁇
  • Irs one embodiment, ; ⁇ disclosure relates to an isolated Bacillus strain for improving plant performance, plant vigor, or plant yield, wherein th « Bacittm strain comprises de trp B gene, md produces lndole-3-aeetle acid.
  • the disclosure relates to an isolated Jfc_%3 ⁇ 4* strain lor improving plant performance, lant vigor, or lant yse!d wherein the Sa iH strain comprises the phoP gene, and has the ability m solnhiliA? phosphate.
  • the disclosure relates to an isolated .bbmb/m strain for improving plant performance, plant vigor, or plant yield wherein the JntcdAct stride comprises the phoio BdhA, 3 ⁇ 4nd t pAB genes.
  • the disclosure relates to an isolated Sue nowadays fas sirarn for improving, plant performance, plant vigor, or plant yield wherein the Ecsciikfs strain produces indoiew -acetic acid and has the ability to sobrblii e phosphate.
  • FiCA 1 is a photograph of ins pmoP PGR product (134 base pairs (bp)) on a 1% agarose gel Samples were loaded in t e following order f om kit to right: 100 bp nolec &r rnler, bAebb.v
  • FIG, 2 is a photograph ofthe AbA PAR prodtmt (336 bp) on a ]% agarose gel, Samples were loaded In the foilowmg onier from left to right: 1 0 bp molemnar ruler, I A2B42 (positi e toniroil A 0157 (negative consol), LSSA01, 220PP i 5A-P4, 3A P , BS2 v BS; A BtAOIPb ABP27A BAB42. BL2? S ABP!dd, and 100 bp molecular rtder.
  • FIG. 2 is a graph depicting the standard curve and trend line ofbAA standard dilutions: 0, 74*124, 15.625, 31,25, 62,5, 125, 250, and 500 opnr
  • FIG. 4 s a hotograph of th tigoAB PGR product (2,069 bp) or* a ! % agarose gel .
  • Sampl s were loaded in the followin order from left to right; i 00 bp molecular ruler, F2B42 (positive control), E. coli O! S? (ne ative comroi), LSSA0 R 22CPL 15A-P4, 3 ⁇ - ⁇ 4, B3 ⁇ 427, Bb
  • FIG. SAG is a i t re of the right bad flower stage.
  • FIG 5A-2 is a psoow of the ' cracked" bod flower sta s
  • FIG - ' ⁇ ⁇ .. -. ⁇ is a picture of the " ⁇ ! of color flower stage.
  • FIG. SA-4 rs a icture of the folded petals flower stage.
  • FIG. SA G is a picture of the open flower stage
  • FIG. 58 s a picture indicating whore the (lower uds were removed at the natural indentation of bod formation (biacT arrow) tor reproductive dry weigh; measurements.
  • FIG. 6 G a line graph depicting da. average percent genninarioa of corn seed grows; is; the presence of iioorf jov treatment or control.
  • FIG, 7 is a bar graph depicting the average seed hag dry weight of com seed grown in the presence of B cHi treatiuent or control.
  • strains, compositions nd methods are not limited in their application to the detahs of construction and the arrangement of the components set forth in the following description or iihwtrated in the drawings and Figures.
  • the bact ial strains, compositions and methods are capable of other embodiments Of being ps acrieed or carried out in w3 ⁇ 4Hoos ways. Also, it is to be understood that rise phraseology and terminology employed herein Is for the purpose of description and should not be regarded as limiting.
  • the ru rses'lcal ranges in this disclosure are approximate, aod thus, may include values outside of dse rasge e:nles.3 otherwise usdtceled Numerical ranges include ail values from and Including the lower and the upper values, in increments of one unit, rovided that there is a separation of at bast two units between any Sower valu and any higher value.
  • a compositional physical or other pswpcrry such as, for evampie, nubecsdar weight, tesnpefaSrue, ore, .
  • Is Irons 100 to 1 ,000 is is rmended that all Irsdw ideal va lues, such as 100, i O i , l Od wto. , sad sub ranges, such as 100 to 144, 155 to I 70, b o to 200. a. a . are expressly enumerated.
  • c tain n values which are loss than one or containing fractional numbers greater O ca; one (e.g., 1 . 1 , 1.5, o;. ' ⁇ , one unit is considered to be 0.0001 , 0.00 L 0.01 or 0, 1 , as appropriate.
  • Asd red ulster refers to the action of introducing the imin and or composition to a n environme d in need of pathogen inhibition or improved plant performance.
  • agrochemicaiiy active compound refers So any subsn-mee tsOat is r m3 ⁇ 4. be customarily used tor treating plants including but not leaned fungicides, bactericides,, mseef aides, acarseides, uernaiieldes, inol iosekades, safeners, plant growth regulators and plant nutrients as wel l as tnlceoorgaeisin ,
  • a ccsrnpositlon may be a liquid, a heterogeneous mixture, a hosn geneous mixture, a powder, a solution, a d!sperskav lyophilieed, freeee-ds ed, or any combination thereof.
  • a ive amount refers to & c-uantity of bacterial strain or cosnposltion sufficient to inhibit rowt of a pathogenic mk ; m r n i3 ⁇ 4s or to impede the rate of growth.
  • the term ' effective &mo «»r refers to a quantity of bacterial strain or eomposltlon sufficient to improve plant perfommnoe.
  • thai an effective a?3 ⁇ 4o «nt of bacterial strain or composition may not reduce the numbers of pests/paihogeus, but is effective in decreasb)g damage- to plants as a result of a pesi/pathogen. Accordingly, the efficacy of s treatment can bo determ ined via any direct or indirect sndpoims.
  • a pesheidaby effective amount may redsoe isafhogen damage to s eds, roots, shoots, or foliage of lan ; that are reated compared to those that are w e u -
  • %inproviis or iiKressing p ' mi perforomnee" rotors fo improving r increasing the yield of the plant r irapmving the vigor of the plant or improving bods the yield and ie vigor of the plant.
  • the yield is increased by at least from about 0,5% to about 1 %.
  • auodier ernbodarisnr the yield is increased by at leas from about 1 % to aisoai 2%.
  • t o yield is increased by at beast if - en about 2% to about 4% .
  • the yield is increased by at least about from 43 ⁇ 4 t ahead 5%.
  • I anot e embod iment > % yield is increased by at least about from 5% to about 10%
  • the yield is increased from about 1 0% to about 20% or greater than 20%.
  • the phrase " Improving or increasing the vigor" of a plant relates to an increase or a pprovement or the vigor rating, or Use stand fthe number of piano:; ow unh of area), or do; piani height, or the plant canopy, or the visual appearance (such as grows; leaf color ⁇ , or the- root raon or root function, or root formation, or root mass ; oi' emergence, or prole content, or i creased ttllstrng, or bigger leaf blade, or longer leaf length, or wider leaf, or increased flower bud presence or formation, or less dead basa l leaves, or stronger hhers, or less ferrihzer needed, or iess seeds needed, o ⁇ more productive til lers, ss ear lier dowering, or early grain maturity, or less plans verse ( lodging), or irrovawed shoot growrh, c-r increaoed shoot hssg
  • the term "inhibit" 1 refers to destroy, prevent, control, decrease, slow or othemioe Interfere with the growth or s rvi al of a pathogen when compared to the growth or surviv al of the pathogen in an untreated control.
  • to "inhibit" la io desrroy prevent, control. decrease, sl or otherwise interfere with ihe growth r 1 survi al of s a hogen by at least about 3% st km. abo t 100% ; or any value In between for example at least about 10%, 15%, 20%, 25%, 30%, 55%, 40%, 45%, 5 , 55%, 6 55, 6S%, 70%.
  • to "Inhibit" is to destroy, psev-vm. corneal, decrease, slow or otherwise interfer wdh the growth or sun - wd of a pathogen by at least abooi Moid or more, for example, about 1 -5-fold to shout ! 00 ⁇ fold, or any vahne in et een for example by at least about 2.0, 2.5, 1.0, 3.5, 4,0, 4 ⁇ 5 ,0, 5.5, 6.0. 6.5, 7.0, 7% 8.0, 15, 9.0, 9.5, ⁇ ⁇ , O. 20, 25, 30. 35, 40, 45, . 55, 60, 65. 70, 7$, 3 ⁇ 4% eS, 90 ; 95-fo!d when compared % the growth or survival of the pathogen in an mdreated control
  • Inhibition of die gro wth of a pathogen occurs smmediatejy, In another aspect inhibition of the growth of a path gen occurs one minute after, 30 minutes after, 45 minmes after, one h- r a ter, earn hours after, tons hoars after, sis hoars af!er, w ew hoar;? after, eigh een hour* after, or one day or more after a bacterial strain or composition d s losed herein s applied k- a plmi material.
  • inhibition of the grow th of a pathogen lasts lor ⁇ * ⁇ provides protection for greater thtm one or more days, two or more days, three or more days, four or mo e days, five or more days, one week., two weeks, three weeks, one month or snore after a bacterial strain or composition disclosed herein is applied to the subjee> m terial.
  • inhibition of the growth of a pathogen lasts from W K- to severs days, dose sewers to ) 4 days, frorn 14 to 21 days, or from 1 to 30 days or more
  • inhibition of pathogen growth lasts until a plant material is consumed or discarded.
  • liquid means a substance In the fluid state of matter having no fmed shape but a fixed volume. Liquids may be liquid at room temperature and pressors.
  • the term "paihogen” Includes bet is not I med to pathogeme fungi, bacteria, and nematodes, as well as arthropod pests such as insects, arachnids, ehilopods, and dipiopods.
  • pathogen * ' may be present at an point in the lifetime of a product for example, originating from one or more of the ollo i g: the environment from which the product was obtained and/or dw microbiological medio of the product in its raw or unprocessed state (e.g. native to the product) and/or any handling and 'or processing steps m; - s the Ki ecto'eness.aneffoeiw eness of packaging andon- storage conditions of the product.
  • pathogenic ntlcroorgtunsm refers to a 'microorganism that is capable of causing disease in human, an animal or a piarrt.
  • the "'pathogenic microorganism '' * may be present at any point in the lifetime of a product, for example,, originating from one or more of the following: the eovh omnent f o??:) which the product was obtained and/or dee microbiological quality of the prodecs m its >: ⁇ -: ⁇ -.- or nnproeessed stats (e.g.
  • the seam "plant” refers u- ail glacis sad plsm po ulatio s, s ch as dessrabie and undesirable wild plants, oakivars and plant a ieties (whether nor proteciable by plant varlegv or l in breeder's riaius).
  • Cu tlvars and plant varie ies can be piants obtained by conventional propagation and breeding oterhods that can be assisted or sopplemeoied by one or more biotechnologieal methods such as by use of doable baploids, protoplast fusion, random ami directed o-miageoesis. molecular or genetic markers or by bioeoglsneer g and genetic engineering methods.
  • plant parts refers to ah above ground and betosv ground parrs and organs or plants sacs as shoot, leaf, blossom and «x;g whereby o example le ves, needles, stems, branches. btossw'ns, banting bodies, irons and seed as wed as rooty aider:- coons and ddaomes are nschsded. Crops and vegetative and generativ e propagating material, for example endings, eotms, rhie'omes, ushers, runners and seeds also belong to plant parts.
  • the terra "plant heakh” is to be nadcrstood to enoie 3 ⁇ 4 condition of the plant and/or hs products which is deterrnmed by several Indicators alone or in combination wit each other snch as yield (e.g. increased biomass ami/or increased content of valuable ingredients), plant vigor (e. . disproved plant growth and/or greener leaves ("greening effect” ⁇ ), qualify (e.y. improved content or composition of certain Ingredients) and tolerance to able-tic and/or biotic stress.
  • yield e.g. increased biomass ami/or increased content of valuable ingredients
  • plant vigor e. . disproved plant growth and/or greener leaves ("greening effect” ⁇
  • qualify e.y. improved content or composition of certain Ingredients
  • tolerance to able-tic and/or biotic stress e.y. improved content or composition of certain Ingredients
  • 'prev ntin refers to the pathogen contamination of a product to which the strain or composition is applied and has an evtended shab b y- and/or increased time frame before a specified amoum of eootaimnant is present.
  • slaiil- li!e and/or time frame Is extended mai o ⁇ n3 ⁇ 4creased when eoatpared to a control product that does not have a strain or a composition applied.
  • the "specified amount of contaminant' ' may be the level at which a product is deemed not to be sale for use by. for evarnpie. the FDA . in sotoe Instances, depending on the pathogenic microorganism, the specified amount of contaminant may he aero. This may be the case whet; the pathwgenic ndamwrganisro is listeria spp.
  • the specified amount of contaminant may be less than about iOO CFL ' g o mi or less than about 10 OA * g or mh sach as when the pathogenic bacteria is e,g i f A ii spp.
  • the tears "reducing" in relatio to pathogen contaminant means that die level of pathogen growth and/or speed at which a product spoils ts reduced when compared to a control product to which no strain or composition has been applied.
  • the terms Avxluoe” and 'Ye ocmg” may be used Interchangeably w ith the terms “inhibit” and "inhibiting. ' '
  • the degree of identity of genetic sequences can vary, lit s e embodiments, she variant has at least sy3 ⁇ 4y ⁇ > ⁇ >%, 5%, 96%, 97%, " - or 99% identity of genetic sequences with the disclosed strains using RAPD- PGR analysis.
  • RAPD analysis can be performed nsang Ready-to-Go' '" RAPO Analysis Beads
  • o cb are desipted as preornlxed, pre-drspensed reactions for per form bg RAPD analysis.
  • synergistic effect is understood to refer to that defined by application of the Tammes oosahod, ⁇ : I - ⁇ M, L. y Asohobs, a graphic representation f synergism in pesticides". Nether!. I. Plant Pathol. 70. 1964).
  • Ayoergstse effect is also understood to reter in particular to that defined by Colby ' s formula (Colby, S, R., "CAlculatlng synergistic and agtagonisiic responses of herbicide combinations". W eds, I S. pp. 20-22, ! 96
  • spores are boable " whet) they are dormant and capable of germinating.
  • the disclosure relates to bacterial strains, compositions and methods of using the strains.
  • the disclosure relates to isolated bacteria! strains, compositions and methods to inhibit plant pathogens. Including but not limited to bacteria, fungi and nematodes.
  • the disclosure relates to bacterial strains, eornposbona and methods for improving, plant peA cons mo -
  • the disclosure relates to bacterial sRains, com osi i ns arid methods lor impreeeing plant vigor.
  • the disclosure relates Pa bacRrial strains, compositions and methods for Improving plant yield.
  • the disclosure relates to bacterial strains, compositions and methods tor lobhoim: plant pathogens, na amather embodiment, the disclosure relates, to baciersaf strains, compositions and rnethods for inhibiting plant pathogenrc bacteria- in yet cm tiier e bo iment, the disclosure relates to bacterial strains, com ositions, and methods tor inhibiting plant pathogenic fungi. In another embodiment, the disclosure relates to bacterial simitis, compositions, and methods For inhibiting nsma tsdes.
  • the disclosure relates to bacterial strains, compositions and methods of using the strains, in one embodimeno the disclo ure relates to a composition comprising at least one bacterial strain disclosed herein.
  • one or more bacterial strains disclosed herein inhibit plant pathogens, including but not limited to bacteria, i ' ungi and nematodes.
  • one or snore bacterial strains disclosed herds improve plant performance.
  • am or more bac-enai strains disclosed hereio improve plant vigor.
  • oas or snore acterial strains disclosed herein improve p!sui yield.
  • the bacterial strain' s ⁇ are selected from the group consisting of: Bacillus !ti !enqulBick , Bitc ks s dBix, : ⁇ ⁇ .; ⁇ . ⁇ 3 ⁇ 4 ⁇ ikden or k, u*td "ami bad!i pofywyxa, and
  • the bacterial strain(s) are Baeill imtyBBHnBfacm selected tY m ibe group c nsisting of; oa'i is at BiqvBBcie BS27 : BadBus a yloiiqtqhckm BS203 ⁇ 44, Bacillus ryk&quifii kM ⁇ 5 ⁇ 4, Bacillus yBBiquBhciem AIM.
  • the bacterial stfa»s(s) Ba Hw Bchedfarmis selected from the group eonshahsg oft Bacillus Bchmi/or k BA842 : and Bacillus ikkcni/bcmk BL21, and combinations thereof
  • the bacterial strain Is Sacittm subtilte 3BB5.
  • the bacterial strain is selected from the grou consisting of: Badlhx amyloBq BBcism BS27, Bacl!iu* smyloBq ifac m BS2084, a illus amyhiiqiBB cm I5A1H B dBm umyioBqmfBck 3APd, BadBm iykBiquBack LSSAOI, ofo Bic >mBBiq fack ⁇ s ABP27S, BadB a yhBqcifaciem It) 13, Bacillus amykBiquifcKHm 91 S, Ba lha a ioBq ckns 22CFKand BacHim amykBqui/ ck BS I « > Bad lias BcBe formls & ⁇ BaaB ch ifof is BL21 Bacillus subBlk 3 BBS, and B e b lBm
  • the inventors ha e A and av.n one or more pams of Bacillus amyioiiqmk iercs have activity against p sst pathogens, including harmful bacteria, fungi and nematodes.
  • the inventors have also found that one or more strains of Bacillus amyioBquilack sun improve plan performance.
  • compositions comprising one or more strains of Baeil!m amyioiiqiB/uek are active against plant pathogens, including harmful bacteria, fungi and ⁇ nemaodes and that compositions comprising one or more strains can increase plant performance.
  • B iluA BSs 7 has aebvhy agans plant pathogens, inclnding harmful bacteria, fungi and constitutionafodes- I bo inventors ha- ⁇ . v abo f und that ,3 ⁇ 43 ⁇ 43 ⁇ 4S a;y 00 ⁇ iibotoiiy BSs / can improve? g!aai perfoonariee.
  • fisc mvemors have als f un that coniposbiona comprising Bc&kii s are active against plant pahsogens, brehidirig harmful bacteria, fursgs and nematodes and that compositions -oorgva-ong the strain can improve plant periommrice.
  • myhki ui faciei BS27 is nserib in the compositions and methods described and clamied heren
  • Thsa in estors have also found thai compositions comprising Badiius a yhhqatfydem 882084 are isefvve against plant pathogens, ineioding harmful actei , fungi and nematodes and that compositions comprising the strain can improve plant performance.
  • the inventors have found hai Ba ik yb:ki uift.; k?N. 15AP4 has activity agai st plant pathogens, moindmg harmful bacteria, ku i and nematodes.
  • the hwenrors have also fomm that ⁇ !,.:- ⁇ ⁇ >.-. ⁇ o;gi3 ⁇ 4j//g3 ⁇ 43 ⁇ 43 ⁇ 4:/of?s 15AP4 ears ireprov « pan performance.
  • compositions co prishsg Ba -fins I5 P4 are active against pla t ath ge s, including harmful bacteria, fungi and nematodes and that wmposit ss comprising the str in ear ; improve plant pcrtormarioe.
  • mtylotiqtdfius s ISAP4 is usefbt m the com posiio s m4 methods described and cianiied herein.
  • Sadttm m ioiiq fiKim I5AP4 was deposited at the American Type Culture Collection tATCC ⁇ , !OSO? University Blvd... Manassas, Va, 20 ⁇ 0 -2209 and given secessi n numbers PTA-6507. The deposit was made under the provisions ef the Budapest Treaty on the iniernafenaJ Recognition of the Deposit of Microomanisnvs for She Purposes of Patent Procedure.
  • the inventofs have fonnd thai BacUim amyiosiq lfack 3 P4 has activity ag inst pant pathogens, including harmtwl bacteria, fungi and nematodes.
  • the inventors have also found that Baa ' itw wvioiddf c ns 3AP4 cars improve plant erf rmance.
  • the Inventors have also fonnd thut compositions comprising i3 ⁇ 43 ⁇ 4r;/te amyioli ifixie ⁇ s 3AP4 3 ⁇ 4re active against plant pathogens, including, harmful bacteria, fungi and nematodes and that compositions comprising the strain ca» improve plant erfor a ce.
  • any derivative or vsriaot of BadJhi ⁇ > amyfoiiq iaekm 3A.F4 is also included and is useful in the compositions and methods described arid churned herein..
  • B dtfm amykiiqi-dciem LSSAOI has activity sgajnsr plant atho e s, including harmful bacteria, fungi and nematodes.
  • the inventors have also found x ⁇ m B cill LSSA I can improve plant performance.
  • compositions eornpmmg one or m e strains of B d-Hu a yhiiq hckm LSSAOI are active against plant atho ens, including harmful bacteria, fungi and nematodes and thai compositons comprising the strain can improve plant performance.
  • m LSSAO! is included and is useful m die -:-:-- ⁇ ' -B; s and mei ods described and eiaimed herein.
  • the inventors have found thsiBmUlm cimyiohm csei-ss ABR278 h s aeuvltv against plans pathogens, moludhrg harmful bacteria, fungi and ema odes. inventors have also found thai BociB im oo «a(aa ;e «v ABP278 can improve plant performance The inv ntor?
  • compositions comprising .B cili Qmyfoliquifacrexs ABP2?s are active uao-oo plant pathogens, including ha mful bacteria, fungi arid mamttodes and that compositions comprising the strain c*w> improve plant perArroiUies.
  • any derivative or variant, of BacBha a kiigui/acktis ABF 8 is included and is u eful in the comp sition and methods described and clamed herein,
  • xhttBtMnHm amylGiiqitmciens 1013 has activity agamst plant pathogens, including harmful bacteria, rungs and riemasodes.
  • the inventors have also found that &3 ⁇ 4o! «s 10] can improve plant performance.
  • the inventors have also found that
  • eontpositions comprsing Jft?ci//oo ose!.3 ⁇ 4ooey3 ⁇ 4ioe;3 ⁇ 4 1015 are active against plant pathogens, including harmful bacteria, fungi and nematodes and that compositions corn prising the strain can improve plant performance.
  • any derivative or ⁇ :> ⁇ "> ⁇ of teuore a ykUquifac s 1013 is ineirafed and is useful in the compositio s and mehods described and clamed herein.
  • a st afe havmg all the identifying characteristics otBaciUm amyh i dhekfss 1 53 is useful in the compositions and methods described ami claimed herein.
  • the inventors have found that /o dgo .» ioi;quffaci& 918 has activity against plai , pathogens, including harmful bacteria, fungi and nematodes.
  • the invenrors have ab found thai BaetU ro'fnaoa7i/do/oss 91 $ can i pre-ve plant performs nee.
  • the inventors To e also found d-.o composlnoiis comprising /to- a/ to o y!ohf ihcwt-ix 918 ssre acti e agsmst plant pathogens, including hannrid bacteria fungi and nematodes and that compositions comprising the strain esan improve lans erf nce.
  • any derivative or variant of i3 ⁇ 4s-:h/u « 91 S is also included and is useful in she com ositions and methods described and claimed herein,
  • the inventors have fom that Bacili oia-/o2g o/o-;2so 22c T7 has activity against plant pathogens, including harmful bacteria, fungi and nematodes.
  • the inventors have also found that BwMhm a yioHq ui/ aen ⁇ 22CPI can Improve plant performance.
  • the inventors have also round that com postions comprising Baciii cmndoHqidf iens 22CPI are active against plant pathogens, including harmful bacteria, fungi and nematodes and that compositions comprising the strain can improve plant performance,
  • a strain having ail she identifying characteristics of Bacilfes a yhiiQiiifyckns 22CPI is useful m the compositi ns sod methods described and claimed herein On ten. 1 A 2005, ik 'ihtv eyfofA s depoaked si s Ameman T pe Culture Collection ;ATCC), iOSOi Universit Blvd., Manassas, Vs.20110-2209 and given accession number PTA-6501 220 " !). The deposits were made tmder the provisions of the Budapest Treaty on the Internationa! Reoognidon of the Deposit of Mreroorga.nismsd r the ⁇ > of Patent "o- odnro
  • the inventors have aUo found that Ikidiin- «»n oo/npgio0;3 ⁇ 4. ⁇ B Ss can uis rove plan* pe fonnaoee.
  • the inventors have also hound dsat compositions oon i aag acUi-m !fo ) x are active against plant pathogens, inchming harmful bacteria, fungi and ⁇ « ⁇ 3 ⁇ 4 ⁇ ! «8 and T ai com ositi ns comprising de strain cars improve plant P'-oiooneno.'
  • any derivative or- vananteTA vV3 ⁇ 4 mnyiokquBBekns S ⁇ is ho- -Toed and is useful in the compositions and methods described and claime herein.
  • com ositons comprising one or sn re strains fM if!us P ' vegpovoo" are active agaimd plant pathogens, including hnraful bacteria, fungi and nematodes and thai compositions comprising the strain nan improve plant performance,
  • compositions ⁇ ,'o;a:a vdag Bacili ik e kbr k BASd2 are aeli e against plant pathogens,. In luding harmful bacteria, fungi and nematodes and that compositions comprising the strain can improve plaes perfbttnance.
  • v a ⁇ dam su> 3 ⁇ 4;sy deH aUvc or variant of Ar?cA;A? vs-o;a.yoo BAadg Is ne.dm: A and A a.oTn « da compositions and methods described and claimed herei
  • a srain having all the klendfyirrg: characteristics of Baciiiw !ichenifyrm!s BA Z is useful in ids compositions and meth ds d scribed ; ⁇ ; ⁇ a claimed herein.
  • the irrsentors have found thai Bacillus tiahedfor Hi id has activity against plant pathogens, including imrmtoi bacteria, fungi and nemat des.
  • the mvenUas have also found that Baciir
  • Ae/sra/Ae-scA B.L21 can improve plant perf rm ⁇ nee.
  • the inventors have also found that coinposksom comprising Baciiiw; iic nn rmis 8L 1 are a t e against plant pat ogens, including harmful bacteria, fangs and nemato es sad that compositions comprising the strain can improve plant performance.
  • embo iments say derivative or variant ⁇ > ' A.-Ab;.v ikki > ?nibr!>us BL2! is also included and is useful i the compositions and methods described and claimed herein.
  • a strain having all die identifying cfmraotensties of Bacii!ii* li henuhr is BL2I is useful in ihe coinpositions and methods described and claimed herein,
  • the inventors have found that one or more strains of Aoc///3 ⁇ 4s aafo/IA have aePAy against plant pathogens, imduding harm hb bacteria., ;o .a and nematodes.
  • the inventors .have also found diet one or more strains f Aradfo vrAidA can improve plant performa ce.
  • the inventors have also found da s. compositions comprising one or more train ⁇ of BudJius savdA
  • compositions comprising the strain can improve plant performance.
  • a strain having ail h identifying characteristics of dihis suhti .$ 3BP5 is useful in the compositions and methods described and etaiated heroin.
  • the inventors have found that one or more strains of P enibsi'ii a paiy yxa have activity against plant pathogens, including .harmful bacteria, fungi and nematodes.
  • the inventors have also found that one or more stnnns of Pnefifba iikis p ly yxa can improve plant performance.
  • compositions c m osing one or more strains of .fba:on bacp/ae cyjagrvo are co e against plant pathogens, inckrdlng hanrbvd bacteria, fongi and nematode*; and that compositions comprising the strain can improve plant performance.
  • the inventors reive found thiU « ⁇ Ate/ a.v po m- ⁇ AB I 66 has activity against plant pathogens, indudmg harmful ba teria, fungi and nem todes. The inventors have also found that
  • compositions comprising one Of more strains of ' Pxe?nbticfik& pabymy ABfobtS are active agauvst plant pathogens, A:hkbsg harmful bacteria, fungi and nematodes and oca eomposUKms eomprisinp the strain catt approve plant performanee.
  • any derivative or variant of P ntb&cltim polymy ABF 166 is also included and is useful in the compositions and methods described and claimed herein,
  • poiymyxa ABFI 66 is useful In tbe compositions and methods described and claimed herein.
  • Paenib ciH-ii& ⁇ polymyxci strain ⁇ was deposited by Danisco USA. Inc. of Waukesha, Wisconsin at the Agneuli a! Research Service Culture Collection (IbR L , 1 SI5 fsortb University Street, Peoria. IH,, 61604 or; December IS.2008 under accession number B-S9211. The deposit was made under the po isi ns of rhe udapest dreary on the hnernntional t ⁇ eP nbon of the s.Wrt n ⁇ ; Mic oorganisms for -he Purposes of b nets Procedure.
  • Cope hagen Denmark to refer to these eposited biological materials n this; patent application and have given unreserved and irrevocable consent to the deposited -maoriA being made available to the public,
  • the catering can take place with, on. or In the presence of one or more subsrates (e.g. n fermentable substrate), A fermentable substrate Is materia! that contains an organic compound such as a carbohydrate teat e r; be transformed (eye, convened imo another compound i try Ac eneyrnarie stetson of a bacterium as disclosed herein.
  • one or more subsrates e.g. n fermentable substrate
  • a fermentable substrate Is materia! that contains an organic compound such as a carbohydrate teat e r; be transformed (eye, convened imo another compound i try Ac eneyrnarie stetson of a bacterium as disclosed herein.
  • substrate examples include, but art; not limited to, non-fat dry milk, vegetables (e.g., com potatoes, cabbage), starch, gmlss fe.g., rice, wheat barley, hops),, fruit (e.g., grapes, apples, orsrrgeO. sugar, sugarcane, meat (e g., beef poultry, pork, sausage;, heart infusion, cultured dextrose, combinations thereof, and the like and suitable media containing proteins, carbohydrates, arid muterals necessary or optimal growth.
  • a nor lm using exemplary medium is ' FSB- or CASO broth,
  • the substrate may include one Of more of starch, soy. yeast extracts and salts, in set another embodiment, she growth medium may be CASO broh, in still another embodiment, the growth medium ma be ⁇ 8 ⁇ broth,
  • the euh4rrlng of the bacterial strains disclosed herein can take place for any suitable time.
  • euHuring the bacterial strains cars take pla.ee for a period of time conducive to prodocc a compound of Interest.
  • the catering can take place from aboel S to about 72 hoars (h). from abom 5 to about 60 h, or from about 10 to about 54 h or from 24 to 8 h.
  • the ce!mnng cars suitably Oke place for about 1,2, 3, 4, 3.6, 7.
  • the rime for oohtrring can be greater than or equal m about i, 2 : 3,4, 5, o ?, 8,9, 10, 11, 12, 13, 14, 15, 16, 17.18, 19.20, 21, 22, 23, 24, 25.26, 27, 28, 20.30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41 , 42, 43, 44.45, 46.47, 48, 40, 50, 51, 52, 53, 54, S5, 56, 57, 58 5 59.60 h.
  • the time for catering can be less titan or equal to about 1 , 2.3,4, 5,677, 8,6. Kg 11, 12, 13, 14, 15, 16, A.
  • she yahnnng occurs for approximately 20 a - 30 hoars
  • she yahnnng occurs for approximately 20 a - 30 hoars
  • Use temperature daring the euiiuring can be from about 30 ro about 55 a C from about 25 to sboal 40 %2, or from about 30 to about 35 2 In ne as ect the temperature during the ouhur g -an be iron) about 20 0:; ahead WC from about 30 to about 4irC2 or from about 40 to about 50%2
  • the enkuri g arm take piece a?: a temperature of about 20, 21 , 22, 23, 24, 25. 2b, 22, 28. 20, 30. 31 , 32, 33, 34, 35, 3d, 52, 38, 39, 40, 4 L 42, 43, 44, 45, 402 4 ? , 48, 49, 50. 5 h 52, 53, 54, r 55%2 where any of dee stated values can form an upper or lower imdpoint when appropriate. ?n still anofber ernbodimenh the eulturing can take place at a semperalare greater than or equal io about, 20, 1 , 22, 23, 24 , 22. 26, 27, 28, 22.
  • the euJtaring can take place at a temperature Daa than or equal to about 20, 21 , 22, .23. 27 25, 20, 22, 25. 20, 30, 3 L 32, 35. % 5 35, 36, 32. 3a, 39, 40, 4 b 42. 43, 44. 45, 56, 42, 41, 49, 50, 5 i , 52, 53, 55. or 55°C.
  • the eulturmg can occur from about 30 to about 3S a C.
  • the ceHuring can occur at about 327 2
  • she cidtormg preferably may take place under aeration le oue embodiment, the level of the aeration is controlled.
  • Aeration levels may be expressed as dissolved oxygen tension (POT), w some 5; DOT is a percentage of oxygen saturation in the culture, fog. ! 0P3 ⁇ 43 ⁇ 4 DOT means a cul ure is fui!y saturated w th oxygen). .DOT may be measured as taught &srosn er ⁇ .
  • the le el of the aeration is such that the oxygen content io the culture is more bum about 200, DOT, more than about 30% DOT, more hat! abo «M0% DOT, more than about 50% DOT, more than about 60% DOT, more t m shout 7055 DOT, more than about 8055 DOT or mo e than about 9055 DOT,
  • the level of the aeration in die -uhmro is abom ! OOob DOT-
  • da; level of aeration is such that the oxygen con ent its the euhure may be between about 255b and 505a DOT.
  • the aeration may he pros Dowred b any suitable raedu -a.
  • mibodlmmn > ti m i «a «y by profited fey any m «&ns th&i mhm with the mfom.
  • the aeration naay b provided by agitation (e.g. shaking, oscillation, stimng etc, ⁇ or by passing air (eg, oxygen) t rou the culture m&dls, for example, or eo b ation thereof.
  • vv the volume of gas per liqu d volume per minute
  • vv the volume of gas per liqu d volume per minute
  • the aemtioo rate mm be Is the range of abend 0, 1 to about 0 vent.
  • the aeration k provided by agitation e.g. in a stirred ierrneoror
  • the aeration r te may be in the range of about Cb l to about 3 vvm.
  • the culture media e.g. In an airlift iermenlor
  • the aeration rate may be In the range of about 3 to about 6 vvm.
  • a culture container that is desi ed or shaped to support or provide aeration may be used.
  • the culture container may comprise one or more bailies, The aim of the bailies may be to encourage ex osure of the media to oxygen (cyy air ⁇ ,
  • a culture container with baffles may be used in combination with shaking or oscillation of the culture container.
  • the culture container may be the container described in V.S. Patent Mo, 7.381 ,559 (the subject matter of which is incorporated herein by reference).
  • the culture mediant rosy be agitated.
  • This ntay be alleoted by amy conventional means.
  • agitation of a eoiture medium may have a number of beneficial effects when compared to a non-agitated culture medium, including but not limited to: increased growth and/or decreased cell clumping and/or increased nutrient (e.g. carbohydrate) mixmg and/or tetter nutrient distnbntkm aud/o? Increased protein production and/or increased primary metabolite production and/or Increased secondary metabolite production etc.
  • nutrient e.g. carbohydrate
  • the beneficial effects derived from agitating a culture medium may result from the creation of turbulence withi the cubore medium bet-', by stirring), in one emtedimeog the agitation may be stirring. In another embodiment the agitation may be shaking or oscillation.
  • the culture media is agitated by oscillation (e.g. by rotatory shaking), in another embodiment, die speed of rotation tmy be at about 5b to about 250 rpm, about 60:epro to about 240 rpm, about 7b rpm to about 230 rpm, about 80 rpm to about 220 rpm, about SO rpm to bo t 210 rpm. or about 00 rpm to about 200 rpm,
  • dtc- speed of rotation may be at about 100 rpm to about 1 50 rpm.
  • the speed of rotation may be at about 1 0 rpm.
  • tire culture medium is agitated in order to Increase the level of aeration in the culture media and/or increase nutrient mixing in the culture media. It has bees found thai aeration and or agit&iidr* of the culture mixture may result in significant im r ve ents in the iermentase produced . Without wishing to be bouttd by theory, da:- invpowv ent may be paused by ensuring the ceil denrity or cell mass in the outers container is such that the protein yield and/or primary metabolite production by the bacteria is enhance in the consumermai3 ⁇ 4.
  • the eu!mre media may be agitated by stirring.
  • the speed of stirring may sui ably be greater thai! about SO rgm, for example between shorn SO rpm to about 1200 yarn
  • the rate at which the culture media may be stirred may be dependent upon the container in which it is held for caterin pu poses if the eoat&iner comprising the culture media is a small fermentor (s.g. less i 500 L, such as aboot 100 to abof.it 500 L or even less thar! 20 L). then the speed of smring may be at kmw about i OO rpm io about 12 rprm tor example.
  • the speed of rtirrmg may bo greater bom about I 200 rpm, if the container com rising the eohure media is an industrial scale fermentor (yog, greater than 500!.,, such as about 500 to afoo 20,000 I,)., then the speed of stirring may be a least about 50 rpm to abou 150 rpm or may be greater than about 150 rpm, for example,
  • agitation of a culture media during entering may be iv/preseoted as po e input by agitation, ? ⁇ a -r input by agitation w a sepresentation of the amount of energy prov ided per Oier of hqaid volume.
  • the power ingot by agsPdion cars be calculated by fOss determining the power in awyoa using the follwvjog tonrnda:
  • M 6 is a dimensioo!ess number /Newton number
  • p is the density of the liquid (kg/nr)
  • N (s " ) is tite rotational frequency
  • D is the impeller diameter (m).
  • P t . k the power drawn by an agitator when the culture is not aersted. O-uwuabwi of p was hq>m by aoawts -n in the presence of aeration is iaagh? ia Ohnos et o/.
  • the power ingot by agitation per volume may be at least about 0,25 fcW/nr .
  • power input by agitation per volume may be in the range of about 0.25 kW/nr to about 6 RW/mO fn
  • the power input by agitation per volume may be In the range of about 0.25 h o.
  • the culture volume to the container volume may be less than about 1 : 1 v/ ' v, e,yy 1 :2, i :3, etc.
  • the ratio of the endure volume to rhe container volume may be less than about i : l v/v, 1 :2 v/ ' v, 1 :2 v/vy ⁇ ⁇ v/v, ) v/y 1 :6 v/v, 1 ;7 YW 1 : 8 V/V, i :0 v/v, or 1 ; 10 v/v.
  • the ratio of the culture volume to the container volume may be in the range of about i oi v/v to about 1 : 10 v/v, so one aspect, io the raoge of 1 .3 v/v to about 1 : / v/v.
  • the ratio of the culture volume to the container volume may be about 1 : 1 v/v, 1 :2 v/v, 1 :3 v/v, 1 :4 v/v, 1 :5 v/v, I ;6 v/v, i 2 vwy 1 :8 v/v, 1 :0 v/v or 1 ⁇ 0 v/v. in a»0ih ⁇ r em odinrsnt the ratio of the culture volume to she container > au c may he -boo; ! :5 v v.
  • the volume of the culture may bo in the range of about 60% to about 90% that of the container volume, for example.
  • the volume of the .. o!mre may e in the raoge of aboul 70% k; about 85% Ova of the container olume.
  • Tbo phi during the enlisting can bo at a pU f»i « about J to aboai 9, from about 5 to about 6, from about 6 to about 7, from about 7 to about 8, or ftom about % to about 9.
  • the eulturiog ears take place at a pH of about S, 6, 7, 8, 9. where any of tbo stated alues can form ars upper or lower endpomt when appropriate, in one aspcci, the pH Is at a pH between about 7 and about S, from about 7 to about 7.5, irom aboib 7.1 to about 7.3 during the cultming.
  • the euiturmg is at about pH 7.3 ,
  • the pH may be adjusted after cu!turing to a pH from about 6 to about 0, or from about S to about 10, or from about 9 to 10.
  • the t may be adjusted ' ⁇ ' ⁇ ⁇ -: about pfl 8 as a ut pH 9, n;
  • ihe pl i may be adjus ed to about pH :>.
  • an alkali may be used to increase the pH.
  • otassium hydroxide KOH
  • KOH otassium hydroxide
  • the pH Is adjusted aber separation of the bacterad cells and culture media .g. by eenrriiugation ⁇ .
  • ho;- pf l of the supernatant is adjusted.
  • the eu!tarmg step c m rises one or more adjustments of the cult ure conditions isueh as an adjustment of pl . temperature a d/ or substrate) during the eabunng phase.
  • adjusting t e culture eondhions io.p, pfl. temperature and/or substrate) during the euitursng may increase the number of compounds of interest produced during the culturi g process.
  • the initial culture con itions may be conducive to produce one compound of Inter es and the adjustment of the cultur conditions may provide favorable conditions to produce a f i!ter eouipoimd of interest
  • T hus, tor example, urin the culturbsg process an initial pH of about pH S may produce one compound of interest. Subsequent adjustment cf the pH to pH 7 during the same cu I luring process may result m the production of further compound of mprest.
  • a fr rnent oon product of the disclosure or a portion thereof comprising eoR>potmd(s) of interest may be prepared using batch or coodsusous cbtur pg.
  • a iemtiint&noe product of the do «,.:o:oo is harvested dnriae or at the end of the exponential phase
  • the fsrmers ksn product ofthe present invention is harvested or dining the stationary phase.
  • the consumeraoirion product may be produced in a vat under commercial condlliooss.
  • bMtoHai annas dlsaiosed heosan re o hnrod to between about a I a ⁇ ( ? CFU/ro! i about a. I x 10"' CPU/mi.
  • the bacterial strains disclosed heieht can be grown in Tryptic Soy Broth (TS.B), Milk Based Media, or Optiivdeed industrial Media do Man, R gosa ri Shsrpc ( MRS) broth at 32 i3 ⁇ 4 C for 24 hours.
  • the bacteria can be harvested by aermafoaailoa, and Pe snpernateist removed.
  • t e hoPorna- relates to composition comprising Of eorubstnus of or eomisPng e ⁇ semiaily ot one or more bacteria! series
  • the composition comprises, consists oP or consists essentially Aba; ⁇ or more ofbseienai strains selected !torn the group aoesisting of: FJa>ciihis Oiop/ popac/efte BS27, Baalim BS20 . Itaciii amyiaaqaqackm 1 AP4, B ciiiw aciyiaikpsifackns ' ⁇ ⁇
  • the disclosure relates to a composition eomprlAog at least two baeteHal strains; selected the group consisting of; Bacili&s amy isquifack BS ' a AaciMo?
  • she disclosure relates to a composition
  • a composition comprising at least three bacterial strains selected from the gro >. u a t ⁇ T L
  • the omeaours relates Pa a e3 ⁇ 4mpessbon comprising at lcs-a t ' oor bacterial occur the group consisting of: boorno m B uifixiexs ES27. acsUus ⁇ yioiiq; f ci s BS2084. Bac/!!m amyioHqt hcie !
  • t e di closure rdato its c mpositio comprising die bacterial strains dscosed herein and one or more com ounds or agents selected from the group consisting op
  • agcoclmmically active compounds bloeofitroi agents, lipo-chevoobgosaechadde compounds (LCOs), isoflavones, oamiazolines, insectlddal compounds, aaolopyrlmidinyi mimay polymeric compounds, ionic c m und, siibsniuied tbiophenes, substituted dithiines, fluopyjrarom,. ena inocarbonyi compounds, strigolactone compound, and d ith Bnc-tetraearboxi sdc compounds.
  • the disclosur relates to the use of a first e-mnposjuon comprising >hc bacterial strains disclosed herons and a second composition comprising one or more compounds or agent; selected from he group consisting of agroehemically active compounds, biecoao-oi agems, iipo- ohiioobgosaeoharide oornpotuKb i!XOsp koflavones. quws3 ⁇ 43iines, msectickla! compound,
  • azoiopynniidlnylamine polymeric compounds, ionic compound, substituted tbiophenes. substituted dnbilne nuopymtnm, enaminooarbonyi compounds, atngolaetone compound, and ditbiioo- ietr carboxiralde com ounds.
  • the disclosure relaes to the use of a first com siio co rising o e or m e bacterial strains disclosed heroin ami one or more compounds or agents selected from th ⁇ group consising of agrochemioaliy active com unds, blooonirel agents, bpo--ohitooiigosacebande compounds (LCDs), isofiavones, qumaaobnes, inseetioida] compound, azolopynffik!iny mine, polymeric compounds, i nic compound, substiuted truophenes, snbsuttaed dithenes, ilnopyranmo ens-ninocsrbonyl compounds, strfgolactone compound, and dthino-tetra aitoxlmkle compounds, and a second composition comprising one or more compounds or agents selected from the group consisting of:
  • agroehemmaRy active compounds bioconfro! agents, sipo-chiio !igosaccharide compounds (LCOs), isollavones.
  • LCOs sipo-chiio !igosaccharide compounds
  • isollavones qsnna oiines, seoticidai com ound, teolopyrimWinylamine. polymeric compounds, ionic compound, substit ted tbiopbenes, substituted d ith sines.
  • flnopyfamm. en&m i ivocarbonyl com ou ds, strigoiae>one compound, and dnhbne emea mmide compounds.
  • enandnoearbonyl ompounds, sirigoiactons c mpo nd, and dldbltxotetracarhoxim le com und* or Use KomerP according to the disclosure with the at least one bacterial strain and st least one compound referenced herein can be underst od as m g a physical mixture of the bacterial simSn and ih « vompoutHi.
  • the comb ned use may also co sul of usin one or more bacteria] strains disclosed herein and at i ; ssi one compound referenced herein separately, in yet another eoibodhoeni, die bacterial strain and compound may be used ithin a suiTleiemly short time of one anodser so diss the desi;3 ⁇ 4d effect can take place.
  • compositions disclosed herein improve plant performance or plant vigor beyond the Improvement observed ith die active com ounds alone (synergism).
  • the action of a eomposnion disclosed herein goes beyond the action of the achvs compounds present io the mixture alone (synergists).
  • azoiopyrimidinylaoune polymeric compounds, ionic compound, snbsduued rhiophetres, substituted dithiines, floopyrantm, enamiaocarbonyi conrps3 ⁇ 4onds, sirigolawone eott ound, and dnhibvo- teimearboxlmlde com ounds ellosvs enhanced control of pa!hagnes, harmful pests, sacb as harmful fungi, mseots, or anirnal pests, compared to the control rates that arc possible with the individual eompoe-nds (synergist c mlvinres).
  • the joint, separate, or successive application of the haeterlai strains disclosed herein and one or more one agmehemieaiiy active compounds, blcKmaPr ; agency lipo- ehlsooligosacoharide compounds (LCOs), is ikvones., qumazolines,, Inseeticrdai compounds,
  • axotepynmidlnykiTOiiCS polymeric compounds, ionic compounds, substituted tblophenes, substituted dltlblneoe fluopyfa m, eosmtnocarboayl compounds, strlgolaetone compounds, and dilhilno- ebaearbosJrnide compounds provides enhanced plant health effects compared to $b « plam heakh effects that ate possible with the individual compounds (synergistic anxun, wherein Pie synergism is lant health synersgismy
  • rhe disclosure re lams to a composition comprising one or more bacterial strains disclosed herein and one or more bioeontrol agents.
  • bioeontrol agent C ! €A*) moHioAs ono or m re aoAA , frnagi or sastss, p? km>ss, i uses, enario> ttd-a no nemat deR, and boiamcal extrseiy or products roduced by tnieroorgsnisms including proteins or secondary niUefr dp. ⁇ and ⁇ >. aUuA that have one or both oi «.
  • dipiopods, or thai inhibits plant infestation asKo i- growth of a comb iaiioa .; plaas pat og ns; (2) n spr es plan: performaace; (a) boproves plant vAkl; (a) m r es plsai vi r; and (5; improves lans health,
  • NKRL B- !820rA Bacillus ihu lngiensA subsp. teneferioms sn-ain MB J 76 (products known as Novodor "' l-C ' from BioFa, DB) or Bacillus tlnuingicnsn subsp.
  • BunirnyJensis strain BD#32 (Accession Ho. NRRk B-21530) from Agmquest, or Bacillus thurtngl «3 ⁇ 4sis stratn AQ32 ( Accession Mo. NRKL Bk* 1619) from Agmp, «esk or Ba ilas thanraAensls strain CR--371 (Accession No. ATCC 55273), (CI, 28) Bacillus wnif agdlate, 3 ⁇ 4Sftia acidovofarss, m particular strain R Y200 (products; known as BioBoosr), 1. w docao- snbblobcus, in particular ar,un 13-1 ⁇ ⁇ .1.
  • pn lu-.' known as Proradix ⁇ y Sraptoarvces galhus, in particular strain K61 (Accession Ho, DSrvl 720ft, products known as ycostop', cf Crop Pr moton 2006, 25, 468-475) or strain ⁇ , ⁇ 30232.
  • Siroptorayocs grn:eovsridis products kn n as o ycostop * ).
  • Bacillus aoldotcrrestAs Bacillus aoldotcrrestAs, (Bacil!os iastldioscrs, Bacillus aio atertuni (products known as Bsoardy from Bo/Are), or wain YPM3.2S, Bacillus psyclntssaccharolyticus. Bacillus nmroccanus. Bacillus inestatcriaiu C4 Bacillus pankuhemjcns talso known a; Vdrydascilios psntobicndcusy in pariietdar strain Al ' CC 14576/ DSM s ' dk Bacillus lentns.
  • Pseudonaonas cepacia (ex Burkhoidoria cepacia, roduct known as Deny frorst Stine Microbial Products), Bacillus nernatocids, n particular strain BA6, Bacillus cireuians, BrewibaciHus ErtoroKporas (also known as Bacillus laicrosporus), in particidar strain A Bis: da or train MRS 1111 or strain NRS 1 S or strain MRS 1647 or strain BPM3 or sinbn 04 or strain NCIMB 1419, Cor nebacicri una paurometsbokuty Eaciobacilitss acidophilus (producis known a FnltsarC front iaagrosaBndualnas Agrobioiogleas, S.Ak Paoslbacilkw ⁇ .
  • prodtK is known as Biooem, VO ' T ' IVO), Ba illus cereus fsvnonyms: Bnedius endorhythmotu Ba dlas medusa), in particular s res of Bacillus ceo-n strain CNCM i - ! 362 ( f. US 6,406 A90).
  • strain BPO i ATCC 5S675, product known as Mepichior from Arysra, US or Meppkrs, Micro-Flo Com an LLC, US
  • prodncu known as BacsiarU d ons I: ⁇ .; Crop SoiulioiW, NZ, or Double NickelTM [Ann Certis, US), Bacillus' sabtilis. in particular strain GB03
  • strain QS1 3OO02/AQ30O02 accesion No. NRR.F B -5042 K ck W(3 20 i 2 '087980 ⁇ or strain QST30 04 AQ3000 (Accession No. NRRL B-5045S, eF WO 20127087980), Bacillus purnilua, in particular strain C3B34 (Accession No. ATCC 700814 , products known as Yield Shield ⁇ iron? Bayer Crop Science, DE) or strain QST2S08 (Accession No.
  • NRRL B » 300S7 products known as Sonata QST 280 ® Bom ⁇ uoA : -w- o US), or strain Bo F-33 ( r duct known as integral F-A.3 Bom Becker Underwood, US ⁇ , or strain AQ7 I 7 ( Accession No, NRRL B2 I 662, Pasteuria sp., in particular strait) SD-5132 and Fasteursa sp,, in particular strain PTA-9G43, Agro- bacterium nrdiobaencr.
  • Bacillus a!veg B&ciliws armnoglucaskiic «s. Bacillus mua:anmiig Bacillus anryio!yticirs (saw known as Bae?Bbiaa!hi « aaiylolybeus). Bacillus attoinT oiybena Bacillus snbtills isolate B246, for example in form of the commercially available product Avogreco Boa ; RE at UP), Bacillus taqailewsis.
  • the BCA is one or mora spore forming bacterium, in amaber embodiment tbe spore forming bacerium is selected bom the group c nsistin of: spore- forming baeterhtrn (B) of the genera Bwiikts is selected from the group consisting oB B cithtsfirmus m n CKC Ci >82 ; Bacillus; cereas -moo CNCM b-1562, BaciHws n > iiqmil; m$ strain lN3 ⁇ 4A7a, Bad/ii y iiq fa ie strain P2B42, AosAbo s bri ' Hs strain G803 ; AacbAne nAb/ strain OST713 ? AacBay /aarb/as strain GB.A. and Baca!w p mih strain QST2S08,
  • the BCA is a bacterial strain selected from the group consisting of In another enb3 ⁇ 4xhn ⁇ ont, the BCA furthermore products ased on Bacillus firmus ⁇ including bat not limited to strain CNCM 1- 1 582.. such sag fo exampkx VOtiVOTM, BioAom) Baeiikrs subnlb GB05 nd QST 713. Bacillus subbiA var. amyloiiqae3 ⁇ 4e1 ⁇ 2ns FZB24 and Baeiikss amyloBpusfaGcas FAB 42.
  • the fungi or ycgsts inhub bat are not limited to: Ampe!omyces q « 3 ⁇ 4 «&1i « s ia particular strain AO b (product known es AQ ⁇ ( f " h Atireo-basidinm palhbaas, in particular b!astospores of strairs DSMI4B40 or blastopores of strain DSM 149 1 or mixtures the eof (product kno n as Blossom ProtecGO, Reayveria bassisna, In particular srai ATCC ' M040 (products kn n as N- ⁇ ar- dd ' .; or sirain HA (products known as IGyc hM..
  • BoisniGyrd or strain ATP02 (DSM 24665 ⁇ or strain CG716 (product known as BoveMas; ⁇ .
  • Candida o!eophila in particular strain O (products known as N «xy* f ) or strain GIS2 (produces known ; ts ASPIRE*, Dceco GlrG), Coniothyriam rmndans, in panicabr strain COId/M/9 ⁇ 8 ⁇ S ' M- 9660) (products know as Comacs * ' ⁇ , Ditap ospho « alopccari (prodncts known as Twist Fungus * ), GHocladlurri oatenukmrny in pas teomr strain JJ446 (products known as Prestop w, Lecanicifiium lecsnu (rorsnerly known as Vmfci!ium locanii).
  • n parucu!ar aorddia of strain KVOi products knows? as ycotal® Vmafee* from K expert Arysta) or mn QAQMX9 99 or DAOM21(G9p, Met&rhisshsm anisopHay, in parikaodar strain F52 (DSM 3S84, ATCC 90448,.
  • Nomuraea Meyy ITS particular strains SA86UH, GUS74 I, S S6151, CO ! 28 and VAO!Ok .
  • strain DV74 products known a Folyversum
  • atram Ml ATCC 3H472
  • Triehodcnria acpordhrrn in particular strain ICC 0]2 (also known as Trichodorwna harGanum ICC012, products known as Bioten 3 ⁇ 4 ⁇ or strain S T-d (products known as TrldevrnaaP or ECOA-!OPE ®) or strain T34 (products known as T34
  • strain ARRL 21882 foduG kn ws as AilaAArard'y irons Syngentas or strain AF36 (product known as AP36A Candida sswaus. in particular strain GRRL Y--2 ! 022 (pro-ducts known as BKX3GRE® or BlOCGATAp
  • Triohod nna vkido also known as TVtchoderma ga sii >. in particular sirain ICG 080 (products known as RFPAEDfER 5 * Wr t BlodcrmaG and strain TVs (products known as ' vlrkls T k Agribiutec). Triehoderma harmatura, isolate 3S21 fickodettna koningd, Trtebodsrma Ugnoruay
  • strain BRIG products known as BOTRY-AFK!
  • TfteiKXii-rms gamsif products: known as BIO-TAMTM ⁇ or fa parbeidaf sta 1CC080 (products ; known as BbdonnaA U- ⁇ aeba ekrifor is, Maseodor rosoas srram A3-A A aabon o. NRRF 3054Sy
  • T polyrporam and Id haraanusrs o.g; Bird AS ® from BINAB Bio- I novation AB, Sweden
  • T. sitoniatieaat e.g. TRICOVAB* Id n C.ERLA.C, Brazil
  • U3 e.g. BOTEYLAKN* from Botry-Zen ti N3 ⁇ 4 in one embo im it, the UCA is is o mm profc&oas.
  • the BCA is a vims selected from tba group oonigsb ad) Cydla pomoraslia gramdosrs virus, Adoxophyes oraaa granulosis vbns, fkbeoverps armlgcta auciaopoiyhedro virus, Spodop3 ⁇ 4ra exsgua nncleopolyhedro irus, Spcdoptera IrAorails nuc!oopdlyhedr virus, Spodogtera Cru iiiicleopolybedi'o virus, Neodipion abietis nucleopoyhadro virus, and Naodiprion semfer aoclaop oybodm virus.
  • Heierofh&bditis rnaroA-ios Heterorhabdius ategidA, HeerofhabdbA aealaadAa, Phasnwhabdhis hem;aphredha, Stemeriensa bibioms, Sveinemerna giaseri (products known as Siotopia), Sieneawnia kraussea (products Aawso as barvesure, NeniasysA I,), $iej ««m«* «a nobrave (produces known as Bsoveotor) Siebaomosaa seapterisei (products known asNemitoae Sb Sieinem ira scarabaei, Stehteinem sianAayA, BeddAysa ⁇ TM Dedadersos) sirkbco!a, iAbpjevhnermis ASpsaadra, and Sidn
  • tbe BCA A one or mora mocoiants.
  • tn «t soother endsodbweat the irtoetdanrs are selected from the group 4 consisting of: Rbizobium J gyminosarum, pardcaiar bv. vjceae stram Z25 (Accession o, CECT 45851 Rhi bi m irop!ci : Rkisohhw kui, Rhizo&iwi tHfhlu, R sobiv eiii L Rkizobhiwfre ii. AixH- bium fsunfiodats, eudo on s, Azospiriihim./iz wbacler,
  • the BCA is one or snotx- botanicals.
  • the botanicals are selected from the yecep consisting of: Botanicals (or: plant extracts) selected front t e group consising of Thymus oil, A ⁇ adirachtin (Neetn), Fyrethi'&m, Cassia nigricans, Quassia amara, Rotenon, Garde., Ouiikya, Sabadi! . in particular Verabin, fAyanht, in psrAai&r Ryanodine.
  • Viseuro albu (misten, taogwort or common tansy (Tanacekan vuigare), Artemisia absnuhiuo>, hbbea dioie , Symphytum officinale, Tropaonbtin nopus, Quercua mustard flour, Chenopodium anthehrunwicinn, Dryopteris idixmas, bmk of Chinese bittersweet (Cefastrus orbieaiatas ⁇ , Eqnisetani arversse, bark of Ceiasos angalatus, Laminann (Browa Algae), kginic acid (Brown Algae), ChitnCbalnosan
  • Cbenopodium q3 ⁇ 4inoa product kiiowa as HeadsUpo Melaleuca, alfernifolia (produ is known as I anore3 ⁇ 4 CiOkl k yeaanie oil ⁇ , kn wn m Dmgongf1» «CC ) a id flansmi exiraste o naaiaeoel blend of Cdwnopobloro amhookh do.- ⁇ prodneta kn n as aqoiasn).
  • the nrodoets prodneed by mieroorpnisms Ineiirding prmeins or secondary metabolites are selected Iron; bw group consodna. of; i ke ma ⁇ prodneed ay BrvAnks -.mp. s o us proPaets known as !1 ⁇ 2 p->wo k i s w Messenger® , Em l y ⁇ ProAe 5 ⁇ ;.
  • the disclosure relates to a composition
  • a composition comprising one or more bacterial strains disclosed herein and one r snore agrochemicalty active eonipounds, Agroebemlesdv -; ⁇ . ⁇ ⁇ . compounds are substances be are or may be used for treating a 3 ⁇ 4eed 5 a plant, plant part, or ids environment of has sea or plant mekeJIng but not limited to familiagic des, actericides.
  • insecticides aeadcidwa nematleides, mollnsclcides, sabeners, plant growth regulators, piaal mariesus, aheadee; amides ith a known mechanism of action, addinoimi micro rgani s, and biocomrot agems.
  • the disclosure relaxes to a first som position oo orsbng one or more bacterial strains and a seesutd co osition c omp ising ova; or more agroeheubcaliy acnve compounds, wherein ihe fet and second composition may inhibit lain pathogens and/or improve plant peribrmanew Use of ?he first and second compositions may provide a amy erne, additive effects or nue.
  • b-eph provide a 'Onoa aab o eiteci.
  • the first and second aomposittons can be applied at die same base to 3 ⁇ 4 ss - « d, a plant, plant park or the env ironmen of die plant.
  • the first composition cen be applied m fv. seed followed by application oi the second composition so -p.- seed, in yet anoihe* emb diment, die second cornposlpon can be apphed to the seed kblowed by application of dee fhw; composition to the seed.
  • the first composition can be applied to the pla or plant part ifblowed by application of the second composition to pie plant or plant pari, in yet another e bodimcnk the second eompmbrion can be applied to the plant or lain parr followed by appllcsboe of p>e km composition to the awe; or pi.aa park
  • first eonaposstion can be applied to the seed and the second composition applied to the plant or plant p .
  • the second eomposibon can be applied to the seed and the firsi c- -epesife -n applied to the plant or plant pan ,
  • any period of time can elapse between application of da; firsi and second compositions Including bat not limited to 0,25 df a hours, 0.5- 1 hoar, 1 -2 hours. 2-4 hours, 4-6 hours, 6-8 boors. 8- 10 hours, 1 - 12 boinay ay ⁇ - ⁇ hears,
  • compound is an inhibitor of nucaaic acid syntbesia including but no abied to beaai xyb beaaiaxybsVb bapakasata, doyyaeon, dimethinmoK ebaraaob t r&!asyb h mexasob saetaiaxyh am;daxybbl otaracs. oxadixyl sad oN bnie acid.
  • agroehemieally active eonipoand is aa inhibitor of obiox t id/or cell do ,aa; ia.aadap- bui so iaanad to benoaryb ear ) >aad «aias, chbakeaaaole, sahabox op bbaendaaole, p-iiuasamra t iabend zole, ihiopharaae, iiis haaas- «'uyh ? S and soxaoide.
  • raatoabDOSfrobia orysssrrobin. picoxy robin, pyracfeatrobia. arai ra a-. ⁇ ⁇ ' ⁇ . tri doxy troma a ax CHI- raa H ' a i iaakaaa .
  • he a oohetnieally active compoand fta-t b as as an aocoaplar including but i limited to blnapseryL dic!oran, dreobuton, diaoeap. fttt&rio& and atapry!d!nocap, nitroihabisopropyl
  • th& s ommnmlly active compoun is as? inhibitor of ATP production iixdadiag bat not limbed to tenths accaate. ienrin chloride, f ntin hyd xide. and 3 ⁇ 4iUbk»£&n3 ⁇ 4.
  • the agrochs-asicaby active compound is aa inhibitor of amino acid and/or proieia bosynthesis including but not limited to an oprtm, blastiakho-S, evprodinib kasaganry-ein, kasngamyein hydrochioride hydrate.
  • the agmehemkall saiko eompoand is an inhibitor of eqaosreroi bioyymhes s bscluding but aoi naked to glPimorph, axacoasaxoisx bbcatanoi, mioa oaasiok, •. a-;.-a-a: : ad . dickkaxPaaok, dstbnocoaaaok, diakoaaaok, dkrcaaaxok-yb dodoaiorpix aodeaaxah a-cxn,.
  • the agrocbemicaliy aotiva com nd is capable* of iadaaing a best dafeasa inaluding bai not limited to sika aaibaaxoiar-S anoihyi : probonaaoio, mi dadiad,
  • oao embodsai ab d-se agpochamicaby acfiva aosnpoumi is aapabla of maltisae actba iaciadiag bat no booed to bordeaux mixture, captalkb capiaa., chl rothalooii, copper iia hihaaate. copper oaida, eoppar oaayabiorida. aopgar paepanPioas :-aah ⁇ : ⁇ , o er hydroxide, copper sulphat , dichlotiuaidd, dabianosa dodina, dodae baa bass, ; coxa a.
  • peh oPpbaic bnoan, toi jfluamd, zimb at; ?;ram.
  • fungicidal ooarposif a cars comprise una or more agfochemicalty active compounds.
  • fungicidal compositi n comprises ( 1 ) at least one acrsve compound 1 se cted fr m .
  • a leas one setiv3 ⁇ 4- component II selected from the group consisting of: a sir bilunn; an axoka a heterocyclic compound; a ad BCA,
  • the sinabisark is selected from Che group
  • the aa k is sefecieb frof do; group eonsisbtpa of biehkkat:rsaoie :
  • the .heterocyclic c mpound is selected from the group consisting of:
  • the BCA is selected from the group consisting of oearosdapr pyi-beisinc, Uloc!adlwm oudemansi chitosan. Trichoderrtut airoviridcy Ainpelomyces qins uabs, Aspergillus flavuy Aoreobssidnms memelulans, Bacillus subtil ls sanction Candida oteophiia, Candida salt ana, Clonosi&chys rosea f. catenu&ic, Comothynum minitam, Cryphoneeiria psraviiios, Cr tooocc s albidus, Fusarium
  • die agroebemlcaliy active compound is 3 ⁇ 4 bactericide.
  • Bxausples or bactericides include but are not babied to bronopok dkhbrophen. abrspyrin, nickel
  • ibe agroehesrbeaily active compound is n AehE inhibitor including but not bodied to carbaasaies. aiaayearb, aidicarb, bcodiocarb, benfbrac riy buiacarboxbro buioxyearboxiao canvayk carbufbratp earbosabao, edho.kooarb, bdnobaeafb, fonmianate, i raiihocanh, isoprocarb, aiethiocarb, medioaryb metolearb, oxanryl.
  • dboribrrdnphos ohforroephos, ehforpyrifbs (- methyl), coumapho-a cyaoopbos, denssbon-S-- nvathyi, dba iaoo. dicb!orvos/DDVP. diorotophos.
  • the agroehenuca!l active compound is a sodium ebsasoe! modulator or sodium channel blocker including but not limited to pyreihroidsy a g. acrinathrin. aiiethrin (d-ds ⁇ aaus.
  • p-yrebnin (pyrehnnnb Pyrehrine ?e:aaethrin, RU 15525, sPaihioten, tenkahrin, tetramedmin ⁇ ; Re isomers ⁇ !, aaiomerhrin, P:mst1aihnn and ZXi 8901; or DDT or
  • the agaaehenneaUy active compound is a nkotinep e ceey lchoiine receptor agonist including bnt not limited to chloronmotkryk, s.g. aeetamiprid. e!oi knidin, dkropdonnt iaddacsoprid, nitenpyram, Piaeioprid, and nicotine.
  • the hgrochemscdHy active compound is anostanc acetylcholine receptor monda tor inckainyg but not Ite e to spiooyns, eg, s i etomm sod apioove ,
  • the agroehanncaily active compound is a chloride channel acuvaior including bat not Hooted to avenoecrmad y bH-emycins, e.g. absmeePn, emaroec-tin beax te, lepimectin. and aalbemecbm
  • the agrochomicaHy active compound is sjavenik hormone mimic including bra ⁇ ' limited io hydroprene, kinoprenc. meth prcne, tenoxyearb and pynproaytVo.
  • the agroehemie dy active compound is a noo ⁇ spneliie inhibitor including but not limitation to gating agents, eg methyl bromide and ofhar alky! habdey msihyi bromide, ch ropienn. snlkapb fluoride, boarx, and a as a emcks,
  • iln- agrochemicaby active compound is a selective ho iopairoa kaading block r including bat nos banted in p) metro ine and ilonkamid,
  • agroehewicaHy active oemponnd is a able grs>3 ⁇ 4lh infelbltor including bnt not limited to ck>f1 ⁇ 2teainp and etoxaxo!e,
  • the agroehemieaily active compound in one embodio-ink is a nicrobiai disrupter of inset membranes including but not babied to Bacdlus ihiaiogieiods :asb?pecies >sraelensk.
  • th agrotenfc&Hy active compound is m u couple*' of oxidative phosphorykition including bid so limited to eh rtenapyr, sulikimmkk and DNOC,
  • the agfockemictdiy active compound is a nieobnic acetylcholine receptor inckding but not limitation to beosuhap, eartap hyxSroc Joride thiocyelaoc and ihiosuUap-s dium.
  • the agrochemica!ly active compou d is an inhibitor o ebitin biosynthesis including bur no; limited to type id s ch as benzoyiureas, e.y. bistriHuron, obkvilua invm. dbluhenziuxea ikioyciexoron, iloienxoucoo, hexaduoiuroo, iutenaroit, novsknxes, noviilteoi -oa, peni uron.
  • type id s ch as benzoyiureas, e.y. bistriHuron, obkvilua invm. dbluhenziuxea ikioyciexoron, iloienxoucoo, hexaduoiuroo, iutenaroit, novsknxes, noviilteoi -oa, peni ur
  • the agovohcmieally active com und is a inoibtlng disT ote k «:b::idlig3 ⁇ 4 bid not limited to eyroin automat.
  • the agroehernicahy active eompotsnd is an cedys ne receptor agon -i mcluding but e limited to Ji no Imx-uxknes, clxrooMtenoxide, IxboSemxddex mebtcecyienoiide, and tob ft idc-
  • he sgrochemicnily active compound is a ocbepamine receptor agonist clodmg Pot not: limited to anbin .
  • the 8gmehemie3 ⁇ 40y active compound k a mitochondrial complex ill ibeeiron transport inhibitor ine hiding but not limited to hydrameihyitKax aeequlnooyh and itnacoypyrim; a mitochondrial cmnplee I electron transport mhibbor including bat vmt kmked to MET!
  • the agroeheanieaky activ com ound is a rmiars! cxtracr including but not banted to HEAD$?JP#, which is an extract derived bom the seed huh of hcm>
  • da- agroehemlealiy aetrvs compound is a nematichte
  • Examples of •)s:3 ⁇ 4aticldos include but are not lmited to antibiotic nenmtkkks such as a amectin; carbamate nemadcldes such as benomyl, erb; auras e rboayifbi, and cieotbocard; oxirne carbamate neroabektes such as a!arryoarb, akboarb, a!doxy&arb, oxaroyh organophosphorous ne aticides such as diarnbiaibs, iea:aa;p o>.
  • kssthieia pbosphimbdoa, eadssafos, chlorpyrlkos, diciofontbion, dimethoae, ethoprophos, eosuifbda ia i;xsba:sal.e, heterophos, isaabdob>s.
  • i irotos methomyi, h ie, phosphocaby lerbaibs, ihiodicarb, thionaein, triasjophos, an eg ah and mecarphon. Giber cornpooads with neanaucidai activity ingorge acetopro .
  • nem&ticides also include one or more biological nemabeide agents such as
  • the agrochem!ctlly active c m ound can bo a safaner.
  • the agrochemic&J!y acti e ompou d can be a heieroeyOic earhoxyik ; aOd derivanva iackdbeg but not limited to dtehb:sropb ny!pyra?oHn-d ⁇ ca ox Hc said denvanvex. e. .
  • the agrachemiaal!y aeOe compound can be a derivative of Oqainolmol ;a udnu; inn s bnbted to derivatives of parKdkos ⁇ yOxy)acabe cak a.g.
  • the agroehsmioaHy aaiW com und cao be a dOhamxaeetaaPde which are often used as prexinaa3 ⁇ 4anee s;ne « «x (sod active s&kaxorO. inchalna, bat not lauded to Ohchionaid"
  • the axraxhemieafby active compound n be hydioxyaromata: com o n s and aronssnc-aHphabo oarboxyHo a-, ai derivatives a>cii.aiag but a--- ibaited as ethyl 24.5- iriaoetoxyboazoate.4-4> droxy- ,S'Xbaajihv;xy e zoic acal lOObyydroxybeaxoic add Struktur 2,4- d'hydrv-xybanxoia a i .40lyoaad-hydox b£r : e al 2-hydfoxyca!namie ackb 2,4-dchOrodnaaaoe sdd a:;.
  • the agroehemiesHy active cars be a OaxHhydfochmoxaikwZona Incl din bat not lankad to baas-dr F3-(2 » bnenylV lO-xbrpsdrochiaox&bndohioae, bO - .auiaoebx hO-dohxav; - I r 20;hvdn>ahba>xada-2-osso hydro- chOral >o. WO) 2005/112630).
  • Oe ag ⁇ xx:henboaJhy a ex eomaoaad can diphoayimeWosyxeaooe acid derivative including Oa not Haded itjethyl (d! enylmeih xy)a ; i3 ⁇ 4ae (OAS-aleg. No.41858 ⁇ - HW>), ethyl (d?phoi baethoxy ;aoetata or (d henyteasthoxy) .caiio acid (of WO 98/38856).
  • das agrochcaneady cti com ound can b3 ⁇ 4 a jsoihi ⁇ x-hr s3 ⁇ 4anone axlubnm but nor babied to nxeih d O-oxo- ihb 245e a 4bio yraaO/3H ;!dene) nnWh ⁇ oey]aeeiaO (CAS- ag No. 20512.t-04--6 and similar compounds knoaoa from WO 982133 1 ,
  • the agroohamioaiiy aotve coi «po»»d can be compounds tVom the goaap consisting of naphthabe anhydride iaehidiag bat aot banted to (1 acid anhydride), which is kno n s s ed trastmem saieaer fo corn (maise) sgakwt damage by btkwarbamaw herbicides, " teaeionra" ⁇ 5 6 ⁇ dkhiorv2-ph n> pyrimidin « ⁇ .
  • e -.b is ksw n as seed oweewci ssbencr n sown rice agaiast damage by prebkashloo " Uuraeole,” which s known as seed uwaaseat s&ieswr for sorgham against damage by ai chhw and meto!aeh!og "CL 3044 ⁇ 5 !: (CAS-Reg.
  • irony ws.w (2-propenyi iwsx3 ⁇ 4-,3 ⁇ 43 ⁇ 4is iro[4e5)dec3 ⁇ 4r ⁇ s-4 ⁇ 3 ⁇ 4ri dithiosk- ⁇ of Nitrokenia, 'diHsoitbewe ⁇ dadieawi-Sw wthyitbioedtyl pinesphowdhhiossey ⁇ drshokdw (Oy ' RiiethyhO- phen lphosphorothksriey "nwphenate-" (4"Cideny>henyi--n3 ⁇ 4ethyicafbaasate).
  • ⁇ i4) ⁇ : ' -a p. ; a;.ds which besides herbicide! activity also eahiba Sabener activity in crops Hke rice, e.g. "i krocpipceac ' or " ⁇ 93" (S- 1 oredhyk i-phenyiethyi-piperidiadxerbodboatef.
  • Examples of plant groswh regul t rs which may be atemioned are ehlofochohne chloride and odasphon.
  • pi&ns netrients examples include ensiornar inorganic or organic fcnifeers tor stipplying pkmts with macro- and/or rnieronutrieats,
  • the agrocbenkeaily active eotnponnd is a compound with a known mechanism of action. It is to be andersiood that the compounds caa have more than one nesehanism of action.
  • the agroehemicslly active compound ⁇ an be a respiration inhibitor. ( ⁇ ) ifthlMtei*
  • metlryl-aesisnbde acid methyl estcix acid methyl ester, -x-aadix - a isopropyb-a-oxo d-ortho-toiyh? J--di ydfo-pyra3 ⁇ 4aSe ⁇ i-.iirbot:hioi- e acid 3 all- ⁇ s»tor, and 3-nsebvx- ⁇ .
  • active compound oan be an inhibitor of com plex 111 ai Qi site including but not limited to eysxot bd, amisalbrom,
  • the agmohewjeaily s nve compound eaa be an addbaor of complex 11 including bus not limited to earhoxamsdey bsnalaxyb benalaxybM, bonodaaiL. benzo ind i ilupy r, bixafeix b scaiid,.
  • the agroch-smkaUy acti ao-m u d can be a oas imi n inhibitor indduding b wot !iaaited t comple I ncouples,, diflametorirn,
  • ilnom-4--(4--oifuo ⁇ nitropheny! derivatives bmapaaryL dJnobuton, d;noa3 ⁇ 4p, n «a.Kiaany brtnaione; eaaa nomePd compounds: eeatia salts, sueh as fenin-crcePite, ientin hlciade .a lcaaa hydroxide.; and amstoeomiin.
  • bo aproebemicaiiy active ompound can ha a sterol bios nthesis nbibitoi .
  • the agroehernica!b active compound m be a C14 darneth xe inhibitor includng but aot limited to DMi fungicides, t «&»>les: tee naxo , bitertanol remu£ona3 ⁇ 4oie.
  • cyprocon3 ⁇ 4aob diclobatfszob, difeneconazoie, disiconaxote, dinieona ⁇ oie-M, apca3 ⁇ 4ccn3 ⁇ 4a3 ⁇ 4>;e.
  • prothiocoaaao uiacona?oic, simecona ob. tebeconaxob teiraeonaaeb, aavhavabn. triadbaeaoL trUjeoftazwie, anicoaaxoi , i ⁇ [ i?i » S;3 ⁇ - ' .
  • the agrochemb&!l active compound can be a delta 1 -reduaisse inhibitor snolucl ing but not iimted to aldimorph, dodemorph, dodenorpbaeetate, 1 ⁇ 2ipr0pim ⁇ rph. tridemorph, fenpfopidin, pipera! , and spirox& ine.
  • agrochem ahy active compound can be aa inhibitor of 3-ket reducase including bat not limited to fenbexamid
  • the agroenenbad activ compound s a nucleic acid symhe is inhibitor ineiduding but not limited to ( ) phenymrmdes or aeyl amino acid fungicides: bena xyl, benalax l-!Vf, kimi&syl ttt&taiaxy).
  • the aoroeh»micaHy aets * o c m ound is inhlbkor of cell division including bui mi Is mi kid to is) tubulin inhibitors, such as benah y ud x ks or bsiazisoida ⁇ Ie leasin precursor,.
  • oob3 ⁇ 4aib-fsenp ih* agroehemically acive ⁇ a • •nroauri is an inhlbiPr f as'aiao soui r protein synthesis including te& nor Hmiieil to (i) methionine synthesis inhibitors ⁇ aalSiHi-pyrimk!inss ⁇ : eyprodlniL msp&i ' sipyris-ip nd pynrmstha»ib and (it) protein synthesis inhibitors: blaatkadin-S, kasugamycm ⁇ lcaaaporyek; mildiomy tn * streptomycin, o3 ⁇ 4:y;ePacyaiiso po!yoxhte, and vaiidaopy in,
  • tha agroehemieaHy aehve compound is a si nal transduction inhibitor including bu not limited to (s) MAP histidias blna.se inhibitors; iluorolmKl iprodiono,
  • the agfoc ernie&Hy aerjve com und is a lipd or met»brs?58 symbosls inhibitor including but not limited to (!) Phospholipid biosynthesis inhibitors:: edifbiipboP. -pfobanloy rajio b s, !soprothlolaso; id) lipid perovidaPor-: dk ran. nku ⁇ ⁇ . ⁇ . ⁇ no tecna onc, kdciofoo moby . bi iion k eh ronah, and eiridkoaals; (its) phospholipid biosynthesis and cdl wad de os hoi
  • the agrochomieaiiy active compound is an Inhibitor with muhi- ka actiwi inch-sdirsg bid not Isnbted to (i) laorg&oie active ub pinous: Bordeaua saixturo.
  • rgaaoch! rbio c m ounds e.g.. phi sbmsd s, abknod-:-::. ddorotbtrfk-yK ani odrie,
  • nd is a ell wail synthesis in ibitor including but ooi limited to (s) inhibitors of g!aeai synthesis: vaHdaniycrsy po!yoxin B; melann s nthesis inhibitors: pyroqciiosy olcyckyolo, oarprop mld, di yciomsi. and fbnoxand; a carboxamide sel cted ikan the roup cond m rPbr?aacbproaribb. and ⁇ (6 ⁇ siho3 ⁇ 4 ' y q:5>r;dia-b-yi)cyc!opropanacarlx> ⁇ b s k! smidc.
  • the agroch&mcafly active com ound is a plant defense inducer including but not limited to acibonxoias-S-meihyi, prob na ⁇ o!e, isotiadl, tiadsni!, prohexad ne ⁇ akiu ; phosphonatos: io3 ⁇ 4etx !. fosety!-a.hjmin ?T! : phosphorous acid and its salts.
  • the agmehemicaiiy active compound is a compound vvft nknown mode of action including bus not limited o brooopoi, chinomeEhsonat. cyftofemtmid, cymoxaob, dteowei, debae&H di iomoxina, diibnxoquat diienxoqrsat-sTrorb isvdfiits, dipkony!smin, fetip wxmlm, luoriroki.
  • hydfoxybcn coahrH£3 ⁇ 4: b moxynll, dkhkbeebb kxynib (x) sa dazoba nijs: isn canielhsi cax, ka jsasvio , jmaxaptc, imasjapyr.
  • urea* eh roiobiroH, daimuron, dm m, fiuowsskiron, iso rotu p o , lin ron, iweeha enzifiiMwm ⁇ bMthtuw j n ( ⁇ ⁇ ) other acetolactare synthase inhibitors: bispyribac-sodtum, cloraosulam-mefhyL dkdosdasry flotamdam, flucarbazoae, flurastsiflani, etosidara.
  • organo(thio)phosphates acephate, azamethlphos, axiaphos-methyk cblorpyrifos, chkayjyrifos-me!hyl, ch rfbnvinph s, diaxinon, dich!orvos, dicroKsphos, dimeth ate, disidfototy eild n, fealirot ijn» t3 ⁇ 4fithio;y iodometbane, isoxathiosy ma!adik> « : methaTnido hm, metbidathion, methyl- amdPoix mevjnphos, momjeroto bos, oxydsioeton-aieibyb paosoxoo,
  • phosrneb phospbarnidor pborase, phoxkny piriaopbos-nseUyvb proffenoibs, prolhioiby pyrikipikimsoa, sulprophos, teirachlofvmpks. ⁇ «rbui3 ⁇ 4s, Mra ⁇ etb il tbHn, triazopbos * mck rfon, ⁇ carbamates:
  • akaryogrb aidicarb,. fcandiocarb, beafeaearf eatbaryk ca ofdrasy carboadkay fenoxyearb, fi.;ratbi cafb, nwthiocsfb. rnetbomyk oxa yi. plrkrkearb, pmpoxur, h dicafb, iiiaas aie; (ii) pyrtthroids: a!leih n, bitenthrin, eyf!uthrin,, cyhalothrss).
  • Uqcouptets ekiorfenapyr;
  • LCOs ere signaling c mpounds dud Induce f s oariy stages of noduisiion in piani ro ts, which load t the formati n of root nodnks comsirdng the oimmenA iog rhimblal bacteria.
  • Foliar application of L Os has also beet ; demonstrated to increase pisoios -a ⁇ hesis. sod fulhng aod to e iog in crop and horticultural plant species.
  • LCOs consist of ao oligomene backbone of j L -linked bi- cciy -D-gioeosandae ( ⁇ Glc Ac") «?sldnes wgg an Ndhsked fatty acyl chain at the nonredncing end, LCOs differ in the number of Gko Ae resodues in the backbone, n the length and degree of saturaton of live ssfty ac i chain, and in the sabstitotions of reducing and oonredncing sugar residues, ! .Cf) structure is characteristic tor each rhiKobiaJ spees.es. and each strain may produce m iisple LCO's with different structures, LCOs are the primary deteoionants of host specificity in legume sy biosis,
  • the disclosure relates to a composition comprising a bacterial strain disclosed herein and one or more LCOs.
  • the compostOn can to fiber comprise one or snore agroehernicahy active compounds.
  • the composition can bother comprise one or more BCAs,
  • die LCO includes bet is no? limbed to the iLllosvin str ctures:
  • composition c3 ⁇ 4 « comprise one or LCDs includin ' but not limited to the f llowing:
  • Composition LCO 2 (above?
  • the bacterial strains disclosed herein can be us d with ne or more
  • I oflavones inehidiog hut not limited to gerdsiein, biochaain A 10, dawfeein, giyed in, hei eiodn.
  • the iscl sure relates to eomposi ons compridng one or m rs baderiai strains disclosed herieio and quin xoii «eo
  • the oornpositioa c r; fdrther comprise o;e or more agroehemscaily aeaoe compouads rid one or more BCAs.
  • the composition comprises a rmxiis e, comprising as acuv compounds () ⁇ at least one compound of formula I
  • Ri is W or F; R:2 k H or C!-13; R3 is H or CH3: M is CR haiogeru C2U22-a!kyi or CI Coo a!koxy; n indicates e number of subshucn ⁇ R4 on ihe phenyl ring and n s 0, 1 or 2: Fy is pyriirudyi or pyndyL wherein the aforernen boned heieroaroroaiic radicals are ia;3 ⁇ 4 «bs;haied or carry- h 2 or 3 sden crd o dveram snbyihoeats Ra; Ra is CN, halogen, Ch-C2- ikyb C!
  • ⁇ UP ⁇ ' -;.- v :-V I V ⁇ " ⁇ -. ⁇ h : : l H I ', ⁇ ⁇ ' ⁇ . ⁇ .' ⁇ ⁇ :>'. pHi.': p; ⁇ H ⁇ ; ; ;- foiVvl - 1 ! f-j 1 P. U ' k. P-.; .
  • 8 is a direct bond or n idk iene goaup; A k OH or H; and a HP : ⁇ .
  • ⁇ n another erobodknerp compo nd I is selected from the gro p vonspdng of($,8- ⁇ !ifH*om- q ⁇ u ⁇ s3 ⁇ 4oknp- k-- d p --trph.!or msd-pd-pyrp
  • the composition can bather •:oaa ⁇ ;- ⁇ -? one or nnare sgroehemicady active compounds and one or more BCA-;.
  • the bsckrisl strains dke sod herein can be used ssmukanoon-siy, ssquem f with a composition comprising the compound of formnla !IIA. in another ond>odimeop t & bacterial strains can be within the satse composition as the msociicidai compound of formula if in ore; embo ment, the disclosure relates to the use of th - bacterial strains disclosed heoon with a conrpos'Uoa comprising azo pyrmndinyiamine of t nmrjit HI.
  • the cooiposshon can Rather comprise one or more agroehernicalry active compounds and one or more BCAs.
  • the composition comprises a fungicidal nbxa -c com risn , as active components: 1) a axoIopyrmOdbydamine of the formula lib
  • R ? is Cb-Cc alk i, C ; rCR alkenyk C Ci a koy alk C r C,- ycioaikyk phenyl or pheivybCY Cb-atkyi: R 3 is C r e mlkyl C : 1 ⁇ 2 ⁇ ikim i, C r C.
  • Ra is halogen, oyamy hydroxy!, mercapRy C : nyy 3 .-atkyk CrCto-ha!oaskyi, C;-Q-cycio3 ⁇ 4!kyL C; C ie tdken h CR C : - ikyayi, Cb-Cy-alkoxy, CrQ-aikykhio.
  • lb is hydrogen, halogen, cyano, N!RlkR hydroxy!, mercapto, C r C ⁇ .-a!kyl, C r C s ⁇ ha!o-slkyk C C;rcycfca!kyi, C r C 4 -aik.
  • the corn position further comprises a compound selected from the group consisting ofc 6-Ry4--dichka3 ⁇ 4 ⁇ -od
  • the compound of the formula JJII and the agrochemcia!l active om ound are in a weight ratio of fwm I 00: 1 to !: ⁇ 00. ' . € ⁇ 3 «»pm i&M om rim a fwtyeierie, teals mm anM
  • the disclosure relates to the use of the bacterial strains disclosed herein whh a m o&idoa composing a polymeric compound., and ionic cotfipoutid or a eoroponmi with imidaaohnm groups.
  • the composition m fmiter comprise one or m re 3 ⁇ 4gr chemlcally active compounds and mv o? mere BCAs,
  • t e com osition comprises at least one polymeric c m und, ionic compound comprising ⁇ mid olams groups (imsdax-oiiuwi compound), whic consists; essentially of at least 8 repeat unts of the general
  • R ; and K ' are uxlependently hydrogen and in each ease unsubsiinned or substituted CrCar lkyf C Cjy-a!koxy, C C 3 o--a!kyl!fno.
  • aliphatk-abcyosic, aromatic or araRphatk radical where the aliphatic moieties in the aforementone aliphatic, aliphatte-alkyche or araRphatk radicals may be internipted by OHO or m e nonadjaeent groups wMeh are selected from --0--, •-S-- arsd ⁇ (R t: ⁇ --, where R !' is selected from hydrogen, C C ( ralk i and a group CHy -R-R) Y -Rc. wherein y is R 2, 3, 4, S or 6 and ! is hydrogen or C C ; -akyl: whe?
  • aheycisc or aromatic moieties in dre aiorsaaerasoned alicyclic, aHphHtk-alkyeik, aroatatk or araiiphaiic radicals may be substituted by R 2, 5 or 4 radicals selected from sk-d h w t r ..-abow a radical of die formula -() llyCHyOj ⁇ -lR 1 , where * 5 is hydrogen or C r r-alkyi and a: k R 2, 3, 4, 5 or 6: earboxyi and carboxylase, and where the ediphaiic moieties in iho aforementioned aliphatic, aliphati -aikyclk or arahph&tic radicals may bo sabstlttjiyd by R 2, 3 or 4 radicals selected from C r € ;; -a]koXy, a radical of t e formula -O
  • R ft R hydrogen or C r RValkyl and ⁇ is I, 2, 3, 4, 5 or 6, earbosyi and carboxyiate: with the proviso thai the arernavk or nraliphatic radical-? do oot contain 1 ,4 -bound phenvlene rings; and does not contain 1 >4-boumi oheny!enc units and is not 2-hydmxy- propane- 1 ,,3-diyl or 2- ydroxy-2-melhvJ- propane- i3-di l; and where one C!
  • compositions comprising safes titated thiopbeoes
  • the disclosure relates to the use of the bacterial strains disclosed herein with a composition comprising a substituted thk>ph «n ⁇ s.
  • the c mpositio can further comprise one or moe isgroc!s micalK' active compounds and ⁇ >> ⁇ or >>.; ⁇ ⁇ - ⁇ BCAs.
  • composition omprises substituted thiopheics of formula V wherein: k indicates the nunrber of the oxygon atoms bound to the sulfur atorn of the thiopbene moiety and k Is 0, I or 3: R is C - ⁇ OX>R c or -CH ⁇ -G-R £ ; X is O or S; R i: is hydrogen, CoCd-alkyl C2-CC aikenyL C2 d4-alkynyh -0 ⁇ R K or C 1 ⁇ ' / " indepeodent!y of one an iher arc hydrogen or CoCCalfcyk IC ' is hydrogen or Ci ' « C4-alkyi; provided that s!i four R substituents are kiemioah
  • the disclosure relates to the use of the bacterial strains disclosed herein with a composition comprising a substituted diih mes;.
  • the composition c n further comprise one or more agrocherajca!ly active compounds md one or more BCAs.
  • the composition comprises cobstlmted dirbdine of !bn ds VI:
  • k indicates the mimber of die oxygen atoms bound to orre sulfu atons of the dubbne moleiy and k is 0 or I : all four R std-sCmenis being kle ieag and R is C .
  • the disclosure relates t the use of the bacterial t ins disclosed herein with a composition comprising fiuophyfamm. Bacillus and a SCA.
  • the composition can further comprise one or more agfoehoodeaily active comp unds.
  • th ⁇ composition comprises: id ) Fluopyrasn, (2) a spore- forming bacterium of the genera B iiias, snd at least cm? BCA.
  • the spore-dorm ing: bacterium is selected from the group consisting vfMa iiim strain CNCM C i g y, B&dfius cere . Bacillus omodo Bacillus
  • the BCA is selected Worn the group consisting of: bacterid fungi or yeo , rotos aS ; viruses. «niomopaiho ⁇ nie nematodes, inoeidants, botanicals, and Harpin f produced by Erwinia amylovora).
  • the spore-d rndng bacterium of the genera Bacillus and the BCA are not identical.
  • disclosure reate to ihe us of he bacterial sin-uns disclosed herein with a composition comprising one or more emirninoearboByi compoun s.
  • the composition can further comprise one or more 3 ⁇ 4grochemio3 ⁇ 4l!y active compounds r ' BCAs-
  • R is m thyl, cyelopropyi or 2,2--d!uioroethy
  • The- composition ecm fimher comprise one or more ags'oohemicaiiy active compounds or BCAs, in one embodiment, the composition comprises a i;0 ' s idtooiig ⁇ :S3 ⁇ 4eeh;rri;ie of ' Sbniinla ( VI)
  • C represents a soNbmeP chosen trom -0-, o? ⁇ . R3H2-, -CHR; 7-, ogRp Rig.- and -bRcRh
  • D reps'esentx a linear or branched, saturated or uns t rated hydrocarbon-based chairs containing irons 2 to 2b carbon atoms;
  • Rl represents a stsbstituent chosen Rom H. CbR-xdkyb QO)H and C(0)CH3; R2, R3, RR RI4, 15, Rib and R 19 represetn, indepeodemR of each other, a s-tbstRnerst chosen roan ! ⁇ !, C i -bodkyk bo PCI a ,uk;2. -CfS I diodkyb Xs R d --bodbyh ⁇ ⁇ : -C(S;NH2, RdRR)NH2, ' tORRRb bodkyk R:S. Rd o-eikm and -C(N f H) HCS Xadkyk
  • R4 represents a snbsbtnent chosen from H, CRRodkyi and R2! ;
  • R represents a snbsbtaent chosen trosn t Ci Rtcosyi and R22:
  • R7 represenis a suhsthuen chosen from bb CRRalkyb arabioosyj and R23,
  • R.b represents a sybsthuent chosen fr m U.
  • CRboilkyb taeosyb nuahyth-ioosyk suitbRtcnsyh acetyifucosyL arabinosyh S03H. S03U, $03Na.
  • RR represents a suosbsaent chosen fro i ti. CRbo kvf mannose. glycerol and R2R
  • R j I , R i 7 sn-j R 1 b represent, indessvndonby of each other, a sh iduen; cbnen ⁇ ' ⁇ so C 1 -R- sikyl and R;
  • R2b R 1. R22, k sa R24 and E2S onscreen independeat!y of each other, a snbsbtnent chosen from CXO i R-obkyi, -C(S ⁇ CI ⁇ odkxb Ok-s ' X; -6-aibyb ⁇ : ⁇ ) ⁇ 2.
  • the disclosure relates to the use of the bacterial strains disclosed herein with a composition c mp is ng 3 ⁇ 4
  • the composition can comprise one or more agroche ieaiiy a ttve comp unds or BCAs.
  • the a dithiiisooen acfa'bosamsde baa formula VH:
  • cal and represent methyl, ethyl, n-propyi or isopropyi and a represents 0 or 1 , or an agrochemaeally acceptable salt thereof,
  • the composition further com rises a BCA selected from the group consisting of ( 1 ) bacteria; (2d fun i, yeasts; and (:>) IsoOavones.
  • a composition disclosed berena may comprise n or more additional com onents ⁇ - Preftrabiy, any additional componen is) do nut materially attecr tire anti- ccaiiammant properties of the composition of the present mventio?;.
  • die additional componentis may be a carrier, an adjavant, a so!ubiliiiog agent, a suspending agerd, a diluent, an oxygen scavenger, an antioxidant a food materia!, an anh- eonmminaot ent, or combinations thereof
  • the additional co ponent is) (n y be required lor the application m which the strain or com os tion is to be utilized.
  • the additional ccnn>oagnt(s ⁇ amy be an agriculturally acceptable carrier, escip ent or luent.
  • the additional component ⁇ may be an edible carrier, exdpient or dbuenb
  • the one or more additional c mponents is a carrier, excipient, or dibsem.
  • Hxarnples of nutritionally acceptable carriers include, for example, water, salt sohaioas, alcohol silicone, waxes, peuoleam jelly, vegetable oils, polyethylene glycols, propylene glycol, liposomes, sugars, gelatin, lactose, am lase, magnesium stearate, talc, surfactants, silicic acid, viscous paraffin, perllana oil, fatt ae!d moti myee ides and ag leee-ndes etfoetb?*! fatty acid enters, hydto ymeihyb cellulose,. pc yvhylpsaToPdooo, and die like.
  • Examples of exeipieots kiolade ut re no limited to: fwjerocrystalhne cellulose and other ce!loloses, lactose, sodi m citrate, calcium carbonate, dibasic calcium phosphate, glycine, starch, milk uucu. and high molecular weight polyethylene glycols.
  • the other c mp ent may be used simultaneously (e g. w n they are in admixture together or even when they are delivered by different routes) or secyentially (e.g. they may be delivered by different routes).
  • the c mp siti n er its adeem may lso contain chela i g agents such as ED ' fA, eibac ac d, tartaric acu!, etc.
  • the composition or hs diluent may contain aepve agems selected fm laity acids esters., such as mono-ami digdyeeiides, non-iomc suriaci&ms, sucb as pdysorbates, phospholipids, etc. Bmulslfiers way enhance (he stability of the composition, especially after dilution.
  • the bacterial strain aad/or compositions ma !> « used in an suitable lorn? - vheheer ⁇ ' ⁇ hea alone or when present in a composition.
  • the beeleriai s ream: and/or cougsoshtons may be formulated la any suitable way to ensure dost the composition cornps ises an acm e compound: s ' i oH er-ess
  • t he bacterial strains and/or compositions thereof may be in the fonts ol a dry powder thai c n be sprmkled on or mixed in wkh a product.
  • the bacterial strains and/or compositions lis bu: form of a dry owder may include aa additive such as microcrystalline cellulose, guns rragacanth, gelatin, starch, lactose, alginlc acid, Primoge!, or corn starch (which can be nsed as a dislntegmbag agent),
  • bacnsnal strains and/or co pos tions disclosed herein can be a spray , dried iermemate re-suspended m HjO to a percentage selected from the following: .0S-- 1 , 1 - x syf 5 - a 7- 10, 10- 15, 15-20, and greater than 20%, in smother embodiment, one or more than one clarification step(s) can be performed prior m spray- drying,
  • the compositions disclosed herein can comprise a suspeobn spores from the bacterial strains disksed herein.
  • the suspenesion of spores can be in die range of x l ' - m 1 A I O CFU/mi.
  • the oornpostions disclosed hcre-e can eoujpnse eoneenirared, era J spores from the bacteria! strains disicsed bereiri.
  • the concentrated, dries spores can be in the range of ! x 10 s to 1 x l:0 n CFU/g.
  • bacterial strains and/or compositions disclosed herein can be appbod in wet or partially or completely desiccated form or a stony, gel or other form.
  • bacterial siraias askbor compositions can be rai sed with a carrier.
  • the carrier ineh.rdes bat is no babied to whey, ntalP>dexirin.
  • the strai s can also be used obth o with u preservatives and in concentrated, erreo ⁇ Ksernrated, or diluted form, I» om.
  • the strains can be in the form of a pellet or a bioiogicibi pure pellet.
  • the sirains described herein can be added to one or more carrier.
  • the «atwr ⁇ s> and the strains can be added to a ribbon or paddle mixer and mix d for about S mirurtes. altboagh die timing cars be inoneased or decreased.
  • the components ar blen ed - w. h that a unif m mixture f the euhmw and carriers.) is produced.
  • the final product is preferably a dry, fivwabie powder.
  • the bacterial strains sn -r eomposibons may be ibrrmdaied as a liquid, a dry powde , or a granule.
  • the dry powder or granules may be prepared by means known to those skilled in die arb >;o b as. in iop-opray fluid bed co&rer, a; a bottom spray ofs er. or' by drum -a-annao- -a o .p. high sheer granulation), extrusion, pan coating or n a mlereongfedlents mixer.
  • bacterial sirams and/or compositions may be provided as a spray-dried or iree3 ⁇ 4wbned powder.
  • the bacteria! saralns nrmd r compositions arc in a liquid ioroHdaboa.
  • a liquid eonsumpllon may conPbn one or more of the following: a buffer, salt, sorbitol and/or glycerol.
  • the bacteria! strains and/o compositions disclosed herein may be fonmdsted with at least one pirysadogicaHs acceptable carrier selected irons at: least one of inaiiodextrin, limestone (ealcmm carbonate), cyciodestnn, wheat o a wheat component, sucrose, smrch, N j Cp, Talc, FVA, sorbitol, ben oate, sorb costume, giyeero!, sucrose, propylene glycol, 1 ,3 -propane diol, glucose, parubens, sodium chloride, citrate,, acetate, phosphate, calcium, metablsulfite, formate and mixt res thereof
  • the bacterial strains, and/or eoinposfioris can include a seed, a part of a s ed, a plant, or a plant pan.
  • All plan s, plant parts, seeds or soil can be treated in accordance with the bacterial strains, ompositi ns ami methods; disclosed herein, ibe compost sons disclosed herein cat! include a plant , a plant pari, a seed, a seed part, or soil
  • the bacterial strains, compositions, and methods disclosed herein can be appl ied to the seed, the plant or plant parts, die fruit, or the soil in which the plants grow.
  • the plants and their parts may be treated with the described compositions by applying the compositions directly to She plants or plant parts.
  • the plant and plant parts may be. treated indirectly, tea y t eating the e «vin»am#M habitat in sehleh e plant parts sm or are exposed to.
  • Conventional treaimetit methods may be ased to tea the environment or habitat Iming dipping, spo aog. migating, fbggsng, rao nay. brushing, ore iaieefiog.. and, in the case of propagation materia:!, m particular seeds, f thermors by coating with one r mo e eoaas,
  • the disclosure provides a method of increasing plant performance by applying one or more bacterial strains or co ositions dis losed herein to soil seed, plant, aod/or nbad pnos in ⁇ . ⁇ > ⁇ >. ⁇ . ⁇ embodime t, the discl sure provides a method of isKa3 ⁇ 4ebng plant perba-mance by applying one or .m-sv bsaaerial strains or compositions disclosed herein to sod seed, piem and/or plani parts in ass effective usinea :o ineiease plans performance.
  • die disetosore provides a method of increasing plant heakh b appl ing one or more bacterial strains or compositions disclosed herein no soil, seed, piano and/or plant pasts.
  • the disclosure relates to a composition so improve plant perlbrmanee comprising at leas one bacterial strain selected from the group eosssistnsg of fttebvbi* ; :a eobffopbiea?a.p aciihi iichmifhn , B cui s biins. nd Famib ciii s poiymyxa in an efreebve amccta: to improve plant pesalasaaa.ee.
  • the disclosure provides a method of ines-easing crop or plant yaekb
  • the disclosure provides a method of increaarng planr weight or si3 ⁇ 4e sedative to a:a untrsateci pistil.
  • the disclosure p ovider a method of increasing crop or plant yield emnpns g spplymg at least one bacterial strain or composition described herein to sod or a seed In a ilrsi appiicasbu followed by applying at least one bacteria! strain or composition described herein in a folar application.
  • a bacterial ssmiu or eonip stiion described herein is applied to soil
  • a bo application step, applied a:; seed in a second appnesaior>.
  • au-J - applied to die lobar region of a plant
  • the bacterial strain or composition in he I st second and third applications can be the same or different strains or compositions,
  • the disclosure provide* s method improving plant perforosatree comprising: (&) spnlylng a first bacterial strain or composition to a seed: (bj applying a seeond bacterial strain os eomposioon to soil surrounding the plant produced bssm the seed; and (e) a l in a barb haetsriaj strain or eomposkbn to an aerial plant par of the plana .
  • the baessrub strain or eomposibon n vis* o o --. w -no and th rd appl ications can be tbe same or different strains or compositions,
  • ennbodirrieiig tbe disclosure provides a method tor improving f>k pettbrn-nmee comprising: (a ) apply ing, a first bav eriai strain or eomposh bn to soil; (00 applying a second bacterial strain or compvsition to a seed: nd (c) applying third bacte ial strain or composition to an aerial plant part of a pfcat produced tknn the seed planted In tbe soil.
  • the haeierbd Sp in or composition In the first second, and third applications can be tbe same or dit etem sussbns or eomposibons.
  • the disobsure provides tor a method tor improving plant porformanee comprising: (a) applying a bacterial strain or composition described herein to an aerial plant pad: of the plant.
  • the disclosure provides for improving plant performance comprising applying a bacterial strain or composition described herein to the foliar region of a piano
  • the disclosure provides lor increasing crop y!dd comprising: (a) applying a hbsst bacterial strant or composition to a seed; (b) applying a seeond bavferiai strain or composition, to soil surrounding the plant produced bo m the seed; and (c) applying 3 ⁇ 4 third bacterial strain e conmosibon to an aerial plant part of the plant,
  • the disclosure provides for method of increasing orop y ield comprising: t a t applying a first bavterral strain or composition to sob ; (b) applying a second bacterial stab or composition So a seed: and prO applymg a t rd bacPenal strain or composition to an aerial plant pan of ss plant produced i f ⁇ : ⁇ ⁇ ⁇ . ⁇ the seed planted 1st the soil.
  • the disclosure provides a method of leoreasing crop yield comprising: applying a bactenai strain or composi ' doo described herein to an aerial plant past of dve plant
  • the disclosure provides a method of increasing crop yield comprising: applying a bacterial strain or composition described herein to the lobar region of a plant.
  • the bacterial strains, comp si ions, and methods described herein increase crop yield by about 10% to about 20%, a out 10% to about 30 .. about 10% to about 40%, about 1 % ⁇ . t about 90%, about 20% to about 80%, about 30% to about 70%, about 40% to about 60%, or about 5% or more, about 1 0% or more, about 20% or more, about 30% or more, about 40% r more, about 50% or more, about 60% or more, about ?0% or rnnre, aboot 80% or more, or abotit 00% or more compared to an untreated seed, plant lant part, crop, fruit, or vegetable.
  • the bacterial s ains, compositions, and method described herein increase crop yield by about 5%, about 10%. about 20%. about 30%. about 40%, about 50%, about 60%, about 70%, about 80%. or aboot 90% compared to an untreated seed, lants plant part, crop, trad, or vegetable.
  • the bacterial strains, compositions, od method described herein i crease lan vg r by about 5%, about %%, about 20%, about 30%, about 40%, about 30%. %>oet 00%, about 70%. %o>u b0% relief or ab ut 90% compared ro an ontrea;3 ⁇ 4d seed, plant pl in ;3 ⁇ 4.: ⁇ crop, bant, or vegetable.
  • abour 40% to about 00%, or abota 5% or more, about 10% or mcrre, aboou 20% or more, about 30% or more, about 40% or more, about 50% or more, abool 60% or m re, about 70% or more, about rP0% or more, or about 90% or : ⁇ .:- ⁇ / ⁇ compared to at) entreated seed, plant, plant part, crop, fruit, or vegeabje.
  • dse %cas up strains, compositions, assd fnothod described heroin increase plant weight anddsr sloe by about S%, about 10%,, about 20%, about 30%, about 40%, ab ut 50%, about 00%, about 70%, about 80%, or about 00% compared to an untreated seed, plan, plant past, crop, fruit, or vegetable.
  • Phosphorus is one o; 17 a,ancra. ⁇ essemittl tor plant growth, bp, functions cannot be srlbrmed by aay other nutrient, and m adequate supply of P is required for o timum growth and reproduction.
  • Phosphorus (P) r vtial io plant growh a d is ibuud in every heme plan! ceil it «s volve-d in eovr 0 key plant functions, ncludin ener y txansf r, photosyot es . tmnsfonr rtion of sugars and starches, nutrient movement within the plant, and transfer of genetic characteristics frost one generation to the next.
  • Phosphorus is classfed as a major otPnent meanin t its ft3 ⁇ 4qus «tiy deficient for cmp ptxxluetfon am! is rep;.;li-ed by plants rebrbvoly larg « amounts, the total 0 eommnhauion in agricultural plants generally varies f% 0.1 to 0.5 percent.
  • Phosphorus plays a vital rede in virtually every plant process that involves energy transfer. Bigh- «n»rgy ph s hate, held as a p rt of the ehemleal structures of ad n sine diphosphate (ADP) and ATP, is the source of energy that drives the multitude of chemical reactions within the plant. Who?; AD? 5 d AIT transfer the high-energy phosphate to other moieetdes (termed phosphorylation),, the stage Is set tor many essential processes to occur. !3 ⁇ 4:ssphorous k also a component of phvtaa a major storage toon of P in seeds, About 50 percent of the t ps!
  • the disclosure mkies to bacterial strains, compositions thereof, and methods thai can solimiilze phosphate.
  • the disclosure relates to bacteria* strains and compositions thereof that can soluhllise phosphate in «.oil. i yes smother em d ment, the disclosure relates to bacterial strains and com ositions thereo dta; can soiubilix phosphate and reduce h need for phosphate !ertibaer.
  • the disclosure relates to bacterial strains and compositions thereof that cars increase the amount diphos hate availa e to a seed, plant, or plant part.
  • the bacterial strains an compositions dis l ed herein can increase the total P concentration in plants.
  • one en ⁇ o ime t th total p concentration in plants is increased by percentage selected from the group consisting of from ) % to 3%, mam 3% to 53 ⁇ 4, from $3 ⁇ 4 to 7%, from 7% vo 9%, from 9% to ! I %, from 1 1 % 13%, from 13% to 1 so from 1 5% io ! 7%, from ! 7% to 19%, from 19% to 25%, and greater than 25% as compared io an untreated control.
  • the acterid stoma; and compositions- disclosed herem can Increase the total ⁇ concentration in phytin.
  • the total F concentration in phytm Is Increased by a percentage selected torn the group consisting of mam 1% tj a%, fi a 3% to 5%, from 5% to 7%, fern 7% to 9%, fr m 9% io 1 1%, from i t% to 13% : from 13% to 1 5%, front 15% to ! ?%, from 17% to ⁇ 9%, from 19% to 2%o. and g; ⁇ ;.. ⁇ . ;er than 25% as compared to n untreated control.
  • the disclosure relates so methods to increase phosphate.
  • a method for increasing phosphate comprises applying at least one bacterial strain disclosed herein to a seed, a plant, or plant part
  • a method for increasing phosphate comprises applying a composition comprising least one bacterial strain disclosed hernia to a seed, a plant or plant part, hi still am her embodiment, the method comprises applying the bacterial strain or composition to a seed, a plant or plant part a; soli- In another embodiment the composition further compmes one or more agrochemic&lly active compounds.
  • the bacterial strains for modulating phosphorous are selected from the group i ifisistln or: B i ' ius a&yloi;qNify ie arid Backi //c/aeir/ormfr.
  • the naaterlal strains tor morhtkrtmg phosphorous are selected from the group consisting ot: Baailm saay ten sma.: a ess BS27 BiicUi yloik dfa le BS2084, Badilw r>n7oHquiiacie ! 5AP : Bact//;s awyhli. ifaciem 5. ⁇ P f Bacillus cunyloHquifttciens tSSAO i . Bacillus ot hHq hci s ABP2 /8, Bacillus
  • phos horous are selectee rAsm the group eonsisgbig oi: BAS42, aciH s k-hemfhmns BL2 1 , and combinations thereof
  • PhoR-P transfers the phosphate to the transcription iacior pnoP, forming phosphoryiatod phop.
  • Phosphoryla ed phoF activates expression of about thirty different genes, mc hiding a!ka! e phosphatase, by binding io the pho boxes located upstream of the phosphaie-aegakrted genes.
  • the disclosure relates Pa bacteria! spasms nd compositions thereof thai contain the phoP gene, in s-s ahes eamodlment, the disclosure relates to bacterial strains and
  • compositions thereof that contain the phoP ger-e and aid its the breakdown of phosphate relate to bacterial strains end compositions thereof that contain the phof gene and aid la the breakdown of phosphate, thereby reducing the need tor phosphate iertibaer.
  • the disclosure relate* to bacteria! strains and coaspoaitions tbereof that contain die phoP gene and ep. n veb breakdown phosphate It) the sod,
  • a method to atd in the breakdown of phosphate comprises applying at least one bacteria] strain comprising the phoP gene to a seed, a plant or plant pari in another mbodi ent a method to aid Itt the breakdown of phosphate comprises apphAng a composition comprising at least one bacterial strain comprising the phoP gene to a seed, a plant or plant part, it; still another embodiment, the method comprises applying tin: bacteria! strain or composition to a seed, a plant or plant part in soil, in another embodiment the eomposltloa bather comprises one or more a onomical iy active cotnpearKls,
  • a atethod to increase production of aPmbne phos hat se comprises appK Age at least one bacterial strait; mprising the phoP gene to a seed, a plant or plant part, in another embodiment, a atethod to increase production of alkaline phosphatase comprises applying a composition comprising at least one bacterial stram comprising - he phoP gene to a seed, a plant or plant patt.
  • the te mod comprises applying lite bacteria! strain or composition to a seed, a plant or plant part in soil, in another embodiment, the composition further comprises one or more agrochemleally active compounds.
  • the bacterial strains containing the phoP gene arc ft . ; . ⁇ oo AAmnmaaeaS . in ot;e embodiment, the bacterial strains oonraln g the phoP gene are selected Irons the group c nsisti g of: B i!iu* itmyktHq iack B3 ⁇ 4 r, iteei&s xmyhiiqui/ ck mM4, it «cid3 ⁇ 4o
  • each cell Is capable of producing hormones
  • Plant hormones shape the plant, effecting seed growth, time of flowering, the sex of flowers, senescence of leaves, and fruits, They affect which tissues; grow upward and which row downward, leaf formation and ste growth, fmh development and ripening, plant longevity. ; : ⁇ :0 evers. plant death.
  • H rm nes are vdal to pk t growth, and. lacking them, plants woud be m sih a nws of rmdlfi enviated eeds.
  • the disclos re relates to bacterial strains,, and compositions thereof thai increase the production of plant growth hormones.
  • the disclosure relates to Pa, on ml strmray composidons thereof, and methods that contain genes thai modulate the product ion of plant growrh hormones.
  • the discl sure relates to bacteria! -mo s and compositions there f* hat contain genes Ueu increase the production of plant growth hormones,
  • Auxi are compounds that positively Ini!eetwe cell enlargement, bud ibm tioty and root initiation. They also promote the production of other hormones and In cosywrsotion with cytoki ns, they control the growth of stems, roots, and fruits, and convert stems into flowers,- Anxies affect cell elongation by altering eel! wall plasticity. They smrnn!ate ca bi m, a snirrype of menstem cells, to divide .nr.! in stems eanse sec ndar xylem to dnAaemrm:
  • Auxins act to inhibit the growth of bods lower dene the stems f spies! dominance) and also to promote lateral mi adventitious root development and growth.
  • Leaf abscission is Initiated by the growing point of a plant ceasing io produce a cams Auxins ' ⁇ ⁇ ⁇ seeds regelate specific protein synthesis, as they develop within the flower after pollination,, causing the flower to develop a fruit to contain the developing seeds.
  • the moat common auxin found if; plants Is iodoie-S-aoetic acid or !AA,
  • the disclosure relates to bacterial strains and compositions thereof that nx rease the production of auxins.
  • the dkclostste rekka to bacterial sereins, eemjwWoiw thereof end methoek that contain genes that modulate the production of a>r ms.
  • the disebsnre eei ates io bacterial strains, and compositions thereof that contain gen «s thai increase the production of auxins.
  • the disebsnre relates to bacteria! strains thai contain the 2,3 - butanedio ⁇ deh drogenase gene (bdhA) and c m s ons ibaorof that modulate the ro uction of auxins
  • the disclosu e relates to bacterial strains that contain the bdhA gette, and com ositions thereof that increase the production of auxms.
  • the bdhA gone is associated ⁇ . ⁇ ah production of , bntandiol a volatile compound associated with pJan* aux in prodoe on and regulation.
  • the disclosu e rs? cs to bacterial strains that modulate the production of 2,3 btnandlo! cab compositions thereof
  • the diseiosrjfe relates to bacterial strains that modulate the production of 2, butandioi and compositions thereof is?
  • another embodim nt, the bacteria! strains, compositions, snd methods disclosed hete can increase the am u of auxin in plants, in o-ne embodiment, au i in ptertts is increased by a percentage selected from the group consisting of horn 1 3 ⁇ 4 to 3%, from 3% to 53k from 5% to 7%.
  • the bacterial strains, coaipesiilomp and methods disclosed herein can increase expression of the bdhA gene to plants.
  • a percentage selector! from the group consisting of from 1% io 3%., from 3% to 3%, l3 ⁇ 4orn 573 t 7%, from 77;, to 9%>.
  • the bacterial strains, compositions, and methods disclosed herein ears increase 2,3 b tarsdtoi in plants.
  • the dkelosnte relates te methods to Increase production o plant ations, in one embodirnenf a method to increase pi-oductioo of plant attains comprises applying at least one bacterial strain co rising the bdhA gate to a seed, a plant, or plant pari, in another embodiment a method to increase production of plant aux ns contprises applying a composition comprising at least one bacterial strain comprising the bdhA gene to a seed, a plant or plant part, in still another embodiment the method comprises applying the bacterial strain or composition to a seed, a pla or plant part in sod.
  • the composrhon f ther comprises one or more agrochemscal iy active compounds .
  • a method ro so in another em odiment, a method ro so,.
  • th3 ⁇ 4 method comprises applying the bacteria! strain or composition to a seed, a plant or piaat. pari in s il
  • the composition further comprises om or more agrochcmic&ii active compounds.
  • ⁇ ⁇ ⁇ : ⁇ . ⁇ vO-ahrs drat modulate production of p m anv.
  • s are selected from tbc group consisting op Bottoms yioBqiq/Bck S2 ' Bacillus m 'hiiqulfac>i ⁇ BS205 Bacillus 3 A PA Bad/it,'.* ar: ; ykHiq fcsi ⁇ 'n LSSA L isA/fea nyk q Ja h? ABP2 8, dmbt- n;y >kqui ⁇ *ci s 22 FI . Batik w
  • baensrial strains t at contain the bdhA gene arc Bactiim
  • the bacterial strains thai contain the bdhA e e are selected isorn the group co s ti g of: Ba-kttxs *!?qkBqnifi?ckrii FA " ? BaclU mnyloi jtifbcem S2b84. BaciMi. ⁇ s nnioi!
  • lAA is a oarboxyhc aeld in hich die earhosyl p is attached through a methylene group to the C-3 position of an indole ring.
  • lAA is predominantly produced in cells oidhe ape>. (bod) and very yoang leaves of a plant. Plants can synthesize lAA fey several independent bmsynthetie adi a . Four of them star! from tryptophan, bra iacre is also a blosyntbetio pathway Independent of tryptophan. Plants msiniy produce IAA item tryptophan through indolc-3-pyryvlo acid,
  • I.AA hat many different effects, as all au ins do, such as indneing cell elongation and ee-i division with all sobsecpsefit efirnts tor plant gm th and development. On a larger stale, IAA servos ns .dgaabag moiecnle ncecssar)' ibr developmeni ot piant organs and coord!nahoo of growth.
  • the disclosure in ne embodnneot, relates m bacterial steams, compositions thereof, nd methods that produce IAA. in one embodiment., the disclosure relates io bacterial strains and compositions thereof that produce IAA vitro. In one embodiment, the disclosure relates io bacterial strains find compositions thereof that pr duc ⁇ i the resence of tryptophan.
  • IAA in plants is i creased by a percentage selected from e roup consisting ot from ;% to 3%, from As ⁇ .> 5 ' So, from.5% to 7%. front 73 ⁇ 4 t 9%, from 9% to I ] %, iron " : I ⁇ % to i ;3 ⁇ 4., front ] 3% to i SoA from i 5% to 17% s from 17% to 19% y from 10% to 7 3 ⁇ 4A and greater than 25% as compared fo an untreated cootrol.
  • the disclosure relates to a method o incre se pm ⁇ dtsction of plant auxins comprising applying at bast one bacterial stran that rod s IAA to a seed, a plant or plant pint.
  • method to increae production of plant auxin comprises applying a composition comprising at least one bacterial s ain that produces I AA to a seed, a plant, or plant past, in still another emb diment the method comprises applying the baetenal strain or composition to a seed, a plant or plant part in soil.
  • the composition further ' comprises one or more agroc emicahy active compound
  • the haetedal strains that produce IAA are selected from she group consisting of: !?om/0rs wyioiiQ ifhek s, .Aos-Vm oe/mo; Ae A, ⁇ d Bof: i?;s s bmits.
  • me bacterial strains that produce IAA are selected front the group c nsisting on AcmA:- ( >r: ⁇ Am -ma c ma--- 8A203 ⁇ 4A ty . ; ;-/o.,.
  • the bacterial strain that produces IA is BeeiH iichemfamk L2I.
  • the bacterial strain that produces !AA is Bacillus -ubdlis 3BPS. in one ⁇ - :id. ':.jir ! ⁇ - ⁇ «;. the diselesnre relates to bacterial strains, compositions thereot and rrrethods that contai a gene that encodes tryptophan synthase, ⁇ , which is the precursor for mdoic ⁇ 3 -acetic acid (lA A) production,
  • the disclosure in another embo iment the disclosure relates to hastens! strain;;:, compositions ere and methods that produce t y an synthase.
  • the bacterial strain?, compositions, and methods disclosed herein can increase expression of the ey. tP- gene in lants, h; one . 0%. hncnr expression or ' the ⁇ . ⁇ po e as plants is increased by a pereeninge selected from the group consisting of from i % to 3%, from 3% a. 5%. front 5% to ?%, from 7% 93 ⁇ 4, irons 9% to ! 1 %, born ⁇ % to ! 3H f firom 13% to 15%, from i 5% to i 7 , from ! 7% to 19%, Irons 19% to 25%, and greater than 25% as s-psivb to an untreated control,
  • the bacterial ste ns, compositions, and methods disclosed herein can increase the amount of tryptophan synthase in ptatts. fai one embodiment the amount of tryptophan synthase in plants is increased by a percentage selected fro i the group consisting of from 1% to 3%, bom 3% to 5%, ir m 5% so 7%, from 7% to 9%, from 9% to I i %, m i ! % to 3%, from i 3% to 15%, fro; a ; 5% to 17%, h'orn 17% to 19%, from 1 % to 23%, and greater than 25% as compared to an ent eated control
  • the isclosure relates to a method to increase production of plant assarts ii rtt riy rig apply ing at least one bacterial strain thai contains a gene that encodes tryptophan synthase to a seed, a pl nt, or plant part
  • a method to Increase production of plant auxins comprises applying a composition comprising at least one bacterial strain that contains a gene that encodes tryptophan synthase to a plant or plan? par .
  • the method comprises applying the bacterial strain or composition to a seed, a plant or plant part in soil, i ' o anoiber embodiment, the composition bather comprises one or tnore agroeherolcalSy active compounds- In another embodiment, the ores ansin s 1AA, in one embodiment the disclosure relates to a method to increase production of plant ansdns comprising applying at least one bacterial straio that oduces tryptophan synthase lo a seed, a plant or plant pan:, In another embodh-ncnp a method to increase r ducti n of plant auxins comprises applying a composition comprising at least one bacteria!
  • the method comprise applying she baetenai strain or composition to a seed, a plant or plant pari its sod. )n another embo ime t the composition bather comprises one or more sgroehemica!ly active compounds, in another embodiment the plant auxin is IA A .
  • the bacterial strains tha r contain a geae that encode nyptophan s n1.h3.se are Ba iii an;y/otiiiikfhciet?i.
  • the bacterial strauts ring contain a gene tha encode tryptophan s nthase are are selected from the group consist ins, of: Baciiim myioikp f kns ES%o Bacil!m Luuyioiiquifyckm 8S20S4, BacBins em?yk ⁇ i:qwfaek > t cot 7% Bact! m) vkg c ⁇ /u%#% teeife LSfbyob r3 ⁇ 43 ⁇ 4of/os
  • the root the organ of a plant typically lies belosv the surbboe of the soil
  • roots can also bo aerial or aerating (grovclng op abo e the ground or a- ;.v. ; dh a ov ⁇ , , ⁇ ' ⁇ . ⁇ ' ⁇ . ITaihermore, a stem normally occurring below ground Is not exceptional either (see rhissome). Therefore, as used herein, the mot is the won-ieal oon-nodes bearing arts of the plant s body.
  • the first rooi that comes from a a is called the radicle.
  • the four major funct ions of fo ts are: ⁇ ) absorption -n w.u., - and inorganic nutrients; (2) anchoring of the plant body to the ground and supporting k: ( a) storage of food and oao'i-o a> and ⁇ 4) vegeunave reproduction .
  • Roots often un l sen in storage of food and uaneato
  • the roots of most vasetdar plant species enter into symbi i i its cenain fungi to form mycorrhisas, and a large sng-,. of otber organisms Including bacteria also closely associate with roots,
  • the disclosure relates to bacterial sir3 ⁇ 4;ns. c mp siti ns thereof and methods that promote root ionnabon in plants
  • die disclosure relates to bacterid strains, eon-positions thereof, and aiethods that stimulate root format ion in pianos, hi one embodiment- the discl sure relates to bacterial st ains, c m sit o s thereof, and methods that Increase the strength or vigor of roots In plants.
  • the disclosure relates to bacterial strains, compositions thereof and methods that increase root mass in plants.
  • the disclosure relates to bacterial strains, compositions thereof and methods ih&r increase toot fonctioa in lan ?* including but not limned to: absorption of water nd inorganic nutrients, anchoring of the plant body to she ground, a d supporting it, stora e of food and nutrients,, and vegetative reproduction,
  • the bacteria! strains, compositions, and methods disclosed herein car Increase the amount of root formation in plants.
  • the amount of root formation in plants is Increased by a percentage selected b m t e group con istin of Irom 1 % to 3%, from % to ,53 ⁇ 4.
  • the bacterial strains, compositions, and methods disclosed herein can increase root mass in plants, bt one em od ment s root mass in lant is increased by a e centage selected tfom the gnaup consisting of from 1 % to 3%. bom 3% to 5%, f n-; y3 ⁇ 4 to ? ' %, from 1% to 9%. from 9% to 1 1 3 ⁇ 4, Sk>m I : % > ⁇ ⁇ I 3%, from 13% to ) 5% ; sVom ⁇ $% to 1 3%, h3 ⁇ 4m 173 ⁇ 4 to 19%, i: , ⁇ ⁇ 19% to 23%.
  • the strength of roots m plants is btereased by 3 ⁇ 4 percentage elected from - e group consisting of ( rn 1 % to 3%, fro 3% 3 ⁇ 4% ; fhs 533 ⁇ ⁇ 7%, from ' ?% to 3b% from 3 ⁇ 4 to I i %, ih>m ⁇ 1 % to ⁇ 3%, bom 1373 to 1 S%, horn j 5% to l 7%, from i 7% tc 193 ⁇ 4, jhsm i bps to 25%, and greater ihan 25% as compared to an nntreated control,
  • root function in plants is increased by a petcentage selected from the group consisting of from 1 % to 3%, from 3% to 33b, from 5% to 7%, ben e 733 to 9 , h a: 933 so 1 ! %., b m U % to 33%, from ] j% to i 5%, from i 3% to I ?%, thorn I %3 to i 3 ⁇ 4 t from 19% ro 25%, and greater than 25% as compared to an animated control.
  • the disclosure relates to methods to promote root formation in plants, to n embodiment, the method comprises applying one or more bacterial strains or eompesi ' tkms thereof to a seed, a plant or plant pad in n effective amouni to promote root formation.
  • the disclosure relates to methods ro stimulate root formation in plants, in one embodiment, the method comprises applying One or more bacterial strains or compositions thereof to a seed, a plant, or pbmi part in an effective amormt to stimoiaic root formation.
  • the disclosure relates to methods io increase root mass in plants, in one em odbrse n, the method com ses applying one or more acte ial strains or compositions thereof to a seed, a plant, or plant part in an effective amonm io increase root mass in plants.
  • the disclosure relates to methods to iresrease the strength or vigor of roots o plants.
  • the me d comprises applying one or more bacterial strains or compositions t ere to a seed, a plant, or plant, pari In an effective amonm to increase the strength or vigor of roots.
  • the disclosure relates to methods to increase root function in plants.
  • die method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or pi are part In an eiiesbve amount to increase root fancilon .
  • the seed, plant, or plant part described in Section C above Is a marigold
  • bacterial strains, siomposiiioos thereof, arid methods that promote root fbrraetiwt rose smnulation, erease the strength or vigor f the roots, or increase root function in a marigold are selected ova a the group consisting of: Bacillus io/iquifac ns, Ba illus lick nifor i-;, and
  • the bacterial strains, compositions thereof, and methods thai promote root formation, root stimulation., merease the strength or vigor of the roots, or increase root fun doo a marigold are selected from the group consisting ot: Bacillus m yh!iquifyo ⁇ 7233 ' i . Bacill s $ ⁇ ⁇ ⁇ ⁇ AH., toemhit m i ⁇ i tH si 3 A N, loeiftoi
  • composition comprising Baeiikis cmyloiiqiqf cie 22CP I atal I 5AP4, and methods thereof promote root fonmtt om root stimulation, increase the strength or vi o of the roots, or increase toot function in a marigold.
  • the seed, plant, or plant part descnbeej in Secti n € above is a tomato.
  • bacteria! strains, compositions t ere f, and meth ds that promote root fbraiadoro root stimulation, increase the strength or vigor of the roots, or Increase root function in tomato are selected from the group consistmg of; ciH and B dihi* ikhswfi>r i& t sad combinations thereof;
  • the bacterial strains, co sition thereof sod methods thai promote ; root formation, root stimulation. Increase the strength or vigor of the roots, or merease root function in a tomato are selected from the group consisting of. BocHhis v yioHiHii/acie 2014, actikxt
  • shoot refers to the serial portion of a plant Ineleomg the stem, ranc h;- ⁇ and leaves, The term “shoot” may also refer to now. Immature growth of a plant.
  • the shoot of a plant has many dmci ns including but not limited to photosynthesis, reproduction and dispersal, and food and water eondocbon.
  • the disclosure relates to bacterial strains, composhions thereof, and methods that promote shoot formation
  • the disclosure relates to bacterial strains, compositions j hereof, sad methods that Increase shoot height in plants.
  • the d!sclosstre relates to bacteria! strains, compositions thereof, and methods that increase shoot w dth m plants.
  • the disclosure relates to bseseria! strains, eompositiena thereof, and methods that stimulate shoot ftainstior? in plants
  • the disclosure relates to bacteria! strains, compositions thereof, raid methods thai increase the strength or vigor of shoots In plants.
  • the disclosure relates to bacterial strains, compositions thereof, and methods that increase shoot fenctiots In plants including but noi limited to; photosynthesis, reproduction and d!spersab and food and water conduction.
  • the bacteria! strains, compositions, and methods disclosed herein can increase shoot height ift plants, in otic eml?odfrneat, shoot heig In plants Is Increased by a. percentage selected front the group consisting fr m 1% to 3%, from 3% to 5%, front 5% to 23 ⁇ 4, from 7% to. n%, dons ; - ⁇ > > ⁇ > t ! 3 ⁇ 4 f m 1 17a to i 3%%Vosn 15%, from 15% to l 7%%Votn ⁇ to 191% from l %3 ⁇ 4 So 25* and greater than 25% as com are to untreated control.
  • the aeter stfgfes, eooipositlonsy and methods dis losed herein can increas shoot width Irs plants.
  • the baetesial strains. compositions, and methods disclosed herein can increase the ⁇ ⁇ . ⁇ of shoot formation in plants. Its one embodiment, the amount of shoot formation is plants is Increased by a emsiit ge selected foam the group consisting of b om 1 to 3%., from 3% to 5%, from %3 ⁇ 4 to 7% 5 from 7% to 9%, from 9% 3 ⁇ 4. ! I %, i ran ⁇ I % a 13%, from 1 3% to I 3%, from i % , to 1 7%, f rom 17% to 19%, mam 19% to 25%, arid greater than 25% as compared to an untreated control
  • the bacterial strains, compositions, and methods isclosed herein can ios re -a the sbength of shoots a -: plants.
  • Irs one embodbnem. th3 ⁇ 4 strength of shoots in plants is increased by a percentage selected irons the group consisting of from i % to 3%, from 3% to 5%, from 5% to 7%, iron? 7% to 9% from 9% to 1 1 %, from 1 1 io ⁇ .3%, from 13% to i 573. from 15% to 1 7% from I ?%. to i 9%, from 1 % to 25%, and greater han 25% as compared to an untreated control
  • the bacterial strai s,, compositions, and methods disclosed herein can increase shoot function n plants, io one embo ime t shoot bmetlon in plants is increased by a percentage selected from the group consisting of ' from 1 % to 3%, from 3% to 5%, h3 ⁇ 4m 533 to 770, from 7% to 9%, from 9% to 1 13b, from I % so 13%, from 1 % to 15%, f om ! 5% to- 1 733, from 1 7% to 1993, bom 1 % to 35%, and greater than 25% as eompsaed to an untreated control
  • the disclosure relates to methods to promote shoot ftmsatlon in plants.
  • th method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in art elec ive amount to promote shoot formation.
  • the disclosure in one embod iment relates to methods to increase shoot height in plants, fa one emb diment, the method comprises applying one or more bacterial sfnons or compositions themof to a seed, a plant, o plant part in an effective amount to inooaa.se shoot height in plants,
  • the disclosure relates to methods to Increase shoot width in plants.
  • the method comprises applying one or more bacterial strains or compositions, thereof to a seed, a lan or plant part in an ef ective amount to increase shoot width in plants,
  • the disclosure relates to methods to stimulate shoot formaiioii in plants, in one embodiment he method comprises applying one or more bacterial s rains or compositions thereof to a seed, a plant, or plant part in an cfleetivc amount to stimulate shoot formation.
  • the disclosu e relates to .oomod;. m ⁇ -; ⁇ ;,»? mo stioogi r vigor of s ts io plants.
  • ihc method comprises a pl ing ne or more bacterial strains or compositions thereof to s seed, a plant, or plant part in defective amonot to increase the strength or vigor of shoots.
  • the disclosure relates io meth ds to increase? shoot function in plants.
  • the method cmprise applying one or n, ⁇ ; ⁇ :. ⁇ baeten l strains or compositions thereof to a seed, a plant, or piasn port so an effective ism nni vo incs'ease shoot function,
  • Increase shoos height, increase shoos wi th, increase the strength or vigor otohe shoots, or increase shoo- function in marigold are selected mom the group consisting of: /be ; ⁇ ; ⁇ .
  • a composition comprising B c hts and iSAI promotes shoot formation, shoot stimulation, increase shoot height, increase shoot width, increase the :--oeroh. or vigor of the shoots, or increase shoot inaction in a marigold.
  • a composition eong:nbsmg .B «c//f3 ⁇ 4s amyia!!qtifhei ii * 22CPI and BSIS promotes shoot formation, shoo; stimulation, lnei3 ⁇ 4ase shoot height, increase shoo? width, increase the strength or vigor of the shoots, or increase shoot function in a marigold.
  • the seed, plant, or pkrss part esexbed to Section fJ above.
  • bacterial strains,, and compositions thereof that promote shoot formation, shoot stimulation, increase shoot height, increase shoot width, it5crease the ste th or vigor of the shoots, mcrevrce shoot fooeoon its a tomato are selected horn the; group consisting of: /fo. ; ⁇ 3 ⁇ 4. ⁇ ,
  • bacterial strains, and compositions thereof that prosrsote shoos formation, shoot stimulation, increase shoot height, increase shoos width, increase the strength or vigor of the shoots, or increase shoot function in tomato are selected from the groap consisting of: Bacillus
  • a leaf is Signed to cappme sunlight which the ant uses to nmk ⁇ food th wgb it process called photosyrnhesis. Most plants' food is made in t s ⁇ . . ⁇ l ave .
  • Leaf lengths, v widely from one plant species to the next. In addition, leaf lengths oat vary within a single plant.
  • the disclosure relates to bacterial strains, compositions thereof, and methods promote leaf formation in plants
  • the disclosure relates to bacterial strains, compositions thereof, and methods thai increase leaf length m plants.
  • the disclo ure relates to bacterial strains, composition? thereof, and methods that Increase leaf width in plants.
  • one embodiment ie disclosure relates to bacterial strains, compositions thereof,, and methods that stimulate leaf foruod ion in plants.
  • the disclosure relates to bacterial strains, compositions thereof, and methods that Increase the strength or vigor of leaves in plants.
  • the disclosure relates to bacterial strains, compositions thereof* and methods that increase leaf fanci ion in plants including bat not limited to pk>kawmhesis, reproduction and dispersal, and food and water connection.
  • the bacterial strains, compositions, and methods disclosed herein can increase leaf length in plants.
  • leaf width In plants is increased by percentage selected i on ! the rou consisting of from 1 to ' ⁇ %, from 3% to 5%, from 5% to 7%, io -n - ? % to 9%, from 953 to 1 1 3 ⁇ 4, from i I % to 13%, from 13% to 15%, from 15% io 173 ⁇ 4, from 173 ⁇ 4 to i 95% Irons 19% to 25%, and greater than 25% as compared to an nntreated control
  • the bacterial strains, compositions, and method disclosed herein can increase the amount of leaf formation in plants, in one embodiment, the amount of leaf formation in plants is increased by a eiWit ge selected from the group consisting of from 1% to 3% : , front 3% io 5 . from 5% na 7%, from 7% to 9%, from 9% to ⁇ 1 3, Irons I ! % to 13%, Pom 1.3% to 15%, from i 5% to !?%, from 17% to I ->%.. 19% to 25%, and greater than 2553 as compared to t untreated control.
  • a eiWit ge selected from the group consisting of from 1% to 3% : , front 3% io 5 . from 5% na 7%, from 7% to 9%, from 9% to ⁇ 1 3, Irons I ! % to 13%, Pom 1.3% to 15%, from i 5% to !?%, from 17% to I ->%..
  • the bacterial stratus, compositions, and methods disclosed herein can Increase the strength of leaves in plants, in one embodiment, the strength of leaves in plants is increased by a erce tage selected from the group consisting of from 1% to 3%-, u- -m >% to 5%, fro S% to 7%, % -m 7% to 9%, imn; 9% to i ] %, ⁇ : ⁇ . I ) % to 13%, test! I % to 1 S . bom 1 $% to 1 %% ftmo 1 %3 ⁇ 4 to 19%, boons 1 o% to 25%, a d greater than 25% as eompaosd to an untrea eontg:>f
  • the bacterial sprains, sompnsliions, and methods obselosed herein ear* increase leaf function in plants, in one emb di ent, leaf function in plants Is increased by a percentage selected frotn Pie group consisting of fPom 1 % to 3%, from 3% to 5%. from 5% to 7%, from " o.- to 9%, from 9% to ⁇ from I 1 % > ⁇ ⁇ 13%, from 13% to 1 %.. iron 15% io %%, from %% io 19%, from 19% to 25%, and greater than 25% as compared to an untreated control.
  • the disclosure relates to methods to promote leaf formation in plants, hi one embodiment, he method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant o plant par m an effective anton to promote leaf formation.
  • the disclosure relates to methods to increase leaf length in plants.
  • the method comprises applying ono or more bacterial strains or compositions tlmreof to a seed , a plant, or plant part in an effective amount to increase leaf length in plants.
  • the disclosure relates to methods to increase leaf width hi plants, in one emb di ent, the method comprises applying one or more bacteria! strains or compositions thereof to a seed, a plant, or plant part in an effective amount to increase leaf width in plants.
  • the diselosnre relates to methods to stimulate leal formation in plants, in one ennbodlrnem , the method eomprisms applying one or snore bacterial stra ins or compositions thereof m a seed, a plant, or plant part in an effective amount to stimulate leaf formation.
  • the disclosure relates io methods to Increase the - o enrol: or vigor of loaves in plants.
  • the method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in an effective amount to increase the strength or v igor of leaves.
  • the diselosnre relates to methods to increase leaf " function in plants.
  • the method comprises applying e or more bacterial strains or compositions thereof to a seed, a plant, or plant ri In an eiieetive am un to increase leaf function.
  • bacterial strains, and compositions thereof that promote leaf formation, leaf stimulation, increase leaf length, increase leaf width, increase the strength or vigor of die leaves, or increase leaf function in a marigold are selected from the group consisting of; Bacillus my!oiigitifacienx 22CP L « ⁇ ?/ ⁇ /,. ⁇ ; tti iquifa tefis ISAM, ftecithfS ⁇ httqtiijhce LS$ AOL
  • the -wasp oonsistmg ot i aswm i ⁇ yk--iiqv/ c '.
  • Backi &dwn /0mrfp f ba liiifs poiy yxa and combinations thereof.
  • bacterial strains, and composii n thereof thai promote leaf formation
  • leaf stimulation increase e f length, tnc-rease leaf width, increase the stren th or vigor of the leaves, or increase leaf I .
  • action in a tomato are selected rom the group consisting of; Bad/lus yloli /fh kfis BS27, Badik inyl ikikfa Boc/te awykkiq ifivke d b'sb Ikiciihis myh!iqikfac s LSSAOK Sooiiiv ⁇ !diquifack s ⁇ BP278, Bacikus amy!oHqniJ c BSI 8, Bac l in i iformk BAS 2, S iHi ikh ⁇ mfbrmk BL2 ⁇ ; i ⁇ mbacHius pofymyxa ABPI 66. and combinations thereof,
  • the disclosure relates to bacterial strains, compositions thereof, and methods that promote flower bod presence i plants, in one embodiment the disclosure relates to twererlal streams, cornposiUons -1 : ⁇ . ⁇ . moo and methods that promote bower bad tbrmaoon in pianrs.
  • the disclosure relates to bacterial straios, co positlorss thereof, and methods that increase the size" of flower hods In plants.
  • the disclosure relates to bacterial sbains, compositions thereof and methods that siintuhte flower bud growth In plants.
  • the disclosure relates to hacieriai strains, compositions thereof, and methods rhat increase the strength or vigor of slower bads i piarns.
  • the disclosure relates to bacterial strains, compositions thereof and methods that increase flower bud function in plants ncluding but not i united reproduction and dispersal.
  • the bacee ial strains, compositions, and methods disclosed herein can increase the number of flower bods in plants.
  • the number of flower bods in plants is increased by a percentage selected from the group consisting of from 1% to 3%, from 3% to 5%, ifom 5% to ?%. If om 73 ⁇ 4 to ⁇ 3 ⁇ 4, from 9% to 1 IH.from 11% to 13% ; Item 13% to 15%, from 15% to 17%, from 17% to 1 %, from 1 % s ⁇ 2s3 ⁇ 4,, and reat r than 25 as compared to an untreat d e mr !.
  • he hgeiebai sbtbns, eo oah ss., and methods rllse sed herein can increase the siee of flower bads in phmts. in one embcdirnent he slae of flower bods in plants is cmaaed by a pvu' ⁇ oa.;;;.:o selected from the g oup consisting of fr m ⁇ % to a% ; irons 3% to 535, from 5b!, to 7%., boa! 7% to 9%, from 933 to 1 ! 3 ⁇ 4. beta 1 ⁇ 3 ⁇ 4 to 1 3%. b oa . 1 3% so 1 5%, fro*-: 1595 to 1 73b if > ⁇ ; ⁇ 1 7% to 19%, boo; 1 3b to 25%. and greater than 25% as compared lo an untreated control.
  • the bacterial strains, coiy osibons, and methods d sclosed herein can inc ase the amou t of -flower bud formation in plants, in one embodiment, the amount of flower bud Isa-madon n plants is Increased by a percentage selected item the group• • •'m .mnp of from ) 3 ⁇ 4 to 3%, from 3%, to 5 , from 5% to ?% ? from ?% to 93b, from 9% te ⁇ i 3b b om I ! % to i 333, bom ⁇ 3% to 1 5* irons I 5% a ' l ?9b !rotn i ?% to 1933. front 1993 to 25% , and greater than 25% as compared to an entreated control
  • the bacterial strains, compositions, and met o s disclosed herein can increase the strength of bower buds in p nis- in one embodiment, f.h « strength of fese bads In plants is moi-eased by a percentage selected f om the group consisting of drotn 195 to 339, from 395 to 5%, from 5% to 7%, from 793 to ff % t ' roni 953 ⁇ 4 to 1 1 %, bom 1 ! 93 to 1393, from 1 393 to ! S95, from ) S% to ! ?3 ⁇ 4, bunt 1793 as 19%,, from 19% to 2593. and greater than 3593 as compared to an. untreated control.
  • flower bad function in plants is ntcreased by a percentage selected bom the group consisting of from 133 to 35b iron; 393 ⁇ 4 to 5%., from 5% ⁇ . ⁇ 7 , nvn: 7% to 995, bom 935 io 1 1 %, from i 155 to ⁇ 393 ⁇ 4, boas 33% to 1 5%, from i 55%o 1795, from ! 79 0 to 1955, from lab s to 2535, and greater rh a; 2595 as compared to ;m untreated comrof
  • the disclosure relates to methods to promote ilower bud formation.
  • the method comprises a lyin one or more bacterial strains or compositions thereof to a seed, a plant, or plant pari in an effective amount to ⁇ : ⁇ ⁇ Ilower bud fonyabion.
  • the disclosure relates to methods to Increase the nanaber of flower buds in plants, in one embodiment, the method comprises appl ing o e or more baei «ri»! strains or compositions thereof to a sees! a plant, or plant part in an effective amount to Increase the number of flower buds In pkmts,
  • tn o embodlnmat, dm disclosure relates to meth ds to increase the sme of flower buds In plants.
  • the method com rise applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in m effecti ve amount to increase the size of flower bads in plants.
  • the disclosure relates to methods to stimulate ilower bud formation its plants, in one embodiment, the one hod comprises applying one or more bacteria! strains or compositions thereof to a seed, I ban or plant part in an effeetsee amount to stnmdate f;ow3 ⁇ 4r byd formation. ' la one mttew& mt, the iml i esiaois to methods: to it m the slrsangtt ttt vigor of flower buds plants.
  • die disclosur relates to methods to increase flower bod Idacboo ⁇ plains, in one embodiment, the method comprises applying one or ntore b eteriai cede ⁇ or conutos ons thereof to a seed, a plant, or pfani part in m effective amount to Increase flower bud function,
  • the s e , piam or plant par described p3 ⁇ 4 Section F above is a uenseow.
  • n a marigold are Baci!i s my li uif ' cie .
  • flower bud stimulation increase in the number of ilowe-r ends, increase so the slae of flower bods, increase the srren3 ⁇ 4l:b or vigor of the flower buds, or increase blower bud -function ia a marigold are selected boo; the group consisting of: BaciHus imyh!kpiifi-iciem 22CPL B ciii amyhilqifcic ⁇ .Pwote i yiQtiqwfyckm BSiS, P nUnxiiius poiymyxa ⁇ 166, and c m inatio s thereof
  • compositions that promote slower bod forroadom flower bud stimulation, increase is the number of lower bads, i crease in the size of do buds, increase the strength or vigor of the flower i-o Is. or Increase flower bud function
  • a nmrigold comprise . ⁇ sc//Ao? oopa ' oOi ipoe'/eii.s 22CPI- and Bacill s iopyfedayipyfeePetv 1 SAP 4.
  • compositions that promote dower hud formation, flower bed stimulation, increase in the numb «r of flower bads, increase ia the size of flower buds, increase the strength or vigor of the flow3 ⁇ 4r buds, or increase flower bad function la a marigold comprise BcK-Him
  • compositions that promote flower bud formation, flower bud sihmbatbo, mersase in the neob3 ⁇ 4w of bower buds, increase ia the s?ae of flower bad-,, mcrease the strongth or vigor of the flower buds or inc ease flower bod function in a marigold comprise Bacillus ⁇ 5 AIM, and Ikteiti s a yioiiquifac BS 18. t * . O !m»nt frm ighd
  • he disclosure relates to bacteria; strains:, c m ositi ns thereon and mebasds thai increase total plant fresh weight.
  • the total plant ftss weight in plants is increased by a percentap selected moo the group aonsisiing of fmm 1% to %% from a% to 3%, from 5% t ?%, from 7% to 9%, from 9% to i 1%, bora j i % to i 3%, from 13% to i 5%, from 15% to ! 7%. than 1 %3 ⁇ 4 e> 1 from i 9% to 25%. arid greater than 25% s compared to an untreated control
  • the disclosure relates to eh ds ro increase total plant fresh weight
  • the method comprises ap l ing one or more bacterial strains or comp siUoB* thereof to a seed., a plant or lant part in an elRctive am unt to increase totai plans frmh wegh
  • baolenal aiming, and compositions t areoldhar Incrase total plant fresh wei ht are Ik lhiS kiitipiif ck S: Baa fs !khsiilfb is; and combinations thereof.
  • bacteria! strains, and compositions thereof thai increase total plant bosh weight in a marigold ate selected from the group ecawoainp of: Boaillxs u yloiiqnlfacie .?fr%. Bacillus a yioliq ifhc& v 22CFI %Pa;d «.v f yhliguifacie BS1 a. Ba H ⁇ amyie!iqui/ociem ABP27S, Bacillus oo%-: ; ba7oo BL2K sad combinations thereof.
  • the disclosure relates to bacterial strains, compositions thereof a ed'tods that increase total lant dry weight
  • the total plant dry weight in plants is Increased by a percentage selected from the :oeap eomistlnp of fern 1% to 3%, .from 3% to 53 ⁇ 4, from 5% ⁇ ;. ⁇ 7%, b n: 7% to 9%, from 9% to n% ; ifr3 ⁇ 4t 11% to 13%>, from 13% to 15%, from 15% to 17 ⁇ fr m 17% so 19%%k>m 19% to 25%, and greater than 25% as compared to an untreated eontrog
  • the disclosure relates to methods to increase total plant dry wei h, la one eafrodimeng the method e: ⁇ .. ⁇ . ⁇ : ns-cs applying one or more bacteria! strains or compositions thereof to a seed, a plant or plant part in an effective amount to increase totai plant dry eigh.
  • bacterial strains, arsd compositions thereof that increase total plant fesh weight in a marigold are selected from the group consisting of cslhis cirnylolU si ci-e - 22CPI .
  • Ba isl 15. ⁇ 4, Bacillus a ylviiaul c ii LSisAOL Bacillus c lcliqiyfi/iiens BS IS, and combinations thereof.
  • compositions thai increase total plant dry weight in a marigold com rse Ba tfhiS amykBkyBfycie 22CP1 end Badii rnyhiiquiyickiis BS ⁇ >k
  • Germination is the growth of an embryonic plant contained within a seed, ii results in the formation of the seedling, '
  • the seed of a vascular plant Is a small package produced in a rait or cone after the anion of male and female sex cells. All fully developed seeds contain an embryo and. in most plant specie some store of food reserves, wrapp in a seed COST, dome plants pfodaee a ing numbers oi seeds that lack eaibryos; these are called empty seeds d aever germinate.
  • Seed germination depends on both internal and externa ⁇ conditions. The most important eaternai factors kelude temperaure, water, oxygen and sometimes light or darkness. Various plants require different variables lor sncecssf l seed gertniaation. Often this depends on the individual seed variety and is closely linked to the ecological conditions of a la t's natural habitat,
  • the diselostae relates to bacterial strains, compositions thereof, and methods dial promote seed germination in on emb imen
  • the disciosme relates to bacteria! strains, compositions thereof and methods that increase the number of seeds that germinate.
  • the disclosure relates to bacteria! strains, compositions thereof, and methods that increase the number of viable seeds.

Abstract

The disclosure re la its to bacterial strains, compositions thereof and methods for reducing overall damage and losses in plant performance, health, vigor and yield. In one embodiment, the disclosure relates to bacterial strains, compositions thereof, and methods for inhibiting plant pathogens.

Description

This applicati n claims priority to and is a n n-provsioinal application of U S. Provisional atent appheation No.61 A 177725 filed December IP.2013, which is b wrp ed herein b reference in w, eoo.wg
FIELD
Bacterial strains, compositions, and method'; for redaciag overall damage a d l sses in plasu perionruusce, health, vigor and yield are disclosed. In one embodiment, bacterial sunlas, compositions, and ioe.hwl:-: for treating seeds, soil. plants, or pin: :· ao to reduce bsetcriaf i\mgai. and/or nematode damage are disclosed, in one embo iment, bacterial strains. corapositioas.; and meth ds lor treating seeds, soil plants, o plant parts to increase plant performance arc disclosed.
Plants are often the targets of attack from pathogens, sach as acteria, fang:, and nematodes. Slow, but steady, progress has beers made in the last few decades towards developing more efficient methods rtd compositions for controlling pathogen infestations in plants. Chemical pesticides have traditionally been nsed to control pathogen mfestadons. However, :·.·.·;··· are se eral disadvinaages to nsing chemical pestleid¾l agents, such as lack of seleebvity, persistence in the envnwraneni and toxicity.
Thus, there ice; been a long :eh need for environmentally friendly compositions and methods for controlling or eradicating yah- as n iaiestailon or? or in giants, re,, methods thai are selective, enviosaaentaliy Inert, neawpersisteuo and biodegradable, sac that fa well into pathogen n^an gsnvmt schemes,
Furthennorsy an increasing global population has placed nnprecedetsted demands on the agricultural industry. There is a huge need to increase pinna yield, vigor and productivity
In view of the foregoing, it would be desirable to provide bacteria t strains, compositions and methods that are useful against one or eve of harmful pathogens and can also im rove plant performance.
SUM A Y
in one embodiment, the dlsckwnre relates to « composition comprising one or more bacteria! strains selected from the group eopsisimg of B clHus mykiiquif cie . Baci!l iiehe forms, BaciUux suhkiis, and a nibaciil poiy iyxa.
In one embodiment, the disclosure relates to a composition eompnsmg one or snore bacterial strains selected ην··: the group consisting of tea new ?nyk}!i utf<*kii'Hs- §2'7 (NRRL 8-501 Sg Baciiius o y! l?gmf c fis BS2G84 (NRRL B-50013i, Beciihjs my iiq ifackns 15AP4 (ATCC PTA-6>07), f,a aw. : amy nqt fhckm 3AP4 (ATCC PTAfoSObg B isius mykBquifsckm 1 vs.yoi (NRRL - 50-104). Bacillus o ylol q tck ΛΒΡ278 (NRRL B-50034), Bacillus amyBBlquBack 1 13 (NRRL B-S0500). Bw iaam iiquij chw 9lZ{MKkL B-50508), Bwiiim am iiqiffacfem 22CPI fATCC PTA-650P), Bacillus (im Uquifadem BS!S (NRRJ BA'.-OgWt Bacillus lie en irnti* BA842 (NRRL B- 50310), Bacillus lichenif mii BL2I (MRRL B-50134), Bacillus s bttlts J ¥$ (NRRL B-50SI g and As^A^ R/sY-/-fg ;go o ABI-goo ONRRR Β-502Π g
In yet another embodiment, die islcosrue relates to a conge tio , comprising at ast two bacterial strains selected Rom the gtotsp consisting <>ΐ Bacillus amyloBqkfhcle . Bacillm Bcheaiiarr , Badlh^ s bBiB, and Pacalbiicillm pafy yxa in an effects v« amount io achieve an effect selected from the group coBSisting of: inhibit growth of a plant pathogen, improve plant performance, improve plant yield, improve plant vi or, increase ph sphate svidia ljUy, increase production of a plant hormone, increase sbrnd count, increase root formation,, increase shoot height in a plant., increase leaf kngdi of a plant, increase flower bud formation of a plane in rease t al plant fresh weight. Increase total plant dry weight, and increase seed germination. The strains can of the same species or different species.
In aaoiher eiwbr-dimotn, the composition further comprises a hioooniroi agent selected from l « group · οο-ο·. ong of bacteria, Rmgi, yeast proRrzoas, viruses, eotomopsihogefdc n-ooao-Oi- botanical extracts, proteins, secondary metabolites, and mrtocitlants.
In yet another embodiment die oomposinon comprises at least two strains selected 0 an the group consisting of: Bacillus amykaiqui/ackas BS2"l (NRRL B-S015), Be, .7.0.0 amyi /igui fastens BS20S4 (NRRL BA00I3) Bacillus myloligw/hc ss 15AP4 (ATCC PTA-OaO/f. Baalim
amylaliquifiadens 3AP4 (AfCC FTA-0505), Bac-ca.- a y lfaek Id o.vd ( RRL B-50504), .Bacillus a y llqidfaaem ABP278 (MRRL B-gSOgBaf Bacillm amykBlq tfhc m 1013 (NRRL B- 50509), Baviiius
Figure imgf000003_0001
918 (NRRL B-50508), Bticiliw amy Bqdfackas 22CP1 (ATCC PI A-650S), Bacillus amykBqiqfac is BSI8 (NRRI B- 50033), Bacillus iichenlfartvis BA842 O R L B- 50516), Bacillus h eniforntis BL21 (NRRL Β-5 Π4), Bacilli sabBBs 3 P5 (NRRL B--50R10), and B;.oo54;,.-- w: p.gson Λ.ΒΡΙ66 (NRRL B-50 JI) and con-^matioss thereof
in another cndx iimeoi, the composition c m rises Bacillm .aacB- ga yd-. · ·.···· 22CP1 and Bacillus amylaik i/ac x I 5AP4. !rt still another e b druent the composition comprises Bacillm ainykliquifitckas 22CF i rid i -/do amyloHguBackas BS ! 8, in yet arsother enRsa-drmeot, the composites* comprises Bacillm my tq&ifyck-m BSI8 and B ctHus amylollqiBfack 15AP4.
In another embodiment,, the composition ioono: comprises one or more agroehenneai!y a t ve compounds selected IVom die group consisting of: an insecOcide, a fungicide, a bactericide, and a nemaiicide. In still anoterft embodiment, its? composition further comprises a compound selected from the group consisting of a saRmer, a Ηρο-ehltooiigosaecharide, an Isofiavone, and a ryanodine receptor modulator. lo we embodiment, the composts n comprises one or more agfcshem ically active eornpoands, wherein one compound Is ryoaxypyr. In another embodiment the conges ion comprises one or more agroohemicaliy active compounds, wherein one c- ope and is cya wpyr, |.n another embodiment, the composition -. ·νορ;νν> both rynaaypyr arid eyazypyr,
In another em odimen , the composition further comprises at least one seed, plant, or plant part, in one embodiment, the seed, plant or plant part is genetically modified .
in one emb diment the compostion inhibits the crowd'! of one or m e plant athogens including bm n t limited to & bacteria, a fungus, a - irus:. a protoaea, or a nematode.
In another embodiment, the eornposbon is in an effective amount to improve plant performance including but ftot hmi ed to mcreased root, formation, increased root mass, increased root function, increased shoot height, increased shoot function, increased flower bud presence, increased flower bod formation, increased seed germination, increased total plant wet weight, and increased total plant dr.. w eight.
In nod'O embodiment, the disclosure relates to a med'sod comprising spplymg a composition comprising one or more bacterial strains selected from the group consisting ϊ ΒααιΊαχ
Figure imgf000004_0001
.ooet/te liche fbrnii, B dl s ώΐίίία, and Pae ibaclli poh yx
In another embodiment, the disclosure relates to a method comprising applying a composition comprising one or more bacterial strains seieeled from the group consisting oi .0. ;. who iyioikpjifiH.' 'i- . .f a o7w chemfbrf : .Pa .ffw . -J .O/W -.no/ 'v.vvwa /a,. -: ;·.,.>.· >. >.g-. ·;γ.· to a seed, a plant, plant pari or sod Irs an effective amount to achieve ar? erlbct selected iforn the goaup consisting of inhibit a plant pathogen, improve plant performance, improve plant yield, improve plant vigor, increase phosphate avaliabiiity, increase production of a plant hormone, uictease root fo mtknv increase shoot heigh- in a piano increase leaf length of a plant, increase flower bud formation of a plant, increase total plant fresh weight, increase total plant dry weight, and increase seed ermi tion.
In yet n ther embodiment, the disclosure relates to a method comprising applying a composition contprisiog at least two bacterial mams selected from the group consisting of Saei!iiiH
Figure imgf000004_0002
m ;,.·;.· 0.0 i heni hnm. Backlm subiilk, wag P enib ciiim pefywym to a seed, a plant, plant pari or soil in an effective amount to achieve an effect selected orn the group consisting of inhibit a plant pathogen, improve plant performance, improve plant yield, improve plant vigor, increase phosphate availability, crease production of a plant hormone, inewase root formation, -ncrease shoot height in a piano increase leaf length of a plant. Increase llower bwd formation of a plan!, increase total plant fresh weight, increase total plant dry weight, and increase seed genninailon. The strains can be of the same species os< different species- In another embodmen, the method com rse applying a composition further comprBmg a biocontrof agent, wherein the bioeoniroi agent selected f : a de g oup c nsistin of bacteria, hmgi yeast, protoi-oas, viruses, srmsmopathogestic nematodes, botanical extracts, proteins, secondary metabolites, and inaoculents.
In yet another embodiment, the method < · >:Τ:ροο applying a com osiio bather comprising at least ns-o strains are selected irmn the rou consistin of: Baillus ^loU talacie BS2? (NRRL ~ 50 5). Bacillus amyl uifackns BS2084 (NRRL B-5QGD), Bacillus myloliqtiifac m 15AP4 ATCC PTA-650?}, &adh<s a l liqulfaciem 3APd (ATCC PTA-6 6), Bacillus ur-rylonq if cie LSSAOI (NiiRL b b 101;. gacilim myloh'quia iens ASP278 (NRRL B- 50b34\ Bacith amyteliq iftteterts 1013 (NRRL B-50509), Bacillus a yhliqukaaens 91$ fN'RRL B-5G508), Bacill amyloH.]uif ciew 22CP1 (ATCC PTA-65G8), Bacitl iwiollquifyck BSI8 (NR 3 B-50633)} Bacillus iicheniformis BAS 2 (NRPi. C-Ac3dny R.-t ;HU .·. ,oA-.ao Hi .:{ (HRF) P--b>R4> R.-: BR> .mebo -BPc <NRFl B- bRiO), and Paenwa ill potywyxa ABP1 6 (NRRL Β-502Π) and combinmions thereof.
In soli another ernbodunem, the method comprises applying a composition eomprsmg .BoRRs cmn'hHquiJa ris 22CP! and Bacillus ^ldl uifyciens 15AP4,
In still another eodsodnriem, the method comprises applying a composition comprising Bacillus a^loiiqmfadc 22CP!a;¾! ¾ea%s a/ttyloiiqtttfiKtem BS1S.
In sul! a ther embodiment, the method comprises applying a composition comprising ¾ici¾«; my liqxifixc& BShS and B l'hs amy llquifhc m ISA ..
In yet another embodiment, the method comprises applying a composition: comprising one or more agr chemicsUy active compounds selected Bom the group consisting of: an insecticide, a fungicide, a bactericide, a d a nematieide,
fe still another embodiment, the method comprises applying a composition Birdie r comprising a compound selected R m she group consisting of a saiener, a !ipo-ohitoollgosaccharlde, isoflavone, and a ryanodine receptor modulator.
in one embodiment, the method comprises applying corn-position comprising one or more agKfcbemical!y active ciits otwsds, wherein one compound is synaxypyr, in another embodiment, the method comprises applying a composition comprising one or more agrochemRaSly active compoun s, wherein one compound s cya¾:ypyr, In another emb diment, the method comprises applying a composition com risin rynaxypyr and eyaxypyr.
In another embodiment the method comprises applying the composition in an effective amount R; inhibit growth of a plant pathogen, including but not limited to bacteria, a fungus, a nematode, a virus, a protozoa. In another embodimeng ihe method c mprises apple ing e composition in an effocii ve amoom m bo ove plam psi Bce n hsd ^ bar not Imihe-d to increased root formation, increased root mass, increased root bincbon, increased shoot height, increased seed germination, increased shoot batchon. ifH-mased Bower bud presence, increased flower bnd formation, incnaased total plant wet weight, and soereased total pimt dry weight.
In one embodiment, t e <h:- k-.nr rel tes to an isolated Aoe/bow shram for improv ing plant performance, plant vigor, or plant yield, wherein the Bectihit stain comprises the BdhA en*, and produces :od- AA> accde , ·ο
Irs one embodiment, ;ίκ disclosure relates to an isolated Bacillus strain for improving plant performance, plant vigor, or plant yield, wherein th« Bacittm strain comprises de trp B gene, md produces lndole-3-aeetle acid.
In one smbcxfees&t the disclosure relates to an isolated Jfc_%¾* strain lor improving plant performance, lant vigor, or lant yse!d wherein the Sa iH strain comprises the phoP gene, and has the ability m solnhiliA? phosphate.
In one embodime t the disclosure relates to an isolated .bbmb/m strain for improving plant performance, plant vigor, or plant yield wherein the JntcdAct stride comprises the phoio BdhA, ¾nd t pAB genes.
la one vmi.ve mem. the disclosure relates to an isolated Sue?! fas sirarn for improving, plant performance, plant vigor, or plant yield wherein the Ecsciikfs strain produces indoiew -acetic acid and has the ability to sobrblii e phosphate.
Exemplary embodiments are illustrated in the cc m nying drawings,
FiCA 1 is a photograph of ins pmoP PGR product (134 base pairs (bp)) on a 1% agarose gel Samples were loaded in t e following order f om kit to right: 100 bp nolec &r rnler, bAebb.v
a yt .n maciii FZB42 {positive control), £ colt 0157 (negative control), LSSAOb 22CP1, 1SA-F4, 3A.-P4. BS27; BSIS, BS2084, ABP278, BA842, BL2L ASP166, and 100 bp molecula ruler.
FIG, 2 is a photograph ofthe AbA PAR prodtmt (336 bp) on a ]% agarose gel, Samples were loaded In the foilowmg onier from left to right: 1 0 bp molemnar ruler, I A2B42 (positi e toniroil A 0157 (negative consol), LSSA01, 220PP i 5A-P4, 3A P , BS2 v BS; A BtAOIPb ABP27A BAB42. BL2?S ABP!dd, and 100 bp molecular rtder.
FIG, 2 is a graph depicting the standard curve and trend line ofbAA standard dilutions: 0, 74*124, 15.625, 31,25, 62,5, 125, 250, and 500 opnr FIG. 4 s a hotograph of th tigoAB PGR product (2,069 bp) or* a ! % agarose gel . Sampl s were loaded in the followin order from left to right; i 00 bp molecular ruler, F2B42 (positive control), E. coli O! S? (ne ative comroi), LSSA0 R 22CPL 15A-P4, 3Λ-Ρ4, B¾27, Bb | 8? SS20S4, ABF27S, ΒΑΪ42, BL R AB 16G nd 100 bp molecular ruler,
FIG. SAG is a i t re of the right bad flower stage.
FIG 5A-2 is a psoow of the ' cracked" bod flower sta s,
FIG - '■·.. -.·· is a picture of the "ρο !" of color flower stage.
FIG. SA-4 rs a icture of the folded petals flower stage.
FIG. SA G is a picture of the open flower stage,
FIG. 58 s a picture indicating whore the (lower uds were removed at the natural indentation of bod formation (biacT arrow) tor reproductive dry weigh; measurements.
FIG. 6 G a line graph depicting da. average percent genninarioa of corn seed grows; is; the presence of iioorf jov treatment or control.
FIG, 7 is a bar graph depicting the average seed hag dry weight of com seed grown in the presence of B cHi treatiuent or control.
Before explaining embodiments of the invention in detail, t s to be under tood that the bacteria; strains, compositions nd methods are not limited in their application to the detahs of construction and the arrangement of the components set forth in the following description or iihwtrated in the drawings and Figures. The bact ial strains, compositions and methods are capable of other embodiments Of being ps acrieed or carried out in w¾Hoos ways. Also, it is to be understood that rise phraseology and terminology employed herein Is for the purpose of description and should not be regarded as limiting.
Definitions
Unless defined otherwise, ail technical and scientific terms used herein base the same meaning as commonly understood by one of ordinar skill in the an to which this disclosure belongs. Singleton, or id. , DICTIONARY OF MICROBiOLOGY AND MOLECULAR BIOLOGY. 20 ED... John Wiley and S ns, New York ( 1994 c and Hale & Marhano T'H'f: HARPER COLLINS DlCTIOelARY OF BIOLOGY, Har er Peren ial, NY ( 1 ¾1 ) provid one of skill with a general dictionary of many of the terms sed in this disclosure.
This disclosure is not limited by the exemplary methods and materials disclosed herein, and any methods and materials similar or equivalent to those described herein ear; he used in the practice or testing of embodiments of this disclosure. Numeric ranges are inclusive of the onmbers defining the range. T e headings ro ided herein are sot nnbtatsons of the various aspects or enfaorl hsients of his discl sure, which can be had by reference to the speoliksuion as a v-d - sv
Other definit ons of terms may appear throughout the specification. It Is TO be iuidersiood that this disclosure s not limited to particular embodiments described, and as such, additional embodiments may, of course, vary, it is also to b understood dial the terminology used herein is bar she purpose of doses bres! particular
Figure imgf000008_0001
only, and is no; mtendod a - be limiting, since scope of she d isclosure will be limited only by the appended claims ami equivalents thereof
The ru rses'lcal ranges in this disclosure are approximate, aod thus, may include values outside of dse rasge e:nles.3 otherwise usdtceled Numerical ranges include ail values from and Including the lower and the upper values, in increments of one unit, rovided that there is a separation of at bast two units between any Sower valu and any higher value. As an e ample, if a compositional physical or other pswpcrry, such as, for evampie, nubecsdar weight, tesnpefaSrue, ore, . Is Irons 100 to 1 ,000, is is rmended that all Irsdw ideal va lues, such as 100, i O i , l Od wto. , sad sub ranges, such as 100 to 144, 155 to I 70, b o to 200. a. a . are expressly enumerated. For ranges c tain n values which are loss than one or containing fractional numbers greater O ca; one (e.g., 1 . 1 , 1.5, o;. '·, one unit is considered to be 0.0001 , 0.00 L 0.01 or 0, 1 , as appropriate. For r nges containing .Tads digit num ers less than ion to. gy ! to 5), one unit is typically considered io be 0. ! . These are ou!y examples of what is specific-ally intended, arid ail possible combinations of numerical values between the lowest value and the highest value enumerated, are to be considered to fee expressly stated m this disclosure. Numerical ranges ar c prov ided within this disclosure lor, among other things, relati ve amounts of comp nents m a mixture, and various teo peratnre and other parameter ranges recited hi the methods.
As used herein, Asd red ulster" refers to the action of introducing the imin and or composition to a n environme d in need of pathogen inhibition or improved plant performance.
As used herein, the ierm "agrochemicaiiy active compound" refers So any subsn-mee tsOat is r m¾. be customarily used tor treating plants including but not leaned fungicides, bactericides,, mseef aides, acarseides, uernaiieldes, inol iosekades, safeners, plant growth regulators and plant nutrients as wel l as tnlceoorgaeisin ,
As used herein, a ccsrnpositlon may be a liquid, a heterogeneous mixture, a hosn geneous mixture, a powder, a solution, a d!sperskav lyophilieed, freeee-ds ed, or any combination thereof.
As used herein, :Affc>.a ive amount" refers to & c-uantity of bacterial strain or cosnposltion sufficient to inhibit rowt of a pathogenic mk; m r n i¾s or to impede the rate of growth. In another embodiment, the term ' effective &mo«»r" refers to a quantity of bacterial strain or eomposltlon sufficient to improve plant perfommnoe. It Is understood thai an effective a?¾o«nt of bacterial strain or composition may not reduce the numbers of pests/paihogeus, but is effective in decreasb)g damage- to plants as a result of a pesi/pathogen. Accordingly, the efficacy of s treatment can bo determ ined via any direct or indirect sndpoims. For example, a pesheidaby effective amount may redsoe isafhogen damage to s eds, roots, shoots, or foliage of lan ; that are reated compared to those that are w e u -
As used herein, %inproviis or iiKressing p ' mi perforomnee" rotors fo improving r increasing the yield of the plant r irapmving the vigor of the plant or improving bods the yield and ie vigor of the plant.
As sed herein, " improving or increasing the yield'' of a plans reiatss to an increase in the viekl of a product oi' the plant by a measurable amount over the yield of the same product of the plant prodaced under the same conditions, but "w i hout the application of the sobject bacterial strain, composition or method. la one embodiment the yield is increased by at least from about 0,5% to about 1 %. In auodier ernbodarisnr the yield is increased by at leas from about 1 % to aisoai 2%. In another ;.o. n P ew-:;;, t o yield is increased by at beast if - en about 2% to about 4% . in one e m hod i rn era , the yield is increased by at least about from 4¾ t ahead 5%. I anot e embod iment > % yield is increased by at least about from 5% to about 10%, In another emb diment the yield is increased from about 1 0% to about 20% or greater than 20%.
Yield san bo expressed in terms of an amoam by weight or volume of a product of rhe plant on some basis, 'the oasis can be es ps eased in terms of t ime, grosvbsg area, weight of plant-:; produced, amount of a raw ma terial used, or the like.
As used heeeiio the phrase " Improving or increasing the vigor" of a plant: relates to an increase or a pprovement or the vigor rating, or Use stand fthe number of piano:; ow unh of area), or do; piani height, or the plant canopy, or the visual appearance (such as grows; leaf color}, or the- root raon or root function, or root formation, or root mass; oi' emergence, or prole content, or i creased ttllstrng, or bigger leaf blade, or longer leaf length, or wider leaf, or increased flower bud presence or formation, or less dead basa l leaves, or stronger hhers, or less ferrihzer needed, or iess seeds needed, o~ more productive til lers, ss ear lier dowering, or early grain maturity, or less plans verse ( lodging), or irrovawed shoot growrh, c-r increaoed shoot hssght or increased shoot llmetiom or earlier germination, or Increased seeel germ ination, or increased total plant wet weight, or increased total plant dry weight, or dec eased use of fWlll isaiOO or decreased nse of additives tor growth, or decreased use of conventional pesticides for pes? cooiroj or aw. c ombination of these laetors, or any other advantages familiar to a person · kid ,o in the art by a measurable or noticeable am unt over the same factor of the plant posdaeed under the same conditions, but without the application of the subject bacteria! scrams, compositions or methods.
As used herein, the term "inhibit"1 refers to destroy, prevent, control, decrease, slow or othemioe Interfere with the growth or s rvi al of a pathogen when compared to the growth or surviv al of the pathogen in an untreated control. In one embodiment, to "inhibit" la io desrroy, prevent, control. decrease, sl or otherwise interfere with ihe growth r1 survi al of s a hogen by at least about 3% st km. abo t 100% ; or any value In between for example at least about 10%, 15%, 20%, 25%, 30%, 55%, 40%, 45%, 5 , 55%, 6 55, 6S%, 70%. 755% 80%, #5%, <%% 05%, «9 , or 10055 when compared m the growth or survival of the pathogen in an animated control. The amount of inhibition can be me sured as described h ein or by other methods knows? in the art.
In another aspect, to "Inhibit" is to destroy, psev-vm. corneal, decrease, slow or otherwise interfer wdh the growth or sun - wd of a pathogen by at least abooi Moid or more, for example, about 1 -5-fold to shout ! 00~fold, or any vahne in et een for example by at least about 2.0, 2.5, 1.0, 3.5, 4,0, 4 · 5 ,0, 5.5, 6.0. 6.5, 7.0, 7% 8.0, 15, 9.0, 9.5, ϊ θ, O. 20, 25, 30. 35, 40, 45, . 55, 60, 65. 70, 7$, ¾% eS, 90; 95-fo!d when compared % the growth or survival of the pathogen in an mdreated control
a one embcxll ent. Inhibition of die gro wth of a pathogen occurs smmediatejy, In another aspect inhibition of the growth of a path gen occurs one minute after, 30 minutes after, 45 minmes after, one h- r a ter, earn hours after, tons hoars after, sis hoars af!er, w ew hoar;? after, eigh een hour* after, or one day or more after a bacterial strain or composition d s losed herein s applied k- a plmi material.
In one aspect, inhibition of the grow th of a pathogen lasts lor ο*· provides protection for greater thtm one or more days, two or more days, three or more days, four or mo e days, five or more days, one week., two weeks, three weeks, one month or snore after a bacterial strain or composition disclosed herein is applied to the subjee> m terial. In aaoiher embodiment, inhibition of the growth of a pathogen lasts from W K- to severs days, dose sewers to ) 4 days, frorn 14 to 21 days, or from 1 to 30 days or more, in another embodiment, inhibition of pathogen growth lasts until a plant material is consumed or discarded.
As used herein, the term "liquid" means a substance In the fluid state of matter having no fmed shape but a fixed volume. Liquids may be liquid at room temperature and pressors.
As e$ed e e n,, the term "paihogen" Includes bet is not I med to pathogeme fungi, bacteria, and nematodes, as well as arthropod pests such as insects, arachnids, ehilopods, and dipiopods. The
"pathogen*' may be present at an point in the lifetime of a product for example, originating from one or more of the ollo i g: the environment from which the product was obtained and/or dw microbiological medio of the product in its raw or unprocessed state (e.g. native to the product) and/or any handling and 'or processing steps m; - s the Ki ecto'eness.aneffoeiw eness of packaging andon- storage conditions of the product.
As used herein, the phrase "pathogenic ntlcroorgtunsm" refers to a 'microorganism that is capable of causing disease in human, an animal or a piarrt. The "'pathogenic microorganism'' * may be present at any point in the lifetime of a product, for example,, originating from one or more of the following: the eovh omnent f o??:) which the product was obtained and/or dee microbiological quality of the prodecs m its >:·-:·-.- or nnproeessed stats (e.g. na ive to the product) ->nro-r any handling aad/or process n stops and/or the e!!ee i eness/ i iibct v a sa of packaging and o? storage eondhions of t e product.
As w,ed herein, the seam "plant" refers u- ail glacis sad plsm po ulatio s, s ch as dessrabie and undesirable wild plants, oakivars and plant a ieties (whether nor proteciable by plant varlegv or l in breeder's riaius). Cu tlvars and plant varie ies can be piants obtained by conventional propagation and breeding oterhods that can be assisted or sopplemeoied by one or more biotechnologieal methods such as by use of doable baploids, protoplast fusion, random ami directed o-miageoesis. molecular or genetic markers or by bioeoglsneer g and genetic engineering methods.
As useo herein, the term "plant parts" refers to ah above ground and betosv ground parrs and organs or plants sacs as shoot, leaf, blossom and «x;g whereby o example le ves, needles, stems, branches. btossw'ns, banting bodies, irons and seed as wed as rooty aider:- coons and ddaomes are nschsded. Crops and vegetative and generativ e propagating material, for example endings, eotms, rhie'omes, ushers, runners and seeds also belong to plant parts.
As used herein, the terra "plant heakh" is to be nadcrstood to enoie ¾ condition of the plant and/or hs products which is deterrnmed by several Indicators alone or in combination wit each other snch as yield (e.g. increased biomass ami/or increased content of valuable ingredients), plant vigor (e. . disproved plant growth and/or greener leaves ("greening effect"}), qualify (e.y. improved content or composition of certain Ingredients) and tolerance to able-tic and/or biotic stress. The above identihed indicators for the health condition of a lant may be interdepeoden! or may result from ea h other.
In one as ect the term "'prev ntin " refers to the pathogen contamination of a product to which the strain or composition is applied and has an evtended shab b y- and/or increased time frame before a specified amoum of eootaimnant is present. In one embodiment, slaiil- li!e and/or time frame Is extended mai o< n¾creased when eoatpared to a control product that does not have a strain or a composition applied.
For 0K.ample.. when the eomandnant Is a pathogenic microorganism {e.g, a pathogen bacterium) the "specified amount of contaminant'' may be the level at which a product is deemed not to be sale for use by. for evarnpie. the FDA . in sotoe Instances, depending on the pathogenic microorganism, the specified amount of contaminant may he aero. This may be the case whet; the pathwgenic ndamwrganisro is listeria spp. for example, in other instances, the specified amount of contaminant may be less than about iOO CFL' g o mi or less than about 10 OA * g or mh sach as when the pathogenic bacteria is e,gi f A ii spp.
As used hereby the tears "reducing" in relatio to pathogen contaminant means that die level of pathogen growth and/or speed at which a product spoils ts reduced when compared to a control product to which no strain or composition has been applied. in one aspect., the terms Avxluoe" and 'Ye ocmg" may be used Interchangeably w ith the terms "inhibit" and "inhibiting.'' As used hereby a
Figure imgf000012_0001
.has as least S0% identity' of genetic sequences with e disekcsed strains using random amplified polymorphic DHA polyi«erss« chain reacti n (RAFD--PC ) analysis. The degree of identity of genetic sequences can vary, lit s e embodiments, she variant has at feast sy¾y <>{>%, 5%, 96%, 97%, " - or 99% identity of genetic sequences with the disclosed strains using RAPD- PGR analysis. RAPD analysis can be performed nsang Ready-to-Go' '" RAPO Analysis Beads
(AToets am iosciences, Sweden), o cb are desipted as preornlxed, pre-drspensed reactions for per form bg RAPD analysis.
As used herein, the teroi "synergistic effect" is understood to refer to that defined by application of the Tammes oosahod, ·: I -ονοηοο M, L.y Asohobs, a graphic representation f synergism in pesticides". Nether!. I. Plant Pathol. 70. 1964). 'The term Ayoergstse effect" is also understood to reter in particular to that defined by Colby's formula (Colby, S, R., "CAlculatlng synergistic and agtagonisiic responses of herbicide combinations". W eds, I S. pp. 20-22, ! 96
As nsed herein, the term ^viable" re As-, to a microbial eett or spore that is metaboboslly active or able to diff rentiate. Thus, spores are boable" whet) they are dormant and capable of germinating.
The disclosure relates to bacterial strains, compositions and methods of using the strains. In one embodiment, the disclosure relates to isolated bacteria! strains, compositions and methods to inhibit plant pathogens. Including but not limited to bacteria, fungi and nematodes. In another embodiment, the disclosure relates to bacterial strains, eornposbona and methods for improving, plant peA cons mo - In sub another enirx-dnnemy the disclosure relates to bacterial sRains, com osi i ns arid methods lor impreeeing plant vigor. In yet another mbodiment, the disclosure relates Pa bacRrial strains, compositions and methods for Improving plant yield.
In still another embodiment, the disclosure relates to bacterial strains, compositions and methods tor lobhoim: plant pathogens, na amather embodiment, the disclosure relates, to baciersaf strains, compositions and rnethods for inhibiting plant pathogenrc bacteria- in yet cm tiier e bo iment, the disclosure relates to bacterial strains, com ositions, and methods tor inhibiting plant pathogenic fungi. In another embodiment, the disclosure relates to bacterial simitis, compositions, and methods For inhibiting nsma tsdes.
I, o bCytNSt
The disclosure relates to bacterial strains, compositions and methods of using the strains, in one embodimeno the disclo ure relates to a composition comprising at least one bacterial strain disclosed herein. In another emixeiiment, one or more bacterial strains disclosed herein inhibit plant pathogens, including but not limited to bacteria, i'ungi and nematodes. In amaher embodiment, one or snore bacterial strains disclosed herds improve plant performance. In sell another embodiment, am or more bac-enai strains disclosed hereio improve plant vigor. In vol another embodiment, oas or snore acterial strains disclosed herein improve p!sui yield.
In one embodiment, the bacterial strain' s} are selected from the group consisting of: Bacillus !ti !enqulBick , Bitc ks s dBix, :■■.;< .· ¾·· ikden or k, u*td "ami bad!i pofywyxa, and
& o oi biostions the roof
io another embodiment the bacterial strain(s) are Baeill imtyBBHnBfacm selected tY m ibe group c nsisting of; oa'i is at BiqvBBcie BS27: BadBus a yloiiqtqhckm BS20¾4, Bacillus ryk&quifii kM Ϊ5ΑΒ4, Bacillus yBBiquBhciem AIM. BaciBm ei ykBq Bhde LSSAOI, B di fs nmyloiiquif d^ns A P27& IkaB!lus mnykBq ilade 1013, B U a yloiiq mck 91$, BacBBis mf!ylo qdfadefi esCPg and a illi amyloliq lfac m BSI8, and combinations here f.
In another embodiment, the bacterial stfa»s(s) Ba Hw Bchedfarmis selected from the group eonshahsg oft Bacillus Bchmi/or k BA842: and Bacillus ikkcni/bcmk BL21, and combinations thereof
In a other embodiment, the bacterial strain Is Sacittm subtilte 3BB5.
in yes another embodiment the bacterial strain
Figure imgf000013_0001
yaiymyxa ABP166,
In still anodier embodiment, the bacterial strain is selected from the grou consisting of: Badlhx amyloBq BBcism BS27, Bacl!iu* smyloBq ifac m BS2084, a illus amyhiiqiBB cm I5A1H B dBm umyioBqmfBck 3APd, BadBm iykBiquBack LSSAOI, ofo Bic >mBBiq fack^s ABP27S, BadB a yhBqcifaciem It) 13, Bacillus amykBiquifcKHm 91 S, Ba lha a ioBq ckns 22CFKand BacHim amykBqui/ ck BS I «> Bad lias BcBe formls &ΑΜΊ BaaB ch ifof is BL21 Bacillus subBlk 3 BBS, and B e b lBm palymyxa ABP166, and combinations thereof
Agieoh and Damsco USA !oc, of W227 N752 Westmooad Dr, Waukesha, A j 5318 , USA authorize DuPont Nutrition Biosciences S (formerly Dauseo A/S) of Langehrogade L PC) Box 17, D -1O0L, Copetihagen fC, Denmark to refer to these deposited biological materials in this patent application and have given unreserved and irrevocable consent to die deposited materia! being made available to the public,
A, S iM tyioiiquifack
The inventors ha e A and av.n one or more pams of Bacillus amyioiiqmk iercs have activity against p sst pathogens, including harmful bacteria, fungi and nematodes. The inventors have also found that one or more strains of Bacillus amyioBquilack sun improve plan performance. The inventors have also found that compositions comprising one or more strains of Baeil!m amyioiiqiB/uek are active against plant pathogens, including harmful bacteria, fungi and ^nemaodes and that compositions comprising one or more strains can increase plant performance. The Inventors have found that B iluA
Figure imgf000014_0001
BSs 7 has aebvhy agans plant pathogens, inclnding harmful bacteria, fungi and neinafodes- I bo inventors ha- ·. v abo f und that ,¾¾¾S a;y 00{iibotoiiy BSs / can improve? g!aai perfoonariee. fisc mvemors have als f un that coniposbiona comprising Bc&kii s
Figure imgf000014_0002
are active against plant pahsogens, brehidirig harmful bacteria, fursgs and nematodes and that compositions -oorgva-ong the strain can improve plant periommrice.
In eeram embodiments, any deovaine or variant of adttus oambo ;o?isr/,aia S77 l« iootnded and a- ovofid in the com sitions and methods, described and claimed herein.
Irs a other «mbcdftn$ftf, a strain having ail the identifying characteristics otdboobbo
myhki ui faciei BS27 is nserib in the compositions and methods described and clamied heren,
•fa.. g.-;.,- ,.- ·' '-■,·'· λ .>y h
Figure imgf000014_0003
Ser ice Culture C llection iNRRL), f 81 rt University Smcc;. be ria, id,, 6 ! 604 and given accession number NRRL B-50105. The deposits ¾w mute imdet the provisions of Ode Budapest Treaty on the internat nal Recognition of the Deposit of Microorganisms for the Purposes of Patent box' -.-boo:
λ BacMas amyidiqmf BS2084
live inventors have found thai Bacillus mydiq aci BS20I has aetieny agsmsi piani pathogens, mcludlog haoobb bacteria, tomy and nematodes. The inventors have aiso fm.tnd dao rioed/as myio!kjxi/ackns BS2084 ;¾n improve, nmo perforr«anco. Thsa in estors have also found thai compositions comprising Badiius a yhhqatfydem 882084 are isefvve against plant pathogens, ineioding harmful actei , fungi and nematodes and that compositions comprising the strain can improve plant performance.
In certain e«dodiments, any deri ative or variant of Badt'iits o y ik nfa kr^ BS2084 s included anci is osefnl in di compositions and methods described and claimed herem,
in soother embodiment, a strain having all the identifying characteristics of Badil
xmyioi Bf ckm fiSsOgd is ussfn! bi the compositions and methods described and claimed herein,
Ba ikiS miyhfiquif e ns BS20S4 was deposited on Mar.8, 2007 at the Agricultural Research Servlee Culture Collection (NRRL), 131 Norih University Street, Peoria, lib, 6160 and given accession number NRRL B--500] s. The deposits were made under the provisions of the Budapest Treaty on the international Recognition of the Deposit of Microorganisms for die Pur les of Patent Procedure.
J. B iUus aniykdiqdfhde 1 SAP4
The inventors have found hai Ba ik yb:ki uift.; k?N. 15AP4 has activity agai st plant pathogens, moindmg harmful bacteria, ku i and nematodes. The hwenrors have also fomm that ο· !,.:-Ί■>.-.· o;gi¾j//g¾¾¾:/of?s 15AP4 ears ireprov« pan performance. The seniors have also found that compositions co prishsg Ba -fins
Figure imgf000015_0001
I5 P4 are active against pla t ath ge s, including harmful bacteria, fungi and nematodes and that wmposit ss comprising the str in ear; improve plant pcrtormarioe.
is certain eo'ebodlmenis, any deri ative or variant -A ore e yioim ifkek'iis le tO h included and Is useful in t¼ compositions and methods described and claimed heren.
fa another embodiment, a strain havng all the identifying characteristics of Budilns
mtylotiqtdfius s ISAP4 is usefbt m the com posiio s m4 methods described and cianiied herein.
On km.12, 200$, Sadttm m ioiiq fiKim I5AP4 was deposited at the American Type Culture Collection tATCC}, !OSO? University Blvd.. Manassas, Va, 20 Π 0 -2209 and given secessi n numbers PTA-6507. The deposit was made under the provisions ef the Budapest Treaty on the iniernafenaJ Recognition of the Deposit of Microomanisnvs for She Purposes of Patent Procedure.
A Baeitim emyMi it!faefem 3 AIM
The inventofs have fonnd thai BacUim amyiosiq lfack 3 P4 has activity ag inst pant pathogens, including harmtwl bacteria, fungi and nematodes. The inventors have also found that Baa'itw wvioiddf c ns 3AP4 cars improve plant erf rmance. The Inventors have also fonnd thut compositions comprising i¾¾r;/te amyioli ifixie^s 3AP4 ¾re active against plant pathogens, including, harmful bacteria, fungi and nematodes and that compositions comprising the strain ca» improve plant erfor a ce.
hi certain em diments, any derivative or vsriaot of BadJhi<> amyfoiiq iaekm 3A.F4 is also included and is useful in the compositions and methods described arid churned herein..
In another embodd-oem, a strain having all the ktemirying c aracte istics of Brtcrfi
t nicii nf ckm 3 AF is useful in the compositions and methods described and claimed herem.
On Jan.12, dbos. Baefils amy ii jhefms 3AP4 was deposited at the American Type Carfare Collection (ATCCa lOAOi University Blvd., Manassas, Va, 20110-2209 and given accession monbers PTAmSOb, The deposit was made under the provisions of Che Budapest Treaty on the huerna onal Recognition of the Deposit of Microorganisms or the Purposes of Patent Procedure.
A Baelttm amyieUq i/mkm LSSAOI
The inventors have found that B dtfm amykiiqi-dciem LSSAOI has activity sgajnsr plant atho e s, including harmful bacteria, fungi and nematodes. The inventors have also found x\m B cill
Figure imgf000015_0002
LSSA I can improve plant performance. The inventors have also found that compositions eornpmmg one or m e strains of B d-Hu a yhiiq hckm LSSAOI are active against plant atho ens, including harmful bacteria, fungi and nematodes and thai compositons comprising the strain can improve plant performance. In certain embodi ents, any derivative or variant t Baetfi'm cimykBkwfite. m LSSAO! is included and is useful m die -:-:--^' -B; s and mei ods described and eiaimed herein.
la another ernbodhnenh a strai avin all ¾ e identifying cl acterisdos of Bacillus
a yioBq ifaciesi LSSAOUs ir f in the compositions and methods described and claimed InAein.
BasiBw; amylo!iqmfix em LSSAOl was deposited Jan, 22s 2O08 at the Agrkarkural Research Service Culture Collection (NRRLy 1815 " orth University St eet, Peoria III,, 6)604 and gi en accession number NR L -5 104. The deposits we e ma.de under the rovisions of the Budapest Treaty on the bPernanrand Recognition of the Deposit of Siicroorgardsms for the Purposes of Paten; Procedure.
4 /Poad'io- mnyk>Uq ifa i s ABPtJ
The inventors have found thsiBmUlm cimyiohm csei-ss ABR278 h s aeuvltv against plans pathogens, moludhrg harmful bacteria, fungi and ema odes. inventors have also found thai BociB im oo«a(aa ;e«v ABP278 can improve plant performance The inv ntor? nave also found thai compositions comprising .B cili Qmyfoliquifacrexs ABP2?s are active uao-oo plant pathogens, including ha mful bacteria, fungi arid mamttodes and that compositions comprising the strain c*w> improve plant perArroiUies.
In certain en siiment , any derivative or variant, of BacBha a kiigui/acktis ABF 8 is included and is u eful in the comp sition and methods described and clamed herein,
in a d'sCT embo iment, a strain having ail the identitying characteristics of clii
:ν>ο··ν· ;·::θΑ?:·/ν;ο ABF27o is useful in the compositions and methods described and claimed herein.
Bacillus c ybUq iiacsen A& 2H wa deposned by Andy adrsen of 227 7.5 A -.eon-aav; Or. Waukesha. Wi 3186, USA for Damsco USA Inc. of W227 NAA7 Weatmotmd Dr., Waukesha, Wi 55186, USA under the Budapest Treaty at the Agricultural Research Ser ice Culture Collection f'NRRL) at 1815 Nonh University Street. Peoria., Hi bo is 6 ί 6 ,. United States of America, under deposit number R.RL B-S cAa on 9 January 20 i 2. The deposits; were inn.de under the provisions of the Budapest Treat on d)e international Recognition of the Deposit of Microorganisms for the Purposes of Potent Procedure
The inventors have found xhttBtMnHm amylGiiqitmciens 1013 has activity agamst plant pathogens, including harmful bacteria, rungs and riemasodes. The inventors have also found that &¾o!!«s
Figure imgf000016_0001
10] can improve plant performance. The inventors have also found that
eontpositions comprsing Jft?ci//oo ose!.¾ooey¾ioe;¾ 1015 are active against plant pathogens, including harmful bacteria, fungi and nematodes and that compositions corn prising the strain can improve plant performance.
in certain embodiments, any derivative or■ :>·">ηο of teuore a ykUquifac s 1013 is ineirafed and is useful in the compositio s and mehods described and clamed herein. in anoSher etnbodimtmt, a st afe havmg all the identifying characteristics otBaciUm amyh i dhekfss 1 53 is useful in the compositions and methods described ami claimed herein.
Bacillus mbtM? JOB as deposited at the Agricultural Research Service Cuiiore Collection (NR.RL) at i 8 i ? North IhV ersity Srseet, Peoria, Itlmors 6 ! 604, I .hiiied Sisses of America on May 1 X 2011 and given accession number NR L 8-S0509- The deposits were mrrde under rise pro isions of the Budapest Treaty on the International ecogntion of the Deposit of Mkro rgan sms for the P rposes of Patera Proeednre,
?t Baeiffus iviafiquifke 91
The inventors have found that /o dgo .» ioi;quffaci& 918 has activity against plai , pathogens, including harmful bacteria, fungi and nematodes. The invenrors have ab found thai BaetU ro'fnaoa7i/do/oss 91 $ can i pre-ve plant performs nee. The inventors To e also found d-.o composlnoiis comprising /to- a/to o y!ohf ihcwt-ix 918 ssre acti e agsmst plant pathogens, including hannrid bacteria fungi and nematodes and that compositions comprising the strain esan improve lans erf nce.
In certain embodiments, any derivative or variant of i¾s-:h/u«
Figure imgf000017_0001
91 S is also included and is useful in she com ositions and methods described and claimed herein,
'in another embed imeni, a soain having ad t e moohfy me eharaetcmiies of Baciih^
uniyfoiiqui/atiii 91 S is rsseful in live compositions and methods described red claimed herein.
Baciiiiis amyl iiqtfij-cf ns 918 was deposited at the Agrieu!mrai 'Research Service Cuhnre Collection (NRRL) at 18IS North University Street, Peoria, !jfmois 61604, United States of America on May 13, 20 ) 1 and given aceossson number N'RRL B-5050S, The deposits o vre nosd.- under the provisions of the Budapest Treaty oa rise international Recognition of the Deposit of leKKS'Saibss for the Purposes of Parent Procedure,
ip W& myieU uifit-ctem 22€M
The inventors have fom that Bacili oia-/o2g o/o-;2so 22c T7 has activity against plant pathogens, including harmful bacteria, fungi and nematodes. The inventors have also found that BwMhm a yioHq ui/ aen^ 22CPI can Improve plant performance. The inventors have also round that com postions comprising Baciii cmndoHqidf iens 22CPI are active against plant pathogens, including harmful bacteria, fungi and nematodes and that compositions comprising the strain can improve plant performance,
la ssitsh; embodiments, ens derivative or variani otBi&iUm mnyhiiq ifhcimv 2.:\ Pi is included and the compositions and methods described and claimed herein.
in another embodiment, a strain having ail she identifying characteristics of Bacilfes a yhiiQiiifyckns 22CPI is useful m the compositi ns sod methods described and claimed herein On ten. 1 A 2005, ik 'ihtv
Figure imgf000018_0001
eyfofA s depoaked si s Ameman T pe Culture Collection ;ATCC), iOSOi Universit Blvd., Manassas, Vs.20110-2209 and given accession number PTA-6501 220 " !). The deposits were made tmder the provisions of the Budapest Treaty on the Internationa! Reoognidon of the Deposit of Mreroorga.nismsd r the Ρ·οροο·> of Patent "o- odnro
m Ba U!m myteliqidfadem BSl$
The inventors have found that B^ciil amyioBq-uif a ts B S8 has activity against piarP pathogens, including harmful bacteria, Pmgs and nematodes. The inventors have aUo found that Ikidiin- «»n oo/npgio0;¾.¥ B Ss can uis rove plan* pe fonnaoee. The inventors have also hound dsat compositions oon i aag acUi-m
Figure imgf000018_0002
!fo ) x are active against plant pathogens, inchming harmful bacteria, fungi and η«ηι¾ίκ!«8 and T ai com ositi ns comprising de strain cars improve plant P'-oiooneno.'
in coitftha embc ifoieats, any derivative or- vananteTA vV¾ mnyiokquBBekns S\ is ho- -Toed and is useful in the compositions and methods described and claime herein.
in another embodiment, a strain a ing ad die identpysng o araciensfios of /Aav/for
m k'liqiqfy BS ί S is useful in the compositions and sned'iods described and ckbused hecem.
AVe ·;^" amy iiymfac m BS IS was deposited by Andy fvkdisen of W22" 752 WeoPnound Dr. Waukesha, Wi 53)86, USA for Dan eo USA Inc. of W22? N7S2 Westroound Dr. Waakeshn, Wi 53186, USA under the Budapest Treaty at the Agri yfoura. Research Service Culture Collection (NKRf .) at IS15 North t o>o eo n\ Street, Peoria, Illinois 61604, United States of America, under deposit numbers N 'RL B-S0P33 on 9 January 2012.
g, B M s Hekm^m
The inventors hav¾ 'found tha one or more sPsins of ·■· · Am.-, Pcieag%>r¾fo have activity sgamsi plant pathogens, drclodiug harmfiii bacteria, fungi and nematodes, fhe in entors have also found that one or more strains of Boc/foot Bck ifor is can im r e plant performance. The inventors oc also found that com ositons comprising one or sn re strains fM if!us P' vegpovoo" are active agaimd plant pathogens, including hnraful bacteria, fungi and nematodes and thai compositions comprising the strain nan improve plant performance,
A Mii im iiehenifi nk BAM42
'the invontoo; have f.and that B ciiius iichekfyrmk BAST2 has activity against plant pathogens, including harmful haei&ri. utigi and u&jnatodes. Use weniors have also found that Bacillus
Ueheniformis ΕΛΜ2 cars improve plant pefbrmtmee. The inventors have also found that compositions ■,'o;a:a vdag Bacili ik e kbr k BASd2 are aeli e against plant pathogens,. In luding harmful bacteria, fungi and nematodes and that compositions comprising the strain can improve plaes perfbttnance. i certain v a< dam:su> ¾;sy deH aUvc or variant of Ar?cA;A? vs-o;a.yoo BAadg Is ne.dm: A and A a.oTn « da compositions and methods described and claimed herei
in another embodimen , a srain having all the klendfyirrg: characteristics of Baciiiw !ichenifyrm!s BA Z is useful in ids compositions and meth ds d scribed ;·■;·· a claimed herein.
B cillus iic cnifbrtfsis BAfoi2vvSs de sied at the Agriefolnrai ese c Service Culture Collection (NRRL) at 1815 North University Street, fy ··:··!··. Illinois 6)60-1 United Smtes of America on May 20, 201 ] and given accession number NRRL B-.50S 1 d. All of these deposits were made under the provisions of the Budapest Treaty on the International Recognition of the Deposit of icr orga is s for the Purposes of Patent Procedure.
2 ciU Mc if&rmis B I
The irrsentors have found thai Bacillus tiahedfor Hi id has activity against plant pathogens, including imrmtoi bacteria, fungi and nemat des. The mvenUas have also found that Baciir
Ae/sra/Ae-scA B.L21 can improve plant perf rm^nee. The inventors; have also found that coinposksom comprising Baciiiw; iic nn rmis 8L 1 are a t e against plant pat ogens, including harmful bacteria, fangs and nemato es sad that compositions comprising the strain can improve plant performance.
In certain embo iments, say derivative or variant■■>' A.-Ab;.v ikki>?nibr!>us BL2! is also included and is useful i the compositions and methods described and claimed herein.
In another embodiment, a strain having all die identifying cfmraotensties of Bacii!ii* li henuhr is BL2I is useful in ihe coinpositions and methods described and claimed herein,
AavAus c/icad' fSfsd Hi. i i vat; deposited at Ihe Agneuluuvd Research Service Ctdture
Collection ( RL) at 181 a North University Street Peoria, Illinois 61604. Unhed States of America on Apr.15, 2008 and given accession number RRL B-50 4, AH of these deposits were made under the provisions of the Budapest T reaty on the international Recognition of the Deposit of Microorgan sms for the Fur osicc of Patent Procedures
BaeillM snbtilb
'The inventors have found that one or more strains of Aoc///¾s aafo/IA have aePAy against plant pathogens, imduding harm hb bacteria., ;o .a and nematodes. The inventors .have also found diet one or more strains f Aradfo vrAidA can improve plant performa ce. The inventors have also found da s. compositions comprising one or more train^ of BudJius savdA
are active against plant pathogens, including harmful bacteria, f ttgi arid nematodes and that compositions comprising the strain can improve plant performance.
i. Aocd/Av bt h sBBS
The in e rs have found ace Bacillus mbtiiis : A :: has activity agamsi plant pathogens, including harmf I bacteria, fungi and nematodes, The inventors 'have also found that Baciiiw s btfiis J0 can impmvsf plant performance. The Investors have also found t i com ositons comprising Bi&dfi s snbPHs iA 5 are active against plant pathogens, melading hattofui bacteria, fungi sad nematodes: gad beat coo'tposnions eoospnsirng. the strain can im rov plant' perfsrmanoc.
in cci in embodimenty an e ivat e or variant of B<?e!tf.Hs ub s 33P5 as also inclu ed and as useful lo the compositions and meth ds described and eiaimed herein.
In another embod invent, a strain having ail h identifying characteristics of dihis suhti .$ 3BP5 is useful in the compositions and methods described and etaiated heroin.
A cbA; snbo/A 3BF5 was deposited by Daolsoo USA, inc. of AAoheahc Wis..n ?nv Agricultural Research Service Culture Collection ("N' RL);, 18 i S North University Sn-eet, Peoria, lb .6160 on May 13,2011 and assi ned 'RRL B-50510, AH of the deposits were made under Ova provisions of the Budapest Treaty n the Iniernatsorras Recognition of the Deposit of Microorganisms for bse Pta oses of Patent broeedvao.
The inventors have found that one or more strains of P enibsi'ii a paiy yxa have activity against plant pathogens, including .harmful bacteria, fungi and nematodes. The inventors have also found that one or more stnnns of Pnefifba iikis p ly yxa can improve plant performance. The ins amors have also found Ova compositions c m osing one or more strains of .fba:on bacp/ae cyjagrvo are co e against plant pathogens, inckrdlng hanrbvd bacteria, fongi and nematode*; and that compositions comprising the strain can improve plant performance.
A Ai ikteifim p fymyx A P m
The inventors reive found thiU «Ι Ate/ a.v po m-χ AB I 66 has activity against plant pathogens, indudmg harmful ba teria, fungi and nem todes. The inventors have also found that
Pae ibaciiius poi )v?rysw A F166 am improve plant performance. The inventors have also found that compositions comprising one Of more strains of ' Pxe?nbticfik& pabymy ABfobtS are active agauvst plant pathogens, A:hkbsg harmful bacteria, fungi and nematodes and oca eomposUKms eomprisinp the strain catt approve plant performanee.
In certain embo iments, any derivative or variant of P ntb&cltim polymy ABF 166 is also included and is useful in the compositions and methods described and claimed herein,
In another embodimem, a strain having ail die idernilying characteristics of Pavnibacitii
poiymyxa ABFI 66 is useful In tbe compositions and methods described and claimed herein.
Paenib ciH-ii&■ polymyxci strain ·\ΒΡΑ·Ά was deposited by Danisco USA. Inc. of Waukesha, Wisconsin at the Agneuli a! Research Service Culture Collection (IbR L , 1 SI5 fsortb University Street, Peoria. IH,, 61604 or; December IS.2008 under accession number B-S9211. The deposit was made under the po isi ns of rhe udapest dreary on the hnernntional t^eP nbon of the s.Wrt n ··; Mic oorganisms for -he Purposes of b nets Procedure.
Panisoo USA hso of W227 N752 Westmound Dr, Waukesha, v.1 S3 ISO, USA authorize DuPont Nutrition Biosciences S (formerl Darnsco A/S) of Langebrogade i, PO Bos 17, DK--100),
Cope hagen , Denmark to refer to these eposited biological materials n this; patent application and have given unreserved and irrevocable consent to the deposited -maoriA being made available to the public,
E, €«li«»¾f the teais*
s he niepdum used to cultivate an /or grow the strains disclosed herein may be any conventional medium suitab'e tor er wmg bacie-rlgl strains. Bacterial attains disclosed herein ma be grown under any conditions suitable i r growth.
The catering can take place with, on. or In the presence of one or more subsrates (e.g. n fermentable substrate), A fermentable substrate Is materia! that contains an organic compound such as a carbohydrate teat e r; be transformed (eye, convened imo another compound i try Ac eneyrnarie stetson of a bacterium as disclosed herein.
Examples of substrate include, but art; not limited to, non-fat dry milk, vegetables (e.g., com potatoes, cabbage), starch, gmlss fe.g., rice, wheat barley, hops),, fruit (e.g., grapes, apples, orsrrgeO. sugar, sugarcane, meat (e g., beef poultry, pork, sausage;, heart infusion, cultured dextrose, combinations thereof, and the like and suitable media containing proteins, carbohydrates, arid muterals necessary or optimal growth. A nor lm using exemplary medium is 'FSB- or CASO broth,
in one embodiment, the substrate may include one Of more of starch, soy. yeast extracts and salts, in set another embodiment, she growth medium may be CASO broh, in still another embodiment, the growth medium ma be Ϊ8Β broth,
The euh4rrlng of the bacterial strains disclosed herein can take place for any suitable time. In one embodiment, euHuring the bacterial strains cars take pla.ee for a period of time conducive to prodocc a compound of Interest. For e am le, the catering can take place from aboel S to about 72 hoars (h). from abom 5 to about 60 h, or from about 10 to about 54 h or from 24 to 8 h. In one aspect the ce!mnng cars suitably Oke place for about 1,2, 3, 4, 3.6, 7. κ py |o, 11, 12, 13.14, 15, 16, 17, A 19, 20, 21, 22.23, 24.25, 26, 27.2S529, 30, 3 L 32, 33, 34, 3S.36, 42, 48, 54, 60 h, where any of the staled values can form an upper or lower endpomt when appropriate. In another aspect, the rime for oohtrring can be greater than or equal m about i, 2: 3,4, 5, o ?, 8,9, 10, 11, 12, 13, 14, 15, 16, 17.18, 19.20, 21, 22, 23, 24, 25.26, 27, 28, 20.30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41 , 42, 43, 44.45, 46.47, 48, 40, 50, 51, 52, 53, 54, S5, 56, 57, 58559.60 h. In yet another aspect, the time for catering can be less titan or equal to about 1 , 2.3,4, 5,677, 8,6. Kg 11, 12, 13, 14, 15, 16, A. It, 19, 20, 21.22, 23,24,25, 26, 27,28, 20,30.31.52, S3, 34 35. 36, 7, 38, 59, J L 42. 43, 44, 45, 40 7, 48, 9, 50. 51 , 77. 5 , 54. SS, 56, S3, 58, 5 , and 60 h, Sn si ill another as ect the euhariug occurs for i5ppn» m¾tei.y 24 ?o 48 hours
hi yet anobrer embodiment, she yahnnng occurs for approximately 20 a - 30 hoars In another embodimen da- eabnnng can be carried oui umil nutrient depletion occurs,
la one fm diroenL the eulturing is o a tun« «fi¾cUv© io reach th& sta aary phase of growth of the bacteria. Use temperature daring the euiiuring can be from about 30 ro about 55aC from about 25 to sboal 40 %2, or from about 30 to about 35 2 In ne as ect the temperature during the ouhur g -an be iron) about 20 0:; ahead WC from about 30 to about 4irC2 or from about 40 to about 50%2
iu another enmodimeni, the enkuri g arm take piece a?: a temperature of about 20, 21 , 22, 23, 24, 25. 2b, 22, 28. 20, 30. 31 , 32, 33, 34, 35, 3d, 52, 38, 39, 40, 4 L 42, 43, 44, 45, 402 4?, 48, 49, 50. 5 h 52, 53, 54, r 55%2 where any of dee stated values can form an upper or lower imdpoint when appropriate. ?n still anofber ernbodimenh the eulturing can take place at a semperalare greater than or equal io about, 20, 1 , 22, 23, 24 , 22. 26, 27, 28, 22. 30, 3 i , 32, 33, 34, 55. 36, 37, 38, 30, 30 41. 42, 43, 44, 45. 4b, 47, 48, 4b, 50, 51 , 52, 53, 54, or 55· C. iu yei another aspect, the euJtaring can take place at a temperature Daa than or equal to about 20, 21 , 22, .23. 27 25, 20, 22, 25. 20, 30, 3 L 32, 35. %5 35, 36, 32. 3a, 39, 40, 4 b 42. 43, 44. 45, 56, 42, 41, 49, 50, 5 i , 52, 53, 55. or 55°C.
iu one embodiment, the eulturmg can occur from about 30 to about 3SaC. In a further aspect, the ceHuring can occur at about 327 2
in yet another embod me t, she cidtormg preferably may take place under aeration le oue embodiment, the level of the aeration is controlled. Aeration levels may be expressed as dissolved oxygen tension (POT), w some 5; DOT is a percentage of oxygen saturation in the culture, fog. ! 0P¾¾ DOT means a cul ure is fui!y saturated w th oxygen). .DOT may be measured as taught &srosn er αί.
' echni ues for oxy en transfer measurement in bior &ciofs: review" 5 Cha Techno! Bioieehnol 2000; 84 ; 1 9 P i i 03 (and references therein), which is incorporated herein by reference, or as taught n abey 37 Bailey .f, O!iis D, "Biochendcal Engineering Fanigm UaaiO. 2s* eddioa, bdcCOa -hbli, ISBN 002003 122 (and references therein g which is ioeorporated herein by rdir¾io«.
In one en hods mem, cutiuriog does not take place under conditions at which oxygen content is litrbbng. in another enrbodiment, the le el of the aeration, is such that the oxygen content io the culture is more bum about 200, DOT, more than about 30% DOT, more hat! abo«M0% DOT, more than about 50% DOT, more than about 60% DOT, more t m shout 7055 DOT, more than about 8055 DOT or mo e than about 9055 DOT, In some aapects, the level of the aeration in die -uhmro is abom ! OOob DOT-
In still another embodiment, da; level of aeration is such that the oxygen con ent its the euhure may be between about 255b and 505a DOT.
The aeration may he pros idee b any suitable raedu -a. In mm® mibodlmmn> ti m i «a«y by profited fey any m«&ns th&i mhm with the mfom. Thus, the aeration naay b provided by agitation (e.g. shaking, oscillation, stimng etc, } or by passing air (eg, oxygen) t rou the culture m&dls, for example, or eo b ation thereof.
The rate of aeration expressed as vv (the volume of gas per liqu d volume per minute) may fee measured as taught In Bailey J, Bailey Jf« OHis D, "Biochemical Engineering FendamemahT 2"° edition, McGraw-Hill, ISBN 9070032122 (and references dtereinf which is incorporated herein by reference, for ex. am pie.
In one embodiment the aemtioo rate mm be Is the range of abend 0, 1 to about 0 vent. Where the aeration k provided by agitation (e.g. in a stirred ierrneoror) then the aeration r te may be in the range of about Cb l to about 3 vvm. Where the aeration is provided by passing air dtroagh the culture media (e.g. In an airlift iermenlor) then the aeration rate may be In the range of about 3 to about 6 vvm.
In ctbi another embroilment, a culture container that is desi ed or shaped to support or provide aeration may be used. In one embodiment, the culture container may comprise one or more bailies, The aim of the bailies may be to encourage ex osure of the media to oxygen (cyy air}, For example, a culture container with baffles may be used in combination with shaking or oscillation of the culture container. B way of example, the culture container may be the container described in V.S. Patent Mo, 7.381 ,559 (the subject matter of which is incorporated herein by reference).
In another embodiment, the culture mediant rosy be agitated. This ntay be alleoted by amy conventional means. Without wishing to be bound by theory, agitation of a eoiture medium may have a number of beneficial effects when compared to a non-agitated culture medium, including but not limited to: increased growth and/or decreased cell clumping and/or increased nutrient (e.g. carbohydrate) mixmg and/or tetter nutrient distnbntkm aud/o? Increased protein production and/or increased primary metabolite production and/or Increased secondary metabolite production etc. In one aspect, the beneficial effects derived from agitating a culture medium may result from the creation of turbulence withi the cubore medium bet-', by stirring), in one emtedimeog the agitation may be stirring. In another embodiment the agitation may be shaking or oscillation.
In one aspect the culture media is agitated by oscillation (e.g. by rotatory shaking), in another embodiment, die speed of rotation tmy be at about 5b to about 250 rpm, about 60:epro to about 240 rpm, about 7b rpm to about 230 rpm, about 80 rpm to about 220 rpm, about SO rpm to bo t 210 rpm. or about 00 rpm to about 200 rpm,
Its still another embodiment, dtc- speed of rotation may be at about 100 rpm to about 1 50 rpm. Suitably the speed of rotation may be at about 1 0 rpm.
In one embodiment, tire culture medium is agitated in order to Increase the level of aeration in the culture media and/or increase nutrient mixing in the culture media. It has bees found thai aeration and or agit&iidr* of the culture mixture may result in significant im r ve ents in the iermentase produced . Without wishing to be bouttd by theory, da:- invpowv ent may be paused by ensuring the ceil denrity or cell mass in the outers container is such that the protein yield and/or primary metabolite production by the bacteria is enhance in the fermemai¾.
I« cm aspect, the eu!mre media may be agitated by stirring. The speed of stirring may sui ably be greater thai! about SO rgm, for example between shorn SO rpm to about 1200 yarn The rate at which the culture media may be stirred may be dependent upon the container in which it is held for caterin pu poses if the eoat&iner comprising the culture media is a small fermentor (s.g. less i 500 L, such as aboot 100 to abof.it 500 L or even less thar! 20 L). then the speed of smring may be at kmw about i OO rpm io about 12 rprm tor example. In some aspects, the speed of rtirrmg may bo greater bom about I 200 rpm, if the container com rising the eohure media is an industrial scale fermentor (yog, greater than 500!.,, such as about 500 to afoo 20,000 I,)., then the speed of stirring may be a least about 50 rpm to abou 150 rpm or may be greater than about 150 rpm, for example,
in an ther aspect, agitation of a culture media during entering may be iv/preseoted as po e input by agitation, ?·· a -r input by agitation w a sepresentation of the amount of energy prov ided per Oier of hqaid volume. The power ingot by agsPdion cars be calculated by fOss determining the power in awyoa using the follwvjog tonrnda:
A «= N n*
where M6 is a dimensioo!ess number /Newton number); p is the density of the liquid (kg/nr); N (s") is tite rotational frequency and D is the impeller diameter (m). Pt. k the power drawn by an agitator when the culture is not aersted. O-uwuabwi of p wer hq>m by aoawts -n in the presence of aeration is iaagh? ia Ohnos et o/. 'w'treots of bioneaetor hydrodynamics on the physiology oi S ynom c s", Bioprocess Biosysl Eng, 20 i 2 Aug OS arid references thereby which is incorporated herein by refeteaee,
la one aspeey during euiturbvg: the power ingot by agitation per volume may be at least about 0,25 fcW/nr . in aoother embodiment power input by agitation per volume may be in the range of about 0.25 kW/nr to about 6 RW/mO fn another aspect, the power input by agitation per volume may be In the range of about 0.25 h o. Sub m about a kVWnr\
Io another aspect, the culture volume to the container volume may be less than about 1 : 1 v/'v, e,yy 1 :2, i :3, etc. In some aspects, the ratio of the endure volume to rhe container volume may be less than about i : l v/v, 1 :2 v/'v, 1 :2 v/vy Ϊ Ά v/v, ) v/y 1 :6 v/v, 1 ;7 YW 1 : 8 V/V, i :0 v/v, or 1 ; 10 v/v.
in some aspects, the ratio of the culture volume to the container volume may be in the range of about i oi v/v to about 1 : 10 v/v, so one aspect, io the raoge of 1 .3 v/v to about 1 : / v/v.
In some aspects, the ratio of the culture volume to the container volume may be about 1 : 1 v/v, 1 :2 v/v, 1 :3 v/v, 1 :4 v/v, 1 :5 v/v, I ;6 v/v, i 2 vwy 1 :8 v/v, 1 :0 v/v or 1 Ί 0 v/v. in a»0ih©r em odinrsnt the ratio of the culture volume to she container > au c may he -boo; ! :5 v v.
05 one ^m lme b the ernone of cohufe ntay bo k¾s than about 1 6o¾, loss unm about 90%. less than about 80%, loss than about 70%. less than about 60%, boss dam about 50 , loss than about 40% or os than about 3 % that of the container volume,
in another embodiment, the volume of the culture may bo in the range of about 60% to about 90% that of the container volume, for example.
In stfb soother embodiment, the volume of the .. o!mre may e in the raoge of aboul 70% k; about 85% Ova of the container olume.
Tbo phi during the enlisting, can bo at a pU f»i« about J to aboai 9, from about 5 to about 6, from about 6 to about 7, from about 7 to about 8, or ftom about % to about 9. In another aspect, the eulturiog ears take place at a pH of about S, 6, 7, 8, 9. where any of tbo stated alues can form ars upper or lower endpomt when appropriate, in one aspcci, the pH Is at a pH between about 7 and about S, from about 7 to about 7.5, irom aboib 7.1 to about 7.3 during the cultming. In one aspect, the euiturmg is at about pH 7.3 ,
H another embodiment, the pH ma be adjusted after cu!turing to a pH from about 6 to about 0, or from about S to about 10, or from about 9 to 10. bs still another erob-odlmem, the t may be adjusted '■'■·■ -: about pfl 8 as a ut pH 9, n; yet another embodiment, ihe pl i may be adjus ed to about pH :>.
hi one embodiment an alkali may be used to increase the pH. in one embodiment, otassium hydroxide (KOH) may be used ,
In yet anothe ensbodimenh the pH Is adjusted aber separation of the bacterad cells and culture media ( .g. by eenrriiugation}. In one embodiment, ho;- pf l of the supernatant is adjusted.
In another em d ment:, the eu!tarmg step c m rises one or more adjustments of the cult ure conditions isueh as an adjustment of pl . temperature a d/ or substrate) during the eabunng phase.
Without wishing to be bound by theory, adjusting t e culture eondhions io.p, pfl. temperature and/or substrate) during the euitursng may increase the number of compounds of interest produced during the culturi g process. For exatnple, the initial culture con itions may be conducive to produce one compound of Inter es and the adjustment of the cultur conditions may provide favorable conditions to produce a f i!ter eouipoimd of interest
"T hus, tor example, urin the culturbsg process an initial pH of about pH S may produce one compound of interest. Subsequent adjustment cf the pH to pH 7 during the same cu I luring process may result m the production of further compound of mprest.
Batch and c n inuous cuburmg are k ow to person of ordinar skill in the art. A fr rnent oon product of the disclosure or a portion thereof comprising eoR>potmd(s) of interest may be prepared using batch or coodsusous cbtur pg. Suitably, the ffermeft ton j>f©^««i or & t n thereof oiay bo harvested during o at iho end of the culturmg process
In sod another embodiment, a iemtiint&noe product of the do«,.:o:oo is harvested dnriae or at the end of the exponential phase, In ne aspect, the fsrmers ksn product ofthe present invention is harvested or dining the stationary phase.
In anoher embodiment, the fermeaoirion product may be produced in a vat under commercial condlliooss.
In a si>i limiting escprnple, bMtoHai annas dlsaiosed heosan re o hnrod to between about a I a }(? CFU/ro! i about a. I x 10"' CPU/mi. The bacterial strains disclosed heieht can be grown in Tryptic Soy Broth (TS.B), Milk Based Media, or Optiivdeed industrial Media do Man, R gosa ri Shsrpc (MRS) broth at 32i¾C for 24 hours. The bacteria can be harvested by aermafoaailoa, and Pe snpernateist removed. in ooe embodiment, t e hoPorna- relates to composition comprising Of eorubstnus of or eomisPng e^semiaily ot one or more bacteria! series In one erobo innnp the composition comprises, consists oP or consists essentially Aba; · or more ofbseienai strains selected !torn the group aoesisting of: FJa>ciihis Oiop/ popac/efte BS27, Baalim
Figure imgf000026_0001
BS20 . Itaciii amyiaaqaqackm 1 AP4, B ciiiw aciyiaikpsifackns '■■
Figure imgf000026_0002
ABP27S, Bacillus a yloi!qiafac!e KB 3. Baciihts amyiaiiquqac m 91Z, Bacillus amkk!qikfackm 22CPK Ba ;!;;■:: amyioi!qik/ack BS18. A a Ba ikk nocmk BAPA2, A. :οΑΑ.? tkhcnif nnls B!Al, Aoooa spbi ls eAAs'. md Paenit cliius yolymyxa ABF) 66. la one emb dimen, the compostions are biologically pate cultures of the strains disclosed herein,
la apothea etobodlaaeog the disclosure relates to a composition eomprlAog at least two baeteHal strains; selected the group consisting of; Bacili&s amy isquifack BS 'a AaciMo?
amyfoiiqidfazk' BS20S4. Bniii!im am hii if ims } SB.BA AuoaV .··■ myfoilm/ifach 3 AP4, Baciiim cmiohkiiifhc 1. AS A Of Aor:lft½ a ykBqulfackm ΛΡΡΑΑ Bx iihis im oikpBfix- ns i i 3.
a ifhis amylaiiquBacie^s I 8, A a Abo oe -AAtpabAeA.e- 22CP 1 , .B :> - A a aaqBa'iqu A; ;--a> BS ! A BxcM/us iic mkhnnis iaPs A Bacillus lichcnim-mis BL 1 , Aoe/bAy :>ubiilis P5. aad P e;u baciiius ρϋίγ ψχα ASF 1 oa.
in still yet another embodiment, she disclosure relates to a composition comprising at least three bacterial strains selected from the gro >. u a t < T L
tOiipo ipp:a-A;s BbAOAA ArariAaa miyioiiquifis iws Ι5ΑΡ4, Baciu ospAoApMPaPens 3 AT' 4, BticUtw a yhkqulfackn I.SSAOT Aas/Ao remA> a bAeroi ΛΙ -B3"st Bacillus oop;.A/:o.;;.a vav- |oi P Baci!h oo CoApsPpis ess 918, ¾ο/ οί wm&Uq ifack 33b PI B eiites a ykB1quBacic»s BSI8, poixmyxa ABP!bfn
hi stHI yet another endmdsrssnt, the omeaours relates Pa a e¾mpessbon comprising at lcs-a t'oor bacterial occur the group consisting of: boorno m B uifixiexs ES27. acsUus ■ yioiiq; f ci s BS2084. Bac/!!m amyioHqt hcie ! 5AF4, Bacillus atny iiq ii cie 3Ar 4, Bae i s my i /mfijcie LSSAOb
Figure imgf000027_0001
ABP/78, acMui w?ty liqx4faci i 1 13:
Baciih® atnykuiqi;i†bcmi>; 9\t Bncu! w liqidfackm 22CFI, adil s iyb!iq if ci ns BSI 8, liche for is BAB42, ί½ο//Α« Hck iformis BL21 , Ba Hhs .whtii!x 3BP5, and Pa&tibe lifcs poiytm a ABPI66-.
In one embodiment, t e di closure rdato its c mpositio comprising die bacterial strains dscosed herein and one or more com ounds or agents selected from the group consisting op
agcoclmmically active compounds, bloeofitroi agents, lipo-ehltoobgosaechadde compounds (LCOs), isoflavones, oamiazolines, insectlddal compounds, aaolopyrlmidinyi mimay polymeric compounds, ionic c m und, siibsniuied tbiophenes, substituted dithiines, fluopyjrarom,. ena inocarbonyi compounds, strigolactone compound, and d ith Bnc-tetraearboxi sdc compounds.
in yet another embodiment, the disclosur relates to the use of a first e-mnposjuon comprising >hc bacterial strains disclosed herons and a second composition comprising one or more compounds or agent; selected from he group consisting of agroehemically active compounds, biecoao-oi agems, iipo- ohiioobgosaeoharide oornpotuKb i!XOsp koflavones. quws¾3iines, msectickla! compound,
azoiopynniidlnylamine, polymeric compounds, ionic compound, substituted tbiophenes. substituted dnbilne nuopymtnm, enaminooarbonyi compounds, atngolaetone compound, and ditbiioo- ietr carboxiralde com ounds.
to one emb diment, the disclosure relaes to the use of a first com siio co rising o e or m e bacterial strains disclosed heroin ami one or more compounds or agents selected from th© group consising of agrochemioaliy active com unds, blooonirel agents, bpo--ohitooiigosacebande compounds (LCDs), isofiavones, qumaaobnes, inseetioida] compound, azolopynffik!iny mine, polymeric compounds, i nic compound, substiuted truophenes, snbsuttaed dithenes, ilnopyranmo ens-ninocsrbonyl compounds, strfgolactone compound, and dthino-tetra aitoxlmkle compounds, and a second composition comprising one or more compounds or agents selected from the group consisting of:
agroehemmaRy active compounds, bioconfro! agents, sipo-chiio !igosaccharide compounds (LCOs), isollavones. qsnna oiines, seoticidai com ound, teolopyrimWinylamine. polymeric compounds, ionic compound, substit ted tbiopbenes, substituted d ith sines. flnopyfamm. en&m i ivocarbonyl com ou ds, strigoiae>one compound, and dnhbne emea mmide compounds. f* <··>ν· embodiment, the ''combined" use of ai least one bacterial strain disclosed herein v th :u least one agfochen'hcally soiree compounds, biooontroi agents, lipommfcxdigosaccharlde compounds (LCOsg isoftovones, qonasodmc.-, inseeticidal compound, axolopyrimkiiny mine, polymeric compounds, ion c com ound, suhsbtuted *hk>ph¾«es, su stituted dithiines, fh:mpyramm. enandnoearbonyl ompounds, sirigoiactons c mpo nd, and dldbltxotetracarhoxim le com und* or Use PeutmerP according to the disclosure with the at least one bacterial strain and st least one compound referenced herein can be underst od as m g a physical mixture of the bacterial simSn and ih« vompoutHi.
in yet ano her em odiment, the comb ned use may also co sul of usin one or more bacteria] strains disclosed herein and at i ; ssi one compound referenced herein separately, in yet another eoibodhoeni, die bacterial strain and compound may be used ithin a suiTleiemly short time of one anodser so diss the desi;¾d effect can take place.
In oae embodiment, the compositions disclosed herein improve plant performance or plant vigor beyond the Improvement observed ith die active com ounds alone (synergism).
in one embodiment, the action of a eomposnion disclosed herein goes beyond the action of the achvs compounds present io the mixture alone (synergists).
In yet another m diment, ie joint, separate, or successive application - e' hv bacterial strains disclosed herein and one or more one agrochemieaby active compounds, bioeontrol agents, lipo- cmtooligosacehamle compounds (LCOs), isofla ones, qunvrsolhves, Insecncidai compound,
azoiopyrimidinylaoune, polymeric compounds, ionic compound, snbsduued rhiophetres, substituted dithiines, floopyrantm, enamiaocarbonyi conrps¾onds, sirigolawone eott ound, and dnhibvo- teimearboxlmlde com ounds ellosvs enhanced control of pa!hagnes, harmful pests, sacb as harmful fungi, mseots, or anirnal pests, compared to the control rates that arc possible with the individual eompoe-nds (synergist c mlvinres).
In still yet soother embodiment, the joint, separate, or successive application of the haeterlai strains disclosed herein and one or more one agmehemieaiiy active compounds, blcKmaPr ; agency lipo- ehlsooligosacoharide compounds (LCOs), is ikvones., qumazolines,, Inseeticrdai compounds,
axotepynmidlnykiTOiiCS, polymeric compounds, ionic compounds, substituted tblophenes, substituted dltlblneoe fluopyfa m, eosmtnocarboayl compounds, strlgolaetone compounds, and dilhilno- ebaearbosJrnide compounds provides enhanced plant health effects compared to $b« plam heakh effects that ate possible with the individual compounds (synergistic anxun, wherein Pie synergism is lant health synersgismy
A, Btoeoutrol Agents
In one embodiment, rhe disclosure re lams to a composition comprising one or more bacterial strains disclosed herein and one or more bioeontrol agents. As used herein, the term "bioeontrol agent" C! €A*) moHioAs ono or m re aoAA , frnagi or sastss, p? km>ss, i uses, enario> ttd-a no nemat deR, and boiamcal extrseiy or products roduced by tnieroorgsnisms including proteins or secondary niUefr dp.\ and ηηη>. aUuA that have one or both oi «. following characteristics ( : } InhAlA or reduces plant sfosBii n ;o l'or growth of pathogens iueloding but riot !imded to pathogenic fungi, bscieria, and nematodes, as well as arthropod pests such as Insects, arachnids., chiiopods. dipiopods, or thai inhibits plant infestation asKo i- growth of a comb iaiioa .; plaas pat og ns; (2) n spr es plan: performaace; (a) boproves plant vAkl; (a) m r es plsai vi r; and (5; improves lans health,
Figure imgf000029_0001
In one s o hoosi: t e 8 A n one or mora bacteria! Bralio. in another mb dime t, ike BCA is one or an at bacteria! sixains including but t anon d to Bacillus agri, Bacillus aisa ai: Bacillus aibo!aa A, Bacillus c agu ns:. Bacillus enBaparaslBcus, An-.; Aw smdorhylhtms.. Bacillus i Bdrma , Bacillus kust kl. acillus A/ oA ·. Ba illus laciimorbas. Bacillus laaib, Bacillus iatcrcsporus, Bacillus A?gneo?Aes, Ba illus ¾Aon(AemA, in particular irs in bp '.oar. product known
Figure imgf000029_0002
Biofnngieide or Green RAoa; bvm Nowsyure Bio!ogicals, US)..Bacillus ¾e<0«n. Bacillus megacriu?n Baciilm mcBcnp Bacillus nam,. Bacillus nigrifka . Bacillus papillae (also known as FaenibaoiHns popidiae, product known as Milky Spore b a St. Gabriel Laboratories), B ciil siamensis., Baciilm sphaeric , m pariicolae Serotype BSaOb strain 2362, ('product known as VgetoLoAA bom Vaicnt BiosAeoees, AS}, Asc Av stAA/A roe. on?; · Bacc a, vn=: strain I .Go- a (products known as TaegroA Rhizopro, bXK'SA. ··.··'¾; ikuringknsii.. in pamcntar a ΑΑ;· tharmg mis
Figure imgf000029_0003
·· - oa k so e (serotype ! A 14), in particular sirs in AA!nSAa (Accession No. ATCC 12Ί6, pt xincA known as VcoloBacA fr m Va!ens Biosciences, US) or strain BMP la A (product known as Aquabao fro Becker Microbial Products IB), Bacillus ihorirtgieusis subsp. izawai strain ABTSA8S7 (products knows as XeAl rA" from Bayer Crop Science, DE) or strain OC-91 (Accession No. MCTC i ! 821), or serotype Ϊ--7, (products known as F!orbac WG from Valoni Bosciences, US) or Bacillus tbi ingiensis subsp. kurataki strain HD-s, (products known as Pipe!® ES from Anient Biosciences, US), or strain BMP 123 from Becker Microbial Products, II, , or strain ABTS 35! (Accession No. ATCC SfA!27S); or strain PB 5 i Accession No, C C 72(39), or strain SA ! 1 (Accession No. NRRL BA0A9O;, or strain S 12 (Accession No, ARAL B~ 30/ 1), or strain HA 234b (Accession No. NKRL B- !820rA or Bacillus ihu lngiensA subsp. teneferioms sn-ain MB J 76 (products known as Novodor"' l-C' from BioFa, DB) or Bacillus tlnuingicnsn subsp.
ittor isom o Bacillus tbttringiensis var. $m\ dicgo (product sown as c® from Mycogen
Corporation, US) or Bacihus thsiringiensis subsp, thurlngieflsis (serotype !) MPFLQ 2, or Bacillus ihnringiefisis var. aegypt.il or aclHus th«ringie»s½ var, eolmeri, or BaeH!os thnringiens!s var,
d rmststdieitsls, or BaclHos (hurin ieosA var, dciidroil os, or cC 1.27ft) Bacillus tburingienSiS var.
gaHeriao, or Bacil!us tburingiensls var. japouensis, in particular strain Buibui or Bacillus thurlngicnsss Var. Alls passpucss known as Aataiaic, Poddaa), tsr katnbius tnnBn ionioS WUs ¾6\ r Bianilns
BunirnyJensis strain BD#32 (Accession Ho. NRRk B-21530) from Agmquest, or Bacillus thurtngl«¾sis stratn AQ32 ( Accession Mo. NRKL Bk* 1619) from Agmp,«esk or Ba ilas thanraAensls strain CR--371 (Accession No. ATCC 55273), (CI, 28) Bacillus wnif agdlate, ¾Sftia acidovofarss, m particular strain R Y200 (products; known as BioBoosr), 1. w docao- snbblobcus, in particular ar,un 13-1 · ·.1. Biological Control 2008, -Ιέ, 288··296), Pasteuna pencuans (synors m Baciikrs penetrans), Escndotnanas chloror phis, in particular strain MA 342 {products known as Codornon from Bioagi, S or srnsa> 63-28 (product known as Α'ΓΒκο from EooSoil Systatns, US), Pscndonronas pr radA ·. pn lu-.':, known as Proradix^y Sraptoarvces galhus, in particular strain K61 (Accession Ho, DSrvl 720ft, products known as ycostop', cf Crop Pr moton 2006, 25, 468-475) or strain ΜΡ,ΕΕ 30232. Siroptorayocs grn:eovsridis (products kn n as o ycostop*). Bacillus lmm Bacillus rephaeii§.Ba;$Hu$ mycoides, in particular isolate 3 (product known as Baal (oca Cortes USA) or strain 683 or strain AQ726 (Accession Mo. NR.R.L B21664), Bacillus aoldotcrrestAs, (Bacil!os iastldioscrs, Bacillus aio atertuni (products known as Bsoardy from Bo/Are), or wain YPM3.2S, Bacillus psyclntssaccharolyticus. Bacillus nmroccanus. Bacillus inestatcriaiu C4 Bacillus pankuhemjcns talso known a; Vdrydascilios psntobicndcusy in pariietdar strain Al'CC 14576/ DSM s'dk Bacillus lentns. Bacnlus badnrs, Bacdha: snothk Acinetobacrcr spec, Aeinetobactcr IwoBsi, Bacllios lucitcrcasis, Chromobactaoaam subtsugae, tn particular strain PR A4AT (product known as Grandevo. from Marroftc Bio Sanovailoua), Pas tria osgae (product kn wn as Ec nem™ Biological Na¾ak:idc), Pa«nibaeii!us pohsrsyxa, is particular strain AC-1 (product known as Topsoed from tdoea Biotech Company Ltd.), Sertada errtonophiia (product known as lavadso*), Bacdkrs charinospoms, m particular strain AQ746 -Accession No. N. RL B-2 id 18?,
Pseudonaonas cepacia (ex Burkhoidoria cepacia, roduct known as Deny frorst Stine Microbial Products), Bacillus nernatocids, n particular strain BA6, Bacillus cireuians, BrewibaciHus ErtoroKporas (also known as Bacillus laicrosporus), in particidar strain A Bis: da or train MRS 1111 or strain NRS 1 S or strain MRS 1647 or strain BPM3 or sinbn 04 or strain NCIMB 1419, Cor nebacicri una paurometsbokuty Eaciobacilitss acidophilus (producis known a FnltsarC front iaagrosaBndualnas Agrobioiogleas, S.Ak Paoslbacilkw ηίνά. in parHcuiar strain Ό6 or strain BlaDT- 1 A or strain ΪΗ2Ε or strain 46C3 or strain 277 E Pacnsbacli!us vnaecrans, PasteUria nisuAtwau, as parbctuar straat I'al. Pastearia rantosa, dionek Feaicdliian bbaji, Pseudomonas aeruginosa, irs particular strains ¾d or PML Psoudomonas aurcoBloicns, in particular strain TX-3 (product krtown as SpokLesx Bio f ngi ite from Eco Soils Syssatts, CA)f Pscudotnonas fkiorescens, m partictdar strain 506 t products known as Biight aa or BbghtBa" A506 front NuFarm), Rsoudomoaas putidip Pscudoasonas rcsittovorans (products kno n as Solanacurg), Pscudoaionas syringac, irs particular strain MA -4 producs te wn as Bioa vc fro«;
EcoScisttce, OS), Serratia marccsccns, in particular strain SRM (MTCC870S) or strain R.3S, Streptemyees- candidux, in particular strain V 21007-2, (products known as Bio-bae), Sifeptomyees ivdietoy in patiicuinr strain WYCDI OI (products known as ActinovateSPj or strain WY FC i Og duces known as AeBno- hon Roo> Na ynsi industries), Strcpiornyces sitracet!ons, Sireptomvees veaezinBae, X«nor abdas nemat phtla, A grobactcri m retdsofeacter. Bacillus mojaven-sls, sonpeeudiy strain CFCT- 7666, Pautooa agglomerans in pardeulai strain B325 ( ro cts kno n as Bloornbnse Biological f D Biopestieide}, Streptotnyces eotemWensis, Streptomyeos sp. WYE 20 (RCTC 034 IBP) and WYE 324 ( KCTC 0342BP), Bacillus brevis (also known as rev bseltkis brev¼ product known as Brwisin). in particular ¾ii¾in SSS6-3 or strain >SA:> ·; or strain SSA6-5 or strain 2904, Brwlnia carotovora (aNo known as PectobaotorRns caroi v rumj csrorovora (product, known Biokeoper), Xanthonsonas campestris pv vcsCsrofla (product known as Camp ico), Pasteuria r««ifomus, in particular strain Pr.l. Borkhokieria s e ooon A396 (Accession No. NRRL B-.S0319, product kno n as MBF20S AG A! lo -o srrone Bio Innovations), Bacillus Brrnns C CM I A 382. in particular the aporos CoP U.S. Patent No. 6,406,690, prodtK is known as Biooem, VO'T'IVO), Ba illus cereus fsvnonyms: Bnedius endorhythmotu Ba dlas medusa), in particular s res of Bacillus ceo-n strain CNCM i - ! 362 ( f. US 6,406 A90). strain BPO i (ATCC 5S675, product known as Mepichior from Arysra, US or Meppkrs, Micro-Flo Com an LLC, US), Bacillus amyiod uef cscn strain !N937a or strain FZB42 (DSM 23 ! 179, product known as Rhko ΥηηΙΦ from ABiTEP, DE), or strain BSk ·.·? strain D74?,. (prodncu; known as BacsiarU d ons I: ·<·.; Crop SoiulioiW, NZ, or Double Nickel™ [Ann Certis, US), Bacillus' sabtilis. in particular strain GB03
(Accession No, A3 AC SDN 397, product known as KodiakA from Bayer Crop Science. OF) or strain OST713/AQ 13 (Accession No. NRRL B-3 1 S B products known as Serenade QST 7 i fc Serenade Soil and e enade Max from AgmQuesp US) or strain AQ 153 (ATCC accession No. 53614) or strain AQ743 (Accession No, NRRL. E-2 i 66§) or strain DB i OL (products known m Shelter from Dagutat Bio lab, I A) or strain DB 102, (product known as AAonds from DsgsOO Bio lab, ZA) or strain sdlC 300. (products known as Subtdex from Becker Underwoock CS) or strain QS1 3OO02/AQ30O02 (Accession No. NRR.F B -5042 K ck W(3 20 i 2 '087980} or strain QST30 04 AQ3000 (Accession No. NRRL B-5045S, eF WO 20127087980), Bacillus purnilua, in particular strain C3B34 (Accession No. ATCC 700814 , products known as Yield Shield© iron? Bayer Crop Science, DE) or strain QST2S08 (Accession No. NRRL B» 300S7, products known as Sonata QST 280 ® Bom \uoA:-w- o US), or strain Bo F-33 ( r duct known as integral F-A.3 Bom Becker Underwood, US}, or strain AQ7 I 7 ( Accession No, NRRL B2 I 662, Pasteuria sp., in particular strait) SD-5132 and Fasteursa sp,, in particular strain PTA-9G43, Agro- bacterium nrdiobaencr. in partieu!ar strain K84 (products known ;w Gadtrol-A from AgBioChem) ot strain K I 026 (products known as Noga!k Becker Underwood), Agrobactcriuni vltis, in pinsicular tbe nonpathogenic strain VAR03U , Aiorhi/obnnn cindinodans, prefembiy strain ZB-SKU>, Acospirdlnm wnoo -nw:. o. KO i i linn; brasdense, Asospiriiinm hsdopraeFerencc, Azo-spiri!lurn irakonse, A¾ospiriilinn kpebaraag ? amba >cr chroecoegurn., ptefbrahsy strait; II 35 (CBCT 4435b Λ? < >>»·'¾·.· a ;· vAelargflg preftaably .·.■ .;■■■ A'I'CC 12S3?; BsoiHus aeldooaldamss, BgeU!as acidorertestris, Batumi aCak;phika>. Bacillus a!veg B&ciliws armnoglucaskiic«s. Bacillus mua:anmiig Bacillus anryio!yticirs (saw known as Bae?Bbiaa!hi« aaiylolybeus). Bacillus attoinT oiybena Bacillus snbtills isolate B246, for example in form of the commercially available product Avogreco Boa; RE at UP), Bacillus taqailewsis. in particular sirs i ΚΠ 094, Baad!as sax strain AQI75 (ATCC Accession No. ;b:c 5;. Bacillus p strain AQi 7? tATCC Accession No, 5500b). Bac llus sp, stain A 17S (ATCC Accession No.53522), Gksconacetobacier diaHgropbicas. Herbaspirilura rnbnsuba!bicsnss Herbaspi ilum seropedic&e., Lactobacillus Sp. (products known as Leetopbnt than CactoBAFlb Lysoba^ter ^ n-sgcnc-s in part icular sirens C3 (ok 1 Ne atol. 2004 fane: 3A3b 233··23Αρ Rhodococcus g!oberuius snann AQ7I9 (Accession No. NB.R.B B21bb:b bora Ag:nsQaeslA 5krepn:nrgycas sp, Strain NRRL B-30145 (Boa; AgraouesAb Sbeptomyces 3cidlseah5es; in particular strain RCA ! 017 (product krrosvn as B1.-O0SEP Bora Marrono R! bmovations), Strejnotnyc«s goslbkiensis,, Streptornyees iaveixkdae, Sbeptomyces; prsaanas (cB 'bbaslnons A and B potent insacbeide Bois S ep aoiycca prsnbnus" Appbad nbcrohioiogy iQAt Nov), and Sirepoasyces rkneaas.
In another embodiment, the BCA is one or mora spore forming bacterium, in amaber embodiment tbe spore forming bacerium is selected bom the group c nsistin of: spore- forming baeterhtrn (B) of the genera Bwiikts is selected from the group consisting oB B cithtsfirmus m n CKC Ci >82; Bacillus; cereas -moo CNCM b-1562, BaciHws n> iiqmil; m$ strain lN¾A7a, Bad/ii y iiq fa ie strain P2B42, AosAbo s bri'Hs strain G803; AacbAne nAb/ strain OST713? AacBay /aarb/as strain GB.A. and Baca!w p mih strain QST2S08,
la stilt another amboelnneng the BCA is a bacterial strain selected from the group consisting of In another enb¾xhn<ont, the BCA furthermore products ased on Bacillus firmus ί including bat not limited to strain CNCM 1- 1 582..such sag fo exampkx VOtiVO™, BioAom) Baeiikrs subnlb GB05 nd QST 713. Bacillus subbiA var. amyloiiqae¾e½ns FZB24 and Baeiikss amyloBpusfaGcas FAB 42.
Bacillus thuringlensis subspecies siaswal kaastakg tenebrioois md israelensls. Bacillus sghaerlens, Ba-.allus pomOns sh'sin GB34 and strata
QST2808, Acirratobacier sp., Pasteuna peaatrans... Btreptamyeas vcnesoelae, Mctarinanua ¾rusopbag b52. bictsclsniko ia Irtseisecba, Baacboniyces Hlgciaus strain 2SB Faeci a¾ycas Bsaiosoroseas, Noaa.iraoa rilcyl, Hlrsaiel!a iSiompsoni Beaoveria bassiana, Beaaveria hoii niaftil Bagenidiani gigantauag Myrotiiecium ccrraearia. and Tsukaaarcii pataoractaboSa,
A /brpg aa;. y-i-soo'
in oee csa odimc , the BCA A one or m ra bagi or yggsts. la yet another aitubodlment the fungi or ycgsts inchide bat are not limited to: Ampe!omyces q« ¾«&1i«s ia particular strain AO b (product known es AQ \(f"h Atireo-basidinm palhbaas, in particular b!astospores of strairs DSMI4B40 or blastopores of strain DSM 149 1 or mixtures the eof (product kno n as Blossom ProtecGO, Reayveria bassisna, In particular srai ATCC 'M040 (products kn n as N-^ar- dd '.; or sirain HA (products known as IGyc hM.. BoisniGyrd) or strain ATP02 (DSM 24665} or strain CG716 (product known as BoveMas;}. Candida o!eophila, in particular strain O (products known as N«xy*f) or strain GIS2 (produces known ;ts ASPIRE*, Dceco GlrG), Coniothyriam rmndans, in panicabr strain COId/M/9^8 { S'M- 9660) (products know as Comacs *'}, Ditap ospho« alopccari (prodncts known as Twist Fungus *), GHocladlurri oatenukmrny in pas teomr strain JJ446 (products known as Prestop w, Lecanicifiium lecsnu (rorsnerly known as Vmfci!!ium locanii).: n parucu!ar aorddia of strain KVOi (products knows? as ycotal® Vmafee* from K expert Arysta) or mn QAQMX9 99 or DAOM21(G9p, Met&rhisshsm anisopHay, in parikaodar strain F52 (DSM 3S84, ATCC 90448,. pro ucts known as ΒΪΟ 1020, MET52) Oscar, acrldum isolaso I ] 330I»9A RSEF 7486 ptodncts kn son as > inn n Masc!e®) MetScrnnkoGa fructiooko us particular dec str in PIRRL Y -307527 iptoducs known as SissmoC), Mlorosphaoropsis oohracca (products, known as Micros *"}, Mu.scoclor ad>us, m particular tr in QST 20299 f products kno s as yds! Cop:. Nomuraea Meyy ITS particular strains SA86UH, GUS74 I, S S6151, CO ! 28 and VAO!Ok. Paeciiorrsyocs P!aclnus, in particular spores of F. lifaclmo- strai 2 ((AGAL 89/030550} (products known as BloAcC, cf Crop Protection 20 8, 27, 352-301), Paocdornyeos Rnnosoroseus (also known as Ga a Onnosorosac), m particular strain apopka 0? (ATCC 20874) (products known as PFR-97T 2(>¾ WDGs reFeRa! WO,), PeoiciHiym OdaiL in particular strain ATCC22347¾ {products known as JumpStar , PB-50, Provid*), Piehia anorns!a, in paruetdar strain RL-076, Pseudosvma flooc km, in psrdealar istraio PF-A 2 Ut (products known as Spooadew ^ L.), Pytitiom okgsndnnn. in particular strain DV74 (products known a Folyversum) or atram Ml (ATCC 3H472), Triehodcnria acpordhrrn, in particular strain ICC 0]2 (also known as Trichodorwna harGanum ICC012, products known as Bioten ¾} or strain S T-d (products known as TrldevrnaaP or ECOA-!OPE ®) or strain T34 (products known as T34
Bloconirol) or strain SF04 or strain TV] knowst as Trie o er a Grid TV!) or strain Ti I ialso : nooo as Trichodorrna vlride T25), I richoderma i s: wa ao. in particular T. asiiamun T39 (products known as inchonex <s) or smiln ί 27 (products known as PLAOfi Mi!kLD ®TOi2G, Rootsrucki, TurfShield), or strain TM 35 (products known as ROOT PRO #) or strain TSTI O/FTA-0317 or strain 1295-22 (products known as BkC'fYek), Beauveria brongniartii (products known as Bea pm), (C2,23) Aschersofiia aissT dos, (C2,24) fUssutella t ompsoni {products known as Mycohit)., Laganidiyni gigamcum (products known as !,AGINEX*y Myrothecuun s'crrucati strain AARC-0255 { roducs known as DilcraTM), Pandora dclphacis, ITukannsrcHa paurornetabola, in particular sirain C-924 (proikasis known as Heber er *}, ARF 18 (Arkansas Fungus IS), Trichod«rnis alrovinde in particular strain CGCM C1237 (products known as Esqtdvf;3y WP, Sentinid*, TAneuC) or strain NM! <>.
V0S/0023 " or stra;n NM! No. V0rCOO2389 or strain i No, V08R)O2390 or strain NMI No. VO krBiS patem application US S ? iAAFPPiiOt r saa A tPG ;;wA (IM 10 0 0) or srain Ti ί s!Mi AAA-A ; or strain I.C52 (products knows! as Fc;KU;G A. Agnnun Technologies, {products known as EsquiveA) or strain NMI V08/ 0238 or strain NMI V08¾02389 or strain SKT-IFK M P oA I or strain SKTA/FERM p- 16 Π or strain SKTG/FERfvt P- PA 3: (described in jP369i o4) or strain i 33 (product known s SE TINEF ®'> .. Glomus aggregaruny Glonnw annbeaium, Gkamw iiAratsadlcoy Gionnrs mo&seee, Giouuis desorOcois, Glomus claruur Glomus brasiiianuny Gkuna tr >aos|}crum, Gigaspofs nmrgsrka, luKna oo vniosn!hss, blgo|Xsgon. hneoPts iibteopogcm smy pogorp
RbAopogon fuivigieba, HsoHthus tinctorius, Saferodemm e.epat Sc rcxkrma cfcrinum, Suil!us grarsulanss, Siddus pu ctatapk , Laecaria iaocata, kaccatia bicolor Gab inm iavoviHds, Arihrobotrys daecyGidss, Arthrobouys ollgowpony AAkrobotrys superba Asq¾orgdh? ilavns strain ARRL 21882 ( foduG kn ws as AilaAArard'y irons Syngentas or strain AF36 (product known as AP36A Candida sswaus. in particular strain GRRL Y--2 ! 022 (pro-ducts known as BKX3GRE® or BlOCGATAp
Chaeomium cu-praaar Chaotoiuuan gSobosmw, Cbondrostmsum i - iu¾um, in particular strain
PPC2!3A Cfadospomsm cPadosporioPGs stain PB9 (as described in EP2230R18 Ai), Conid bobw obseufus, Crypiecoccus albidns (product known as Yield Ρ!η¾ΦΛ Cryptowoccus flavesce s, pa p nicular strain MRRL. Y- 50378 and strain R.RL YA0A7A Dacylaria Candida, Emotmnshtborn virulema.
Hgrpo ponun? angudb.dlac, Hirsute-Hit m'm-neso«osis. Hirsute! la rhossiiiensia, Mcristacnmi
asteos ermuTO, Aticrodocbiam dimerum, in particular strain Li 3 (products known as ANT!BOTAl Agrauxina PAstnawosporiuut cionooagnnp Monacrosporkurs psycbrophdcny Monaorosporium drccbsGn, omtcrosporitan gcpbyropagnny Ophios iroa ϊΡίο ίϊίηηί particular strain F)P? i rodncts known as Syivanex), Paeeilomyeos variotit, particular smsn QAG (produc known as Newsa uany F chonia chk rgydosporia (^VerstiHum ehkmydosporiumi), lAeadosyma aphidis, Stagonospora
Stsgaao pora p as«oH, Taiaromyees i'lavas, in particular strain VI j ?b {'products known as PROTUS Φ), Triohod nna vkido (also known as TVtchoderma ga sii >. in particular sirain ICG 080 (products known as RFPAEDfER5* Wrt BlodcrmaG and strain TVs (products known as ' vlrkls T k Agribiutec). Triehoderma harmatura, isolate 3S21 fickodettna koningd, Trtebodsrma Ugnoruay
Trichodorma poly¾x.-rui«. isolate ΜΪ 20603P (ATCC 204'?5), Trichodet rna stfoinaticurn. Idichodcrara yiretvs (also ksrown as Gbocfadnan virens), in particular strain GLA!i (products Puown as SOILGARI)'*) or strain G41 (products; knovm as S W240 WF Biofogicai Fungicide}, UioGadium oudetnassk, in
particular strain BRIG (products known as BOTRY-AFK! ), Vsr ciHitnti aIbo-atn»m in particular strain WCS850, Vonioiliiuo) biamydosporiunK Verticil !isjm dah!ia ssolarc WCS 850 (products knowa as D ich Trig), Zoopbiora rad leans, Cyl Gdroosrpon heteronema , Exophsala jeansdrneb Exopbilia psscipbiia.. Fusailtirn asporgiias, FswaAnra osysporom, ( r exaoi le the non papasgeruc strain 4'? (product:
FGSACk.EAN) or the non pathogenic strain 2S1/2KB (product knosvn as B!OPGAAA FuaaHuni ·:οΙηηί.: For e &npb kt ? >K (as ·Ο Τ>ΪΕΟ,Ι in paRnf applbation Ugyns iOCA¾UA),
Figure imgf000035_0001
roseam, in particular - a:na 321 U, u-cor b;-,ao.Ai:- i docb kaowa ac 8iO- AVARD), ΡΑηηΑο οηο,ΐ geogenow, ½m»-kict<mu$ Rbsporu Phbbiopsb gigsrboa (products kn wn as ROTSOP®), Trichodermt album p docR known as Bbe¾d®), Trsefcodwna aspcrelkaa (prodtreN kw . as IO-TAMw) ami,
TfteiKXii-rms gamsif (products: known as BIO-TAM™} or fa parbeidaf sta 1CC080 (products; known as BbdonnaA U-^ aeba ekrifor is, Maseodor rosoas srram A3-A A aabon o. NRRF 3054Sy
Noocosiri s a vabrobcta, Psadel!liyro verroicuktum (products knows as Ver iculen^ VerSobban beaob acduamoyees: ccrevisae, b particular stra CNCM No. '■■ A* A. >aaAs CNCM No, :■ or- X Arab; CNCM No, PARiPA srain CNCM Mo. U393S (paRsu application US 2011/030)030), and Sporoohm baa ca »;.·;> (prcxhsds known as SpooabocF ;
\n another em odiment* he BCA bdudos aa wiiimgai biocontml agent selected from the roup consisting of Ampdon ces qulsq aUs ( f. AQ I0¾! from Intrac om Bio GmbH & Co, RO„ Oergaayy Aspergiibs flavus (e.g. A- i ..oOCAAlC frosrs Syngema, CH), Aurso&asidnmi puilufan* (e.g.
BOT ECTOR* from kiod rm GmbH, Germany), BaebFas pnnohrs. fs.f. NR.RL A c ssi n No. &-30087 SONAT A* ami BALLAD* Plus from AgraQuest inc., USA a Bacbbs snbibis Cry. isolate NRKl ..-Nr. B- 21661 in RHAPSODY®, SERENADE* MAX a ad SERENADE* ASO from AgraQuest bo USA), BiiciUm xu i!is να my iikisefaciem FZB24 (e.g. TAEGRO* Corn Novoiwrne Bblogbaly inc., USA}, Candida o opbda FS2 (e.g. ASPIRE* f om w;yoa inc., USA), Candida saitosm fog. BIOCURE* s in mixture wish !ysozyme) ami BIOCOAT* from Micro Flo Company, USA (BASF SPA and Arysia), ChiFwan (o.y, ARMOUR -ZEN Irom BoiriZen Lwb NZ), Ckaw.>stachys ros<aa f. oabmdaia, abo n.awal Gli eladar raaemdatans O.g. isolate J 1446: PRESTO!** from Verdera, PiftkndX Coobihyman m it iw (e.g. CONT'A. Ss: born Prophyva, Germany;, Cryphonectrb psrabbea (e.g. Endobb: panwbca f?om CN!CM, France,!, Crvpiocootns albbvw (wy. YIELD PLUS'5'' from Anebor Rio Tecb«oio ios, South ίΑ. Pusarbm oaysporuro (e.g. B!OPOX* ftciti SJ.A P.A., Italy, FPiSACLEAN* from Natural Plant Protoeiioi), France), Motsrladkowia iractieola (e.g. SB.EMEPO from Agrogreeya Israeli bnxlocbbrn dbneram (o.y. ANTIBOT* Rem Agr«abrn>. Franco), Phleblopsis gigardea (eg. OFSOI** from Verdem, Finland), Ps -id x na floccido (<?.«, SPORODEX* froo Piarn Prodaots Co, Ltd... Canada), Pytba i li aiidraoi U'AM (e.g. POLYVER.SI.%1* from Ren50sio SSRO, Bbproparaty, C¾eh Rep,), Reynoaria sacb!inoswb io.g. REGALIA® f om arf iia Bso!nn vstens, US.A)AralarotBycos flav«s VI i 3d (e.g. FROTUS* irom Propbyaa Germa ), 'Frichoderma as eoiHum SKT- 1 g. Ef.O-MOPE* from Raaoal C smi al IncbsSRy Co., Ltd., Japan), R an¾ ;rido, T, atrovinob LC52 (e.g. SENl'iNEL* i'ron-s Agriarn'i Technologies Ltd, NZ), T, arzianuro ΊΆ22 (o.g, PLANTSHIELD* d.er Firma Bio Works ! c. USA), T. hara&num TH 35 Ug . ROOT PRO*1 tram Mycontrol Ltd., Israel), T. imjanum T- io,«,
TRICHODEX* and T !CHO ER A fnm Mycontrol Ltd., r i aad Msk teshiro Lid., bfael). "Γ, feas¾mi«m a d T. oBga or .-a IdtiCAlCdPhk from A iaam Toetestegias ; a! ¾, T, b r;daaua> 1CC012 and T. Crkk ICCOSO gay. EMED!ER* WP tram isagro Ricerea. bab >. T polyrporam and Id haraanusrs (o.g; Bird AS® from BINAB Bio- I novation AB, Sweden), T. sitoniatieaat (e.g. TRICOVAB* Id n C.ERLA.C, Brazil), T. virens GL-2 ! ie. ; SOILGARD* from Ceriis LLC, USA), T. abide o-.g TRIECO* from Eeo^eose Pads, (India) P g .k, !ndfen, ΒΪΟΑ URE* F f m T. Stanos & C- · Lai, Indian), T. vm' dz TV! (e.g. T, vsride TV from Agribkrtec 4.rl. Italy), and U c!admm ou w osli H. U3 (e.g. BOTEYLAKN* from Botry-Zen ti N¾ in one embo im it, the UCA is is o mm profc&oas. a ysa anor emb dimen:, ihg A is one of more proi xo s ssfeciid fom the group consisting of N s ia ioc ias, Tkehkswia, and
Vdri orphu,
4. im i
la ne endaodhneng■;:·:· BCA is ne or more oCasc;;. in vol a dso a bxxb i ids BCA is ona or more viruar-y selected from the group coas;sti«g of Gypsy moth iLynwntria dispar) audear polyhidrosis virus (NPV '}, Tussock moid (LynusmrLidaa) NPV, HebothK NPV, Pine saw fly iNeodiprion) NPV, Cording moth (Cydia poaioneba) granulosis ims fGV), Adoxophyos orana GV (prodacl known as Capes®), Hodco e a armsgora NPV (products ksown a Vivas ax®, Vivas Gold®) orGamAar®), kpodoptera eaigu NPV. Spodopfera Htiorads NFV, Spodopi¾ra idura NPV, Neo iprion abloua NPV (product known as ASiETi'V™). Heodfprion sertifer NPV product' known ,o Ac -check-C^V Agrotis ■■aao-aa: An nip moth) nuclear prdyhedro is virus (NPV), Aaricarssa gemmata!A (Woolly pyroi modi) mNPV ( roducts known as Poiygen), a d Aatographa e ibV-rrnca (Alfalfa Cooper } raNPV (products known s VFN80 from AgAco!a El Soil
i one ombodimont, the BCA is a vims selected from tba group oonigsb ad) Cydla pomoraslia gramdosrs virus, Adoxophyes oraaa granulosis vbns, fkbeoverps armlgcta auciaopoiyhedro virus, Spodop¾ra exsgua nncleopolyhedro irus, Spcdoptera IrAorails nuc!oopdlyhedr virus, Spodogtera Cru iiiicleopolybedi'o virus, Neodipion abietis nucleopoyhadro virus, and Naodiprion semfer aoclaop oybodm virus.
5. £t t ptith®gmk- w.aiai:;da.-
In yer another em odfpent. th« 8CA ¾s one or mos emomopadjogsnb namgiodas. In stiH anodser Oiibodimeni, d½ entomopathogenic aematddes aro solaciad frost? the group conslsiiag of Soiiiern&aia ssp. (-::- Nooap oUsna sppd, Stein&raoma scaperises. Ss¾?na nii;n fdbae (ί;; N Caia aarpocspsae, products kno o-i as Noaaays;*?), Steinemema carpocsgsae (prodnois kssown as Biooonrroi; oi atacik Cg Ploierorbabdida spp., I½i ¾fhabdsils heu thidis, Eiessinermss spp,, Ampblmamds spp.., Momsls ig scent Agameroiis do-cawdata.. M upasina. vv¾issi; Su ulura spp., S¾usrum cadaracbenso. Porygoderffl&dtes spp. AbbreAapi caaoasea, S n¾m gnjaoeasss, fA btfaasn spp,,, Aarameres ,, Aeaarbs spp,, Gongylonemtt spp,, Pwsrrebatns spp. , Hydro ersbs spp., Cameroma spp., Pbysatoptera spp,, Chawoodbebs ovofilameota. Oyrtopoeciiia p§eu<!ovipara.; ParasitylenotHss spp.. MeopamAPykachas rugtbosi, S«Spbti>¾A ien aas eiongatua, SpbaeruhtnopsA spp., Aiianio-nensa spp., Cyaifortyteaebus spp., Bovienema spp., Para aaphelenelnro spp.,. Parasitomabd it k spp,, Phasnmrhabdltis hes?aaphrodita, Romarwmerrais spp., OctQrayommnis spp., Srdkovbaenrb peierseng srutilurtmnis cuhcis,
CuHcimcrmis spp,, LAapidran nnA spp., iastreaisermis spp. fsornenoA spp., Aeonie ooierrrb spp., Lannoa<ern:tis spp., kieson- rtrsis spp,, Xenorhab-daa kanhieseence (en;ona>pa!hogen bacteria symbiotic-ally associated with nematodes); Heerorhabditis baeteriophera f products known as BAAeerc Aemasyog; fj) 5 HsierorhaMitss 'baujsrdi, I-kierorhsbdstis indiea (ptodocTs known as Nematon p
Heierofh&bditis rnaroA-ios, Heterorhabdius ategidA, HeerofhabdbA aealaadAa, Phasnwhabdhis hem;aphredha, Stemeriensa bibioms, Sveinemerna giaseri (products known as Siotopia), Sieneawnia kraussea (products Aawso as barvesure, NeniasysA I,), $iej««m«*«a nobrave (produces known as Bsoveotor) Siebaomosaa seapterisei (products known asNemitoae Sb Sieinem ira scarabaei, Stehteinem sianAayA, BeddAysa {™ Dedadersos) sirkbco!a, iAbpjevhnermis ASpsaadra, and Sidnenwana nhabandse products known as Nemanox® A
A Inmai nts
In one embodiment, tbe BCA A one or mora mocoiants. tn «t soother endsodbweat the irtoetdanrs are selected from the group4 consisting of: Rbizobium J gyminosarum, pardcaiar bv. vjceae stram Z25 (Accession o, CECT 45851 Rhi bi m irop!ci: Rkisohhw kui, Rhizo&iwi tHfhlu, R sobiv eiii L Rkizobhiwfre ii. AixH- bium fsunfiodats, eudo on s, Azospiriihim./iz wbacler,
Strepton;.y ef>, Burk 'di., l.priAaaao'aa/rr E io AAw AiAo. E f Myc^h a esa:aA:a- Arfoisc ia? Abb Myco hiz , and Baifyrhisobhtw.
A okaticab
in one embodiment, the BCA is one or snotx- botanicals. In yet another embodiment, the botanicals are selected from the yecep consisting of: Botanicals (or: plant extracts) selected front t e group consising of Thymus oil, A^adirachtin (Neetn), Fyrethi'&m, Cassia nigricans, Quassia amara, Rotenon, Garde., Ouiikya, Sabadi! . in particular Verabin, fAyanht, in psrAai&r Ryanodine. Viseuro albu (misten, taogwort or common tansy (Tanacekan vuigare), Artemisia absnuhiuo>, hbbea dioie , Symphytum officinale, Tropaonbtin nopus, Quercua mustard flour, Chenopodium anthehrunwicinn, Dryopteris idixmas, bmk of Chinese bittersweet (Cefastrus orbieaiatas}, Eqnisetani arversse, bark of Ceiasos angalatus, Laminann (Browa Algae), kginic acid (Brown Algae), ChitnCbalnosan
Cbenopodium q¾inoa (product kiiowa as HeadsUpo Melaleuca, alfernifolia (produ is known as I anore¾ CiOkl k yeaanie oil ίροχΐο, kn wn m Dmgongf1»«CC ) a id flansmi exiraste o naaiaeoel blend of Cdwnopobloro amhookh do.- {prodneta kn n as aqoiasn).
Ik ¼iiftio¾ if-fiiiiiOiid pp jfc«;opgnwes;m
in o«e etrd odlments the BCA Is <mt or m re prodnei* peedoeed by iemorganissm. la yet another embodnriem, the nrodoets prodneed by mieroorpnisms Ineiirding prmeins or secondary metabolites are selected Iron; bw group consodna. of; i ke ma {prodneed ay BrvAnks -.mp. s o us proPaets known as !½ p->wo ki sw Messenger® , Em l y^ ProAe 5^ ;.
¾k A aeelsassstesslly Active Cc»m >s*sds
la one ernbodnriena the disclosure relates to a composition comprising one or more bacterial strains disclosed herein and one r snore agrochemicalty active eonipounds, Agroebemlesdv -;·. η ·. compounds are substances be are or may be used for treating a ¾eed5 a plant, plant part, or ids environment of has sea or plant mekeJIng but not limited to feugic des, actericides. insecticides, aeadcidwa nematleides, mollnsclcides, sabeners, plant growth regulators, piaal mariesus, aheadee; amides ith a known mechanism of action, addinoimi micro rgani s, and biocomrot agems.
In anodarr embodiment the disclosure relaxes to a first som position oo orsbng one or more bacterial strains and a seesutd co osition c omp ising ova; or more agroeheubcaliy acnve compounds, wherein ihe fet and second composition may inhibit lain pathogens and/or improve plant peribrmanew Use of ?he first and second compositions may provide a amy erne, additive effects or nue. b-eph provide a 'Onoa aab o eiteci.
In one embodnnanp the first and second aomposittons can be applied at die same base to ¾ ss-«d, a plant, plant park or the env ironmen of die plant. In another embodnnenk the first composition cen be applied m fv. seed followed by application oi the second composition so -p.- seed, in yet anoihe* emb diment, die second cornposlpon can be apphed to the seed kblowed by application of dee fhw; composition to the seed.
In another embodiment, the first composition can be applied to the pla or plant part ifblowed by application of the second composition to pie plant or plant pari, in yet another e bodimcnk the second eompmbrion can be applied to the plant or lain parr followed by appllcsboe of p>e km composition to the awe; or pi.aa park
In another embodiment he first eonaposstion can be applied to the seed and the second composition applied to the plant or plant p . in ye another embodiment, the second eomposibon can be applied to the seed and the firsi c- -epesife -n applied to the plant or plant pan ,
Any period of time can elapse between application of da; firsi and second compositions Including bat not limited to 0,25 df a hours, 0.5- 1 hoar, 1 -2 hours. 2-4 hours, 4-6 hours, 6-8 boors. 8- 10 hours, 1 - 12 boinay ay β-≥ hears, | ays, ¾-a s, 4*'f days, i-a **> ka, 3- aeks, weeks, I- bb vvyak , Haaa SKwks a d greater ban 32 wacka.
i, Femicide*
la on*
Figure imgf000039_0001
apTochamic&by active compound is a nsagicide. Ex mple of b.mg;ekles include bat ar« viot Hmaatd to tit* f llowing classes and types of fun icides.
tap teAi¾¾?i'y a iis mt iek a i ytiihexis
la one esa odsmeat, iha agoachomicaHy ^oia: compound is an inhibitor of nucaaic acid syntbesia including but no abied to beaai xyb beaaiaxybsVb bapakasata, doyyaeon, dimethinmoK ebaraaob t r&!asyb h mexasob saetaiaxyh am;daxybbl otaracs. oxadixyl sad oN bnie acid.
β) fahiMmr ef?he mi sis and aelt division
in one <:aysbodimany the agroehemieally active eonipoand is aa inhibitor of obiox t id/or cell do ,aa; ia.aadap- bui so iaanad to benoaryb ear)>aad«aias, chbakeaaaole,
Figure imgf000039_0002
sahabox op bbaendaaole, p-iiuasamra t iabend zole, ihiopharaae, iiis haaas-«'uyh? S and soxaoide.
((;} i.ab;'A/a>av a sy?ii¾pkra?
la oae enmodkneab the aajoehenileally aebYft c m ou d is an inhibitor of respiration including but aoi Uabted to difaaaetoi ina bixafca. boxca!kl earhoxin, aanfunnn, bntoisaik fmopyrarn. furameipyr. famiecyelox, isopyrazam (QR-c n^ aaas), ssopyfaaarn (ySaaaaaponenpp aay-a - d oxyearboxaa pemhiopyrad, thiflwzamide as Ofaasp nation inhibkor; airdxu!brony aKoxystrobhi, cy&¾>i¾mki dh«oxya¾sabm, eyiosttobtn-bL isrnoxadons, fenaroidone, fluoxasifobap kresoxina-- methyh
raatoabDOSfrobia, orysssrrobin. picoxy robin, pyracfeatrobia. arai ra a-. ■■■'■·. tri doxy troma a ax CHI- raa H'a i iaakaaa .
{d) Comptnmds that cm fs &ion us m nn npter
\n am embodiment, he a oohetnieally active compoand fta-t b as as an aocoaplar including but i limited to blnapseryL dic!oran, dreobuton, diaoeap. fttt&rio& and atapry!d!nocap, nitroihabisopropyl
Figure imgf000039_0003
In one embodiment, th& s ommnmlly active compoun is as? inhibitor of ATP production iixdadiag bat not limbed to tenths accaate. ienrin chloride, f ntin hyd xide. and ¾iUbk»£&n¾.
0 iiihi i r ofhe amim.* a d md/ r pwud bfaswtthesis
In one snibodi anb the agrochs-asicaby active compound is aa inhibitor of amino acid and/or proieia bosynthesis including but not limited to an oprtm, blastiakho-S, evprodinib kasaganry-ein, kasngamyein hydrochioride hydrate. mepanipyr½ sad pyiarnoihand- fiii ftthihitW >··</ ··>···:·· r ;?^a:acbar la & embodiment, ih© &$rochefttbei!y a«j.ive earapoaad an hd-dador of ai aaj mmom on moiud g bnt not. limited to knpickaba ibaikxoaib and quiaoxyiaa.
(k) JnkiM n qf ike i!pM ifd mem m s mhe
In -aa onvodia-ain-g the agioehea-aaady acti ve' coaapoand aa inhibt r of lipid sad n>eaabraae ¾yndiesis saa dng but aaa iimited to biphen h ctdoaoHa ay aaiikaphox, etri tazole, fcseyi f ^^tyi- aiaadaam. iodooarb. pro a to .. k a boaa koprokao ao; proayraidoaa, paxxaxsocark prapamocarb h dr hloride* phosphorous add and its -sabs, pymaophox pyraaophoa, tolik>ft¾»rQelhyI and vio osoiin.
φ
Figure imgf000040_0001
abn/>kaoi
in om omh dkniax, the agmehemkall saiko eompoand is an inhibitor of eqaosreroi bioyymhes s bscluding but aoi naked to glPimorph, axacoasaxoisx bbcatanoi, mioa oaasiok, •. a-;.-a-a::ad . dickkaxPaaok, dstbnocoaaaok, diakoaaaok, dkrcaaaxok-yb dodoaiorpix aodeaaxah a-cxn,. apoxao«aaok; caxxona<abe. loaariawb icnbacoasasok, fanhaaaaxad. teaproaiday kapropknok fbayaira-oaaaok, da oaxodob da-aba-a-. ilaol&ibb kaxonaxok, fnraona£ok- x hcxaaoaiaoak, nxaadk knax lb sul te, imihsneo sx le, spooaaxoka a ;tcon3:o;; a. myalobuiaad., oaibiiaa. anarinxd, oxpoconaaok. packxbu maoL pefar xoate, peaoonaxok, pipenbba proch raa, propkonaxok, prorhioaomaxok, pyribmkanx pyritenox, qaiax-araok. smaoixanaxok, ^kox:aabay (abacoasxok, irbn fiae,, itrac nazol , trisdinaekxx riadhxooaj. xldaorspk aafhaxkok; mkxaxa, Plbcoaazola. ankonaxok. viniconaxoio ¾ad voncanaxok.
bp / abaaai-i a/ rk s-ak aaabaxx.-
!a oaa sm! xikaest, dao agrochomiea!iy active aoaxpouad s m inhibitor af cad wad s nibesw bxdadiag but not bra bed by benthiavsHeark dietbofcaoarb, <Hm«†homofph, tiaarapk ipr valio¾fbt aatadipropaabd, p i oxins. po!yox nm, gropamocaf , propamocarb hydroshkai . probaaoeark vaiifesabte, validsrnvcin A, and vspiphaaab
;k frikttmem f iiia/iiakaa
Figure imgf000040_0002
lit one si bodsm nt, the- agroeheabcady aedvc coaspoand s an n ibitor oi mss¾nms biosynthesis aa ia- i;aa ha: aoi banded to aarpropasakb didocyniox kaoxinah phdaaikie, pyroqadoa and a-icyciaxok.
Φ CemjMmmis psihte of inducing k t deense
In one crabodiaa^ab the agrocbemicaliy aotiva com nd is capable* of iadaaing a best dafeasa inaluding bai not limited to sika aaibaaxoiar-S anoihyi: probonaaoio, mi dadiad,
pay C;.a?iaiaa ¥
Figure imgf000040_0003
it fa mutiisifn eli
In oao embodsai ab d-se agpochamicaby acfiva aosnpoumi is aapabla of maltisae actba iaciadiag bat no booed to bordeaux mixture, captalkb capiaa., chl rothalooii, copper iia hihaaate. copper oaida, eoppar oaayabiorida. aopgar paepanPioas :-aah■:···, o er hydroxide, copper sulphat , dichlotiuaidd, dabianosa dodina, dodae baa bass, ; coxa a. flnorobdpat, toipat, yaa aia> ;. ρ·; <..aoa> aect te. imby>oPtdi« . h¾mnfii¾dm;i aibe*bsi: ; nhn«!a dlns maeetaity M neo so msneosA manob, m tifsm, metiram zinc. oxm«~e j>per5 pro amidkia sul hur end sul hur ptepsaations Including calcium
peh oPpbaic. bnoan, toi jfluamd, zimb at; ?;ram.
. tttt K'b'Pt € awkks*
la o e embo iment, a fungicidal ooarposif a cars comprise una or more agfochemicalty active compounds. In one mbodimen sh fungicidal compositi n comprises ( 1 ) at least one acrsve compound 1 se cted fr m . Uikmanedp bon-iU. i-d Hq>o c ' ae --> rPovaaic acid r ,4\S' nny ro~ rphes !--2-y! soude iilaxapyroscukp bbaferp fluopyrsaaa isopyntasap sedaxaoe, pa fbikn and peabuopyrad; and (2) a leas one setiv¾- component II, selected from the group consisting of: a sir bilunn; an axoka a heterocyclic compound; a ad BCA,
In oae enkochateaL the sinabisark is selected from Che group
Figure imgf000041_0001
Figure imgf000041_0002
pyrametostrobira pytao>cy str ps a, -mst o>iy- 43 ,5.6aoc?doto-pyndin~2- Kymcthyt)- afiyl] 3.s¼a«e acid methyl es?sr52 2--(S-c avs »2-melf5 i- !K^ siymetl> l -p e f]«.>- aiedioay-aorybc acid methyl ester and 3-avcthosy--2-{2"| --mehoxy--5ometboxyin>i
phencxymethyi]-pheoy!}~ acrylic acid methyl ester.
in oae emb diment, the aa k is sefecieb frof do; group eonsisbtpa of biehkkat:rsaoie:
¾lscon« le and auincona ole.
n one embodiment, the .heterocyclic c mpound is selected from the group consisting of:
cfeafeaoue ipyenketioaey ethaboaara. flotkaib pyrktorph. tebuiloquin. :y-4 blo-c- -nap b-ipa ,o,\.>.. i ethox -- bcovi)~k ^ ilia}
Figure imgf000041_0003
2o( ! -pqSonetlelk^n^ acid msthyKRH ,2dy -teifabydrooiaphthaiea-l -ybamiue (lib)
Figure imgf000041_0004
Figure imgf000042_0001
'-s;yda m M 1 JyVithis ax -y-S-s bpx Hs sttisd (2s ->d!:m h¾xy«pfv i«?ddy (IJd
Figure imgf000042_0002
Figure imgf000042_0003
Figure imgf000042_0004
!n embodment, the BCA is selected from the group consisting of oearosdapr pyi-beisinc, Uloc!adlwm oudemansi chitosan. Trichoderrtut airoviridcy Ainpelomyces qins uabs, Aspergillus flavuy Aoreobssidnms puilulans, Bacillus subtil ls„ Candida oteophiia, Candida salt ana, Clonosi&chys rosea f. catenu&ic, Comothynum minitam, Cryphoneeiria psraviiios, Cr tooocc s albidus, Fusarium
oxysporum, Mets hnikowus fru ticola, terodechiuro dkneram. Pbkbsops gsgaotea, Ps¾udoryma tlocoo!oaa,, i hkuo oligaftdrum, fte noutria sae!diaerr sy Tblaooapyces fkvus, Trichodenaa asperelken, Trtchoder a atrovitide, Trichodenna harzianurn, a mixture of l>iehodem>a hsrdanum and Tnehodenna viride, a mixture of Trichoderms poiysporum and Trichoderura harzjanurn, Trie-hoder a saromatieusu, Trkhoderaar vircns. Tnchoderrna virkkc ηηά Trkhodenm viride strai TV ! .
2, Bactericides
In another enrhodlment, die agroebemlcaliy active compound is ¾ bactericide. Bxausples or bactericides include but are not babied to bronopok dkhbrophen. abrspyrin, nickel
dierethyddithiocarbamase, kasagaarydn, oethbinoae, Ibmnoarboxyric acid, oxytctracycline. probeoaaok, irepa3 ay ia, ieclobalane ·\. ρο< r sulphate avid other copper preparations. Addbloeai bactericide classes and representative exanip!es are described below,
(«I
Figure imgf000043_0001
fid ChE) miii s
In one embodiment,, ibe agroehesrbeaily active compound is n AehE inhibitor including but not bodied to carbaasaies. aiaayearb, aidicarb, bcodiocarb, benfbrac riy buiacarboxbro buioxyearboxiao canvayk carbufbratp earbosabao, edho.kooarb, bdnobaeafb, fonmianate, i raiihocanh, isoprocarb, aiethiocarb, medioaryb metolearb, oxanryl. pirindcarb, propoxar, thiodioadx tbsofanox. "1-os-a -ax . rriarethacarb, KMC ami xyhyleaxb: or OfgEt)Ophosphais§, e y acephate, aKBmctbi bos, adnphos (-methyl, -ethyl ), cadua&fbs. c iorethoxyfos, eblortenviaphos? dboribrrdnphos, ohforroephos, ehforpyrifbs (- methyl), coumapho-a cyaoopbos, denssbon-S-- nvathyi, dba iaoo. dicb!orvos/DDVP. diorotophos.
baoebexne dina^thylvdphoa, daobdoe EPN, ethiosy eiho rophos, famphur, teaanbphos, fbe rothKae tbapaca. ibs bisaate, beptenophos, koieiiphos. Isopropyl Ob aaithoxyambaduo--pho phoryn salicylate, ; 'oxathsoa iatdathlon, «seaari aao asetbaaudophoS; roethidathJon, faevlaphos. ua>«ocn topho3, aaled:, • -oa'-bo os:.- oxydemeion-aanhyL paradhon bane kyly phenihoate, phorate.. phosaione, phosmet.
phosphaimdon. ph xhn. pbiauphos (-m«thyl), profbaofos,. propotamphos, prothiotbs., pyra ot s, pyndapheathion, cpiiaaipbos, soifoiep, tebupinmfos,. tearepbos, terindbs, tstrachlorvmphos, thlometon, triaxophos, tridorfon, and vaaddothion.
t¾ <J,'iE4- c-baovbV efa ' iii¾iagoi«&ts
In Ofie eaibedias my ibe agroeheaueafby acbve com ou d is a O ABA -gated chloride charred anta on st including but not limited to orgaaoch riaes, e.g. ehiordaoe, endosaiiaa (a); or bpfoles
(phenylpyraxo!es), e.g. eUhproie, fbyro ii, pyrafluprole. and pyriprok.
rid So iwn ^dieaae/ Mai siris ^/ba&sge^ v-v/aa; cktm i
Figure imgf000043_0002
hi one em odiments the agroehenuca!l active compound is a sodium ebsasoe! modulator or sodium channel blocker including but not limited to pyreihroidsy a g. acrinathrin. aiiethrin (d-ds~aaus. <j- p-rnsb blfemhruo bioaliethrin, bioabedirin S-eydeyxanenyl b o-rosaie bria, cydoprotbria, cyflothrin, cytbahr a (betay cybalotla in i foasa, iarubda). cypennetbriii (alpha, beta., tbeta. xeta}( cyplienotlaso -ΐ ? or;y> ··; -;'ηο··.·:\ρ ddranvb- e. dimeikahray CMpembnn HEZ)-( I s- i ssie i; estenvalerspp οί ί¾Γ Γθχ„ fcttpropaihfSfi k-n- .deoar. tlncytbrirsste, fknnefhrka flnvslinaie (ray-), hakenprox, imiproihrin, Kadcth io. etfjfHnhrin. penneda-in.. p enothrm {(IH )· fras- isomer), prsileikrba profkahno.. p-yrebnin (pyrehnnnb Pyrehrine ?e:aaethrin, RU 15525, sPaihioten, tenkahrin, tetramedmin ρ ; Re isomers}!, aaiomerhrin, P:mst1aihnn and ZXi 8901; or DDT or
Figure imgf000044_0001
Figure imgf000044_0002
in one embodiment., the agaaehenneaUy active compound is a nkotinep e ceey lchoiine receptor agonist including bnt not limited to chloronmotkryk, s.g. aeetamiprid. e!oi knidin, dkropdonnt iaddacsoprid, nitenpyram, Piaeioprid, and nicotine.
In one enibodkneni, the hgrochemscdHy active compound is anostanc acetylcholine receptor monda tor inckainyg but not Ite e to spiooyns, eg, s i etomm sod apioove ,
(f) Chkmrfe chi el tse v&Mrs
In one embodiment, the agroehanncaily active compound is a chloride channel acuvaior including bat not Hooted to avenoecrmadybH-emycins, e.g. absmeePn, emaroec-tin beax te, lepimectin. and aalbemecbm
pry a ί>':·' /o-i.-.?.a'a.!.a knks
In one embodiment, the agrochomicaHy active compound is sjavenik hormone mimic including bra ΪΗ ' limited io hydroprene, kinoprenc. meth prcne, tenoxyearb and pynproaytVo.
(h) Mite k mm mw~$fHK-ifk ( ufii-xke) inhibitors
In on embodiment the agroehemie dy active compound is a noo~spneliie inhibitor including but not limite to gating agents, eg methyl bromide and ofhar alky! habdey msihyi bromide, ch ropienn. snlkapb fluoride,
Figure imgf000044_0003
boarx, and a as a emcks,
fii Sete&h>$ iwmtm miR feetimg bfacke
in one embodiment, iln- agrochemicaby active compound is a selective ho iopairoa kaading block r including bat nos banted in p) metro ine and ilonkamid,
Figure imgf000044_0004
w ihiir-r>
In one embodkneng the agroehewicaHy active oemponnd is a able grs>¾lh infelbltor including bnt not limited to ck>f½teainp
Figure imgf000044_0005
and etoxaxo!e,
(k/ Microbial P.-'v- pcaa-; >·>/ mono/ mMgui wa ss'tr*:
in one embodio-ink the agroehemieaily active compound is a nicrobiai disrupter of inset membranes including but not babied to Bacdlus ihiaiogieiods :asb?pecies >sraelensk. Bacillus sphaerkaa Baeilkn tinaingiemac snhya ·. ··:■■ aka.-oo Baciiiu:; U ingienaa; -abpva. -e- kursndxi, 8,κ din- ttektgieosA eneixb idix aad H ; coop pmensa Cky!Ab, Cky!Ae, CryiFa. CryeAb, tnCbySA,
Cry3Ab, CoylBA Cry¾/"35Abl.
(I) Inhibitors >tf ^komi i .4 TP s^^km
in one m dimen, die ngtOebeubcaUy active «ompou«d h m inhibitor of mitochondrial A TP synthase kick-ding but not l m d to dkfendbiroix orgxnobo rnibekiea, 3;oxeydobii; eyhex dn, fe bukdin oxide, picpoiglte and tetrndbbn.
Figure imgf000045_0001
In o smkodimmt, th agrotenfc&Hy active compound is m u couple*' of oxidative phosphorykition including bid so limited to eh rtenapyr, sulikimmkk and DNOC,
bp) \¾:,XbAc -veep /-Voxd'ox χ· eepOsr .'/i(;¾;?W ΡΡ·<·«;ο·>
ϊη one embodiment, the agfockemictdiy active compound is a nieobnic acetylcholine receptor inckding but not limite to beosuhap, eartap hyxSroc Joride thiocyelaoc and ihiosuUap-s dium.
(o) Ihktoiton vfcMtin i y kesk
n one embodiment, the agrochemica!ly active compou d is an inhibitor o ebitin biosynthesis including bur no; limited to type id s ch as benzoyiureas, e.y. bistriHuron, obkvilua invm. dbluhenziuxea ikioyciexoron, iloienxoucoo, hexaduoiuroo, iutenaroit, novsknxes, noviilteoi -oa, peni uron.
tetkibenzuron, and bfiunvtsrors and type 1 su h ax byprokxxnx
bp Aoiibdi? <η-.ηψ?ί-:
In one ennbodhneob the agovohcmieally active com und is a inoibtlng disT ote k«:b::idlig¾ bid not limited to eyroin eine. in one embodiment the agroehernicahy active eompotsnd is an cedys ne receptor agon -i mcluding but e limited to Ji no Imx-uxknes, clxrooMtenoxide, IxboSemxddex mebtcecyienoiide, and tob ft idc-
In one embodiment, he sgrochemicnily active compound is a ocbepamine receptor agonist clodmg Pot not: limited to anbin .
A.; MitoeJumifr e ryms* imm f inhibitors
hi one embodiment, the 8gmehemie¾0y active compound k a mitochondrial complex ill ibeeiron transport inhibitor ine hiding but not limited to hydrameihyitKax aeequlnooyh and itnacoypyrim; a mitochondrial cmnplee I electron transport mhibbor including bat vmt kmked to MET! acaneides, len;¾¾uhx brimyroxunaie, pyrimkiilen, pyf idaben, tebafcnpy r¾t iodenpyrad and rotenone.i Deois iniiwchondi'lal eompixx IV electron In ibitors lixbiKtme Put not limited to aluminu phoapbuic, calcium phosphide, h s iijfiis, :·:>·:; xtae phcaphbde Of eya de; and a mh et nidnid e apPe* e!eeue-n transpor; inhibitor in hadin but not Hrniro<:i a:- cyonopyrales!.
Figure imgf000046_0001
in ne «!i: odsn¾is- b the agrochemc&U actve com oun a vol sw!sum channel blocker cudi g but not limited k> indoxacarb an moi ihuTdaase.
(®) inhibitors ofaeefyt CeA cx?b(?xyia$e
in ofi<s eab-xakmoor. the sgroc-hemio&!ly sebva c ound Is an inhibitor of oet) Ϊ Co.s\ reductase including us n ? limited to ο;;·ΐ·. acid derivatives, spus>dkkbbn, s ir meters, and ien-anbe acal deri atives, a.g. spifoMramai
»} ify S aeeo/ona m * k$hw$
in one sonbodknont, rhe agroc-hsmtcaiy aabve eompomid h a ryanodine receptor modulator in adnpg bnr not fbohod to dianndos, a idoflaroeb cld fansi-asib ioio (Ryn ^ ry C) argrambproja (Cya^ypyf), and ikabendiaabde.
(w) Nmami gM &
in one endbodkritan, the agroeheanieaky activ com ound is a rmiars! cxtracr including but not banted to HEAD$?JP#, which is an extract derived bom the seed huh of hcm>|>x1i u? qoinoa, as disclosed in Lob.6,355,240, thymol, and Pyrethruho hs noes tor crop proasenon purposes are de cribed in U.S.6,743,752 and US 6,582,770; and Isof!avonoids like hpo-ehko-ohoosaechandes,
3, esrsafkidas
fa another embodimen, da- agroehemlealiy aetrvs compound is a nematichte Examples of •)s:¾aticldos include but are not lmited to antibiotic nenmtkkks such as a amectin; carbamate nemadcldes such as benomyl, erb; auras e rboayifbi, and cieotbocard; oxirne carbamate neroabektes such as a!arryoarb, akboarb, a!doxy&arb, oxaroyh organophosphorous ne aticides such as diarnbiaibs, iea:aa;p o>. kssthieia ,, pbosphimbdoa, eadssafos, chlorpyrlkos, diciofontbion, dimethoae, ethoprophos, eosuifbda ia i;xsba:sal.e, heterophos, isaabdob>s. i irotos, methomyi, h ie, phosphocaby lerbaibs, ihiodicarb, thionaein, triasjophos, an eg ah and mecarphon. Giber cornpooads with neanaucidai activity inchide acetopro . bcndokh¾c,y chSoropiefisy da:mmcoy DSCP, DC!F, i ,2-die-ioropropsne, i ,3- diehkaopropene. ftnittrah iodomethane, nei&m, methyl bromids, methyl feoihiocyanate, and aylenok.
Examples of nem&ticides also Include one or more biological nemabeide agents such as
Myrohecmm verrucaria, Surhoiderla cepacia. Bacillus chikmosporys and paecboitryces liiacinu or oesBaticidal agents of plant or animal origin meh as hairpin proteins, amino acid sequeaccs or virus,, vifoid a icles.
■b bateeora
In o e earbcHjlmgm, the agrochem!ctlly active c m ound can bo a safaner. In one embodiment, the agrochemic&J!y acti e ompou d can be a heieroeyOic earhoxyik; aOd derivanva iackdbeg but not limited to dtehb:sropb ny!pyra?oHn-d~ca ox Hc said denvanvex. e. . k(2, - dsckk>ro b5>yiy-5-yoa^ xye sr ooA ackx dOdpyl MOO- OO :· oy-haa -χθθο; a- Wox O . b baa χ·ο4ν· 0ioarboxykae n)ei½pyoOOrhyrk.. aad shruka ompounds f o.;a;: from WO 0(08,74; iky exampO schloro h«a)y!pyo5 j car o yHc oad dero sa b as. e.g, ath l M20- dichloropheaylVS-anedo kb k pyraxdeO<aa0 xybaa ;p I !■. k.aO;.. ido5-.?pOx;y ;-.> Ό'·!' '-p^ Ed i- :: xdy. y · r xylase, ethyl 5 ·· aa-a ey, akO, 00:b ;aak :y. i odOy. : a> r -earboxykte and sbadar compounds known from ΕΡ-Λ 0 33a 131 and ΕΡ--00269 «06; for e.oav-pO ] . s -dipbenyipyraaoOO -carboxylie acid danvaovex, e.g. athyi · ·: 3 kOk o; :-- O /--pOan kdkp;. oa^ . ·-·■· O y;. ];>\·. iOh l f- -'-p aa. ; =·''· ·ρΟ·η aiO- pyraaoOO -carboxyiata, d aaaOr
Figure imgf000047_0001
kaown froa; BP-A 02a 3354; Or eaenapO
a;½s;oOogrboxyba a<. a! derivaives, e,g, OacSPof¾xoO. ieaohloraxeO-eihyb a d · aad a compounds kao«a; ra a EF-A 9174362 and EP~A 0346620; for exasapO 2 soxsaoHneO-carboxyhe acid denvaUvas, eax, edad 5a:2y;0iabk)r bao¾yl)00--cil0i:bx:-' l2'-oxaxoO»3-carboxykne( ethyl S-pbarryl^O-dds drodO'-
Figure imgf000047_0002
and siaidar compounds know from WO 91/03202, or 50-d(plxaayb4yOibydi -- k2-oxaaok
Figure imgf000047_0003
-;Oa Ok propyl \Odnxuaa « οί οθ 2-'-:oo-.: -7..,; ;o .. - kaa ethyl S- (4.-flao?Opbaa>l}0-phaay!- 40 Oihydfo-dO-oxaxoOO-eai boxy late kaoaa irons W 9S9)7890
60 erh>& ?$ gkOoiOioOaaa
la oue endaodimem, the agrachemiaal!y aeOe compound can be a derivative of Oqainolmol ;a udnu; inn s bnbted to derivatives of parKdkos~yOxy)acabe cak a.g. ;x:pX: - - > ; RS- aldof qaiaoaaO- Poxyjaaetate Oekx|anHoaekmexyOk dunedi l enOnO O ay rh a -qaio-aa x- yhoxyjacetapi, (all loxy sburxj C5--eldorr'q aa:dsa yvi)o yj&aeiai:as k('abyOay) r p; s - l p 3· ahloroqoiaolia o4}osyjseetata„ etjry! (5--ahioroq¾naoba-a"-Yl)o yjaaet^ta.: methyl j( ehkxxx:paaoknO- yiOxyiaesintx h S ($--ebk>roqainoHnOa- vl)oxy)acsaxe, 20[propybdanaaraOo]o y Oth> 1 POO cb!or qidssoba-iO !! xy]ac aua, 2-oxopraayi [($- ckloToaiura:diaO--yl)oxy]acetate. arid sbadar o oafKO known from EP-A 0086 "Of; 6:'· A 0994349, ΕΡ-Λ 0191736 or EP-A 0492 ¾ v.eH as bO^WooaquOolbaOy!Oxy] aeetO acid, s hydrai&s and aiday a., . is Ehuiig sodiara, potassianx c lciuay fpagnesiaap a asiaaap iron, aauponSaay qaanaaary smsnoiikim, snPoniam or phosphoaaaa salts a x -a a from WO 02/3404»; for exam le denvatives of kS-etdoroqOnoimOO vl) xy)miilonk- acid, e.g O-x 1 O-chloroqidaoiip-So l)o^y iaudoaaie, dia!ly! (S-abk;aoq5dialin-0.yl)oxyjaxioaate. thvi iueti d |/5'-ebkro<aaaoba-8--yl)oxy4;aaloaate, aaJ aor-d-a vxx;x-; ;d- Κο<ο η 6··· a r Ρ· 05 2 ka.
In a ambodjnasa, the agroehsmioaHy aaiW com und cao be a dOhamxaeetaaPde which are often used as prexinaa¾anee s;ne««x (sod active s&kaxorO. inchalna, bat not lauded to Ohchionaid"
Figure imgf000048_0002
diohloroaeei lOO
Figure imgf000048_0003
netbynx!k;h!oro?:a;aian?da} of PPG laOsstnes, " K'A-2 "
Figure imgf000048_0004
dichjorosceaimido} a; baa- x'insa. t:ADW7" or 2 4660:' ( ai >'oax- t t-bo a- xx¾- sp|m4,5jdeoaaO of itrokeaaia and htonsaato, 0105" ( diet oo.vicOybaxapsaei of rbhxyheunoa
Rib "didoaoa" (dicydonoa) or "BAS 145138" or "Ϊ,ΑΒ145Β8" :^icM røacet !-2:>S,5·■iriiίίeίhy!-!..>- diaaabxyeOO WOjaoaaaO of BASF, OOn!aaoP or "MON 13900" kbx'W.4 , O■;·. -,κ .o : ;-··■·> 3 -Ua; O
2.2-daaahy xas;o!Kbi!o]:, ;a; wad as b;era (R bson?er, aad sWYisybia< addes.
(φ ffydmxyawmatic cemp& ids iWis iSlic-i ipkmic carinmyik d
Figure imgf000048_0005
in oae embo iment the axraxhemieafby active compound n be hydioxyaromata: com o n s and aronssnc-aHphabo oarboxyHo a-, ai derivatives a>cii.aiaag but a--- ibaited as ethyl 24.5- iriaoetoxyboazoate.4-4> droxy- ,S'Xbaajihv;xy e zoic acal lOObyydroxybeaxoic add„ 2,4- d'hydrv-xybanxoia a i .40lyoaad-hydox b£r : e al 2-hydfoxyca!namie ackb 2,4-dchOrodnaaaoe sdd a:;. WO 200 08 63 L WO 20dsPispP4. WO 2bO5AH600ps
faj a 2-0/mOx.'/sba ^Wa - 2· ^ο,χ
lit ne embodiment, the agroehemiesHy active
Figure imgf000048_0006
cars be a OaxHhydfochmoxaikwZona Incl din bat not lankad to
Figure imgf000048_0007
baas-dr F3-(2» bnenylV lO-xbrpsdrochiaox&bndohioae, bO - .auiaoebx hO-dohxav; - I r20;hvdn>ahba>xada-2-osso hydro- chOral
Figure imgf000048_0008
>o. WO) 2005/112630).
of; I ¾aasp/ii?a/¾AyaiSi¾/¾? aaO d> Ov: Wo ·ν
in one embodiment Oe ag}xx:henboaJhy a ex eomaoaad can diphoayimeWosyxeaooe acid derivative including Oa not Haded itjethyl (d! enylmeih xy)a ;i¾ae (OAS-aleg. No.41858·- HW>), ethyl (d?phoi baethoxy ;aoetata or (d henyteasthoxy) .caiio acid (of WO 98/38856).
(g) I t ch n s
la one eaWodnoaiU, das agrochcaneady cti com ound can b¾ a jsoihi<x-hr s¾anone axlubnm but nor babied to nxeih d O-oxo- ihb 245e a 4bio yraaO/3H ;!dene) nnWh<oey]aeeiaO (CAS- ag No. 20512.t-04--6 and similar compounds knoaoa from WO 982133 1 ,
/ft; Nitp fhfrfic αί χ Μ
In oise ambodimeat the agroohamioaiiy aotve coi«po»«d can be compounds tVom the goaap consisting of naphthabe anhydride iaehidiag bat aot banted to (1
Figure imgf000048_0009
acid anhydride), which is kno n s s ed trastmem saieaer fo corn (maise) sgakwt damage by btkwarbamaw herbicides, "teaeionra" { 56~dkhiorv2-ph n> pyrimidin«}. e -.b is ksw n as seed oweewci ssbencr n sown rice agaiast damage by prebkashloo "Uuraeole," which s known as seed uwaaseat s&ieswr for sorgham against damage by ai chhw and meto!aeh!og "CL 3044 ·5!: (CAS-Reg. be k;A -w (4- c&ri>o y~3> ^i df -2H- b K p r∞- ^aoedc aeid) of American Cyanamki, which is known as s-dbaw for corn (maizes against damage by cio w-dasw- ::ybb 1 1" (CAS-Reg. No.96420-72-3} ;2- dictioroaaetiw1woneiiwklA"diosolane} of itrokenray known as saiener for com (masae), "MG-¾3S!! (CAS-Reg. No. irony, ws.w (2-propenyi iwsx3~4-,¾¾is iro[4e5)dec¾r}s-4 ^¾ri dithiosk-} of Nitrokenia, 'diHsoitbewe ^dadieawi-Sw wthyitbioedtyl pinesphowdhhiossey ^drshokdw (Oy'RiiethyhO- phen lphosphorothksriey "nwphenate-" (4"Cideny>henyi--n¾ethyicafbaasate).
< i4) ·: ' -a p.;a;.ds which besides herbicide! activity also eahiba Sabener activity in crops Hke rice, e.g. "i krocpipceac' or " ¥~93" (S- 1 oredhyk i-phenyiethyi-piperidiadxerbodboatef. which is known as safener for rice ayaiast dan sage by nioHnaie, "dahmnon" or "ok 23" Khrnethyk hpherry thyhw-ndyk urea),, which is known as stakeer tor rice agaiast damage by aa-w- -wbaren. "cenwluroa" ~ "hC-AHo" [3- (2- ch!ofophenyhneihyi)-h(l waethyk. l-phsny!«ethyi)u«?8"j ich JF-A 60-087254), which is known ss safener for nee against dsrnage by some herbicides, wnethoxyphenon" or "Hie 049" ·: .'■ .'•'•■.bassb;.
methoay- heneophenoney which is knowa as safeaer for rice against daasage by some herbicides. "CSB" [b'bromo-4- (ebiororaetiryi3tdibayi)ben ene] of eraias iCAS-Reg. No.3409 i --Ob-ay which is kaowa as saiener for rice against damage by ense herbicides, (15) Compounds, which are mainiy used as herbicides, but which exhibit also safeaer aomhty on sonre crops, e.sy (2.4wneh rophenoxy;!aceye ,r. r (2..4-D), i4-ehioropswaoxy}aconc acid, (Ryk)-2-(4-chior-o- iofo!osey ^propionic as si (mecopropy 4~(2,4- dichiorophenoxy)bnryrio acid oka -OH;. (4-ciiioro-o- ioiyIoxy)a.eeda acid (MCRA), 4-(4-<:iiioro-o- toiyloxy hsntyrsc acid, 4y4~chloropiasaoay sbotyric aeid, 3„b-dicbioro-2--e¾etkoayb aaoic acid ('dicansbs and h(cthoxyearbonyi)ebrykk^ Uactidiehbe-eihyiy
Examples of plant groswh regul t rs which may be atemioned are ehlofochohne chloride and odasphon.
Examples of pi&ns netrients which may be mentioned are ensiornar inorganic or organic fcnifeers tor stipplying pkmts with macro- and/or rnieronutrieats,
5« Cora n«ssd« sssed on meehmtem of actios
in one embodimenh the agrocbenkeaily active eotnponnd is a compound with a known mechanism of action. It is to be andersiood that the compounds caa have more than one nesehanism of action.
(ii) M pmti Mtm $
In one ernrx hment the agroehemicslly active compound: ©an be a respiration inhibitor. (Γ) ifthlMtei*
Figure imgf000050_0001
!H O?!* eab:xidbnao. th<? sgrocbcmlealy acdvc mpoimd can be as-? inhibitor of
Figure imgf000050_0002
Π 1 at Qo Site including u n t hmded to strabiksrins,
Figure imgf000050_0003
<;»umt hox i :ro inte. « >x; -5ir.>! b55 dimoxy.wobin.. enssiroburin, fasnoxad ne, ½amidooe, i¾nanbnym>bin:. ienoxysrobbyduf noxystrobiiy S ooxaxb'obby fiyopymnp fyftrxxdde. baklachtodda, kresoxi - eihyi, !wtomim>strobm, orysasirobby pieoxystro bx pyraciostrohm, yramekf$5?<> ln, pyra xysi robin, pyribencarbv rid xysirobhi,
Pi iopyriaarb/cldorodinv jfy .1 bb xbdmiebp bpika:- ;· axoio- - .inxob a .ηη:4;;>Λ ·: :.: ix. acid methyl ester and 2d2-i3-( b<He3dcaO h ! iH^
metlryl-aesisnbde,
Figure imgf000050_0004
acid methyl estcix
Figure imgf000050_0005
acid methyl ester, -x-aadix - a isopropyb-a-oxo d-ortho-toiyh? J--di ydfo-pyra¾aSe~i-.iirbot:hioi- e acid 3 all- <s»tor,, and 3-nsebvx- ·. d: · 12- 2 --msdhox}'--5-(i ei ft ln5in -iY rfj'.y lb berKx mad'iyijqxi'!@nyl}■■- a n be -x iJ methyl eater,
(di) Inhibitors of complex ill M . -.,?··.·.
in om endaodirnent im agrocbemicaily active compound oan be an inhibitor of com plex 111 ai Qi site including but not limited to eysxot bd, amisalbrom, |(3S;dS2R JR)-«def):e b d(3x :eioxy-4- aoxh-xo. -;·■·. i abac 3 oxil n 1 somaa pb anody i-b
Figure imgf000050_0006
j xonan-7-yl] 2-rrieSbylpropaaoatO, jY S$SJRd;43)-eH:^^ 9~ dioxo-1. -d xomsiv7-y{] 2 -methyl propanoaie, f(3S? 6$, 7R ,S 1 )· ·¾κπ2χ3-3·{(3-
Figure imgf000050_0007
,5-dioxomai-7-yl] 2- naathy!poopaoo&ie4(3S^S3?R JR>4xbenxyi-2Hj ~{ ; JdxaaiOihoxohd yhv!e-da ^
carbombjana mo]~6-roe†i i~ 9~dioxo~! ,5-dio.¾?nan«'7-y!] 2-sKeihylprop:aioa!:e. (3S>6S,7R,8 )-'3-|j.(3~
Figure imgf000050_0008
yi "i50t yIpro anoaiiy
(id) Inhibitor of complex I!
in one embobinseai. the agmohewjeaily s nve compound eaa be an addbaor of complex 11 including bus not limited to earhoxamsdey bsnalaxyb benalaxybM, bonodaaiL. benzo ind i ilupy r, bixafeix b scaiid,. carboxim ienlwsnp ieaboxamid, n¾opyraaq totcdanib flisxapyroxad, ftaameip ix isavdardb iso ymxam, kiralaxyb epstmlh me4sia p metaiaxyi-M (meicaoxa ), oxycarboxdrx ofur&oe, xadixyb oxytetracych'ne. penf!ufen, penddapyimh sexJaxane, teck'ftaiam, ba!tkr:amide; ibidimi. eiithioiaare -{ '-
Figure imgf000050_0009
l-bpyraaoie-d carboxandde, N-(2-({, 53- tr Smethyl-buty 1 }-phe¾y i)~ L3 -d iroct¾/l-5-f¼o« 1 H- py razo -4-carbo. n < 3-(dif!uo«>methyiy ! - m«!:byt-N-( 1 ,, 1.^-irimet^Hnd&o^- j^TOxok^^^box^rdde, 3 <(ft-if1 3t?m«t i)-l xmetbyi-N-( 1,1,3-
Figure imgf000050_0010
i yr ;?:oie--4- carboxamiuo.. ··= ;s i ta-oi rxnc;hyi o 1 ^ i'xvdp^ i- d bid- 4; bac:bv yrnia: -a. \ i > ra ·■ 4<. --b a1 ao:.aaodx a- (dif!uo?x>me iiy!}- 1 f$- imst yi~ -(1. i3 rin! ih lbd »-4.-ys)p raao^ 1..\^ u;^k:4: ·. s- ..
• ;.! '·:·;ί!ι·.'; ι· i in:ia- :···: -a 1 ayraa -b- ·. -atanxaada and 2--s nino^« -rne rv ]· dnazoleb•earbaxanihde.
(iVf Other respration inhibitors
in case embodiment, the agroch-smkaUy acti ao-m u d can be a oas imi n inhibitor indduding b wot !iaaited t comple I ncouples,, diflametorirn,
Figure imgf000051_0001
ilnom-4--(4--oifuo^^ nitropheny! derivatives: bmapaaryL dJnobuton, d;noa¾p, n«a.Kiaany brtnaione; eaaa nomePd compounds: eeatia salts, sueh as fenin-crcePite, ientin hlciade .a lcaaa hydroxide.; and amstoeomiin.
(h) Sfent Mmymhesb inhlM rs (S ! ngi d }
la one embo iment, bo aproebemicaiiy active ompound can ha a sterol bios nthesis nbibitoi .
Π; I 4 dcrnethybse inhibitor-,
la one embodiment, the agroehernica!b active compound m be a C14 darneth xe inhibitor includng but aot limited to DMi fungicides, t«&»>les: tee naxo , bitertanol remu£ona¾oie.
cyprocon¾aob. diclobatfszob, difeneconazoie, disiconaxote, dinieona^oie-M, apca¾ccn¾a¾>;e.
el&c a^ob, bn uoonazole, tlaquiaconazo!e.. ikn ao , llamafoi, hexaconaao!e, i ibeneona/ofe. imaa&fii sinlaPa ipconazole. metcoaaaoie, rn.ycfobui.ani!, oxpocomaaab, oxpoeoaaBeSe faraaa-acas, p&eiooutmao ,. penconasob. propiconaxo!c. prothiocoaaao , uiacona?oic, simecona ob. tebeconaxob teiraeonaaeb, aavhavabn. triadbaeaoL trUjeoftazwie, anicoaaxoi , i~[ i?i» S;3 }-'. { 'Chi r hi;riyi)--2-- (2,4-d¾laoropheayn--oxiraayl mei yi-5-ti.)io«y¾nato~ \ H-f 1,2,4 ]tda.aob, .b/rewaaya! K - a i: · crdoropheayi>4y.yeal-aiii1uo irmdtraotes: iraaaa!ii, pd sv- a-a prochioraa, trdUaraaoh ehaboxam, stridise-oie. hysexaaoe, 2abePbro- heny')- Ν·· ;-(4,4- dmseth xyardeen lHS^^ n x afaearaid ; and l-(4-ohloropheay!p2-i 1 H-i ,2,4- tria/cM« i »yi)cycioheptenoie; pyrieaidfees, pyridines and pipsrassmes: fsoariroof, nadmoL pyrifenox. and informs.
id) aitP 14..s¾d atese undbn.,;-;- In ons so^bodimen, the agrochemb&!l active compound can be a delta 1 -reduaisse inhibitor snolucl ing but not iimted to aldimorph, dodemorph, dodenorpbaeetate, ½ipr0pim©rph. tridemorph, fenpfopidin, pipera! , and spirox& ine.
{Hi> Inhibitors of 3-keto ?edacsase
In one embodimeiiL t e agrochem ahy active compound can be aa inhibitor of 3-ket reducase including bat not limited to fenbexamid
(c) Nuctefc acid synthesis W»w
in one embodiment, the agroenenbad activ compound s a nucleic acid symhe is inhibitor ineiduding but not limited to () phenymrmdes or aeyl amino acid fungicides: bena xyl, benalax l-!Vf, kimi&syl ttt&taiaxy). memksyf- (me&ttex&m), &faw > oaiadisyk m<i id; ym»xa¾ole, o tMltnone, oxoHmc acd, buplnrnae, 5-fluorocyto$ine, 5-t1ina>~2-(p-ioiy!ro«fhoJsy)pyritriidin-4--«ms?H\ 5-{1uoro~2- (4^t1uor pii&ny!m«thoxy)pyTtmidin--4-amlne.
In one onibodio-t m, the aoroeh»micaHy aets*o c m ound is inhlbkor of cell division including bui mi Is mi kid to is) tubulin inhibitors, such as benahyud x ks or bsiazisoida^Ie leasin precursor,. dd h !!Oii:¾: benosnyi aarbendaibno debacarb, biPeridasoita i\a\>phanak; Poai covi. !.·-;■· t iopitasiak'- methyJ; tna;-nS pyrimid;nes: S--dtt m-?~(4--meth} pipw 1 f2A
|tna2ido if5-ajpyrio>idi5e: and (ils dlptho%5earb, ehaboxam, peneyeapon. i1uopko!ides soxumi ,
Figure imgf000052_0001
fa.) fHh&i m 'i -i- hi H i
Figure imgf000052_0002
$yHih$.
la oss? oob¾aib-fsenp ih* agroehemically acive <a••nroauri is an inhlbiPr f as'aiao soui r protein synthesis including te& nor Hmiieil to (i) methionine synthesis inhibitors {aalSiHi-pyrimk!inss}: eyprodlniL msp&i'sipyris-ip nd pynrmstha»ib and (it) protein synthesis inhibitors: blaatkadin-S, kasugamycm^ lcaaaporyek;
Figure imgf000052_0003
mildiomy tn* streptomycin, o¾:y;ePacyaiiso po!yoxhte, and vaiidaopy in,
(/} Signet tmm ui'iim inhibitors
in one em odi ent, tha agroehemieaHy aehve compound is a si nal transduction inhibitor including bu not limited to (s) MAP histidias blna.se inhibitors; iluorolmKl iprodiono,
nw ofihkhrisK ptocjamdone, Ylncloiio!in, fatpicienih and fl dfoxonih nd {») G protein inhibitors* pra ox ibo-
(g) lipii sunt f m m synt esis MM m
in orw embodiment., the agfoc ernie&Hy aerjve com und is a lipd or met»brs?58 symbosls inhibitor including but not limited to (!) Phospholipid biosynthesis inhibitors:: edifbiipboP. -pfobanloy rajio b s, !soprothlolaso; id) lipid perovidaPor-: dk ran. nku< <.····.· no tecna onc, kdciofoo moby . bi iion k eh ronah, and eiridkoaals; (its) phospholipid biosynthesis and cdl wad de os hoi
dlsTSith ni h, Humorpk mandipropamid, pynmorph, beoihia.v¾tiearb! fprnvsiicattv vshfenaate and N- ( { -( I 4-cyano-pheiyyl) t:h¾nesulf «yl)-b t-2-yl) ear amk aoiddd-lnorophanyl) ester; (jy> compounds affecting tdl emhrafte penxti-ability and tatty acids: propa oe&rb, prop-uR acarti- ydrochlorid; t'v) fatty acid amide hydrolase mhrhkors: !
Figure imgf000052_0004
I - pip«ridinyil--2-'i5onfcihyi-3ktn
(h) Inhibition with MuMSiie Act m
in «*ne em odiment, the agrochomieaiiy active compound is an Inhibitor with muhi- ka actiwi inch-sdirsg bid not Isnbted to (i) laorg&oie active ub pinous: Bordeaua saixturo. copper aeetako copper hydroxide, copper oxychkxbde, basso copper sulate and lfw, Cm i> t io- and difocarbsm tes: tcrbarn, rnancoxeb, rnaneb, fiwfsm, motbasuiphooiirty melbam, propineb, tbbimy xineb,. srsd xioxm: (id)
rgaaoch! rbio c m ounds (e.g.. phi sbmsd s, abknod-:-::. ddorotbtrfk-yK ani odrie,
eblorothabnil capeafoi. capiara fblpet, dioh!o!lutmki diehiorophen, ftusuffomide. hcx c kaobcnxene, peoiachhrphenok and m salty phdiabds, qaavtcxosftet to!ylfl snid, -(4-chiom-2-ni -s p «?5v1)- »eiyi- 4"R5oth b iinxenes¾r!fofiaidde: (iv) guamdmes and others: gasr-idine. dodine, dodine bbac base, guaxabr-sy aaxaisns-iicoiai , ksdn^oisdine, Ι ί< ή!θ·-!ί1¾οον3ίο, imi«ocvadb!O ris( ib ib k0, dd:hisnon and o diiO nbd} . :bi b-iidb ;;..<>: b >-v > -bi nys rr-ar- i :· o y boanx:.
0) Cil ait synfke inhibitors
in one esfsb diaseny the ¾gr0ch«m diy active comp nd is a ell wail synthesis in ibitor including but ooi limited to (s) inhibitors of g!aeai synthesis: vaHdaniycrsy po!yoxin B; melann s nthesis inhibitors: pyroqciiosy olcyckyolo, oarprop mld, di yciomsi. and fbnoxand; a carboxamide sel cted ikan the roup cond m rPbr?aacbproaribb. and ~(6~ siho¾'y q:5>r;dia-b-yi)cyc!opropanacarlx>^ b s k! smidc.
φ Pi t (Mkme in ues
in orao em odimen , the agroch&mcafly active com ound is a plant defense inducer including but not limited to acibonxoias-S-meihyi, prob na^o!e, isotiadl, tiadsni!, prohexad ne^akiu ; phosphonatos: io¾etx !. fosety!-a.hjmin ?T!: phosphorous acid and its salts.
(k) w« wmte of nation
In on embodiment, the agmehemicaiiy active compound is a compound vvft nknown mode of action including bus not limited o brooopoi, chinomeEhsonat. cyftofemtmid, cymoxaob, dteowei, debae&H di iomoxina, diibnxoquat diienxoqrsat-sTrorb isvdfiits, dipkony!smin, fetip wxmlm, luoriroki. flumetover, i¾¾;.dbnnide, ilutianlL nwtkssuH riy nitmpytfn, nirr^hd-is pro l, oxadbspipoaHn. oxin- copper, p K«im½id, iebtrilo uin, Uiclofta!am, tiaxoxide, 2- irtoxy-6-iodi>-3-pfepyichri>mei)-4-one, - (e do r k ho yinbnobfi^^ scetamide, N:i~
Figure imgf000053_0001
flsjoro< -:r¾] oromcti'!¥bphe«oxyh2J formsmidm®, N'-yx-meth l- -
?riiluoroo^t > -4~{3 ri <^ fomiaraidima '-t'S- diiluoromeihyb2~m formnmidro*, n>et oxy-acetic asid 6-tort-b t)d-S-fluoro-'2>:)-dlmethy]-quinoUn-4-yi ester, 3~S«(4-n-i«Ayi phsn i)-2, - diU!ethyib¾oxa¾adi --3-y!]-pyridine, 3^3-(4-chloro-p enyl>-2 , 3 »d hnethy Msoxagof klin-3 -yl] - ridine tpyflsoxazolep N-(6-wKithOiKy-pyridin-3-yl) cyctopwpanecar xyfk- acid amide. $~chioro-M'4>6- dimeihoxy-pyr imidm-2-yI H-rne& I- 1 H-bwzoimidaK , 2-{;4<.hjor<vphenyl>-N-{'4 3,4Hlimet o y-
Figure imgf000053_0002
N!-(4-i - h Soro-3-trifl aoromethy iphenoxy}- 2,5 -
Figure imgf000054_0001
Figure imgf000054_0002
acid meth KR}~ Ϊ 52«3 ,4-tw»hyiro-RaphthaJen- ! ~yl~aroid«, and N-Metlrylbb^^^
I
Figure imgf000054_0003
cocamidopr pyi bcaraa. coppe '·· 2 rne h bb-trnaiaaiiem for arasdme, and N*~
(5-dinuormedc/b2-cTa y ybbbmed l ibrmamidine. I-
{dsd'd hSor -phea lpy mKhxoi- -ybeb;aa«5¾ O-aiiys- - im, 5-C k>ro- ky4,6 dim ih xy-pyrimidsndk yl}- -medp ~lH~i:snc odda:5ok, 3y2b-dim«thykS-p-ioly1-^ -::··!■·-..·. A- ····'· hyft !)dy/3"d<melh Ms^^ 3-[*k( -mei I-p iiB !}-2 -dhn lhy!dsoxa¾o dia^3-- ylj-pyridne arad dedem i h acetate,
Figure imgf000054_0004
ki ¾sc cmbodb;a:mb the agtoeheabeaHy active com ound is a ayowbi ><y:b..!··: ;· including b t not Hralted to absekk; acid, araidochloiy ancyabdob ^-bsnzylamioop rine, brassmoiak, butra!m, chlorroecjmtt (eb rmequat cfborajey c oline chloride, cyciamlid , da kozide. dlkegy!&c,. dimei ipin, 2,6- dimeihylpuridine. et ephon, fluroefralio, flurprimidoi ikihiacct fbrchiorfeiwixm, gihbvrelHc acid, inabenfidc, mdok-3--a ctic acid, real esc hydrsxidc, mefkddkk, mepiquat (mepiqua* chloride),
«8phib ks5saeeaie acid. -b- eaxyba ^bne, seka; afa¾aL prob¾adkae (prohexadione- alcium), pfohydrojasmon, ibidsa:a <ay $rispenthen«->i, tribirtyf pbospiwrotrithk&r?, 23,S tibodoben¾ok a id, trincxepac- dwi aad im c¾3£ob.
bib l^b fe
ki -air em odiment, die agsxicbemifiaby sative c m c¾«d is a bebbcide kakdkg bm i>'i bamed to (j) acetnmides: acetoc Sor, a!sehior, bsdachlor. dim«†h¾e-h r, dimctbenamsd, bafbfiac«b niefe fa et, rnet kchlor, metaachiar, aapr p rmde, aaproarb e, peli' xsmid, preiHachfor, propaehlop thenylchior, ill} amino acid derivatives: biisnafbs, glyphc-sakc gkbbsmate, s lfbsatc; (lib aryi xypbenoxypropi aws: el dinafbp, oyhakb pbHssyl bbaoxapmp, f!u&»fop, hsioxyfop, melambbp, propaqakaibp, qui;c¾.bjibp, q«i2ak>f p-P-tef«iyI; (iv) Bipybdy!s; diqaat, paraquat; (v) (bb )carbaraae^: asulam. htityiaie.
carb¾iamide, besmedipham, dbricpiperauy epksm (EFTC), esprocaby molkaky orbancarb,
berayiwb haitt, pros ifocarb. pynbubcabydbobeacarb, u fstc; b i)
Figure imgf000054_0005
bufro.xydi . clethodim. cye!oxydiny proibxydi , atth xydkry iepralcxydbyy tralkoxydim; (vii) dkbtroanibacs: beftllura!m, sihaMuraiin, orysbhy pend ethalin, pr dianik«} trtflyrailn (vHi) diphcnyl sihsrs:
aciflu rien, aelooHfen, bift?;Qx, dk!ofop,.
Figure imgf000054_0006
ibmmt½n» isctofen, oxybaorfea; (lx)
hydfoxybcn:coahrH£¾: b moxynll, dkhkbeebb kxynib (x) sa dazoba nijs: isn canielhsi cax, ka jsasvio , jmaxaptc, imasjapyr. ;aia;saquhy iataceihapy;'; cb > pbeat-xy acebe acdi: ciomepst>p, 2,4- dic3borcpheaoxyaceiic acid (2,4· D), 2yi~DB, dich rpmp, MCPA, MCFAdlbosibyi fv!CFB. Mseopmp; fxH) py ih i ehtert smn,
Figure imgf000055_0001
p Hd¾is fsdii) pyri ine: sffimopyrsHd, eiopyrabd. diihsi%nie;¾5s dibdopyy ntnidone, flo xypyr, pidoraro, picoiiaafbi, thtaxopyr; (χϊν) snlfonyl ureas: a idosulfwroo, ss ims»!fbi¾n, bsnsulfuTon, chtorimarosj-etky!, chlorsu!furon, einosultufOti. cyclos |famuro«f ©thoxysolftuwi, ilax syI%'Oiy flyce solfwon, ilupyrsulftimn,
fommsxiIftHOR. h losa!fbi¾!y imasesuifiuion, so^ulfuron, mesosulfuron, metazosuJfttron. metsdfiaxva- methyh mcosulfuron, oxasuH on, jKimisu!furon, prosidfuroa, pyraz suifuron, rimswlfisron,
si fometarofL suifostilfu n, thfcmult oiy triamd&roo, triberfumn. irif!o¾ysuifumn: ii flus f ur r*, iritosuifuron,
Figure imgf000055_0002
2-yI)urea; (xv) riasmes: ametryn, atraarfne, cyana-dns, dime-tlna¾e†ryn.; ¾ihio£m, hexaai onty metamifroa. metribuitis), promeayn, simezine, *rbut >¾zns! terbusrm, triaytflam: (xvi) urea*: eh roiobiroH, daimuron, dm m, fiuowsskiron, iso rotupo , lin ron, iweeha enzifiiMwm ^bMthtuwjn (χνίί) other acetolactare synthase inhibitors: bispyribac-sodtum, cloraosulam-mefhyL dkdosdasry flotamdam, flucarbazoae, flurastsiflani, etosidara. ortho-sallamuron* penoxsolam, propoxycar axone, pyrifcamben*- pi' pyl rsbenio iisy pynfMid, pyrimkiobac-methyi, pyrimisuifan, pyrbbiobae, pyroxasulfcnv, pyr074su!a«y (xviti) ithers: ainicarbazooe, am otriasEoky anifcdby be.0ubutamid< bena&ot y
encarba¾o«cybyatiuresatss eax fesap, bcntazone, beazobicyclon, bieydopyrone, bcom cil
rom bytide, butafeiad, utamifos, eafswscre!e, t¾ri½ira one? cinidoTietfiyf, ehlortbai.. emmethyHru tfloroazone, cumy!uron, eyprosulfamkie, dkamba, dtf«ira>qum, difl«fen¾ p r, drechslgra mo wera*. <<ndothal tho mesate, etobsn anid,
Figure imgf000055_0003
fentrazamide, flumselorac-pentyl, nmniox&zin, fhipoxany flur cbl rid se, fkjriamotse, irsdanofan, isoxaben. isoxaf!ui te, isnaeU, propadk propyzamide. quinclarac, qmmrnt , rnescrtnotie, melhyi arsosdc acid, sapP¾!a«y xadiarayb oxadsazon,
:va' :(a-:^iH-b>ai entoxaz'one, psno adiifL pyra sorbl, pynd1uf½ -ediyi, pynmdfctolsx pyraKoxyfetp pyrazoh¾ate¾ uisoelaramey safiutenaeil, s«k*otHone, stilfmiraitone, ter aetl, iyftsybr ne, tembotnsme,
Figure imgf000055_0004
dihydro-2H^yrimidm-!-yI)-phenoxy acid ethyl ester,€-¾r«ino-S-cbtoro-2- cy !opfopybpyr¾midir¾-4-carboxyIic acid methyl ester.. hi a 3~{2-cych; fop i-6-m«t'h 5- beri y> i1d zs«- -ol, 4~amino-3 ;hioro--6- 4 4 ofo-phenyi)-S-dluOfx>-pyridine--2"Carboxyli,e acid, 4·^::: : : , ·
Figure imgf000055_0005
Figure imgf000055_0006
acid methyl ester.
(it) Insecticides
to. one embwdimeny the agroc-heuucally active compound $ m insecticide inclu ing but nt> limited to (?) organo(thio)phosphates: acephate, azamethlphos, axiaphos-methyk cblorpyrifos, chkayjyrifos-me!hyl, ch rfbnvinph s, diaxinon, dich!orvos, dicroKsphos, dimeth ate, disidfototy eild n, fealirot ijn» t¾fithio;y iodometbane, isoxathiosy ma!adik>«: methaTnido hm, metbidathion, methyl- amdPoix mevjnphos, momjeroto bos, oxydsioeton-aieibyb paosoxoo, p raikfery phemh ste, phossbone. phosrneb phospbarnidor;, pborase, phoxkny piriaopbos-nseUyvb proffenoibs, prolhioiby pyrikipikimsoa, sulprophos, teirachlofvmpks. {«rbui¾s, Mra^etb il tbHn, triazopbos* mck rfon, ·· carbamates:
akaryogrb, aidicarb,. fcandiocarb, beafeaearf eatbaryk ca ofdrasy carboadkay fenoxyearb, fi.;ratbi cafb, nwthiocsfb. rnetbomyk oxa yi. plrkrkearb, pmpoxur, h dicafb, iiiaas aie; (ii) pyrtthroids: a!leih n, bitenthrin, eyf!uthrin,, cyhalothrss). cypbenotbrin, eyperraethrin, slp a-oypennelhrir bets yperme†h ray xeta-syypermeihrin, dekamethrkp eal en valerate, etofenprox. fenpr-pathrin, ffcnvs!entte, im prodma, k bda-eyhaothrio, perrnet ry praileShrin, pyreihna 1 ami !k wmet rm, silafluofeo, tau-i?uvaU«aie, Pclnfhrn, tetrarneiknry traiomeihrm, traiisfluthrir posdluihrin, dimeftuibrin; (iil) i seel owth regulators; a) chkbs synthesis inhibitors: beo;soyhrreas: ckteriluaaurory cyramaxin, ddlibenzyrora flucyxdoxarora fia!Vaoxarors, exatTamims brfcauron, rtovaluroo, tefhsben^uroa, trifla-maron; bapro†e¾a, dioicnokax hexyihiaaox, eirxxaixbe, eiofetaxkie; b) ecdyaone antagonists: hafofenoxkfc5 me†hoays½o¾ida, tebafcnosdde, szadirasbtin; e) juvenoids: pynproxyib nicbo eni;, kknoxycarb, d) li i biosynthesis; inhibitors; spirodiekrfkft, sp ornckfea, spiroteo-amst: (i ) nieotmie receptor sg nsis/sniag niats compooods: ekftfckntdin, dinoie ttfan, i id&cioprkt, ihsameihoxasi;, niteripyraffi, scetatoipmb tbiacioprifiL 1 ~2 hior0 hk¾2ol-5- ln½th ί J J]irtok¾ane;(v} GABA antagonist compounds: endosuifan, chiprok, l pronik vanibpro!e, pyraikspro , pytiprob, 5~amhio-H'2,6-d ichforo- 4-roelh l- hen iH-sulfinaTOoy)-iH-p mwl0-3Hiai¾rtb ic acid arakb; (vj) macmcyekc lactone kaiexaicides; abameetm, eaw«aethy milbeanecthy lepkneetm, sp osad, spiaeior&ay pvk } mixoc drkil electron transport inhibitor (MET)) I aeaneldos: fesazaquia, pyrfdaben, tebaibnpymd, kxlfespyrad, f ufenerim; (viii) MET! !l and !ii compounds: acs-ipms cyk fkaicypr iro , hydr&raetfryinon; (»x)
Uqcouptets: ekiorfenapyr; (x) oxidative phosphorylation inhibitor: ayhesatiry kkdemhiaron. feabmaiks oxide, ptopargke; (xi)mo«ftiug disrupter compounds; eryomazsae; (xii) mixed fas iion osldase mhibitors: pipemrpyi batoxkb; ixkl) sodium channel blockers: iiidoxaaarb, rnesar1umi¾one; {xiv) others: bencbikiax, bdenazate. henebth iy bcnzox.bn.iiie* brorriopropylale, cryolite, pyriiiuqu aaoa. eartap, tloarcaakil pyddajyk meiioa e, su!fiijr, th cyclsm, fiubemiigmbky chlora transkprob, ©y¾?ypyr (HOW86), cyera>pyraf½, i1«pyra¾oI¾s, eyflometof t,. midof nie imieyaiosy bistHfluroj, and pyriiHsqulaaxon, Cyairtraoi!pr ie (Cyaaypyr), Dicofok Ditlovidazin, Fjuensidloaa,, arai Fhtfiprols.
Ck Lp« chiti0lIgo,s«c¾½aHd* mpoisads l d.5 bo
In l nts of ike
Figure imgf000056_0001
family, th* symbiotic iaktraction bew«ert ike plantv sad fikrogav nxing bacteria of the Rkisobiaceae faaiky ' biKobia'") enhance ptemt growth and crop yield. The symbiotic imeraebon k initiated■■■ boa a lan releases flavonotd eoaipouisds that stiaudaie rhiiobial bacte ia in the soil to prodyca ivL€X¾.'! LCOs ere signaling c mpounds dud Induce f s oariy stages of noduisiion in piani ro ts, which load t the formati n of root nodnks comsirdng the oimmenA iog rhimblal bacteria. Application of LCOs 5o seeds of iegtmree and non-legumes sthnufates gemLnafion. seedling mergence, plara growth and yield in crop and horbenbtrfal plant speces, LCOs have also been shown to enhance soot development. Foliar application of L Os has also beet; demonstrated to increase pisoios -a†hesis. sod fulhng aod to e iog in crop and horticultural plant species.
LCOs consist of ao oligomene backbone of j L -linked bi- cciy -D-gioeosandae (^Glc Ac") «?sldnes wgg an Ndhsked fatty acyl chain at the nonredncing end, LCOs differ in the number of Gko Ae resodues in the backbone, n the length and degree of saturaton of live ssfty ac i chain, and in the sabstitotions of reducing and oonredncing sugar residues, ! .Cf) structure is characteristic tor each rhiKobiaJ spees.es. and each strain may produce m iisple LCO's with different structures, LCOs are the primary deteoionants of host specificity in legume sy biosis,
1st one embodiment, hoe bacteral strains disclosed herein east he used with one or snore LCOs. in one embodiment, the disclosure relates to a composition comprising a bacterial strain disclosed herein and one or more LCOs. in another embodiment the compostOn can to fiber comprise one or snore agroehernicahy active compounds. In still ye;' another einbodimeni. the composition can bother comprise one or more BCAs,
in one can- sdnncm. die LCO includes bet is no? limbed to the iLllosvin str ctures:
Figure imgf000057_0001
LCO Structure 2
Figure imgf000058_0001
Figure imgf000058_0002
LCO trict re 4
Figure imgf000058_0003
LCO Structure 5
Figure imgf000059_0001

Figure imgf000060_0001
In another embodiment a composition c¾« comprise one or LCDs includin 'but not limited to the f llowing:
A composition of
Figure imgf000061_0001
Composition LCO H abov
A c omposition of
Figure imgf000061_0002
Figure imgf000061_0003
Composition LCO 2 (above?
61
Figure imgf000062_0001
Figure imgf000063_0001
Figure imgf000063_0002
Oom-postiiors LCO 5 (above)
Figure imgf000063_0003
Ο ο·ρο.οθ<-: i CO 6 < boveo
In one e boddneab the bacterial strains disclosed herein can be us d with ne or more
I oflavones inehidiog hut not limited to gerdsiein, biochaain A 10, dawfeein, giyed in, hei eiodn.
nanog rdrp ebalcooe. coumarin, Amhtol (2--ne†hv!- xid^
dihvdrochoridec a:seorb;u¾ and praiensein or a sab or an esio? thereof.
£. Compositions esmpmrag ΐϊ껣θΗίϊ4¾
b- one embodiment, the iscl sure relates to eomposi ons compridng one or m rs baderiai strains disclosed herieio and quin xoii«eo The oornpositioa c r; fdrther comprise o;e or more agroehemscaily aeaoe compouads rid one or more BCAs.
io c?ic embodiment, the composition comprises a rmxiis e, comprising as acuv compounds ()} at least one compound of formula I
Figure imgf000064_0001
o4)ora : Ri is W or F; R:2 k H or C!-13; R3 is H or CH3: M is CR haiogeru C2U22-a!kyi or CI Coo a!koxy; n indicates e number of subshucn^ R4 on ihe phenyl ring and n s 0, 1 or 2: Fy is pyriirudyi or pyndyL wherein the aforernen boned heieroaroroaiic radicals are ia;¾«bs;haied or carry- h 2 or 3 sden crd o diileram snbyihoeats Ra; Ra is CN, halogen, Ch-C2- ikyb C! -C2-haio¾ikyi or (21432-aikoxy: or an - oxide or an agriculturally accepaible salt of the compound of the fonrada ΐ; and 2) at least one ftwgicidaJty acti e compound It selected from the group consisting of fhraapyroxad, ί: 4 4--^^βξ¾"ί;'"
-methyl forraamldme, N'-(4-4-luoro-3-
Figure imgf000064_0002
-met yl fomsamidme, N!~(2-roathyh5-
Figure imgf000064_0003
oo,, 24
{2'-chkwph^ 24'2-(3-flu rop ersyl)-o- p2-fh.ioroph«nylyoximnysn>vthyi]'-2!3--[i,2,4] 2-[( -fl«oropheny j >3»o»tolyi»
oxlranylmelhyij^ y424 taoro h4¾ l ^ ·· o'iethyisi hu'syl !4 K2y!] azoi«5 l-{2-- 3 lii 3ptenyl}~3
Figure imgf000064_0004
[ 1 ,2,4Jtria2ole. I
Figure imgf000064_0005
-4 H-
1' 1 ,2yl]tnaxota, 1 -H2<-I:dorop & yl)4^^ p.1 pp.
[ 1 ,2„4]trisK0le, booearborhc acid S-{2~[3^2 hloropheoylV2 3-fluorop enyfH>xlr8Rylm«thyi3-2H- I 1,2¾4]ΓΠ- ayohj- l ) oarer ηκΡϊρν! aster, o 3 2.*hi a oe«yi ¾
ih ioeyan&k | H-( t ,2,4 jtnaasj , ihioacelic acid
Figure imgf000064_0006
Figure imgf000064_0007
2H'H'2-ehlo«>phenyl 24;2,4-dli¼>roph ny3.- oxlr¾n½Mth l]~2H^I>25 ]i?)axols- thiol;, 2^2 3y!<hi½oro he^
i;tt2, ]i«83! k-3" thiol, a^oo od orophan !y^
Figure imgf000064_0008
Π; )¾¾& !β, ad3 2-'Chbrop:henyl>-24'2yl-di!la
[ 122,4]maxole(
Figure imgf000064_0009
p jP Qp, thi csrb ic soid
Figure imgf000065_0001
i i .,. ·; · }m¾2 l-3-yj}estef methyl ester, 2~jP-<2«Pdof0 ben i )Pp
Figure imgf000065_0002
{2-chioroph««yl)--3y2ykdieh rophe - 3-.3- e ! n 2-0 ropheny] p2 '4d1won>pheoyl 1 H-[ 1.2, Jtrfa¾ole, 1 ~{2~
Figure imgf000065_0003
< UP ·= ' -;.- v :-V I V· " \ -.}h ::l H I ',··< ·'·.· .'■ }:>'. pHi.': p;■ H · ; ; ;- foiVvl - 1 ! f-j 1 P. U 'k. P-.; .
ihio srbonic a
3-yl}eser met
[J,2,4]tnazoie,
Figure imgf000065_0004
-p-duoro heryP p ilw j ~ί2ΐ- 2-- dPropherrypP
Figure imgf000065_0005
thio arbo c acid Sk
P Py2- oro he y PP2~ite methyl este , 2-
Py2 hPfopnenyp2 (4p¼^ S--PpPP†any k p-- 2 isiorop «nyl^2*^ 2--p P342pP¾PdO opheiy4)--
2--mcihyIpropyii-2-hydroxyPP-d^ dH Py im Pe ~ddone? S-PiProPyP-cPoroP--
Figure imgf000065_0006
N - p2.4--dic Pro h$u ;--2-P)!ish ¾ ■ I -r ePyk <4h p3-(drHitoron 'k;j a compound of fonmtia Ha
Figure imgf000065_0007
hereirs: 8 is a direct bond or n idk iene goaup; A k OH or H; and a HP :\.
\n another erobodknerp compo nd I is selected from the gro p vonspdng of($,8-<!ifH*om- q^u^s¾oknp- k-- d p --trph.!or msd-pd-pyrp| ( ). Pp-diiPoro- uma20Hn-4-yl)-{2-l *-trifto^ p en l] -prop l } -&m \$ i'!-2), PP-PifP ro--
Figure imgf000065_0008
<i-3)„ (5P-Pii1uojo- uh¾82o!m- « i)-{2-i4^^ y-phsnyl --pf'opyk -amine (l~4 5,8÷
H uoro-<juims¾oH^ p eny l]-elh I p«m oe Π-5), (5.8-
Figure imgf000065_0009
phen propyl}~srnine PPy (5,8- din«oro-i}uiK8» iirs-4--yipf2p p en fj-ethy 1 } -amine (i-7), ($β·
Figure imgf000066_0001
-amine (kli * 5 d ditlu r ^ fbms hod- k^^ 2-yhxy)-phenyS j-«thyi } -amine (I-
Figure imgf000066_0002
none (kiddy (SJ»c!IH oro-q inai !in~ -yl)-f
Figure imgf000066_0003
fmvy!kdhyly-amine CM i %
Figure imgf000066_0004
pyridin-.a- yk$xy>-p ig3iiyl}-d yi}-amsne 2»chIom-S-|4--s2d5 U iKJ^ |ui^!m:^H«- .- iam iHihy3]- henoxyBson- sc tnKntd k { da}, i'5,6,S"trif1uori>-- ina iIi^4--y| { 2-i4~(4 i'diiKa- oisdi i pyridss5-2- ylox-- )» h£n t'j-et y} } -amine <M4), (SaaJ-tnduo -qomazo! ^
3-y.dox-- j- hen lp- dsyU-asrdne (MS), (5$j dik fo- n4na;mH«^
Piisnoromeihyhpynd- ba-3- y xy )- j l p^d H -ssmds¾ (kkd), (kS-dbla r -qukrx fda^^
chk.a-^: driilaom odiyi- rkHi -- 2« lox }-3-nH5t>ox -ph&« l|-eih I}-amin« (I- ?). ~- J- -ddl noo
Figure imgf000066_0005
(5,6J rif¼ofo-qidtsaaoIk
Figure imgf000066_0006
- mwit ( id).
in yet anothta- embodiment, eotnpoafsd of tofrmda I, formula Π sod ids BCA as¾ m a
s nergodkaby afiPcbve anv-aat.
f a one mbodiment thi- omp u d \ sod the compound it ¾r re n in a weighs ratio of from 00:1 to \m. in TV eaib da siop the dk ! sBfo rok os aa ihe use of the ba knrd >o ao- diso sod inraio wdh a vonqyosdon comprising an iosectieklal compound of formula IliA. The composition can bather •:oaa ··;-·-? one or nnare sgroehemicady active compounds and one or more BCA-;.
Figure imgf000066_0007
Fornnda kf A
In o embodiment, the bsckrisl strains dke sod herein can be used ssmukanoon-siy, ssquem f with a composition comprising the compound of formnla !IIA. in another ond>odimeop t & bacterial strains can be within the satse composition as the msociicidai compound of formula if in ore; embo ment, the disclosure relates to the use of th - bacterial strains disclosed heoon with a conrpos'Uoa comprising azo pyrmndinyiamine of t nmrjit HI. The cooiposshon can Rather comprise one or more agroehernicalry active compounds and one or more BCAs.
in one eabaodinteng the composition comprises a fungicidal nbxa -c com risn , as active components: 1) a axoIopyrmOdbydamine of the formula lib
Figure imgf000067_0001
n which: R? is Cb-Cc alk i, C;rCR alkenyk C Ci a koy alk CrC,- ycioaikyk phenyl or pheivybCY Cb-atkyi: R3 is Cre mlkyl C: ½~ ikim i, CrC.r-ha a!kyk c ^o .odL y -c b--a!K; ·: where the aliphatic chains in ' and/or R; ma he substitute by o«e to Roar identical or different groups Ra: Ra is halogen, oyamy hydroxy!, mercapRy C:nyy3.-atkyk CrCto-ha!oaskyi, C;-Q-cycio¾!kyL C; Cie tdken h CR C: - ikyayi, Cb-Cy-alkoxy, CrQ-aikykhio. CrC^aikoxy : C^-aikyi or RRARE!: R'\ Rf; ace hydrogen or CrCy-aIkyk where the cyclic groups in R! and/or R:' ma be substituted by one to four gronps R°: R:' is
Figure imgf000067_0002
cy-a!koxy; lb is hydrogen, halogen, cyano, N!RlkR hydroxy!, mercapto, CrC{.-a!kyl, CrCs~ha!o-slkyk C C;rcycfca!kyi, CrC4-aik. sy, CrC alkyiihiO, Q Veycloalkosy, \-o\,- u bi- ho-- carboxyL Rruol C Ciy-alkyRatbony!, C 1 -CK,-aikoxycartK>nyl, Cr- K,-al:keny!oxyoarhoayi, C CRo
a!kynyloxyearbonyl, phenyl, phenoxy, phenylthio, benzvioxy. beoxylfhio, or CkcRaik !--S Obo m is 0, i or 2: A R CR' or and (2) at least one aerive agrochemioaliy acdve compounds of BCA.
in yet another embodiment, the corn position further comprises a compound selected from the group consisting ofc 6-Ry4--dichka¾^^^ -od
Figure imgf000067_0003
h«xy|)~{ 1 ,2,4}triiS2¾>ktj, 1 ,5-a jpyrimidin-?-y- Ismme,
Figure imgf000067_0004
y!amme, ¾-m ihy !- ΐνϊ ! ,2,4]!naiOlof 1 ,S--a:]pyrimidlm7-gdamme, 6- dhy b5--oety! -[ i 52,4]iria2o3o[ I ajpyrimidin-7-y!amine, 5 »t.h}d~-6"oeiy^ 5-eihyR6»(a,S J- triwiort»yl-hexy1 .| lJ2,4]haazolo| l,S--s]pyrimidin-2--yiamme, «-ofcy i-5-prepy(-[ 1 ,2t43trtaJKlo[ 1 ,5- ajpyriroidh -y!a ine, 5-m«h<.>xymeihyi-6-octyI-f
Figure imgf000067_0005
?-ociyi-5- rnf!¾onneihyh l22.4] aaxooi i
Figure imgf000067_0006
( 1 ,2,4 j-txia:oio( I «5-a|pyrii«idin-7- 1amioe.
In one embodiment the compound of the formula JJII and the agrochemcia!l active om ound are in a weight ratio of fwm I 00: 1 to !:} 00. ' . €<3«»pm i&M om rim a fwtyeierie, teals mm anM
In one sroh dinienL the disclosure relates to the use of the bacterial strains disclosed herein whh a m o&idoa composing a polymeric compound., and ionic cotfipoutid or a eoroponmi with imidaaohnm groups. The composition m fmiter comprise one or m re ¾gr chemlcally active compounds and mv o? mere BCAs,
in one embodiment t e com osition comprises at least one polymeric c m und, ionic compound comprising ί mid olams groups (imsdax-oiiuwi compound), whic consists; essentially of at least 8 repeat unts of the general
Figure imgf000068_0001
wherein R; and K' are uxlependently hydrogen and in each ease unsubsiinned or substituted CrCar lkyf C Cjy-a!koxy, C C3o--a!kyl!fno. Ck2;rcycloalkyk Ci-C' s-cycioidkosy, C; 3 cycRcdkylth4o, aryl, aty ioay and arylthlo, R' is b dK^o,€rC?!raikyk CrQwydo&lkyh optionally substituted aryl and a group -c'i l;
Figure imgf000068_0002
R I 52, 3, 4S.5 or 6 and K' is hydrogen or CiRR-alkyl y.awK: the aryl may be substituted by i i. e. d or S radicals selected from Γ C..-. ;.lk.1, CrC. haioaikyL C, -CK--a ikosy,€k c- aioalko y and NR4R", where R' and R'R" are. independently of each other, selected P om hydrogen and Cs-Cjr !k i, each A is independently a divalent aliphatic, aiscyelie. aliphatk-abcyosic, aromatic or araRphatk radical, where the aliphatic moieties in the aforementone aliphatic, aliphatte-alkyche or araRphatk radicals may be internipted by OHO or m e nonadjaeent groups wMeh are selected from --0--, •-S-- arsd ~ (Rt:}--, where R!' is selected from hydrogen, C C(ralk i and a group CHy -R-R) Y-Rc. wherein y is R 2, 3, 4, S or 6 and ! is hydrogen or C C; -akyl: whe? aheycisc or aromatic moieties in dre aiorsaaerasoned alicyclic, aHphHtk-alkyeik, aroatatk or araiiphaiic radicals may be substituted by R 2, 5 or 4 radicals selected from sk-d h w t r..-abow a radical of die formula -() llyCHyOj^-lR1, where *5 is hydrogen or Cr r-alkyi and a: k R 2, 3, 4, 5 or 6: earboxyi and carboxylase, and where the ediphaiic moieties in iho aforementioned aliphatic, aliphati -aikyclk or arahph&tic radicals may bo sabstlttjiyd by R 2, 3 or 4 radicals selected from Cr€;;-a]koXy, a radical of t e formula -O CHyCHR.)],- Ra, where Rft R hydrogen or CrRValkyl and κ is I, 2, 3, 4, 5 or 6, earbosyi and carboxyiate: with the proviso thai the arernavk or nraliphatic radical-? do oot contain 1 ,4 -bound phenvlene rings; and does not contain 1 >4-boumi oheny!enc units and is not 2-hydmxy- propane- 1 ,,3-diyl or 2- ydroxy-2-melhvJ- propane- i3-di l; and where one C! group may be substituted by a carboyyl or carboxylase group; a gyfeup i ich e&ch X fi epcno¾dy Is 0 w §» &«eb B Indc e criy is€ aikv «e; and group of on of the follown f nmd&e:
Figure imgf000069_0001
Figure imgf000069_0002
Figure imgf000069_0003
Figure imgf000069_0004
where # is t attachment pom? to the »mida¾>Uunj ring; and "Vs- s an swalent anion.
I. Compositions comprising safes titated thiopbeoes
In one embodimcrii, the disclosure relates to the use of the bacterial strains disclosed herein with a composition comprising a substituted thk>ph«n©s. The c mpositio can further comprise one or moe isgroc!s micalK' active compounds and ··>>·· or >>.;■■ -^ BCAs.
IEI ooo embodiment, ih composition omprises substituted thiopheics of formula V
Figure imgf000069_0005
wherein: k indicates the nunrber of the oxygon atoms bound to the sulfur atorn of the thiopbene moiety and k Is 0, I or 3: R is C -{OX>Rc or -CH∞ -G-R£; X is O or S; Ri: is hydrogen, CoCd-alkyl C2-CC aikenyL C2 d4-alkynyh -0~RK or C 1·'/" indepeodent!y of one an iher arc hydrogen or CoCCalfcyk IC' is hydrogen or Ci'«C4-alkyi; provided that s!i four R substituents are kiemioah
CtietpDslitosjs cemprbiag su st tut d dithli»e,s
In one embodiment the disclosure relates to the use of the bacterial strains disclosed herein with a composition comprising a substituted diih mes;. The composition c n further comprise one or more agrocherajca!ly active compounds md one or more BCAs.
In one embodiment, the composition comprises cobstlmted dirbdine of !bn ds VI:
Figure imgf000070_0001
here Irs:
k indicates the mimber of die oxygen atoms bound to orre sulfu atons of the dubbne moleiy and k is 0 or I : all four R std-sCmenis being kle ieag and R is C .
C
in ooe embodhnerC the disclosure relates t the use of the bacterial t ins disclosed herein with a composition comprising fiuophyfamm. Bacillus and a SCA. The composition can further comprise one or more agfoehoodeaily active comp unds.
In one embodiment, th§ composition comprises: id ) Fluopyrasn, (2) a spore- forming bacterium of the genera B iiias, snd at least cm? BCA.
ifl one embodiment the spore-dorm ing: bacterium is selected from the group consisting vfMa iiim
Figure imgf000070_0002
strain CNCM C i g y, B&dfius cere . Bacillus omodo Bacillus
iyicBq^d^en , Bacillus sahtiUs strain GBO'k Bacillus mkilis strain CC ' C
In e tb 0 m the BCA is selected Worn the group consisting of: bacterid fungi or yeo , rotos aS; viruses. «niomopaiho^nie nematodes, inoeidants, botanicals, and Harpin f produced by Erwinia amylovora).
In one enpboilimeny the spore-d rndng bacterium of the genera Bacillus and the BCA are not identical. in ne embodiment, disclosure reate to ihe us of he bacterial sin-uns disclosed herein with a composition comprising one or more emirninoearboByi compoun s. The composition can further comprise one or more ¾grochemio¾l!y active compounds r' BCAs-
§n one emi)odra«ftfv the composition comprises; &rs eaam ooarhon ? co pdund of tbrmoja ΠΑ):
Figure imgf000071_0001
wherein R is m thyl, cyelopropyi or 2,2--d!uioroethy;
having the following formrdae
Figure imgf000071_0002
M, C&mposfrkfiis e«R*prfeS»g & str%o cioa« compound ¾tul ;s ftIfd-»lgos«c«;>» id« to one embodiment. tiie disclosure cut to the ass of the bacteral strains disclosed herons with a composition eompsisitig a Hpo-chiioiigosstcchsride. The- composition ecm fimher comprise one or more ags'oohemicaiiy active compounds or BCAs, in one embodiment, the composition comprises a i;0's idtooiig<:S¾eeh;rri;ie of'Sbniinla ( VI)
Figure imgf000071_0003
in which n repressors i , 2 or ; A a sahsdtwem ei½sen iknn RROp, d3($k a Ho-. Rdl RR. -CRtO t 1 R!bOR ·- CO) -! PtbSR ~C(S)S-( RbOtRH-, x'tRRpsgb »«d RsStRHg
8 represents an acvlene; a beteroaryiene con^Rhps i r 2 hetero aroms h sen from okn¾eie oxygen an J sab'hr; a sraphthytene; a beiefonaphdyviene comprising I or 2 cnsro atoms chosen irons nitrogen, oxygen and sidfar; a divaiem mdkad derived from 2 fnsed aromatic nags of 5 or 6 atoms each: a dbaksr adical derived Rom 2 fused atomake or heteroaie aoo dngs of 2 or 6 aionss each, comprising ) or 2 hetere atoms chosen from nitrogen, oxygen and saber; a biphenyiene: or a heterobiphenyRns comprising I or 2 hereto atoms chosen Rons nitrogen, oxygen and sulfur; these groups possibly being substituted with one or two srsbsihuents R1 and Rla chosen, independently of each other, from halogen, C « C(OR>R14, CtObblRtaRiR€F3> OCP3. -Neb: bRR OR S4: $RR NR!SRiS and CRbodkyh
C represents a soNbmeP chosen trom -0-, o?~. R3H2-, -CHR; 7-, ogRp Rig.- and -bRcRh
D reps'esentx a linear or branched, saturated or uns t rated hydrocarbon-based chairs containing irons 2 to 2b carbon atoms;
£ and ii represent, independently of e-'sch other, a ^abstitoeni chose n Rom ih OH. R2R NK2
Rl represents a stsbstituent chosen Rom H. CbR-xdkyb QO)H and C(0)CH3; R2, R3, RR RI4, 15, Rib and R 19 represetn, indepeodemR of each other, a s-tbstRnerst chosen roan !■!, C i -bodkyk bo PCI a ,uk;2. -CfS I diodkyb Xs R d --bodbyh ·χο ΜΡ: -C(S;NH2, RdRR)NH2, 'tORRRb bodkyk R:S. Rd o-eikm and -C(NfH) HCS Xadkyk
R4 represents a snbsbtnent chosen from H, CRRodkyi and R2! ;
R represents a snbsbtaent chosen trosn t Ci Rtcosyi and R22:
R7 represenis a suhsthuen chosen from bb CRRalkyb arabioosyj and R23,
R.b represents a sybsthuent chosen fr m U. CRboilkyb taeosyb nuahyth-ioosyk suitbRtcnsyh acetyifucosyL arabinosyh S03H. S03U, $03Na. SR3R. SG3N(Cl-8aikyl>4 and R24;
RR represents a suosbsaent chosen fro i ti. CRbo kvf mannose. glycerol and R2R
1 b, R j I , R i 7 sn-j R 1 b represent, indessvndonby of each other, a sh iduen; cbnen ο'· so C 1 -R- sikyl and R;
R2b, R 1. R22, k sa R24 and E2S onscreen independeat!y of each other, a snbsbtnent chosen from CXO i R-obkyi, -C(S}CI ^odkxb Ok-s'X; -6-aibyb ·<:ίΟ)ΝΗ2. ~C(S)Ntl2s 4 'iNRydR: - C(0}N tCd b-sikyL -C(S) HC1 -nodi vi and RV HtNHCi -b-atky!; and aRo die nossibla g ssnviricai and/or optical isomers, enarstiorners anddr diasiereoisomes, tautomers, sails, R-oxides, snlfoxides, std ' es, ntetal or tnetsHoid compseaes thereof, which are agrictdarahy acceptssble. N. Comgtt&ttfmis c rising a dlt¾ilis¾- e¾r¾jca lj«siffi!d
In one emhod mni, the disclosure relates to the use of the bacterial strains disclosed herein with a composition c mp is ng ¾
Figure imgf000073_0001
The composition can comprise one or more agroche ieaiiy a ttve comp unds or BCAs.
In one embodiment, the a dithiiisooen acfa'bosamsde baa formula VH:
Figure imgf000073_0002
cal and represent methyl, ethyl, n-propyi or isopropyi and a represents 0 or 1 , or an agrochemaeally acceptable salt thereof,
hi one embodiment, the composition further com rises a BCA selected from the group consisting of ( 1 ) bacteria; (2d fun i, yeasts; and (:>) IsoOavones.
l b ADDITIONAL COMPONEN TS
hi one aspect of the dlsciosotue, a composition disclosed berena may comprise n or more additional com onents}- Preftrabiy, any additional componen is) do nut materially attecr tire anti- ccaiiammant properties of the composition of the present mventio?;.
hi one embodiment, die additional componentis) may be a carrier, an adjavant, a so!ubiliiiog agent, a suspending agerd, a diluent, an oxygen scavenger, an antioxidant a food materia!, an anh- eonmminaot ent, or combinations thereof
In another embodiment, the additional co ponentis) (n y be required lor the application m which the strain or com os tion is to be utilized. For example, if the strain or composition s to be utilized on, or in, an agricultural product the additional ccnn>oagnt(s} amy be an agriculturally acceptable carrier, escip ent or luent. Likewise, if the strain or composition is io be utilized on, or dp a foodstuff the additional component^) may be an edible carrier, exdpient or dbuenb
In one aspect the one or more additional c mponents) is a carrier, excipient, or dibsem.
"Carriers" or ''vehicles" mean materials suitable for compound administration and include any such material known in the art such as, for example, any liquid, gel solvent, liquid diluent, solubilizer, or the like, which is non-toxk and does not interact oath any components ofd.be composition in a delet rious manner.
Hxarnples of nutritionally acceptable carriers include, for example, water, salt sohaioas, alcohol silicone, waxes, peuoleam jelly, vegetable oils, polyethylene glycols, propylene glycol, liposomes, sugars, gelatin, lactose, am lase, magnesium stearate, talc, surfactants, silicic acid, viscous paraffin, perllana oil, fatt ae!d moti myee ides and ag leee-ndes etfoetb?*! fatty acid enters, hydto ymeihyb cellulose,. pc yvhylpsaToPdooo, and die like.
Examples of exeipieots kiolade ut re no limited to: fwjerocrystalhne cellulose and other ce!loloses, lactose, sodi m citrate, calcium carbonate, dibasic calcium phosphate, glycine, starch, milk uucu. and high molecular weight polyethylene glycols.
Ex m les: of diluents seclude but are ml bmked to: water, etbanoi, propylene l col and glycerin, and combinations thereof
The other c mp ent may be used simultaneously (e g. w n they are in admixture together or even when they are delivered by different routes) or secyentially (e.g. they may be delivered by different routes).
The c mp siti n er its adeem may lso contain chela i g agents such as ED'fA, eibac ac d, tartaric acu!, etc. Moreover, the composition or hs diluent may contain aepve agems selected fm laity acids esters., such as mono-ami digdyeeiides, non-iomc suriaci&ms, sucb as pdysorbates, phospholipids, etc. Bmulslfiers way enhance (he stability of the composition, especially after dilution.
XV. FORMS
The bacterial strain aad/or compositions ma !>« used in an suitable lorn? - vheheer ··'· hea alone or when present in a composition. 'The beeleriai s ream: and/or cougsoshtons may be formulated la any suitable way to ensure dost the composition cornps ises an acm e compound: s'i oH er-ess
t he bacterial strains and/or compositions thereof may be in the fonts ol a dry powder thai c n be sprmkled on or mixed in wkh a product. The bacterial strains and/or compositions lis bu: form of a dry owder may include aa additive such as microcrystalline cellulose, guns rragacanth, gelatin, starch, lactose, alginlc acid, Primoge!, or corn starch (which can be nsed as a dislntegmbag agent),
in yet anothe embodiment, bacnsnal strains and/or co pos tions disclosed herein can be a spray, dried iermemate re-suspended m HjO to a percentage selected from the following: .0S-- 1 , 1 - x syf 5 - a 7- 10, 10- 15, 15-20, and greater than 20%, in smother embodiment, one or more than one clarification step(s) can be performed prior m spray- drying,
la one embodiment, the compositions disclosed herein can comprise a suspeobn spores from the bacterial strains disksed herein. In ne embodiment, the suspenesion of spores can be in die range of x l ' - m 1 A I O CFU/mi.
in one e b diment the oornpostions disclosed hcre-e can eoujpnse eoneenirared, era J spores from the bacteria! strains disicsed bereiri. In one embodiment, the concentrated, dries spores can be in the range of ! x 10s to 1 x l:0n CFU/g.
In e emhodlmeot., bacterial strains and/or compositions disclosed herein can be appbod in wet or partially or completely desiccated form or a stony, gel or other form. in at lem some embodiments, bacteria! %w&ins and/or compositions ear- be f eexe-dried or iyphoiiaed. in at leas? some emboditytcnts.. bacterial siraias askbor compositions can be rai sed with a carrier. The carrier ineh.rdes bat is no babied to whey, ntalP>dexirin. sucrose, dextrose, limestone (calcium carbonate), rioe huffs, yeast culture, dried starch, sod soobnm siiieo akneh ate. However,, b Is not necessary to I eetse -dry the strains before using them. The strai s can also be used obth o with u preservatives and in concentrated, erreo^Ksernrated, or diluted form, I» om. embodiment, the strains can be in the form of a pellet or a bioiogicibi pure pellet.
The sirains described herein can be added to one or more carrier. Where used, the «atwr{s> and the strains can be added to a ribbon or paddle mixer and mix d for about S mirurtes. altboagh die timing cars be inoneased or decreased. The components ar blen ed - w. h that a unif m mixture f the euhmw and carriers.) is produced. The final product is preferably a dry, fivwabie powder.
In one embodiment, the bacterial strains sn -r eomposibons may be ibrrmdaied as a liquid, a dry powde , or a granule. The dry powder or granules; may be prepared by means known to those skilled in die arb >;o b as. in iop-opray fluid bed co&rer, a; a bottom spray ofs er. or' by drum -a-annao- -a o .p. high sheer granulation), extrusion, pan coating or n a mlereongfedlents mixer.
In another embodiment, bacterial sirams and/or compositions may be provided as a spray-dried or iree¾wbned powder.
i yet another embodiment the bacteria! saralns nrmd r compositions arc in a liquid ioroHdaboa. Such liquid eonsumpllon may conPbn one or more of the following: a buffer, salt, sorbitol and/or glycerol.
In one embodiment, the bacteria! strains and/o compositions disclosed herein may be fonmdsted with at least one pirysadogicaHs acceptable carrier selected irons at: least one of inaiiodextrin, limestone (ealcmm carbonate), cyciodestnn, wheat o a wheat component, sucrose, smrch, N j Cp, Talc, FVA, sorbitol, ben oate, sorbigte, giyeero!, sucrose, propylene glycol, 1 ,3 -propane diol, glucose, parubens, sodium chloride, citrate,, acetate, phosphate, calcium, metablsulfite, formate and mixt res thereof
In (>ae embodiment, the bacterial strains, and/or eoinposfioris can include a seed, a part of a s ed, a plant, or a plant pan.
V, IMPROVING PLANT PERFORMANCE
All plan s, plant parts, seeds or soil can be treated in accordance with the bacterial strains, ompositi ns ami methods; disclosed herein, ibe compost sons disclosed herein cat! include a plant , a plant pari, a seed, a seed part, or soil The bacterial strains, compositions, and methods disclosed herein can be appl ied to the seed, the plant or plant parts, die fruit, or the soil in which the plants grow.
The plants and their parts may be treated with the described compositions by applying the compositions directly to She plants or plant parts. In another embodiment, the plant and plant parts may be. treated indirectly, tea y t eating the e«vin»am#M habitat in sehleh e plant parts sm or are exposed to. Conventional treaimetit methods: may be ased to tea the environment or habitat mehrding dipping, spo aog. migating, fbggsng, rao nay. brushing, ore iaieefiog.. and, in the case of propagation materia:!, m particular seeds, f thermors by coating with one r mo e eoaas,
in one ernbodimeob the disclosure provides a method of increasing plant performance by applying one or more bacterial strains or co ositions dis losed herein to soil seed, plant, aod/or nbad pnos in ·. ··>·>.··.· embodime t, the discl sure provides a method of isKa¾ebng plant perba-mance by applying one or .m-sv bsaaerial strains or compositions disclosed herein to sod seed, piem and/or plani parts in ass effective amoura :o ineiease plans performance.
In one enibodisneot, die disetosore provides a method of increasing plant heakh b appl ing one or more bacterial strains or compositions disclosed herein no soil, seed, piano and/or plant pasts. In one embodimesrh the disclossrre ovdes a method of increasing plans health by applying one or move bacterial oreao o; composbions a;,;. s.-m ) herein so soil seed, plant, αηόίον plans parts : an elsecbve amount to increase rhsa health.
hi anot r esnbodimenr, the disclosure relates to a composition so improve plant perlbrmanee comprising at leas one bacterial strain selected from the group eosssistnsg of fttebvbi* ;:a eobffopbiea?a.p aciihi iichmifhn , B cui s biins. nd Famib ciii s poiymyxa in an efreebve amccta: to improve plant pesalasaaa.ee.
in one euhoobsoeot, the disclosure provides a method of ines-easing crop or plant yaekb In yet another aspeep the disclosure provides a method of increaarng planr weight or si¾e sedative to a:a untrsateci pistil.
In one em diment she discl sure provides a medmd of increasing cro or plan; yield by applying one r name bactenal strains or compositions disclosed herein to soil. seed, plan or ph t parrs, in another aspect, the disclosure p ovider a method of increasing crop or plant yield emnpns g spplymg at least one bacterial strain or composition described herein to sod or a seed In a ilrsi appiicasbu followed by applying at least one bacteria! strain or composition described herein in a folar application.
io another estybodiroent a bacterial ssmiu or eonip stiion described herein is applied to soil In a bo: application step, applied a:; seed in a second appnesaior>. au-J - applied to die lobar region of a plant In third application The bacterial strain or composition in he I st second and third applications can be the same or different strains or compositions,
in another embodiment, the disclosure provide* s method improving plant perforosatree comprising: (&) spnlylng a first bacterial strain or composition to a seed: (bj applying a seeond bacterial strain os eomposioon to soil surrounding the plant produced bssm the seed; and (e) a l in a barb haetsriaj strain or eomposkbn to an aerial plant par of the plana . The baessrub strain or eomposibon n vis* o o --. w -no and th rd appl ications can be tbe same or different strains or compositions,
in one ennbodirrieiig tbe disclosure provides a method tor improving f>k pettbrn-nmee comprising: (a ) apply ing, a first bav eriai strain or eomposh bn to soil; (00 applying a second bacterial strain or compvsition to a seed: nd (c) applying third bacte ial strain or composition to an aerial plant part of a pfcat produced tknn the seed planted In tbe soil. The haeierbd Sp in or composition In the first second, and third applications can be tbe same or dit etem sussbns or eomposibons.
la another mnbo,0me-us the disobsure provides tor a method tor improving plant porformanee comprising: (a) applying a bacterial strain or composition described herein to an aerial plant pad: of the plant.
In araPher aspeoi. the disclosure provides for improving plant performance comprising applying a bacterial strain or composition described herein to the foliar region of a piano
in another aspect the disclosure provides lor increasing crop y!dd comprising: (a) applying a hbsst bacterial strant or composition to a seed; (b) applying a seeond bavferiai strain or composition, to soil surrounding the plant produced bo m the seed; and (c) applying ¾ third bacterial strain e conmosibon to an aerial plant part of the plant,
la - a aspect, the disclosure provides for method of increasing orop y ield comprising: t a t applying a first bavterral strain or composition to sob ; (b) applying a second bacterial stab or composition So a seed: and prO applymg a t rd bacPenal strain or composition to an aerial plant pan of ss plant produced i f■:·■·.■ the seed planted 1st the soil.
In another aspecp the disclosure provides a method of leoreasing crop yield comprising: applying a bactenai strain or composi'doo described herein to an aerial plant past of dve plant
In another aapset, the disclosure provides a method of increasing crop yield comprising: applying a bacterial strain or composition described herein to the lobar region of a plant.
Its another embodiment, the bacterial strains, comp si ions, and methods described herein increase crop yield by about 10% to about 20%, a out 10% to about 30 .. about 10% to about 40%, about 1 %··. t about 90%, about 20% to about 80%, about 30% to about 70%, about 40% to about 60%, or about 5% or more, about 1 0% or more, about 20% or more, about 30% or more, about 40% r more, about 50% or more, about 60% or more, about ?0% or rnnre, aboot 80% or more, or abotit 00% or more compared to an untreated seed, plant lant part, crop, fruit, or vegetable.
In yet another embod cenL the bacterial s ains, compositions, and method described herein increase crop yield by about 5%, about 10%. about 20%. about 30%. about 40%, about 50%, about 60%, about 70%, about 80%. or aboot 90% compared to an untreated seed, lants plant part, crop, trad, or vegetable. r.-'.-->:\■· 0»ύ %0 &0Ρ 'JCi Pseiei i st PiS, OO pOSOasna, i fid mebtods described hi!iVtiS
Increase plant vpgor by abo t H)% ;.·- about 20%, a o t 10 % to shout 30%, a *s !0% to about 40%, about 10% to abow 90%., about 20% to about 80%, about 30% to about 70%, about 40% to abou 60%, or sbouS 5% r more, about ;P% or more, abo t 20% or more, ah.■■■■ 30% or m re, ,3- 40% or more, about 50% or more, about 60% or more, ab u 70% or more, about S0% or snore, or about 9 % or m e compared to ¾o aatregred seed, plant, plain pan crop, fry it or vegetable.
I yet anoirer embodiment, the bacterial strains, compositions, od method described herein i crease lan vg r by about 5%, about %%, about 20%, about 30%, about 40%, about 30%. %>oet 00%, about 70%. %o>u b0%„ or ab ut 90% compared ro an ontrea;¾d seed, plant pl in ;¾.:··· crop, bant, or vegetable.
n anodeer end>odimeng the baeasrbb strains, c mpositi ns., ami methods described herein merease plant weight aad%r sze by about 10% so about 20%>, abom 10% m about 30%, about 10% io about 40%. about 10% ro about 90%, %··-ο; 20% to about 80%, about 30%. to ab u 70%, abour 40% to about 00%, or abota 5% or more, about 10% or mcrre, aboou 20% or more, about 30% or more, about 40% or more, about 50% or more, abool 60% or m re, about 70% or more, about rP0% or more, or about 90% or :·.:-···/· compared to at) entreated seed, plant, plant part, crop, fruit, or vegeabje.
Irs yd another embodiment, dse %cas up strains, compositions, assd fnothod described heroin increase plant weight anddsr sloe by about S%, about 10%,, about 20%, about 30%, about 40%, ab ut 50%, about 00%, about 70%, about 80%, or about 00% compared to an untreated seed, plan, plant past, crop, fruit, or vegetable.
A. Modssialios of l*lMis ls« *>5ss
Phosphorus is one o; 17 a,ancra.< essemittl tor plant growth, bp, functions cannot be srlbrmed by aay other nutrient, and m adequate supply of P is required for o timum growth and reproduction. Phosphorus (P) r: vtial io plant growh a d is ibuud in every heme plan! ceil it «s volve-d in eovr 0 key plant functions, ncludin ener y txansf r, photosyot es . tmnsfonr rtion of sugars and starches, nutrient movement within the plant, and transfer of genetic characteristics frost one generation to the next.
Phosphorus is classfed as a major otPnent meanin t its ft¾qus«tiy deficient for cmp ptxxluetfon am! is rep;.;li-ed by plants rebrbvoly larg« amounts, the total 0 eommnhauion in agricultural plants generally varies f% 0.1 to 0.5 percent.
Phosphorus plays a vital rede in virtually every plant process that involves energy transfer. Bigh- «n«rgy ph s hate, held as a p rt of the ehemleal structures of ad n sine diphosphate (ADP) and ATP, is the source of energy that drives the multitude of chemical reactions within the plant. Who?; AD?5 d AIT transfer the high-energy phosphate to other moieetdes (termed phosphorylation),, the stage Is set tor many essential processes to occur. !¾:ssphorous k also a component of phvtaa a major storage toon of P in seeds, About 50 percent of the t ps! in legume seeds an 60 to 70 pes ent u; cereal grams is stored as phvtm or closely related eomponnds. An inade uate amount of P can redaee seed s¾ seed numbe , and viability.
1. Ability Solabffim Phosphate
In n em odiment, the disclosure mkies to bacterial strains, compositions thereof, and methods thai can solimiilze phosphate. h\ a ther embodiment, the disclosure relates to bacteria* strains and compositions thereof that can soluhllise phosphate in «.oil. i yes smother em d ment,, the disclosure relates to bacterial strains and com ositions thereo dta; can soiubilix phosphate and reduce h need for phosphate !ertibaer. in yet another embodiment the disclosure relates to bacterial strains and compositions thereof that cars increase the amount diphos hate availa e to a seed, plant, or plant part.
In another mb diment, the bacterial strains an compositions dis l ed herein can increase the total P concentration in plants. In one en^o ime t th total p concentration in plants is increased by percentage selected from the group consisting of from ) % to 3%, mam 3% to 5¾, from $¾ to 7%, from 7% vo 9%, from 9% to ! I %, from 1 1 % 13%, from 13% to 1 so from 1 5% io ! 7%, from ! 7% to 19%, from 19% to 25%, and greater than 25% as compared io an untreated control.
in sub another embodiment the acterid stoma; and compositions- disclosed herem can Increase the total Ψ concentration in phytin. in another embodiment, the total F concentration in phytm Is Increased by a percentage selected torn the group consisting of mam 1% tj a%, fi a 3% to 5%, from 5% to 7%, fern 7% to 9%, fr m 9% io 1 1%, from i t% to 13%: from 13% to 1 5%, front 15% to ! ?%, from 17% to } 9%, from 19% to 2%o. and g; ··;..·. ;er than 25% as compared to n untreated control.
In yet another embodiment, the disclosure relates so methods to increase phosphate. In ·■■·. ;
emb diment, a method for increasing phosphate comprises applying at least one bacterial strain disclosed herein to a seed, a plant, or plant part, Irs another embodiment, a method for increasing phosphate comprises applying a composition comprising least one bacterial strain disclosed hernia to a seed, a plant or plant part, hi still am her embodiment, the method comprises applying the bacterial strain or composition to a seed, a plant or plant part a; soli- In another embodiment the composition further compmes one or more agrochemic&lly active compounds.
hi one embodiment, the bacterial strains for modulating phosphorous are selected from the group i ifisistln or: B i'ius a&yloi;qNify ie arid Backi //c/aeir/ormfr. in one embodiment, the naaterlal strains tor morhtkrtmg phosphorous are selected from the group consisting ot: Baailm saay ten sma.: a ess BS27 BiicUi yloik dfa le BS2084, Badilw r>n7oHquiiacie ! 5AP : Bact//;s awyhli. ifaciem 5.\P f Bacillus cunyloHquifttciens tSSAO i . Bacillus ot hHq hci s ABP2 /8, Bacillus
amyfaHqtw'&B s 1013, Bacillvs y!oik^ija le 91 St and combinations thereof in yet another oanwonneco the bacterial srrams i¾r modulating: phos horous are selectee rAsm the group eonsisgbig oi:
Figure imgf000080_0001
BAS42, aciH s k-hemfhmns BL2 1 , and combinations thereof
. Modulation « f the ?¾>s haie Reguton
Genes bel n ing io ih« pho regalon. including She aikaihse phosphatase (ΛΡ) gone (phoA) and the pbosphate-specifsc transport (pst) genes, are regulated by a {¾o--proteia system consisting of n phosphate se sor protein iPhoR) and a transcriptional activator protein (phoPy Alkaline phosphatase is invo d in the enaymaiic breskdossn of phosphorou
When phosphate conditions are low, the sensor kinase PhoR seH-phi ¾phory!a forming PhoR- P. PhoR-P transfers the phosphate to the transcription iacior pnoP, forming phosphoryiatod phop.
Phosphoryla ed phoF activates expression of about thirty different genes, mc hiding a!ka! e phosphatase, by binding io the pho boxes located upstream of the phosphaie-aegakrted genes.
In one embo iment, the disclosure relates Pa bacteria! spasms nd compositions thereof thai contain the phoP gene, in s-s ahes eamodlment, the disclosure relates to bacterial strains and
compositions thereof that contain the phoP ger-e and aid its the breakdown of phosphate. In yet another mbodiment,, the disclosure relates to bacterial strains end compositions thereof that contain the phof gene and aid la the breakdown of phosphate, thereby reducing the need tor phosphate iertibaer. la still another embodimeap the disclosure relate* to bacteria! strains and coaspoaitions tbereof that contain die phoP gene and ep. n veb breakdown phosphate It) the sod,
la yet an t er embodiment the disclosure relates to methods to aid In the breakdown of phosphate hi one emb dimen , a method to atd in the breakdown of phosphate comprises applying at least one bacteria] strain comprising the phoP gene to a seed, a plant or plant pari in another mbodi ent a method to aid Itt the breakdown of phosphate comprises apphAng a composition comprising at least one bacterial strain comprising the phoP gene to a seed, a plant or plant part, it; still another embodiment, the method comprises applying tin: bacteria! strain or composition to a seed, a plant or plant part in soil, in another embodiment the eomposltloa bather comprises one or more a onomical iy active cotnpearKls,
In still another embodiment the disclosure relates in methods to increase production of alkaline phospbatttse. !n one eehsodn-fiem, a atethod to increase production of aPmbne phos hat se comprises appK Age at least one bacterial strait; mprising the phoP gene to a seed, a plant or plant part, in another embodiment, a atethod to increase production of alkaline phosphatase comprises applying a composition comprising at least one bacterial stram comprising - he phoP gene to a seed, a plant or plant patt. In still another embodiment, the te mod comprises applying lite bacteria! strain or composition to a seed, a plant or plant part in soil, in another embodiment, the composition further comprises one or more agrochemleally active compounds.
Is* one eatbo nrient, the bacterial strains containing the phoP gene arc ft . ; .· oo AAmnmaaeaS . in ot;e embodiment, the bacterial strains oonraln g the phoP gene are selected Irons the group c nsisti g of: B i!iu* itmyktHq iack B¾ r, iteei&s xmyhiiqui/ ck mM4, it«cid¾o
Figure imgf000081_0001
I SAP4, BaeiHus amyhHqu(/( ms 3ΑΡΊ Badttvs myhH <ifh e LSSAOL BaeiM s i w Hgtiifycmis ABP2?I, ϋ- !■<;!■. oU ulfyai s 22CP1, B ciUus a y iiquifc ns BS18, and eombhmtlorrs thereof, B. ottttlatioti of fisni Growth Hormone*
Plant hormones ore sig d moleea!cs roduced widm the pkmt, and occur in v.oom-. ;·, low concentrations. Hormones also dete mine the formation of l!owers, sterns, leaves, dv awdbioe of l a es, and the development and ripening of fridi. Plants, unlike animals, lack gl nds that produce and se ee hormones. Instead, each cell Is capable of producing hormones, Plant hormones shape the plant, effecting seed growth, time of flowering, the sex of flowers, senescence of leaves, and fruits, They affect which tissues; grow upward and which row downward, leaf formation and ste growth, fmh development and ripening, plant longevity. ;:·:0 evers. plant death. H rm nes are vdal to pk t growth, and. lacking them, plants woud be m sih a nws of rmdlfi enviated eeds.
hi one embodiment >>,·<· discosure wiaws to ba erial strains, compositions thereof, and methods that modulate- the production of plunt rowth hosyoones, hi one embodiment, the disclos re relates to bacterial strains,, and compositions thereof thai increase the production of plant growth hormones.
Irs one embodiment the disclosure relates to Pa, on ml strmray composidons thereof, and methods that contain genes thai modulate the product ion of plant growrh hormones. In one embodiment, the discl sure relates to bacteria! -mo s and compositions there f* hat contain genes Ueu increase the production of plant growth hormones,
L .- u ms
Auxi are compounds that positively Ini!eetwe cell enlargement, bud ibm tioty and root initiation. They also promote the production of other hormones and In cosywrsotion with cytoki ns, they control the growth of stems, roots, and fruits, and convert stems into flowers,- Anxies affect cell elongation by altering eel! wall plasticity. They smrnn!ate ca bi m, a snirrype of menstem cells, to divide .nr.! in stems eanse sec ndar xylem to dnAaemrm:
Auxins act to inhibit the growth of bods lower dene the stems f spies! dominance) and also to promote lateral mi adventitious root development and growth. Leaf abscission is Initiated by the growing point of a plant ceasing io produce a cams Auxins '·■■■■ seeds regelate specific protein synthesis, as they develop within the flower after pollination,, causing the flower to develop a fruit to contain the developing seeds. The moat common auxin found if; plants Is iodoie-S-aoetic acid or !AA,
In one emb dim nt the dis losing relate to bacterial strains, compositions thereof and mehods that modulate the production of auxins. In one embodiment, the disclosure relates to bacterial strains and compositions thereof that nx rease the production of auxins. In o< -s e bodiment*, the dkclostste rekka to bacterial sereins, eemjwWoiw thereof end methoek that contain genes that modulate the production of a>r ms. in one embodiment, the disebsnre eeiates io bacterial strains, and compositions thereof that contain gen«s thai increase the production of auxins. in a embodiment the disebsnre relates to bacteria! strains thai contain the 2,3 - butanedio} deh drogenase gene (bdhA) and c m s ons ibaorof that modulate the ro uction of auxins, in one embodiment, the disclosu e relates to bacterial strains that contain the bdhA gette, and com ositions thereof that increase the production of auxms. The bdhA gone is associated ·.· ah production of , bntandiol a volatile compound associated with pJan* aux in prodoe on and regulation.
In one embodhncnp the disclosu e rs? cs to bacterial strains that modulate the production of 2,3 btnandlo! cab compositions thereof In mse embodlmemy the diseiosrjfe relates to bacterial strains that modulate the production of 2, butandioi and compositions thereof is? another embodim nt, the bacteria! strains, compositions, snd methods disclosed hete can increase the am u of auxin in plants, in o-ne embodiment, au i in ptertts is increased by a percentage selected from the group consisting of horn 1 ¾ to 3%, from 3% to 53k from 5% to 7%. from 7% to 9%, from 9% io 1 1 %, from 1 1 % to 1 3%, from i 3% to 15%, from 15% to 1 7%. boot 17% to 19%. from 19% to 23%, and g:r¾tter than 25% as compared io an untreated control,
in another embodiment the bacterial strains, coaipesiilomp and methods disclosed herein can increase expression of the bdhA gene to plants. In one embodimen expression of the bdhA gene in plants la increases! by a percentage selector! from the group consisting of from 1% io 3%., from 3% to 3%, l¾orn 573 t 7%, from 77;, to 9%>. front 977 to 1 17k from I i % io i 3%, from i 3% to 15%.. fvM ] 5% to 37%, from I 7% ro 1 %, S:i¾ni 1 % o 2577, and greater thai: 2S% as compared to a untreated control.
In another embodiment the bacterial strains, compositions, and methods disclosed herein ears increase 2,3 b tarsdtoi in plants. In one embo iment, 2.3 bntarrdlol concentration in plants Is increased by a pe; c . n;,¾e<- selected from the grt p eorssistmg of from 1 % to 373, from 37¾ to 533. from 3% to 77¾, from ?9¾ to 973, from 937 to I i7s, p«m Π % ίο 1 7k thorn I *'" ·, k l 57¾%roro k% m 17%, iront ! 73¾ to 97 ,. front !9¾ to 23%, and greater than 2$% as eomptaxid to unkeated conotof
in yet another embodiment, the dkelosnte relates te methods to Increase production o plant ations, in one embodirnenf a method to increase pi-oductioo of plant attains comprises applying at least one bacterial strain co rising the bdhA gate to a seed, a plant, or plant pari, in another embodiment a method to increase production of plant aux ns contprises applying a composition comprising at least one bacterial strain comprising the bdhA gene to a seed, a plant or plant part, in still another embodiment the method comprises applying the bacterial strain or composition to a seed, a pla or plant part in sod. In another embodiment the composrhon f ther comprises one or more agrochemscal iy active compounds . in another ewitedmefit, a method to mensaae ptrasogio of plam anstlns eompilses sp ing ss one ;·>:.ί·;Κ·ίί.·· strain that produces 2,3 batandb! P: a se d, a pbau, or plant par. In another em odiment, a method ro so,. Γ·Λ Ο.'· ^-doen · ; of plattf atr s costumes a pl i a composition comprising m fege · an- bacted&l strain that produces 2,3 butes-sd ! So a seed, s plant or lant parr. In stiU another embodiment, th¾ method comprises applying the bacteria! strain or composition to a seed, a plant or piaat. pari in s il In another embodiment the composition further comprises om or more agrochcmic&ii active compounds.
In one ernb djrnenp ihc bacterial strains that irtodmate produedot of pbmt auxins a e Aorn/sa oio ao ipn ro/iOis Irs one embodiment the Α< ·:··νΙ.ο vO-ahrs drat modulate production of p m anv. s are selected from tbc group consisting op Bottoms yioBqiq/Bck S2 ' Bacillus m 'hiiqulfac>i< BS205 Bacillus
Figure imgf000083_0001
3 A PA Bad/it,'.* ar:;ykHiq fcsi~ 'n LSSA L isA/fea nyk q Ja h? ABP2 8, dmbt- n;y >kqui†*ci s 22 FI . Batik w
am h qmfyilent Bblas snd eombmahons thereob
Iti one envbochmenf the baensrial strains t at contain the bdhA gene arc Bactiim
a ykkiqk/iicien . In one esnbodbneM, the bacterial strains thai contain the bdhA e e are selected isorn the group co s ti g of: Ba-kttxs *!?qkBqnifi?ckrii FA"? BaclU mnyloi jtifbcem S2b84. BaciMi.<s nnioi! i<ijh iY>i'i 5 AP4, Ba Bha amyiikiqukaci s 3 A A ¾oAms■
Figure imgf000083_0002
LSSAOf :···.·· Avn iitn hti iiifh BP278, BacBI -;mykikqdfack:<is 22CPL and Aocther a B}lBpBB;cie><x BSI8, and combinations flnensof
a. mdolp- - e ¾ Aed
huiose- -acetic acid f!AA) I;; the most' a m, t;aturaily--oecumng, piant hormone f the auxin class. Chemically, lAA is a oarboxyhc aeld in hich die earhosyl p is attached through a methylene group to the C-3 position of an indole ring.
lAA is predominantly produced in cells oidhe ape>. (bod) and very yoang leaves of a plant. Plants can synthesize lAA fey several independent bmsynthetie adi a . Four of them star! from tryptophan, bra iacre is also a blosyntbetio pathway Independent of tryptophan. Plants msiniy produce IAA item tryptophan through indolc-3-pyryvlo acid,
I.AA hat many different effects, as all au ins do, such as indneing cell elongation and ee-i division with all sobsecpsefit efirnts tor plant gm th and development. On a larger stale, IAA servos ns .dgaabag moiecnle ncecssar)' ibr developmeni ot piant organs and coord!nahoo of growth.
Figure imgf000084_0001
in ne embodnneot, the disclosure relates m bacterial steams, compositions thereof, nd methods that produce IAA. in one embodiment., the disclosure relates io bacterial strains and compositions thereof that produce IAA vitro. In one embodiment, the disclosure relates io bacterial strains find compositions thereof that pr duc ΪΑΑ i the resence of tryptophan.
Io another embodiment, the bt&ttrfal srains, compositions., and methods discl sed erein «a« m crease IAA in plan , i one embodiment IAA in plants is i creased by a percentage selected from e roup consisting ot from ;% to 3%, from As ···.> 5'So, from.5% to 7%. front 7¾ t 9%, from 9% to I ] %, iron": I ϊ % to i ;¾., front ] 3% to i SoA from i 5% to 17%s from 17% to 19%y from 10% to 7 ¾A and greater than 25% as compared fo an untreated cootrol.
Its one embodime t, the disclosure relates to a method o incre se pm^dtsction of plant auxins comprising applying at bast one bacterial stran that rod s IAA to a seed, a plant or plant pint. In another embodiment method to increae production of plant auxin comprises applying a composition comprising at least one bacterial s ain that produces I AA to a seed, a plant, or plant past, in still another emb diment the method comprises applying the baetenal strain or composition to a seed, a plant or plant part in soil. In another emtxxtimes.iL the composition further' comprises one or more agroc emicahy active compound
in one embodiment, the haetedal strains that produce IAA are selected from she group consisting of: !?om/0rs wyioiiQ ifhek s, .Aos-Vm oe/mo; Ae A, < d Bof: i?;s s bmits. In one embodiment me bacterial strains that produce IAA are selected front the group c nsisting on AcmA:- (>r:\ Am -ma c ma-- 8A20¾A ty .;;-/o.,. amyioijitifitef s bSSAOI, Bacilim ei!ny liqtf!/aeimx I013:. and combinations thereof. in one embodiment, the bacterial strain that produces IA is BeeiH iichemfamk L2I.
In still another embodiment,, the bacterial strain that produces !AA is Bacillus -ubdlis 3BPS. in one <- :id. ':.jir !■-·«;. the diselesnre relates to bacterial strains, compositions thereot and rrrethods that contai a gene that encodes tryptophan synthase, ίτρΛΒ, which is the precursor for mdoic~3 -acetic acid (lA A) production,
in another embo iment the disclosure relates to hastens! strain;;:, compositions ere and methods that produce t y an synthase.
hi another embodiment, the bacterial strain?, compositions, and methods disclosed herein can increase expression of the ey. tP- gene in lants, h; one . 0%. hncnr expression or ' the ϊ.ψΛΒ po e as plants is increased by a pereeninge selected from the group consisting of from i % to 3%, from 3% a. 5%. front 5% to ?%, from 7% 9¾, irons 9% to ! 1 %, born Π % to ! 3Hf firom 13% to 15%, from i 5% to i 7 , from ! 7% to 19%, Irons 19% to 25%, and greater than 25% as s-psivb to an untreated control,
in another embodiment, the bacterial ste ns, compositions, and methods disclosed herein can increase the amount of tryptophan synthase in ptatts. fai one embodiment the amount of tryptophan synthase in plants is increased by a percentage selected fro i the group consisting of from 1% to 3%, bom 3% to 5%, ir m 5% so 7%, from 7% to 9%, from 9% to I i %, m i ! % to 3%, from i 3% to 15%, fro; a ; 5% to 17%, h'orn 17% to 19%, from 1 % to 23%, and greater than 25% as compared to an ent eated control
in one embodiment, the isclosure relates to a method to increase production of plant assarts ii rtt riy rig apply ing at least one bacterial strain thai contains a gene that encodes tryptophan synthase to a seed, a pl nt, or plant part, in another embodiment a method to Increase production of plant auxins comprises applying a composition comprising at least one bacterial strain that contains a gene that encodes tryptophan synthase to a plant or plan? par . In still another embodiment the method comprises applying the bacterial strain or composition to a seed, a plant or plant part in soil, i'o anoiber embodiment, the composition bather comprises one or tnore agroeherolcalSy active compounds- In another embodiment, the ores ansin s 1AA, in one embodiment the disclosure relates to a method to increase production of plant ansdns comprising applying at least one bacterial straio that oduces tryptophan synthase lo a seed, a plant or plant pan:, In another embodh-ncnp a method to increase r ducti n of plant auxins comprises applying a composition comprising at least one bacteria! straift that produces itc tephan synthase to a la t or plant part- in still another embodiment the method comprise applying she baetenai strain or composition to a seed, a plant or plant pari its sod. )n another embo ime t the composition bather comprises one or more sgroehemica!ly active compounds, in another embodiment the plant auxin is IA A .
in one emhodirmmt the bacterial strains thar contain a geae that encode nyptophan s n1.h3.se are Ba iii an;y/otiiiikfhciet?i. In one embodiment, the bacterial strauts ring contain a gene tha encode tryptophan s nthase are are selected from the group consist ins, of: Baciiim myioikp f kns ES%o Bacil!m Luuyioiiquifyckm 8S20S4, BacBins em?yk}i:qwfaek>t cot 7% Bact!! m) vkg c ^ /u%#% teeife
Figure imgf000086_0001
LSfbyob r¾¾of/os
Sl , Bgci!im a yhlkpqfack ,d.o/% ¾ and eo ionaiions thereof
C, Promote Root Ffcfsnafioft
The root the organ of a plant: s½ typically lies belosv the surbboe of the soil However, roots can also bo aerial or aerating (grovclng op abo e the ground or a- ;.v. ; dh a ov ··■·, ,Ο'·.Ο '·. ITaihermore, a stem normally occurring below ground Is not exceptional either (see rhissome). Therefore, as used herein, the mot is the won-ieal oon-nodes bearing arts of the plant s body.
The first rooi that comes from a a is called the radicle. The four major funct ions of fo ts are: Π ) absorption -n w.u., - and inorganic nutrients; (2) anchoring of the plant body to the ground and supporting k: ( a) storage of food and oao'i-o a> and {4) vegeunave reproduction . Roots often un l sen in storage of food and uaneato The roots of most vasetdar plant species enter into symbi i i its cenain fungi to form mycorrhisas, and a large sng-,. of otber organisms Including bacteria also closely associate with roots,
in one embodiment, the disclosure relates to bacterial sir¾;ns. c mp siti ns thereof and methods that promote root ionnabon in plants, in one embodiment, die disclosure relates to bacterid strains, eon-positions thereof, and aiethods that stimulate root format ion in pianos, hi one embodiment- the discl sure relates to bacterial st ains, c m sit o s thereof, and methods that Increase the strength or vigor of roots In plants. In still another em odiment the disclosure relates to bacterial strains, compositions thereof and methods that increase root mass in plants.
In one embodiment, the disclosure relates to bacterial strains, compositions thereof and methods ih&r increase toot fonctioa in lan ?* including but not limned to: absorption of water nd inorganic nutrients, anchoring of the plant body to she ground, a d supporting it, stora e of food and nutrients,, and vegetative reproduction,
i.o another embodiment, the bacteria! strains, compositions, and methods disclosed herein car. Increase the amount of root formation in plants. In one embodiment the amount of root formation in plants is Increased by a percentage selected b m t e group con istin of Irom 1 % to 3%, from % to ,5¾. from 5% to ?¾t ass a 7% to 9%„ fr m 9% to 1 1 ¾, thorn 1 1 % to 13%, b¾m i 3% to 13%, fixjro 13% to I 7%, from 1 % to } 9%, f sm 19% to ,- S': .,, iOate than 25% as compared to an untreaied control
Its another embodiment, the bacterial strains, compositions, and methods disclosed herein can increase root mass in plants, bt one em od ments root mass in lant is increased by a e centage selected tfom the gnaup consisting of from 1 % to 3%. bom 3% to 5%, f n-; y¾ to ?'%, from 1% to 9%. from 9% to 1 1 ¾, Sk>m I : % >· ·· I 3%, from 13% to ) 5%; sVom } $% to 1 3%, h¾m 17¾ to 19%, i: ,ο · 19% to 23%. arid greater than 25% as compared to an untreated control in another embodiment, rhe Haete«½i strains. 60«*}> sUk>« a d meth d* d sctossd sreff? can increase the strength of roots in plants, in one embodhnent, the strength of roots m plants is btereased by ¾ percentage elected from - e group consisting of ( rn 1 % to 3%, fro 3% ¾%; fhs 533■· · 7%, from '?% to 3b% from ¾ to I i %, ih>m Ϊ 1 % to ί 3%, bom 1373 to 1 S%, horn j 5% to l 7%, from i 7% tc 19¾, jhsm i bps to 25%, and greater ihan 25% as compared to an nntreated control,
in an ther embodiment, she bacteria! smainsy compositions, and methods disctesed herein can merease root ftmeiksn in plants. In one embodiment root function in plants is increased by a petcentage selected from the group consisting of from 1 % to 3%, from 3% to 33b, from 5% to 7%, ben e 733 to 9 , h a: 933 so 1 ! %., b m U % to 33%, from ] j% to i 5%, from i 3% to I ?%, thorn I %3 to i ¾t from 19% ro 25%, and greater than 25% as compared to an animated control.
In one embodiment the disclosure relates to methods to promote root formation in plants, to n embodiment, the method comprises applying one or more bacterial strains or eompesi'tkms thereof to a seed, a plant or plant pad in n effective amouni to promote root formation.
In one enmodirnent, the disclosure relates to methods ro stimulate root formation in plants, in one embodiment, the method comprises applying One or more bacterial strains or compositions thereof to a seed, a plant, or pbmi part in an effective amormt to stimoiaic root formation.
In one embodiment, the disclosure relates to methods io increase root mass in plants, in one em odbrse n, the method com ses applying one or more acte ial strains or compositions thereof to a seed, a plant, or plant part in an effective amonm io increase root mass in plants.
in one embodiment, the disclosure relates to methods to iresrease the strength or vigor of roots o plants. In one embodiment: the me d comprises applying one or more bacterial strains or compositions t ere to a seed, a plant, or plant, pari In an effective amonm to increase the strength or vigor of roots. hi one embodiment, the disclosure relates to methods to increase root function in plants. In one embodiment, die method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or pi are part In an eiiesbve amount to increase root fancilon .
I , Marigolds
In one embodiment, the seed, plant, or plant part described in Section C above Is a marigold, in another embodiment, bacterial strains, siomposiiioos thereof, arid methods that promote root fbrraetiwt rose smnulation, erease the strength or vigor f the roots, or increase root function in a marigold are selected ova a the group consisting of: Bacillus io/iquifac ns, Ba illus lick nifor i-;, and
combinations thereof.
In one embodiment, the bacterial strains, compositions thereof, and methods thai promote root formation, root stimulation., merease the strength or vigor of the roots, or increase root fun doo a marigold are selected from the group consisting ot: Bacillus m yh!iquifyo^ 7233' i . Bacill s $Φ ίίφ ϊ ηϊΜ AH., toemhit m iί i tH si 3 A N, loeiftoi
Figure imgf000088_0001
am loiiquiflicie BS ! S, B ciihis ifck&nifor s 8L2 L and combinations thereof.
In another embodiment, a composition comprising Baeiikis cmyloiiqiqf cie 22CP I atal I 5AP4, and methods thereof promote root fonmtt om root stimulation, increase the strength or vi o of the roots, or increase toot function in a marigold.
2, Rmt i& tim in omatoes
In o is embodiment the seed, plant, or plant part descnbeej in Secti n€ above is a tomato. In anothe embodiment, bacteria! strains, compositions t ere f, and meth ds that promote root fbraiadoro root stimulation, increase the strength or vigor of the roots, or Increase root function in tomato are selected from the group consistmg of; ciH
Figure imgf000088_0002
and B dihi* ikhswfi>r i&t sad combinations thereof;
is ί«·¾ο embodiment, the bacterial strains, co sition thereof: sod methods thai promote; root formation, root stimulation. Increase the strength or vigor of the roots, or merease root function in a tomato are selected from the group consisting of. BocHhis v yioHiHii/acie 2014, actikxt
Figure imgf000088_0003
Bacillus li ke formls BL2 I , and combinations thereo
! , Promote S mt Wmmrntimt
As used herein, the term "shoot" refers to the serial portion of a plant Ineleomg the stem, ranc h;- ·· and leaves, The term "shoot" may also refer to now. Immature growth of a plant. The shoot of a plant has many dmci ns including but not limited to photosynthesis, reproduction and dispersal, and food and water eondocbon.
In one embodiment the disclosure relates to bacterial strains, composhions thereof, and methods that promote shoot formation In plants, in one embodiment, the disclosure relates to bacterial strains, compositions j hereof, sad methods that Increase shoot height in plants. In yet another embodiment, the d!sclosstre relates to bacteria! strains, compositions thereof, and methods that increase shoot w dth m plants.
In one embodboeag the disclosure relates to bseseria! strains, eompositiena thereof, and methods that stimulate shoot ftainstior? in plants, in one embodiment, the disclosure relates to bacteria! strains, compositions thereof, raid methods thai increase the strength or vigor of shoots In plants.
In one embodiment the disclosure relates to bacterial strains, compositions thereof, and methods that increase shoot fenctiots In plants including but noi limited to; photosynthesis, reproduction and d!spersab and food and water conduction.
In another embodiment, the bacteria! strains, compositions, and methods disclosed herein can increase shoot height ift plants, in otic eml?odfrneat, shoot heig In plants Is Increased by a. percentage selected front the group consisting fr m 1% to 3%, from 3% to 5%, front 5% to 2¾, from 7% to. n%, dons ;- ·> >·> t ! ¾ f m 1 17a to i 3%%Vosn 15%, from 15% to l 7%%Votn \ to 191% from l %¾ So 25* and greater than 25% as com are to untreated control.
In another embodiment, the aeter stfgfes, eooipositlonsy and methods dis losed herein can increas shoot width Irs plants. I on embodiment shoot - widt in plants as Inesasaaed by a percentage selected from the creep o sisting of iron; 1 %. to 3%, tVom 3% to 3%. from 3% to ?¾, iront 7% to 9%, from % to i %% from } ! % to 1 3%; from : 3¾ to 1 5%, b m 1 % to 17%, h orn j ?% to 19%, hem 9% to 25%, and greater than. 25% compared to an untreated control.
In another embodiment the baetesial strains. compositions, and methods disclosed herein can increase the ·αη· ···· .< of shoot formation in plants. Its one embodiment, the amount of shoot formation is plants is Increased by a emsiit ge selected foam the group consisting of b om 1 to 3%., from 3% to 5%, from %¾ to 7%5 from 7% to 9%, from 9% ¾. ! I %, i ran \ I % a 13%, from 1 3% to I 3%, from i % , to 1 7%, f rom 17% to 19%, mam 19% to 25%, arid greater than 25% as compared to an untreated control
in another embodiment the bacterial strains, compositions, and methods isclosed herein can ios re -a the sbength of shoots a -: plants. Irs one embodbnem. th¾ strength of shoots in plants is increased by a percentage selected irons the group consisting of from i % to 3%, from 3% to 5%, from 5% to 7%, iron? 7% to 9% from 9% to 1 1 %, from 1 1 io ί .3%, from 13% to i 573. from 15% to 1 7% from I ?%. to i 9%, from 1 % to 25%, and greater han 25% as compared to an untreated control
In another embodiment the bacterial strai s,, compositions, and methods disclosed herein can increase shoot function n plants, io one embo ime t shoot bmetlon in plants is increased by a percentage selected from the group consisting of ' from 1 % to 3%, from 3% to 5%, h¾m 533 to 770, from 7% to 9%, from 9% to 1 13b, from I % so 13%, from 1 % to 15%, f om ! 5% to- 1 733, from 1 7% to 1993, bom 1 % to 35%, and greater than 25% as eompsaed to an untreated control
In one embodiment, the disclosure relates to methods to promote shoot ftmsatlon in plants. In one embodiment, th method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in art elec ive amount to promote shoot formation.
in one embod iment the disclosure relates to methods to increase shoot height in plants, fa one emb diment, the method comprises applying one or more bacterial sfnons or compositions themof to a seed, a plant, o plant part in an effective amount to inooaa.se shoot height in plants,
in one embodiment, the disclosure relates to methods to Increase shoot width in plants. In one embodiment the method comprises applying one or more bacterial strains or compositions, thereof to a seed, a lan or plant part in an ef ective amount to increase shoot width in plants,
in one embodiment, the disclosure relates to methods to stimulate shoot formaiioii in plants, in one embodiment he method comprises applying one or more bacterial s rains or compositions thereof to a seed, a plant, or plant part in an cfleetivc amount to stimulate shoot formation. i one em sd -oeat, the disclosu e relates to .oomod;. m ίίκ-;·;,»? mo stioogi r vigor of s ts io plants. In one enfbodhnem. ihc method comprises a pl ing ne or more bacterial strains or compositions thereof to s seed, a plant, or plant part in defective amonot to increase the strength or vigor of shoots.
In one embodimetrt, the disclosure relates io meth ds to increase? shoot function in plants. In one csdvs iiO ro the method c mprise applying one or n, ····;·:.· baeten l strains or compositions thereof to a seed, a plant, or piasn port so an effective ism nni vo incs'ease shoot function,
1. ftrig ld*
In one emb din?o L she seed, plant, or plant od described in Se ti n f> ab ve is a msrigoid, in another embodiment, bacterial str& s, and compositions thereof that promote shoot formation, shoot stimulation,, increase shoot height, incre se shoot width, increase the strength or vigor of the shoots, or increase, shoot fsmerion in a marigold :ae /fa odo m faik iif exs.
in a other embodiment bacteria! strains, and compositions thereof that pros-note shoot formation, shoot stimulation. Increase shoos height, increase shoos wi th, increase the strength or vigor otohe shoots, or increase shoo- function in marigold are selected mom the group consisting of: /be ; ·;·.
ο,·· s ·'■ '0-g;//iO-f as 22CP , /¾oo ¾> ,·;ην:·¾;,θο, .·..···.<> 15 AF4, :-S .¾..·· myhi;< i& i i US I 8, and c naio s thereof
In yet another embodiment, a composition comprising B c hts
Figure imgf000090_0001
and iSAI promotes shoot formation, shoot stimulation, increase shoot height, increase shoot width, increase the :--oeroh. or vigor of the shoots, or increase shoot inaction in a marigold.
In another embodiment, a composition eong:nbsmg .B«c//f¾s amyia!!qtifhei ii* 22CPI and BSIS promotes shoot formation, shoo; stimulation, lnei¾ase shoot height, increase shoo? width, increase the strength or vigor of the shoots, or increase shoot function in a marigold.
2. ! Von a toes
in one embodiment, the seed, plant, or pkrss part esexbed to Section fJ above. Is a tomato, In another embodiment, bacterial strains,, and compositions thereof that promote shoot formation, shoot stimulation, increase shoot height, increase shoot width, it5crease the ste th or vigor of the shoots, mcrevrce shoot fooeoon its a tomato are selected horn the; group consisting of: /fo. ;·¾.·,
Figure imgf000090_0002
Buetf! s iickefufbnnis, Px tiibacfHus poiy yxa, and combinations thereof
it? another embodiment, bacterial strains, and compositions thereof that prosrsote shoos formation, shoot stimulation, increase shoot height, increase shoos width, increase the strength or vigor of the shoots, or increase shoot function in tomato are selected from the groap consisting of: Bacillus
i Mi tiif eJie .ο 2 ο 3adHt{s amy liqyifbciens M84, B eui s wioHqfiifadms 22CP1, eiciHus mny ! dfyc is i 5AP4. B dihs
Figure imgf000090_0003
3 Af 4, M&eil'his a yioiiquifackm LSSAO 1 , Sac'ihis e ylotiqu a tu ABPi g. %,,sbdss a k i&. f&i>ims ¾S I S, Bacik ikke?-tkrmk BA84 , Bii if'us f heriifb fsiis BL2 L Pa wbaciitus oiynsyxa A SP ) §6, and combinations thereof.
E. Lesi Lea b
A leaf is Signed to cappme sunlight which the ant uses to nmk§ food th wgb it process called photosyrnhesis. Most plants' food is made in t s ·. . · l ave . Leaf lengths, v - widely from one plant species to the next. In addition, leaf lengths oat vary within a single plant.
In one embodiment* the disclosure relates to bacterial strains, compositions thereof, and methods promote leaf formation in plants In one embodiment, the disclosure relates to bacterial strains, compositions thereof, and methods thai increase leaf length m plants. In yet another embodiment, the disclo ure relates to bacterial strains, composition? thereof, and methods that Increase leaf width in plants.
!n one embodiment ie disclosure relates to bacterial strains, compositions thereof,, and methods that stimulate leaf foruod ion in plants. In one embodiment, the disclosure relates to bacterial strains, compositions thereof, and methods that Increase the strength or vigor of leaves in plants.
In one embo iment, the disclosure relates to bacterial strains, compositions thereof* and methods that increase leaf fanci ion in plants including bat not limited to pk>kawmhesis, reproduction and dispersal, and food and water connection.
In another embodhrsent, the bacterial strains, compositions, and methods disclosed herein can increase leaf length in plants. In one embodiment, loaf length in plants is increased by a percentage selected from the co -op consisting of from f% to 3%, from 3% to 5%, from 5% to "o , from 7% to 9%, front 9% to ! 1 %, from J I % to 1 3%, from 13% io ! 5¾„ from 15% to Γ< . irons 17% Pa 1 %, from 19% to 25%, and greater than 25% as compared to an onneated control.
in another embodiment the bacterial strains, compositions, and methods disclosed he-rein can increase leaf width in plants. In one embodiment, leaf width In plants is increased by percentage selected i on ! the rou consisting of from 1 to '}%, from 3% to 5%, from 5% to 7%, io -n - ?% to 9%, from 953 to 1 1 ¾, from i I % to 13%, from 13% to 15%, from 15% io 17¾, from 17¾ to i 95% Irons 19% to 25%, and greater than 25% as compared to an nntreated control
In another embodiment, the bacterial strains, compositions, and method disclosed herein can increase the amount of leaf formation in plants, in one embodiment, the amount of leaf formation in plants is increased by a eiWit ge selected from the group consisting of from 1% to 3%:, front 3% io 5 . from 5% na 7%, from 7% to 9%, from 9% to ϊ 1 3, Irons I ! % to 13%, Pom 1.3% to 15%, from i 5% to !?%, from 17% to I ->%.. 19% to 25%, and greater than 2553 as compared to t untreated control.
In another embodiment, the bacterial stratus, compositions, and methods disclosed herein can Increase the strength of leaves in plants, in one embodiment, the strength of leaves in plants is increased by a erce tage selected from the group consisting of from 1% to 3%-, u- -m >% to 5%, fro S% to 7%, % -m 7% to 9%, imn; 9% to i ] %, ■■·■:■. I ) % to 13%, test! I % to 1 S . bom 1 $% to 1 %% ftmo 1 %¾ to 19%, boons 1 o% to 25%, a d greater than 25% as eompaosd to an untrea eontg:>f
In a her embodiment, the bacterial sprains, sompnsliions, and methods obselosed herein ear* increase leaf function in plants, in one emb di ent, leaf function in plants Is increased by a percentage selected frotn Pie group consisting of fPom 1 % to 3%, from 3% to 5%. from 5% to 7%, from " o.- to 9%, from 9% to Π from I 1 % >· · 13%, from 13% to 1 %.. iron 15% io %%, from %% io 19%, from 19% to 25%, and greater than 25% as compared to an untreated control.
In one cmboihmem, the disclosure relates to methods to promote leaf formation in plants, hi one embodiment, he method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant o plant par m an effective anton to promote leaf formation.
In one embodiment, the disclosure relates to methods to increase leaf length in plants. I one embodiment, the method comprises applying ono or more bacterial strains or compositions tlmreof to a seed , a plant, or plant part in an effective amount to increase leaf length in plants.
In one embodimem. the disclosure relates to methods to increase leaf width hi plants, in one emb di ent, the method comprises applying one or more bacteria! strains or compositions thereof to a seed, a plant, or plant part in an effective amount to increase leaf width in plants.
I one embodiment, the diselosnre relates to methods to stimulate leal formation in plants, in one ennbodlrnem , the method eomprisms applying one or snore bacterial stra ins or compositions thereof m a seed, a plant, or plant part in an effective amount to stimulate leaf formation.
in one embodiment the disclosure relates io methods to Increase the - o enrol: or vigor of loaves in plants. In one embodiment, the method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in an effective amount to increase the strength or v igor of leaves.
In sic embodiment, the diselosnre relates to methods to increase leaf "function in plants. In one embodiment, the method comprises applying e or more bacterial strains or compositions thereof to a seed, a plant, or plant ri In an eiieetive am un to increase leaf function.
L fcfsrige!ds
in one embedhrmnt, the seed, plant or plant part described in Section E above ts a marigold In another embodiment, bacterial strain , and compositions thereof that promote loaf formation, leaf stimulation, increase lest length, increase leaf width, increase the strength vigor of the leaves, or increase leaf function in a mangold ·ην i'omfoo ooo ό ·ό.;ν.;0Λ /en -
Figure imgf000092_0001
and oombbmi ions thereof
fa another embodiment, bacterial strains, and compositions thereof that promote leaf formation, leaf stimulation, increase leaf length, increase leaf width, increase the strength or vigor of die leaves, or increase leaf function in a marigold are selected from the group consisting of; Bacillus my!oiigitifacienx 22CP L «·?/·/,.·; tti iquifa tefis ISAM, ftecithfS ^httqtiijhce LS$ AOL
any liqiifv -iVi BS!S, Ι'^^ί-ί-ακ ·,Ό;., .w,Ywco:.; ABPS66. and combinations thereof
2. matoes
IB one embodiment, the seed, plant, &r plant pm described in Section D &h&vv k « tomato. !« anothese«dx>dimem. hseteri&l strains, and compositions sbereof thai omote leaf formadon,. leaf stimidatlon. Increase lea length, increase leaf widt , increase the strength or vigor of die lea s, or Increase leaf function In a tomaio a t selected om the -wasp oonsistmg ot: i aswm i<< yk--iiqv/ c '. Backi &dwn /0mrfp f ba liiifs poiy yxa, and combinations thereof.
In another embodiment bacterial strains, and composii n thereof thai promote leaf formation, leaf stimulation, increase e f length, tnc-rease leaf width, increase the stren th or vigor of the leaves, or increase leaf I .naction in a tomato are selected rom the group consisting of; Bad/lus yloli /fh kfis BS27, Badik inyl ikikfa Boc/te awykkiq ifivke d b'sb Ikiciihis myh!iqikfac s LSSAOK Sooiiiv ^!diquifack s\BP278, Bacikus amy!oHqniJ c BSI 8, Bac l in i iformk BAS 2, S iHi ikh^mfbrmk BL2 \ ; i^mbacHius pofymyxa ABPI 66. and combinations thereof,
F, Flower Bud*
Flowers are the reproductive part of oost plants. Flowers contain p-d!en and tiny eg sailed ovules. After pollination of the flower and fertilization of the ovule, the ovule develops into a fruit. hi one embodiment, the disclosure relates to bacterial strains, compositions thereof, and methods that promote flower bod presence i plants, in one embodiment the disclosure relates to twererlal streams, cornposiUons -1 :··.·. moo and methods that promote bower bad tbrmaoon in pianrs. in oae embodiment, the dlsolosnre rehnes to bacterial su¾l?rs„ com ositions thereof, and methods that increase the numbe of flower bods in plants. In yet another embodiment, the disclosure relates to bacterial straios, co positlorss thereof, and methods that increase the size" of flower hods In plants.
In one embodiment, the disclosure relates to bacterial sbains, compositions thereof and methods that siintuhte flower bud growth In plants. In one cmbodunent,, the disclosure relates to hacieriai strains, compositions thereof, and methods rhat increase the strength or vigor of slower bads i piarns.
in orse eoibodiment, the disclosure relates to bacterial strains, compositions thereof and methods that increase flower bud function in plants ncluding but not i united reproduction and dispersal.
In another embodhysene the bacee ial strains, compositions, and methods disclosed herein can increase the number of flower bods in plants. In one embodiment, the number of flower bods in plants is increased by a percentage selected from the group consisting of from 1% to 3%, from 3% to 5%, ifom 5% to ?%. If om 7¾ to §¾, from 9% to 1 IH.from 11% to 13%; Item 13% to 15%, from 15% to 17%, from 17% to 1 %, from 1 % s · 2s¾,, and reat r than 25 as compared to an untreat d e mr !. In another emheejimem, he hgeiebai sbtbns, eo oah ss., and methods rllse sed herein can increase the siee of flower bads in phmts. in one embcdirnent he slae of flower bods in plants is cmaaed by a pvu'<oa.;;;.:o selected from the g oup consisting of fr m \ % to a%; irons 3% to 535, from 5b!, to 7%., boa! 7% to 9%, from 933 to 1 ! ¾. beta 1 \ ¾ to 1 3%. b oa . 1 3% so 1 5%, fro*-: 1595 to 1 73b if >·; · 1 7% to 19%, boo; 1 3b to 25%. and greater than 25% as compared lo an untreated control.
Is another enmwh oo . the bacterial strains, coiy osibons, and methods d sclosed herein can inc ase the amou t of -flower bud formation in plants, in one embodiment, the amount of flower bud Isa-madon n plants is Increased by a percentage selected item the group•••'m .mnp of from ) ¾ to 3%, from 3%, to 5 , from 5% to ?%? from ?% to 93b, from 9% te ί i 3b b om I ! % to i 333, bom ί 3% to 1 5* irons I 5% a ' l ?9b !rotn i ?% to 1933. front 1993 to 25% , and greater than 25% as compared to an entreated control
In another embodiment., the bacterial strains, compositions, and met o s disclosed herein can increase the strength of bower buds in p nis- in one embodiment, f.h« strength of fese bads In plants is moi-eased by a percentage selected f om the group consisting of drotn 195 to 339, from 395 to 5%, from 5% to 7%, from 793 to ff % t'roni 95¾ to 1 1 %, bom 1 ! 93 to 1393, from 1 393 to ! S95, from ) S% to ! ?¾, bunt 1793 as 19%,, from 19% to 2593. and greater than 3593 as compared to an. untreated control.
la another embodiment the bacterial strains, compositions, and methods disclosed herein can increase flower fend function in plants. In one embodiment* flower bad function in plants is ntcreased by a percentage selected bom the group consisting of from 133 to 35b iron; 39¾ to 5%., from 5% ·. · 7 , nvn: 7% to 995, bom 935 io 1 1 %, from i 155 to Ρ39¾, boas 33% to 1 5%, from i 55%o 1795, from ! 790 to 1955, from lab s to 2535, and greater rh a; 2595 as compared to ;m untreated comrof
In om embodiment, the disclosure relates to methods to promote ilower bud formation. In plants. In one embodiment the method comprises a lyin one or more bacterial strains or compositions thereof to a seed, a plant, or plant pari in an effective amount to ροη:·· ·η· Ilower bud fonyabion.
!n one embod iment, the disclosure relates to methods to Increase the nanaber of flower buds in plants, in one embodiment, the method comprises appl ing o e or more baei«ri»! strains or compositions thereof to a sees! a plant, or plant part in an effective amount to Increase the number of flower buds In pkmts,
tn o embodlnmat, dm disclosure relates to meth ds to increase the sme of flower buds In plants. In one embodiment, the method com rise applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in m effecti ve amount to increase the size of flower bads in plants.
In one embodiment the disclosure relates to methods to stimulate ilower bud formation its plants, in one embodiment, the one hod comprises applying one or more bacteria! strains or compositions thereof to a seed, I ban or plant part in an effeetsee amount to stnmdate f;ow¾r byd formation. ' la one mttew& mt, the iml i esiaois to methods: to it m the slrsangtt ttt vigor of flower buds plants. !a one embodiment; the tnethod c mpr ses applying one or more bacteria; strains or imposition.* there f w a seed, a plant, or plant part m an esleetive amount to increase the sPcngth or ig r of dower buds n plants.
In one embo iment, die disclosur relates to methods to increase flower bod Idacboo η· plains, in one embodiment, the method comprises applying one or ntore b eteriai cede■ or conutos ons thereof to a seed, a plant, or pfani part in m effective amount to Increase flower bud function,
L Marigolds
In one embodiment the s e , piam or plant par described p¾ Section F above is a uenseow. in n ther embodiment, bacteria! strains, and compositions thereof that promote flower bud formation, flower bud stimulation, increase in the woe or flower bods, ntereaee the strength or eiger of the flower bods, or increase flower bud function n a marigold are Baci!i s my li uif'cie . /¾cm0ocoi¾s poiy yxa, a id conibinaiions thereof
hi another embodiment bacteria! strains, and com pos ons thereof that promote dower bod formation. flower bud stimulation, increase in the number of ilowe-r ends, increase so the slae of flower bods, increase the srren¾l:b or vigor of the flower buds, or increase blower bud -function ia a marigold are selected boo; the group consisting of: BaciHus imyh!kpiifi-iciem 22CPL B ciii amyhilqifcic ΙΑΑΡΑ .Pwote i yiQtiqwfyckm BSiS, P nUnxiiius poiymyxa ΛΒΡ166, and c m inatio s thereof
In another embodiment compositions that promote slower bod forroadom flower bud stimulation, increase is the number of lower bads, i crease in the size of do buds, increase the strength or vigor of the flower i-o Is. or Increase flower bud function In a nmrigold comprise .\sc//Ao? oopa'oOi ipoe'/eii.s 22CPI- and Bacill s iopyfedayipyfeePetv 1 SAP 4.
in yet another etnbodlmcnt, compositions that promote flower bud formation, flower bad sbnasiafiort, increase in the mindset of flower bads, Increase In she size of dower bads, increase the strength or vigor of the slower bads, or increase flower bud funct n n a marigold c mprise a fiaciU a-ny Uq ffaciens 1 AF4 and i¾¾:g/0s.s oco A:d/p;p¾cieos BSiba
In yet another embodiment, compositions that promote dower hud formation, flower bed stimulation, increase in the numb«r of flower bads, increase ia the size of flower buds, increase the strength or vigor of the flow¾r buds, or increase flower bad function la a marigold comprise BcK-Him
MiqtMicSe 22CP\ and Btxmhis t oiiqi fyc BSIS.
Ϊ» yet aoother embodiment, compositions that promote flower bud formation, flower bud sihmbatbo, mersase in the neob¾w of bower buds, increase ia the s?ae of flower bad-,, mcrease the strongth or vigor of the flower buds or inc ease flower bod function in a marigold comprise Bacillus
Figure imgf000095_0001
< 5 AIM, and Ikteiti s a yioiiquifac BS 18. t*. O !m»nt frm ighd
In :'.;·,· embodiment, he disclosure relates to bacteria; strains:, c m ositi ns thereon and mebasds thai increase total plant fresh weight. In one emb dment, the total plant ftss weight in plants is increased by a percentap selected moo the group aonsisiing of fmm 1% to %% from a% to 3%, from 5% t ?%, from 7% to 9%, from 9% to i 1%, bora j i % to i 3%, from 13% to i 5%, from 15% to ! 7%. than 1 %¾ e> 1 from i 9% to 25%. arid greater than 25% s compared to an untreated control
la one emb dinient the disclosure relates to eh ds ro increase total plant fresh weight, In one embodiment, the method comprises ap l ing one or more bacterial strains or comp siUoB* thereof to a seed., a plant or lant part in an elRctive am unt to increase totai plans frmh wegh
1. Marigolds
in eobodmefio fre seed, plant or lain part described in Seeboa % above is a marigold. In another embodPoent, baolenal aiming, and compositions t areoldhar Incrase total plant fresh wei ht are Ik lhiS kiitipiif ck S: Baa fs !khsiilfb is; and combinations thereof.
In another embodiment, bacteria! strains, and compositions thereof thai increase total plant bosh weight in a marigold ate selected from the group ecawoainp of: Boaillxs u yloiiqnlfacie .?fr%. Bacillus a yioliq ifhc& v 22CFI %Pa;d«.v f yhliguifacie BS1 a. Ba H ^ amyie!iqui/ociem ABP27S, Bacillus oo%-:;;ba7oo BL2K sad combinations thereof.
E, Total Plant Dry Weight
In one en rood i m n t, the disclosure relates to bacterial strains, compositions thereof a ed'tods that increase total lant dry weight, in one embodiment, the total plant dry weight in plants is Increased by a percentage selected from the :oeap eomistlnp of fern 1% to 3%, .from 3% to 5¾, from 5% ·;.· 7%, b n: 7% to 9%, from 9% to n%;ifr¾t 11% to 13%>, from 13% to 15%, from 15% to 17^ fr m 17% so 19%%k>m 19% to 25%, and greater than 25% as compared to an untreated eontrog
In one etnbodlroent, the disclosure relates to methods to increase total plant dry wei h, la one eafrodimeng the method e: ·..·.·: ns-cs applying one or more bacteria! strains or compositions thereof to a seed, a plant or plant part in an effective amount to increase totai plant dry eigh.
I . Martgndtts
in one emhodsioen;, the seed, plan! or plant part described In Seorlon 1 i above Is a marigold, in another emoorbmeop bacterial strains, am! com ositions thereof that increase total plant dry. weight are B cillm amyioiiquifyciem.
In another embodiment, bacterial strains, arsd compositions thereof that increase total plant fesh weight in a marigold are selected from the group consisting of cslhis cirnylolU si ci-e - 22CPI .
Ba isl
Figure imgf000096_0001
15.ΛΡ4, Bacillus a ylviiaul c ii LSisAOL Bacillus c lcliqiyfi/iiens BS IS, and combinations thereof. In ¾ne* «r embodiment, compositions that increase total plant dry weight in a marigold c m os
Figure imgf000097_0001
22 ?l and B cilius a yhBqiif ckm 15AP4.
In yet another embodiment, compositions thai increase total plant dry weight in a marigold com rse Ba tfhiS amykBkyBfycie 22CP1 end Badii rnyhiiquiyickiis BS · >k
2, Tosoaoes
in one endssdnnent, the seed, lant or p ta par described In Section H abo e is a tomato, in another embodiment, bacteria! strains, and compositions thereof that mcrease total plan- dry wei s are illus amyioliq ifack . Bacihm ieaaadbrads. and combinations th eof
in another emliaaliruent, bacterial strains, and compositions theeof that increase total plant fresh weight in a tomato are selected Iron? the ro p consisting of: . acHhiS nykkiqkfitck B827, B&ci m
Figure imgf000097_0002
y ·. B hihis ft oikpBfa ien LSSAOi, ···.;·· ://·» am /q ifif kr B !S. i>Y¾.v;¾,* !k nmifo B BA842, dot a/sy /cOsoidbo?a?s BL21, mo combinations thereof.
L Seedling Gen«i»attoa
Germination is the growth of an embryonic plant contained within a seed, ii results in the formation of the seedling, 'The seed of a vascular plant Is a small package produced in a rait or cone after the anion of male and female sex cells. All fully developed seeds contain an embryo and. in most plant specie some store of food reserves, wrapp in a seed COST, dome plants pfodaee a ing numbers oi seeds that lack eaibryos; these are called empty seeds d aever germinate.
Seed germination depends on both internal and externa { conditions. The most important eaternai factors kelude temperaure, water, oxygen and sometimes light or darkness. Various plants require different variables lor sncecssf l seed gertniaation. Often this depends on the individual seed variety and is closely linked to the ecological conditions of a la t's natural habitat,
hi one e ibodii enk the diselostae relates to bacterial strains, compositions thereof, and methods dial promote seed germination in on emb imen the disciosme relates to bacteria! strains, compositions thereof and methods that increase the number of seeds that germinate. In yet anodrer emb dim nt, the disclosure relates to bacteria! strains, compositions thereof, and methods that increase the number of viable seeds.
In one embodiment the disclosure relates to bacterial strains, compositions thereof and methods that increase the st n th or vigor of seedlings. In one embodiment the disclosure relat s to bacterial strains, compositions thereof and methods that decrease the number of empty seeds.
In another embodiment the bacterial strains, compositions, and methods disclosed herein promote seed germination. In one embodiment seed germination is increased by a percentage selected from the group consisting of atom 1 % to 3%, from 3% to 5%, horn 5% to ?%, bom 7% to %, from 9% to 11%, from 1 % so 13 from ! 3% io I %¾ from 15% to \ ?¾, from I /% to 19%, f^m Ϊ 9% to 25%, and greater •han 25% as congaa-ed to a tsnireated control
In anotger embodiment, di bacterid ssra eis compositions, ,a;d echoes disclosed hereir; can increase the number of seeds that germinate, la one ensbodlrneng the n m er of seeds thai germinate is increased by a peosentage selected from die group consisting of fern 1 % to 3%, horn 3% to 5%, ik>m 5% to 7%, from 7% io %, isom 9% to Π ¾, from 1 1 % to 15%, kan ! 3% to \ 5%, from 1 % lo ! 7% than 17% to s 9%, .fr m 19% to 253% and gseatcf than 25% as cossipared to an lawssssted control
!n another embodiment., the bacteria! steins, compositions, and methods disclosed herein car: increase the number of viable seeds or seedlings. In one embodiment, the number of viable seeds or seedlings ;,- increased by a percentage selected from the group consisting of iront 1 % to 3%, than 3% to 5%, no: s 5% to 7%, m -s c 7% to 9%. trcsss 97s to i 1%, born ! ) % to I 3%, from I 3% to 1 5%. from i 7 to 17%, frona 1777 to \ 9¾. fr m ) 9% to 2>3¾, and greater than 25% a conspia ed m an untreated control .
In another emfeod ent, the bacteria! strains, compositions, and methods disclosed herein can incre se the sh-ength or vigor of seedlings, hi one embodiment; the strength or vigor of seedlings is increases:! by a percentage selected Irscv tbe group consisting of I rem ί % to 3%, from 37s to 5%, irons 5% lo 7%, from 7% to % Po: n v¾ 1 1 %, from 1 1 % to % % . irons ! 377 to 15¾, ifom j %s to I 7%, from 1 7% to I s>%; fe ars ! 9% to 25%, and greater than 25% as compared to an untreated conssol
In another embodiment, the bacteria! strains, compositions, sad methods disclosed herein can Increase decrease die number of empty seeds. In one embodiment, number of empt seeds is dees-eased by a pes-ceoiage selected from rhe group consisting of from i % to s%, from 77 >· > 5%. iron; 37s 7%, frocn 777 as %4 from 9% to 1 1 %, from I 1 % to 13%, from 13% to i 577, from 13% to 1 77s, from ! 7% to ! 977 from 1973 to 25%, aad greater than 257s as compared to an untreated control
In one embodiment, the disclosure relates to methods to promote seed germination. in one embodiment die method comprises applying one or more bacteria! strains or compositions thereof to s seed in an effective amount to rom te seed germination.
In otto embodiment, tbe d eSosnre relates to methods to increase the number of seeds thai germinate. In one embodimen the method comprises applying one or more bacteria! strains or compositions thereof to a seed n an effective amount io increase the mnnber of seeds that germinate.
In one embod lmesrg she disc losure relates to methods to ines-ease the number of viable seeds or seedling In one embodiment, the method comprises applying one or mors bacteria! strains or compositions thereof to a seed, a plant or plant pars in an effective amount to Increase the number of iab e seeds os seedHngs.
In one embodiment, rhe d isc losure relates so m th ds to increase the strength or vigor of seedlings, 1st one embodiment, the method comprises applying one or more bacterial strains or eemposiitens thereo i 8 a imi r plant past io a effective amount to Increase the strength or vigor of seedhng .
In one embodiment, the rfiselosure relates to m thods to decrease the .number of empty seeds, fa otic eoibodiment the method comprises applying one or m re bacterial strasns or com ositions thereof to a seed, a plant or plan! part n an effective araoynt to decrease the number of empty seeds.
VI, P OMOTI G GROWTH IN SOYBEAN
In one embodiment, the bacterial strains, com ositi ns, avid methods disclosed herein prosnoie growt of a soybean plant. In one embodiment, the bacterial strains, compositions, nd snethods d isclosed herein improve the performance a soybean plant, in one em hod invent, the bacserial strai , compo itions,, and methods disclosed herein Imp ove the yield a soybean plain. In another embodiment, the bacterial stra ns, compositions, and methods disclosed herein i ove vigor a soybe n giant.
!« another embodiment, the bacterial strains, compositions,, and methods disclosed herein improve- stand (the number of plants per unit of area) of a soybean plant.
In another embodiment, the bacterial strains, compositions, and methods disclosed herein improve the pes-fotmance of a soybean plant by a percentage selected item the groop consisting of irons 133 to 3%, from 3% to 5%, iron! 5% to 7%, fcom 1% to 9%, from 9% to i I 99 from i ί % to 1 %, from 13% to \ 5%, from \ 5% to 1 /%. from 1 7% to 1 %, item 19% to 25%, and greater than 25% as compared So an untreated control,
in another emhodisTsesrg the baeteriai strain , compositions, and methods disclosed herein improve the yield of a soybean plant by a pe centa e selected from the group consisting f from 1% so 3%, ipsa- 5% to 5%, from 5% to 7%, oo a ?% to 9%, frosts 9% to 1 1 %, item l l ¾ to 13%, from 1 % to 15%, from 1 5% to 17%, from 17% io \ 9¼, from 19% so 25%, atid greater thars 23% as com a t to an untreated eonp'of
Iti another embodiment the bacterial $mim, compositions, and methods disclosed herein improve the vigor of a soybean oaes by a percentage selected from tire esoap consisting of from i % to 3%, from 3% to 5%, from 5% to 733-, from ?% to 9%, from 9% sx< ί 139, from S 1% ιο 1339, frosts 1 3% io 1 ¾, from 13% to 17%, from I ?% to ! 9%, b-s 1939 to 25%, and sseaas- than 25% as compared to an untreated control.
In another embodiment, she bacterial strains, compositions, and methods disclosed herein improve the stand of a soybean plain by a percentage selected from the group consisting of front 1% to 3%, from 3% to 539, from 5% io 739, from 7% to 9¾t from 93 to 1 1 s, from 1 ) % to \ :39¾, irons J 3% to i 5%, item 1593 as ] 793, from 17% to 19%, fr m i 9% to 25%, and greases than 25% as compared to an untreated conPol. in one embodiment, the discl sure relates to methods o improve the performance of a soybean plant, in one embo iment the method comprises applying one or snore bacteria! strains or compositions thereof to a seed in an effective amosmt to improve the performance -of a soybean plant. In one embodiment, the disclosure relates to methods to hnprove e ield of a soybean p a t hi cam eosbodimen the method comprises applying one or more bacteria! strains or eomposhiorea thereof to ft seed, a plant, or plant part. In an effecti e amount to im r ve the yield of a so b an plant.
in one embodiment the disclos e relates to methods to improve the vigor of a soybean piano In one embodiment, the method c mp ises applying one or mo e bacterial strains or compositions thereof to a seed, a plan , or plant pan in so effective amount to Improve the vigor of a soybean plant.
hi rse embodiment the disclosure relates to methods to impr e the stand of a soybean plant, in one emb diment, the method comprises applying one or more bacterial strains or compositions thereof to a seed, a plant, or plant part in an effective amount to Improve the stand of a soybean plant.
in one embodiment, the bacterial strain and compositions thereof for improving the performance, yield, vigor, growth and stand of a soybean plant Is ac-iUus
Figure imgf000100_0001
22CF 1 .
¥11, PROMOTING OKO TH IN CORN
in one embod me t, the bacterial strains, compositions,, and methods disclosed herei promote growth of a com plant, In one embodiment, the bacteria! strains, compositions, an methods disclosed herein im ro e the performance a corn plant In one embodim nt, the bacterial strains, compositions, and methods disclosed herein improve dre yield a corn plans . In another embodiment, the bacterial strains, compositions, and methods disclosed herein improve vigor a corn plant.
In another embodiment, the bacterial strains, compositions, and methods disclosed herein improve statu! (the number of plants per nasi of area) of a com plant.
In another embodiment, the baeteri&l strains, compositions* and methods disclosed herein improve the perfor ance of ¾ cor plant by a percentage selected from the group consisting of from l ¾ to 3%, from 3% to 5%, Iron. 5%. to 7%, from 7¾ to 9%, from 9% to 1 1 %, from 1 1 % to \ 3%, from 1 % to 15%, from 15% to 17%, from 17% to 19%, from 19% to 2S¾, and greater than 25% compared to it entreated control
in another embodiment, the bacterial strains, compositions, and methods disclosed herein improve the yield of a cons plant by a percentage selected from rite group consisting of ft m 1 % to 3%, from 3% to S¾-y ;;. »;;; $% ·,.- 7%, from ?¾ to 9%. from 9% to 1 1 %; iron: 1 1 % vo 13%, from 13% to 13%, fern ! 5% to i 7%, from 17% to 19%, tv m 19%, to 25%, and greater than 25% as compared to an entreated control
In another embodiment, the bacterial strains, compositions, and methods disclosed herein improve the vigor of a corn plant by a percentage selected man the grotrp consisting of from i % to 3%, from 3% to 5%, from 5% to 7%;, from 7% to 9%, from 9% to 1 1 %, bom 1 1 % to 13%, from 13% to 15%, from 13% to i 7%s f o 17% to 1 %, from 1 % t 25%, and greater ihan 25% as compared to an untreated control.
In another embodiment, the bacterial strains, compositions, and methods disclosed herein improve the stand of a corn plant by a percentage selected from the group consisting of from 1% to 31%, from 3% to 5%, »« 5% to ?%/rr »rm o mjk3i59W !y n¾, inwn H % to 13%, ;h m l 33iH.o I S . fr io 15% to \ 7%, from 17% to 19%, from 19% to 25%, 3od greater than 25% as compared to an animated control. in one embodiment, rhe disclosure relate ½ mediods o ap rove I he peri rt anee of a com plant In one emb diment the meih d comprises applying one or more bacterial strains or compositions thereof i a reed, a plan?,, or plant part in an effective amoun to improve the performance of g corn plant
In om embodiment, the disclosure relates to methods to improve the yield of a com plane In one embodiment the method comprises app!ysag one or more baetenal strains or eontjsositions thereof to a s«i, s pfea , or plant st in an effective amount to dis rove the yield of a corn plant.
in one embodiment, iht- disclosure relates to methods to improve ihe vigor of a corn plant in one emb dime ;, t re method comprises applying one or mere bacte al strains or compositions thereof to a seed,, a plant, or plant part in an efteeUve amount to iarprove the vigor of a eon; plant
in one embodiment, the disclosure relates to matlsods to improve the stand of & corn piano in one embodiment the method comprises applying one or more bacterial strains or compositions thereof io a seed, a plant or pkm part in an effective aaioaal to improve the stand of a corn piano
In one embodiment, the baeieriai strams and compositions thereof for improving the performance, yield, vigor, growth an stand of a w eav plant are selected fern the group consisting o /ondi/io amy oi -Hfacw 22CP1 and B viH s intyhUqmf fcrx 15AP4.
VIII. .PROMOTING GROWTH IN WHEAT
in one cnibodimem. the bacterial strains, compositions, and methods disclosed herein promote growth of a wheat plant, i one embodinre!if the bacterial strains, compositions, and methods disclosed herein improve the performance a wheat plant. In one embodiment, the bacteria! strains, compositions, and methods disclosed herein improve the yield a wheat plant. In another embodiment the bacterial strains, compositions, and methods disclosed herein improve vigor a wheat plant.
In another embodiment the bsreteriai strains, compositions, and methods discl sed herein irnpKwe stand the um er of plants per nrat of area) of a wheat plant
In another embodiment t e bacterial strains, compositions, and methods disclosed herein Improve the perbo marwe of a wheat man;, by a percentage selected irons the gro p consisting of from 1 % to 3%v from 3% to 533, from S¼ to 7%, I ons 7% to 9%, h ore 9% io 1 1 %, from 1 I % to 13%, from 13% to 15%, from 15% to 1 ?%, from 17% to 19%„ from 19% to 25%, mo greater than 25% as o i!s ared to an normaied control.
in another em od m , the bacteria! strains, compositions, and methods disclosed herein improve the yield of a wheat piam by a pe entage selected fksro the group consisting o fk>m 1% to 3%, from 30 to .5%. horn 5% to 733, % so 73s to 9%. titan 9% to 1 1 %, foam 1 i' % to i 373, torn 1373 to 157a, from 1 S% to 1 ?%, from 173% to 1933, from 19% to 25%, and greater than 2S% as compared to an vnuv.wxl control. In anodser embodiment, the bacterial strains, compositions, and methods dhe sed Herein improve the vigor of g wheal plant by a pe centage selected from the group consisting of from 1 % to 3%» from 3% to 5%„ from 5¾ to 7%5 from 7% t 9%, hwo 9% to i I %, torn i 1 % to 13%, from 13% to 15%, fro 15% to 17%, bom ! 7% t \9%:. from 1 % to 25%, and greater Ite s 2S% as compared ro an untreated control
In mother emb diment the- bacterial strai s, compositions, and methods disclosed herein improve th stand of a wisest p!era b percentage selected from the group consisting of from I % to 3%, from 3% io S¾, b wv, 5% s ?%, from 7% to 9%, from 9% t I \%, from 1 1% to !3¾, bo* a 13% to I 5%, from 15% ro I 7%, from 1 % so 19%, moo I 9% io 25%, nd greater than 25% as compare so an uistreatod control. in am embodiment, die disclosnre relates to methods to improve the performance of a wheat plant, fo one embodiment, toe method comprises applying one or owe: bacterial strains or compositions thereof to a seed, a plant, or plans pari in mi effective amount io improve the performance of a wheat plant
In ne ab - Cowo , the disclosure - oCtc- s methods to Improve the yield of a wheat plant, in ne emhodknem, the method comprises applying <me or mote bacteria! strains or compositions thereof to a seed, a plant, or plant pan in an effective amount to improve the yield of a wheat plant
In one embodiment the disclosure relates to methods to improve the vigor of a wheat plant, in one embo ime t, th tnsi od comprises applying one or more bacterial st ains os compositions thereof to a seed, a plant, or plant part in an. effective s oont Pa im e the vigor of a wheat plant
In one embodiment, pse disclosure elates to methods to improves- the stand, of a wboai plant In one embo imen the method comprises applying one or more bacterial strains or compositions thereof to & seed, a plant, or la t part in an effective amount, to improve the stand of a whea plant.
in one -m l: wenwm:. the bacterial strains and compositions thereof for improving the performance, yield, vigor, growth and stand of a wheat, plant are -••fwwa from the grou consist ing of B dHus
i!g tfyckns 22CPI , LkiciUtts
Figure imgf000102_0001
BvcUiits amyi k u f i f f.SSAO 1. and eons b i a at k a ; s t.h ereo f
In one embodiment, the bacterial strains, compositions and tnethods disclosed herein may be used with a wheat cuhivar that is diploid, polyploid, tertrapiold, and hestaploid may bo used .
in another emb dimen , any eu!tivar of wheat may be used inefrtding but not limited to hard red spring wheat, hard white wheat, durum wheat, soft white Spring wheat, soft white winter wheat, hard red winter wheat, c mmon wheat, spieH wheat, emnier wheat, pasta wheat ami tarddum wheat. In another embodiment, die wheat is Triticnw rawCvwm,
In one embodiment hard red spring whe t includes but is not footed to uliseye, Cabernet, Cat Roto, Hank, Joaquin, Ceise. Lsrtat Lassik... aibee, Mika., PR 1404, Redwing, Summit 515, SY 334, Triple IV, Ultra, WB-Patrom WB-R ckland, Yecer Rrfjo, Accord, Aim, Ακ¾ Baker, Beth Hash its. Bonas, Borah, nm, Brooks, Back i ¾¾\ Butte 06, Cawdier, Challenger, Chick (';,,;-·· TAX. CoGsa, Companion, Copper, Ctryania, Dash 12. Eldoig Lna.no. Ex ress, Es ress , jefi½sors, G¾nero L8L Grand in, Helena 55 -k BoiiR, Irmsris T79. nia 66R, Jerome, Kern, Lea. Marshall McKay, Nomad, Northwest 10, Oslo, Paeon f ?C, Pegasus,. Pine 62, Poeo Bed., Powell Proban ?kk Pro-brand '·;.
Probrand 77. L Probrand 7 5, Pr brcd, Promts Queguay, Pronta Quintal Rich, RSt 1 SagiRaiio, Vwrk; Serra. Shasta, Solmo, SpUlrnan, Sprk .·. Sander. Stellar, Stoa. Success, Summt Sunstar 2, SunsLv King, Tadinnc Tammy. Tanori 7] . "Para 2y 0, Tempo. Tesia T?<>. Topic. US Winchester, Vance, Van al W4Ak Wamponr Wared, WB-Fuaion, Westbned 906PL Westbred 1 L Westbred «26, West bred 936, Wesibred Discovery, Westbred ambo, Yolo, and ZeLe,
n another emb dirivcnk hard while wheat includes bra is net Imbed to B!anca Puerto, Blanca Grande SIS, Bkmcs Roysle, Clear White, Patwui, Fatwin 15, 8~Cnsta!!o. WB-Pakona, WB-Peria, Alia Blanca, Blanca Grande, Delano., Golden Spike, 1D377S, Riasky E^oehsa, Lo! . Ma , Ot v Phoenix. Pima 77, Plata, Pnstine. Ramona 50, Skete Cerros 66, Vaiolet and Winsome
In et another embodiment, durum wheat includes hm is not limited to Crown, Desert inp. Desert King HP, Damking, Fortissimo, Hayasu, Kronos, Maestrale, Normanno, Ortta, Platinum, O-Max. S! 59, SaragoliS; Tango. Tipal Topper, Utopia, Volante, B-Mead. &tmore, Aldente. Ald a. Altar 8T A rata, Bittern, Btavadur., Candura, Cortes, Deluxe, Desert Titan, Dnre , Dnrfory Eddie, Oem tins 5003D, imperial Kola, Levante, Matt Mead, Mexicsli 75. Minos. Modoc, Mohawk, Nudura, Oc-ottiio, Prodnra. Reva, Ria, Septre, Sky, Tacna, Titan, Trump, Ward, Westbred ¾0w Westbred 881, Westbred 883, Westbred iOOPD, Westbred Laker, Westbred Lab,, and Yavsros -l
In another embodiment, soil white spring wheat indndes bat is not limited to Aipowa, A Hums. Babe. Div% JD, New Diflwin, Nick, T ifuWhit. Bianea, Bliss, Calorwa, Centennial Chalks. Dirkwin, Eden, Ed al!, Fiekler, Fieldwia. Jubilee, Louise, Owens. Pem wivvig Pomerelie, Sterling, Sunstar Promise, Super Dirkwlm Treasure, UI Cataki , Ui Pettit \hquk, Vanna, Wabnel Wadnel 94, Waluutz, Wa!iaday, Wawawai, Whitebird, and Z&k.
la still another embodiment, soft vyhke winter wheat includes but is not limited to AP Badger, AP Legacy, Bmrtdage 96,, Brunean, Csra, Goetee, Legion, Mary. Skbes, Stepbens, SY Ovation. Ttsbbs, WB- Jiaap! n, WB-52S, Xerpha, Yamhill Barbee, Basin, Bitterro'..«tt Bruebl, Casmn, Chukas-. Coda, Daws, Edwin, Elian, Faro, Pinch, Poote, Gene, Hill Si, Hi Iter, Hubbard, HyaC Hyslop,. Idaho 587. Kmor, Lambert, Lewjam, MacVicar, Madsen, Malcolm, Masand, MeDerrnid, hloro, rsgainsfs, O CF-lQi, ORCf ·· ) 02, O CF-U , Rod, Rohdo, Rn!o, Siruon. Saltne, Teajple, Pres. Tubbs 06, IBCP-Brundage. WBC2.1. and Wearherford, tn another esr ocHment, hard red arier wheat Inekales but is limbed to Andrews, Archer, Bstnm, Blscsoard, Bonnnviiki, Boundary, Decks De!orts, biniews Garland, Batten, Holy, Longaora Manning, Meridian, Pr omontory, V'ona. Wanser, Wiaridge,
In another eai odanei L common w eat (riexaplotd, free threshing), Triticum amt tm ssp mstivim includes bast Is sot Itrniied to Sonera, Wit W !korfn^ Cbsddam Bisne Do Mars, Indk-Jamma, Foisy.
in still an ther em bod la n, a ek wheat (feeasploid, not tree thrashing), frhi w n - nven; sap .vyc to uKdudes but is aot boused o Spanish Spelt Swiss Spelt.
la yet an the embodiment, Emmer Wheat (terapk>idy Tri c i rgi u sew. aacoceam includes bur is sot limited to Ethiopian Blue "Dago.
la another embodiment, pasta wheat iietraploid, fore threshing), Triric i rurgidim M durum i cludes bat is noi banted to Slue Board, Eturaardraq,
In yes another cmb dimesn, Turgidam Wheat {teirapioki fires threshing), Yiticnm mrgidnm ssp tnrgidurn includes but is aot limited to Akmotinka, Mapareha,
IX, INHIBITING PATHOGENIC BACTERIA
In another embodiment the disclosure relates to bacterial strains, compositions, and methods tor inhibiting bacterial pathogens of plains In case embod ment, d;e disclosure relates to a method for reducing bacterial damage to a seed comprising treating a seed with a bacterial strain or coasposmon disclosed harem.
In yet another embodiment, the disclosure relates to a method for reducing ba teri l dasrsage to a gioo: or plant pars comprising treating a seed with a bacteria! strata or composition disclosed herein. In yet another embodiment, the disclosure relates to a method for reducing bacterial damage to a plant or plant part comprising treating a plant or plant part with a bacteria! strata or composition discl sed herein, it) one embodiment treating the plant part comprises treating a part selected fwm the rou consistin of: leaves, sterns, branches, r ts, aerial tips, fkavers, buds, or eonrhirsaJion thereof.
In one embodiment she dtselosnre relates to a method for reducing bacterial damage to a plans or plant part comprising: (a) treating a seen with a bacterial strain or composition disclosed herein prior to planting, in another ernhedhnent, the utethod farther comprises: S b) reatin a plans part obgoned from the seed wuh a bacteria! strain or composition disclosed herein. The bacserial strain or composition nsep in tag (a) may bo she same or different than the bacterial strain or composition used in step (by
In yet another eusho iitjem, the disclosure relates to a method for reducing bacterial damage to a plant comprising (a) treating the soli surrounding a seed or plant with a bacterial strain or composition, in another embodiment, the asm- d bather comprises: fb) treating a plant part with a baefcrisi st ain or composition disclosed beroao The bacteria! strain or composition used HI step · m y be the same or dd'ibi -O than the bacterial strain or composition used in step ( ).
hi still another embodiment, ibo disclosure relates to a method r reducing bacteria! damage to a plant comprising (a) treshng a seed prior to planting oath a bacteria! strain or c m sti n disclosed he* em. io si-otber embodimem, the naebiod farther consprises: b? nasume the soil surrounding the seed or plant odth a boetenai strain or composition disclosed hemno in still another embodiment tbe method Anther comprises: ί c s iroataig a plant part of a plant produced irorn the seed with a bacteria! strain or composition disclosed herein. The bacterial srain or composition used in step ia ) may be the same or disferent than the bacterial srr in or c mpositon used n step (b). The bacterial stran or composition used in step (a) may be the ^ me or different than the bacteria; strain or composition used in step t c >. Tbe beoooai strain or coomosihon used in step b) may be the same or different than the bacterial strain or composition, used In s!en (e).
in yet anoth r ^ amdinieng the sod can be t eate with a bacterial strain or composition prior so treating a seed.
!a another aspect, the bacterial strains, compositions a d methods disclosed herein reduce damage caused by bacterial pathogens by about 10% 10 about 20%, about 10 % to about 30%.. about 1 % to about s0%, about 10% ;·· about '"·· ·.. about 20% to about 80%, about 30% to about 70%, :m-v.40% to about 60%.. or .shorn ago or more, about 10% or more, :d>ont 20% or more,, abour 30% r mor , about 40% or more, about 50% or more, about 60% or more, about 70% or more, about 80% or more, or about 90% or mote, about 5% or less, about 10% or less, about 20% or less, about % or a out 40% or less, about 50% or less, about 00% r less, about 70% or less, about 80% o less, or about 9 % or less oompa d to an untreated seed, plain, p!ani part, crop, inni or vegetable,
in yet another aspect, the bacteria! srmhas. compositions, and methods disclosed herein reduce cbarsage caused b bacteria! pa? he-gens by about ¾% about !0%, about 20%, about 3-0%. ab t 40%, about 50%, about 60% about 70%, about 80%. or about %.?% compared to an untreated seed, plant, plant ails crop, fruit, or vegetable.
in one em bod on en t, the disclosure relates to a method for inhibirmg gtoseth oi' acteria! plant pathogens comprising treating a seed with a bacteria! strain or composition disclosed herein, in yet another emb diment the disclosure relates io s method for inhibiting growth of bacteria! plant pathogens comprising treating a plain or plant part ih bacterial strain or composition: disclosed herein. In one embodimen treating the plant par! comprises treating a part selected fr m the group consisting of:
leaves, stems., roots, aerial tipo, (lowers, buds, or combinations thereof one embo imen the d C-tosure relates ½ a method for reducing haeierlal damage to is wd, a plant or pl nt part caa ed by ?'$gud<>;>mm s rmg comprising applying a bacterial strain or p s n disclosed herein to a seed, a plam, or plant part,
la 000 em dimen, the disclosure relates to a mehod tor inhabiting groo ah of .¾mia¾viv>i« . rijii y? comprising applying a bacterial strain or comp siton disclos d herein to a -see ,, piano or plant pari.
In era? embodiment the baemrhb t ms thai inhibit the grooah of F$ iidc t>?H:<s syHnga are B' iit seodoOgesMscmro and .Pa .nLbtx'B&s poiyrryxa. In one embodiment the B cBl :;
arityh/iq ifacie bacteria! strains thai inhibit the growth of Emina heroicola are selected from the group consisting of: Baei!kn a y llq iBhciens BS27, Baeuius cmyiollqidBac 5ΛΡ4. Bacillus
WKyk>!U{u.ijac ns 3AP-I.
Figure imgf000106_0001
S, Ba B! wyl B tBfl ns A P77B :-.n - eombinahons thereof.
In another emb dime t, the EmriiBiKilhis polymyxa bacterial eirairs that inhi is ho growth ■■[ F&emomonax sytitigae is Pamlbacilhts poiy yxci A BP! 66,
t. Etmtiii htrrbieofa
In 000 emb dimen, the di closure relates to a method for reducing bacosriai damage to a seed., a plant, or plant part from Erwino hiH- i al c m rising applying a bacterial strain or composition disclosed herein TO & seed,, 3 plmi or plant part.
in one embod -e , the disdosorc relates to a method for inhibiting ra ad of E^-Jnn her-bkoht eornprismg applyin a bacteria! strain or composition, disclosed herein to a seed, plant or plant pan.
In one embtxiageng the bacteria! strains dial inhibit the r m fErwin kerb tea ' are .Boebto an 7 lk tij k : and Pm wacBhis poly yxa In one embodbnem, the Β αίίίίκ anyloiUwtfbc xs ί'··.:·.οο !·Ρ strains i ai inhibit the growds oi · ·: mo hi-rb via are scieesed from i group consisting of B i is y lig lfacUws BB27, Bacillus ar BoliqtB/isaem BS20H4, .Bacillus c y Biq i/hciens 22CPI, Bacffiui nybBquif ciens 15APP B vU s ryioBqiiBBck SAP4. Ba lim ttyhik ifBekm LSSAOL Bacillus o/i?y /oignbc7i;os BSi$, Bacillus yloBqnBacie s ABP2~'3 and combinations thereof,
in another embrailmerrp the Pacnlb cJlk&yvoiynn'xa bacterial strain thai inhibits the groooh of Enaina herbkola is PiM'mh ciih® pofy yxa A BP lot?,
3. Ermmt chrymi'kem i
In one embo imen the disclosure relates to a method for r ducn bacterial damage to a seed, a plans or pbmi parr from Etwirui cfoysat <imctt -.eon -none applying a bacterial strain or composition disclosed: herein to a seed, a plant or pban part. In on*? embodiment, t e disclosure retrnes to & method for inhibiting growth of Aewano chrymtftem comprising applying a ba teria! strain or com os tion disclosed herein io a seed. plant, or lea part.
la one embodiment, the bacteria! strains that inhibit the growth af Erwitut cktymthenwi are /Aaomw (T yi ilqi t c s and /so '.w\:../.vb.- ;w; mmo in one emb d men , the ioett aa
iol mifbck roe a dd strains, that inhabit the growth of Bnvina c rysiuhe ai are selected from the groap consisting of: ikicHha- yioPq P ciens BS2084, B Pii un hliqmjaci s 22CPL BacHhiS yiotmd& ms f a. If- i¾nri/m? omvic Pigm/orw^y \ ppg. aciUifs emyioli fi i ns LSSAOf - com m/o fompa/o-oww MI S. B cilh^ myioiiqiiifisc ΛΒΡ278 and combinations thereof
is another embodiment the P mbaciihis poiymyxa bacterial strait; that inhibits the growth of £>-wmo :. h \ :-.;:i ;:!;;;■■ is Paenih cPi poiymyx ABPI 66.
X. INHIBITING FUNGAL PATBCX5E S
in another eubsodlmeni, the disclosure relates to bacterial strains, compositions, and methods for inhibiting fungal pathogens of plants, in one embodiment the disclosure relates to a met d for reducing fist! gal damage to seed, a plant, or plant part comprising treating a seed with a bacterial strait! or composition disclosed herein, in yet another embodiment the disclosure relates to a method lor reducing fungal damage to a plant or plant part comprising treating a plant part with a bacteria! strain or composition disclosed herein. In one- embodiment, treating the plant part comprises treating a part selected rom the groap consisting of: leaves, sterns, branches, roots, aerial tips, flowers, buds, or combinations thereof
la one embodiment, the disclosure relates to a method for redaesag furs gal damage to a seed, a plant or plant part comprising; ia) tra-atiog a seed with bacterial stram or composition disclosed herein prior to planting, la another embodiment,, the method farther comprises: (b) treating a plant pan obtained from the seed with a bacterial strain or composition disclosed herein. The bacterial strain or composition used In step (a) oar. be the same or different than the bacterial siraia or composition used In step (b).
In yet another embodiment, bee disclosare relates to a atethod for reducing fungal damage to a seed, piaab or p!am part comprising (a) ireabng the soil surrounding a seed or plant with a bacterial strata or composition. In another embodiment, the method further comprises: (h) treating a plsnt part sshh a bacteria! strain or conpgosition disclosed herein. The bacteria) strain or eoaipositioa used la step fa s may be the same or diilerent than the bacterial strain or composition a- ad In step (b).
in still another embodiment, the disclosure relates to a method for reducing itmga! damage to a seed, a plant or plant part comprising (a) treating a seed prior to planting with a bacterial strain or composition disclosed herein, in another embodiment the method further comprises: fb) nww -n: the soil surrounding a seed or plant with a bacterial strain or composition disclosed herein. In still aaother emhod rKmr, the medmd further co rises; ie l treating a piam pan of & p!««t produced fVons %·· seed wah a bacterial srram or com sit i n disclosed herein The baciem! strain or composition us d ;:tep (a) may bo he onne or di fferent dtan die bacterial strain or composition used n; step (b). The bacterial sarsm or compositio used u; stop fa ) may be the same or different than the bacterial strain or composition used in step fey The baotenaS strain or composition used in step ( b) may be the same or different than die bacterial strain or composition need io step fc).
in another aspect, the bacterial strains, oomposakae,.. and methods disclosed herein edoes damage caused by fungal pathogens by ah- ua 10% to about 20%, about 10% to about 30%, about 1 0% t- · aben 40%, ae on ! 0% so about $0 ., about 20% to about 80%, abo t 30% to about 70%, about 40% to about 60%.. or about 5% or mora, about 10% or more, about 20% . ^- more, about 30% or more, about 40% ot :0· -re about 30% or more, about 60% or more, about 70% or more, about bO% more, or %0tU 00% or more, about 5% or less, about ' 0% or less, about 20% or less, about 0%, or less, about 40% or less, about 50% or less, about 60% m le s, about 70% or less, a o a0% or less, <.» about 00% or less compared to an untreated seed, plant, plant part, crop, ruiL or v getable
In yot another aspect, Ore bacteria l strains, compositions, ami methods disclosed berem reduce damage caused by fungal pathogens by about 5%, about 10 ,, about 20%, about 30%, about 40%, about 50%, abciur 00% about 70%. , about §0%, o about 90% com ared to an untreated seed plant, pbeu part, c o ; is ait. or - <. ps aek
1 s t one embodiment, the d isclosure relates to a rriediod lor inhibiting growth of fungal plant pathogens comprising treating a seed with a bacterial xirain or composition disclosed herein, in yet another embodiment, the disclosure- relates to method for inhibiting growth of bacterial plant pathogens comprising treating a plan; or plant part with a bacteria! strain or composit on disclosed he in, in one embodiment, treating the plant part comprises treating a part selected from the group consisting of:
leaves, stems, roots, aerial Ops, flovsers. bods, or eombl nations thereof
.4, CfaOetatHehtm gmmimeoki
In one embodummg the disclosure relates to a method tor reducing O-nam damage to a pbast or pkmi parr caused by
Figure imgf000108_0001
a bacterial strain or composition disclosed herein to a seed, a plant, or plant pari.
in one embodiment., the disclosure relates to a method for inhibiting growth of Caitetotrickum gr n uc iu co prisOm apply ing a bacterial strain or composition disclosed herein to a seed, plant, or plant rt.
In one embodiment, the bacterial strains don inhibit the growth
Figure imgf000108_0002
are Baeilhis reu;t7id%« rpr%ius and aenidc /t ue m e sa .>o !n one erubodiiDersi. the Baciihts
y/oi;(p /ac!';>is ba te ia) I suaum that inhibit the growth < f Cor.hiioboluz eoedoouro are selected horn the group consisting of: Bu U a yf kppfy ie BS27. Badkux ci yhiiqi fyd s 2084, Badis s
Figure imgf000109_0001
Bad!i i ny Bqidftwic 12AP4: Ba &w; (cnykkkpalmdcrn 2 , Ba -Has myl fiqi.Pfiiei(:;n LSSAOh Badiim atnyi iqik/aciem ESI I, Ba i!i vs -mn oBquifxcc:>; Α.ΒΡ77Ρ and combinations thereof.
In another embodiment, the P rnb ciiii p ly yxa bacterial strain that inhibits die growth of C'ochlio hix rb num is Paerubacdkq poiymyxa ABP db.
B, C&c ihMus carbvnuffi
In one embodiment, the disclosure relates to a method for reducing bmgai damage to a eed, a plant or plant part caused by Cochiiobokis carbon un? comprising applying a bacteria! strain or composition disclosed herein o a seed, a plant, or p nt part.
In otic embodiment the disclosure relates to a method for inhibiting growth
Figure imgf000109_0002
carbonum comprising applying a bacerial strain or composition disclosed herein to a seed, plasm or plans part.
Irs one embodiment, the bacterial simms that inhibit, the growth of Cochbobalm a bon m are BacP'm ann4okq>qfii:ienx and Paenib ill pcAyc?yxc it: one emhodime-!Tg the
Figure imgf000109_0003
h- y iknrifacfcnt baciena! strains that :nhma the gcoyvtb of CochUobo/ carbonum arc selected a ..m the group consi ting of B dtim aniyhBpqfaciem Bc2 ' Bacillus amyiokquBoc ns 2084, BacBim amyk qdfycie<m22CPP Bacik myloliquifticie s Ϊ5ΑΡ4. Bacillus amylokqw/kck 3AP4> Baciilm
Figure imgf000109_0004
« Bii Hus amylokqit cie ABPB7S mid cornbnaiions thereof.
In another embodiment, t e
Figure imgf000109_0005
oiymx bacterial snain das; inhibits ihe growth of Cochiiobolm carbonum is Pmnibaciiiw poiymyxa ABF 166.
C. Fmarium grmttitt mm
f« one embodiment, the dsclosure relates to a method for reducing fimgal damage to a. seed, a plant or oi.na pari caused by Aw ri-o/o q.\s> c --c :.·α comprising applying a bacterial shton or com osition disclosed herein to a seed, a plant., or plans part.
in one embodiment, the disclosure relates to a method sbr inhibiting growth of Fusodua gramo-camm comprising applying a bacteria! strain or composition disclosed herein to a seed, plant, or plant part.
In rate en'ibodime , the bacterial strains that inhi it the growth of Fusartu grammean are
Bacillus iyioBqibjaeietis and PacnibaciHm poiymyxa. In one embodiment the Ba Hm
a yioUqu ck s bacterial strain that inhibit the growth of Fm'tfrmm g^a mearu are selected In so the grou consisting b Bacillus awyfaliipifacmx BS2?.. Bacili a yioBqiBfocicsw 208. Bacillus a»i4oliqidPacc- 22CPk Boclll iunykkiqukacic IP P4, Back; us aonlokq:o/ack:os 3AP4, Backks oeroo\nseio'san LSSA01, i i iikis oo .bgo:/ooieo; BS\n, Bcmii myb uifhc m J BP:'"- strd combinati n, thereof
; another embodbneoP the ;s -a.-o,a /•■•g an">o bacterial sirain ;0.n nhibits the gr t ,f Fmernm gm inearuw is .Pa t'nhaaUi pafy i ABPI66.
i, F sarit rB U e
i ne embodi ent, the disclosure relates to a method for reducing ftragal damage to a seed, plant or plant past from Fusaritm vmi & hdes comprising applying a bacteria! strain or mpasiriori disclosed erein to a s ed, a plant, or pl nt part,
la one e hodanent, the disclosure r-rin- ·...··· a a method for inhibiting growth of Fi M m v^rncoi odes om risin applying a bacterial strain or composition drsolosed herein to a s ed., plant or plant part.
b: one onbnabaont. die loammO strains ban inhibit the growth of Fmarhmi vers iPBoaes are di<; a.'.so sndoooopoo ¾- sad a^iH ^ poiy 'x . In one embodiment, the daOibvs
Figure imgf000110_0001
π;>·;ο¾/· verticolliodes -a-..- selected from the grou consisting of B ctiim ->aa dbs/ohS" ten;. -.Sd". f¾cd!/oa
Figure imgf000110_0002
20B4r Ba iilus a /oBquBluB&m UAPl ^BlBs iy Bi c m .bri'd $oo// amy iqw?ackn$ IMflSl. BiJclii am ioBqi f c^ s od H. nd connhinatknrs thereof,
la another embodbsem, the Pae?ubacili&s pok yxa hamrbd strain that iabibds the growth of /•av.esao? .voOs 0-sa s is P c hacis poiy x A Pibd. la e;mbodiaaonh the disclosure relates to a method for reducn ftaigai damage to a seed, a lant or plant part from Sckroimia sckmH m comprising applying a bacteria* smam or composition disclosed herein to a oa.-d. a plant or plant part
In one emhodbnent the discl sure relates t a method tor iohibidog growth otSckroHftia scisro omm comprising applying a bacteria' strain or composition disclosed herein to a seed, plant, or plant pars.
fa ons embodirnenr, the bacterial strains hat inhibit the growth of Sciero&ti s /tkt HM sre &«:da¾a fiwy ikiuijae/ ns nd ·.« v;Pa« BB-> /.ad: op SO. In one embodiment the dnvdo
Figure imgf000110_0003
bacterial strains that inhibit the growth of riv rwv-no ohv -— em are selected from the. grono consisbng of: /da sda v•.ao /'oao.s Λ ·;?.·> BB2 . Bacillus m;yl li iPfac-e d'-'-'g..Soobdas
Figure imgf000110_0004
ISAP4, Bacillus MP 4, ukB mnykdi uk cte bSSAOb ,% doo oaori ^pea ssew os;s BaeiHus lc qidfiia m ABP278, and combinations thereof in another eosbodknem, t e i'oc>xB<-x:::r; p- ii-sgxa bacterial strain thai: inhi t he growth oi S ierotinia sclerolkri Is .P erubaciii poiymyx ΛΒΡ 166.
F, C'Oi'Mehvius keie si pk
'in one embodiment, ihe disclosure relates to a nodsod for reducing fungal d ma e t a see , a plant, or plan! part Iron; Cochi boi s beierostrop m comprising applying a bacterial strain or composition disclosed herein to a eed, a plans, or plant part.
in one embodiment, die disclosure relates to a method for inhibiting growth of ' Coahiiobo'u s h-i rai-tripitfs comprising applying a bacterial str in or composition disclosed herei to a seed, plant, or plant parr,
la one mb dimen the bacterial s¾raio& that inhibit the rowt
Figure imgf000111_0001
keiem.nrop.us >;re
Figure imgf000111_0002
and Paent bacillus polymxxa. In one e bo imen the Ba iil
amysoikftiifbc fts Imgenal straao thai Inhibit the groveta of C chliohol s Ikxerosirophas are selectee from the group c nsisting, oh /bo όο.·,· amy ik{ui)d:l is /.A.;h /ha oho o.-m ovsgaaa aw-.2083. Bacillus amyivliqugB ieits 22CPJ: Bacillus a yloiiquifacimi icdP4, Bacillus anndaiiq ipjciens bo- ό Bacillus a ykdigsBfaclmx LSSAOL Bacillus amyhuiqvifyae BSU, Bacillus tny iig cims ABP2 ¾ arid combinations thereof.
in a other em odimen the P<muba?iihs ol iriy s bacterial strain thai in i is the growth of Cocidiobolas hete-'-js-r hiss is Paenibaciliiis poiymyx? ABPlbb.
G, lUpkidhi nydis
ha one embodiment, rise disclosure relaes a - a method for reducing fungal damage to a seed, a plant, or plant pari from Dipiodia m^ydis comprising applying a bacterial strain or composition disclosed herein to a seed, a plain, or plant part.
In one e bodbmng the disclosure relates so a ioediod ho mhibitffg gr--om of Dipkdia ittcp'dis c m risin applying a bacteria! strain or composition disclosed herein to a seed, plant r plant part. hi one embodiment, do- bacteria! strains that inhibit the growth of Dipiodia avdis arc oocoho
Figure imgf000111_0003
Pcs qsh illiis polymyx-a, In one eriih dhisene the Bacillu <Hm'ioliqsPiaciens bacterial strains thai inhibit the growth i Dipiodia aydis are selected from the group consisting op . acillus aimkiiqaifdeiem BS.I ". Back a-, amyioBqtqfaclens 2084, Bacdl amyiol kaca s 2 CPL Bacillus oro> /· AgaKc.-A-o $JP . Bacillus amykdiqtq/dcie 3AP4, Baciilm c^;yloliq iiac; s LSSAbh Baclllcs amciciiqia/dck s BB) §, Bacillus i-myk/kpild km .·.;/?/ Y*".v and combinations thereof
In another won. ohwoet. the Paenibacilhis paiym a bacterial sr in that inhibits, the growth of Diplodk; maydis is Paenib ci polymyxa ABP16b. hi o embodiment, the dscl sur relases io a method for reducing ftmgsi damage to a seed, a plan? or pbsm p«;t e-aavd by /';.>,·;·>/¾/¾ o f:<: <.'.;. ;.·;; cean sin p l g ·■ bacterial anna or composition d c!osed heein to a seed, a plant, or pb,m pari.
In one embodiment, the disclose relates tt> a method for mhibiho; growth oi£e-iero/b/oa; tt&' ic comprising applying a bacterial strain or composition disclose? herein to a se d, plant or pimi p .
!a ooo oafoodooeoo ihe baoierbb strai .* ihai irbohii the gro rh of ¾¾o¾>fo¾oa rare/cos^ are lki>:iii ofl e broAobeoA and Paenibneltius pofy yx . In one embodiment, the <iooi/?o- (. yiaHi i ackrtf bacterial strain*: tha inhibit the growth of Ex erohiinw turcic ae selected from the group consisting of: /.·;, ;pa.. cmyiohqulfih'wti-i BS27, Baci'i smy!ollquifa km 2084. BacH! s myl iiquifack ,'.' /',·' Bacill amykBiquB ck'-s 15ΛΓ4 BaciBm nmwHiqmfiX- n MP4: o'o; '/.be my i/qsB LSSAbh Ba lim KyhBkmx ns a /i /Wi kBqiJBuck BSPb . and eranbsnaiion-s thereof
in another embodiment, the Paetiih ciHus poiymyxci bacterial strain that inhibits the growth of Exs rahmm f icu is PaenibaciBus poiy yxa ABP166.
/, Fusarium rgi ifim
in one embodiment, toe dlsckwnre remuo, to a method tor doeing; fimgai damage ;o a seerg a plani or plant pari ir Fusarium virg-idiji-nn comprising applying a bacterial strain or m sition disclosed herein to a aeed, a plan- or piam part.
In one embodiment, the disclosure relates io a method lor in ibitin growth offmerfym v'rgirftfot'ffie comprising applying a bacterial strain or com ositi n disclosed herein to a seed, pi&eh or plant part.
in one embodiment the h&eierisi ¾itaii½ ihct inhibit ihe growth of rbaoarnwn
Figure imgf000112_0001
are Baci!his amyioiiipnfaci s and Pa&;ibi2cii!i& (rfym x8. In one embodiment, the B cii M
{ n rsquif cfctxi bacterial strains that inhibit the rowth of Fusarium virguii/brme e selected from ihe group coronating or
Figure imgf000112_0002
B&ciL ti sm ioikuifiickm b x ; Sfr nlus aw hi; i<ifiic ¾ί .w< 7 Factiiui
Figure imgf000112_0003
/emmma a ;o 0 B'-i B iii umboiiqulfhcie LSSAOi. Bac!li mykHiq fack B' :y Boctilm myioBqutf em ABP378, and eombmations thereof.
In another embodiment, the Pa k c his p !ymyxa bacteria! strain that inhibits the growth of F "us rmm virxiuifb- e is PaenibaciB poiymva ΛΒΡ I 66 In one embodiment the dlseiosnre relates- to a method Ibf redaeing bmg l damage to a seed, a plant, or phmt part from Pyfhk spierichns comprising applyn a bacterial strain or composition disclosed herein to a seed, a plant or plant part.
in one ernbodinterg. the disclosure relaes to a method ft;r inhibiting growth of Pyh m xpbtnda comprising applying a bacterial strain or composition disclosed herein to a seed, plant, or plant part. in one embodiment, die bacterial strains that iahihii the growth of Pyihw s/Pcnclam are Bacillus amyloP ifacicm: Fa ibacUkiS pofymyxa, and /teveav iich &!formis.. in one embodiment, the Bixi!hi £nwiolkpBfi<€ie?ii bacteria! strains that inhibit the growth f Pyi hm? spieruhns ate selected from the group consisting ot: Bacillus my!oiiqiiifciens S27, Bacillus a rvhliq ifiKicm tSSAOi. ?oc///«r
Figure imgf000113_0001
BSli, and combinations thereof'
hi an t er embodinmnt the Bacflim li hcnifyrm^ bacterial strain that inhibits that hdhbns the growth ofpytkium apicadom is . aiilws B:ke fhrmh' BA&42,
In smother embodiment, he PmmbaciHm pofymyxa bacterial strain that inhibits the growth of Pyihmtn splendam is B enlb cilhis pafy yxa ABPlbn,
K, fytltiam gmmimcffi
In one embodimen the disclosure relates to a method bar reducing fungal damage to a seed, a plant, or plant part f rotn PyPuum g minicol'a comprising applying a bacterial strain or computation disclosed herein io a seed, a plane, or pleat part.
It? one embodiment Par disclosure relates to a method for inhibiting growth. ofpythiwn graminicola comprising applying a bacteria! strain or composition isclosed herein to a seed, plant or plant pan.
In rate embodiment the bacteri l strain ihst inhibits the growth of Pj'tkium grwtiinscoki■ P nib cPliis p iymyxa. in anohe embodiment, the Pae baclllus pofymyxa bacterial strain that inhibits the growth of Pylhmw gmrmrsicoia is Paexibc&iH palymyx ABF166.
L ifythium ii otvci
In ·.:■:·,· embodiment the disclosure relates to a me d for redne g fungal damage to a seed , a p!aats or plant part front Pythmm. dismtocttm comprising applying a bacteria i strain or composition disclosed herein io a seed, a plant or plant part.
lit one embodiment, th disclosure relates to a method for inhibiting growth of F urium Pyihiu dissoioc n c mprisi g applying a bacterial strain or composition disclosed herein to a seed, plant or plant part.
in care embodiment the bacterial stems that inhibit the growth o pythium disssPocma are BaciHui amyioliqai/aciens and F Un^ll to pclyn xa. In om embodiment the Bacillus tm fo i<u(?ix'W?s bacterial strains that inhibit ihe growbi ΐ fy'thh d iac are selected from the groep consisting of .B dihis awyfost iiiitick-ns 22CP1. Sii iiii-a m hi piififcdns ! 5AP- a iihn a y < ui/acd LSSAbl, .ί¾οί;/οΐ o?m-fci ?{?<x:hms BSl 8, ami eonfoinabons thereof
l« another ensbodimerrg the fa tnhaci!l s pofymyxo bacterial strain that inhibits, the growth of /•o/;.ao dixsoioci i is
Figure imgf000114_0001
poiymyx ABPlots
X.t E 'ATOCi A'L EFFECTS
In another embo ment the disclosure relates i ba era! strains, compositions, and methods for i hibiting nematodes, i another embodiment the dscl sure relates to bacteria! strains, compositi ns, snd methods for killmg nematodes, fa one em o imen the ow.-b · oo .··?: ■·.·:' to a method ibr rednenig nematode damage to a seed,, plant or plant pan corrsprismg tresimg a ¾ed with a bacterial strain or com sition disclosed herein, in yet another eo bodiment. the disclosure relates io a method tor reducing nematode Passa e io a seed, plant or plant part eosygsrising treating a plant pari with a bacterial strain or composition, disclosed herein, mit embodimen, treating die pain past comprises treatin a par? selected from the group consisting of: leaves, sicms, branches, roots, aerial tips, flowers, buds, or combinations thereof.
In one embodiment, the nematode may be any ema de including but not homed to
Caenorhabdhis elegens oi2, Soybean Cyst Nematode, Sout ern Rooekooi Nematode. Colombia Root knot Nem isale.. and Renbbrm Nematode,
in one embodiment the dsclosu e! relates to a method tor reducing nematode damage to a seed, a plant or pi. an part com rsing: (a) treating a seed with a bacterial strain or composition disclosed herein prior so planting, In another embodiment, die method Peine; comprises: (bi treating a plant or piam part obtained from the sees! with a bacterial strait? or composinoa disclosed herein, The bacterial strain or composition used in step (a) may be the same or ditrercnt than the bacterial strain or comp siti n need in step (pi.
in yet another embodiment the disclosure relates io a method for nematode fungal damage to a seed, plant, or plant pari: con nsing (a) treating the soil snrrounding a plans with a bacterial strain or composition disclosed herein, In another embodiment, the ioeihod farther comprise : (b) treaimg a plant or pkir.it part with a bacteria! stram or composition disclosed herein. The bacteria! stoon or c- ear- ·. soon used '■< step (a) may be the sa e- or different than the bacterial strai or composition used in step (bp in si i another b dim t the dlsclosine relates to a method for reducing η···η:ϋ -A-, damage to a seed, plant or plant part comprising (a) tresbeg a seed prior to planting with a baeeenai strain or Composition disclosed herein, in another embodiment, the method further eoiviprises: b) ireahng the soil surrounding a plant with a bacterial amain or composition disclosed herein, to still another embodime t the method finther comprises: go) ireatiag a lant or plant part of a plant r duce from the seed wit a bacterial Sp ain or coro:poskion disclosed herein The- baeterkj strain or com osition used in step (a) may be di sam or different than die bacterial strain or composition used in step fb). The bacterial strain or c m osition used in ste (a) no;, be the same or different than -he bacterial strain or com esglon used in step (c). The bacterial strain or co posi ion used in step (b may be die same or different t n the bacterial strain or coni sition used in step l eg
In another aspect, the bacteria! strain.-;, compositions, met ods disclosed herein reduce damage caused by nematodes by about 10% n - about 20%, about 1 % >o about 30%,, about 10% to abotd 4-0%, atom 10% to about 90%, abou 20% to about 80%, about 50% to about 70%, about 40% to about 60%, ot about 5% or more, shout 10% or more, about 20% or more, abooi 30%.· or more, a OMi -40% or more, about 50% o snore, about 60% or more, about 70% or more, about S0% or more, or about 00% or more, about 5% or less, about 10% or less, about 20% or less, about 30% or less, about 40% or less, about 50% or less, about 00% or less, about %)% or less, about 80%, or less, or about 90% or less compared to an untreated seed, plant, plant part, crop, fruit, or vegetable.
In vol another aspect, d e bacterial trains, compositions, and methods disclosed herein reduce dama e caused by nematodes by about 5%. about 10%, about 20%, ab u 30%, about 40%, about 50%, about 60% about 70%·, about 80%, or about 90% compared to an umre;ded seed, piang plant part, crop, fruit or ve etabl ,
!n on embodiment, the disclosure mlates to a method for inhibiting growth ot nematodes comprising treating a seed with a bacterial ram or composition disclosed herein, in yef another embodiment the disclosure relates to a method for inhibiting growth of nematodes com risin treating a plant part with a bacterial strain or composition disclosed herea'a. In one embodiment, treating the plant part comprises Pl ting a an selected from the group consisting of: leaves, stems, roots, aerial dps, flowers, buds, or combinations thereof.
!:u one embodiment, the disclosure relates to a method or reducing nematode damage to a seed, a plant or plant part from Ca arhabdiits eieg m Ml comprisin applying a bacterial strain or composition disclosed herein to a seed, a plant or plant part
in one embodiment, Che disclosure relates to a method for inhibiting growth of Cmiio habdii eieg m N2 comprising applying a bacterial strain or coniposition disclosed herein to a seed, plant, or giant part.
In one e ibodisnesit, the bacteria! strains that inhibit Ihe growth οΐ d iorh bditis eiegam 2 are selected from the group consisting of Bacil!m
Figure imgf000115_0001
acillus liche tfbmtis arid Baeiihts so /Po in one embodiment, the &¾d//io/ a ylon ifbcie bacteria! strains that inhibit die gr w h ol' Co rhabdios eiegam N2 are selected from d e group consisting of; Baciiius mfoiiqi fickm 22%? 7, Bacillus wyfoHij ifaciem 2084, Sti us
Figure imgf000115_0002
22CF1, Bacillus a yhiiqtdfaciem 15AP4, Bacii'his ykilsqtiify i s LSSAO b Bacjii msy ni uifyviiins ifSI S, Ba iism mfyiM &uif ek AB 2 S, Bacillus xm io qinfocie 1013, ac ss
Figure imgf000116_0001
i;!)b combinations thereof hi one embodiment, the ¾,·.: o' o msfemgfeeeb bacterial strains that inhabit th wth of C mn- xbdtii-s ulegana N2 are selected from the group consisting of: BacsUtis ikhsnijbrwis A842 and B&cUhis mhernfbmm BL2 L
In another embo iment die anUi mhiiiis bacterial strain boo inhibits the grosvth ot
Figure imgf000116_0002
eoggnts N2 w Ba ili ub iHs BPS ,
ΧΠ. Ptoss asd Fl*nt Paris Ttereof
In ofifc ennKsdnrteni, wild plant speues and plant cmbvsts, or dwoe obtamed fey conventional biological breeding,, such s rossin or protoplast fusion, as id parts thereok cars be treated ebdi o or more bacierial strains,■·■ ·ίθ;ν···.··,ύ·;·'·. srsd methods disclosed herein, la bather embodiment, transgenic plants and piam cuklvars obtained by genetic engineering, (Genetically Modified Organisms), and plant parts thereof are u¾ated with one or mors bacterial stones, compositions and methods disclosed herein
Examples of plants incbtde bat are not leaned to cereals, wheat rice, cole crops, vegetables, fmfts, trees, liber crops, oh crops, tnber crops, cofsee, flowers, legume, cross, corn, corn, ' veet coop Hold coin., seed com. popcorn, oybea , potato, c tt n, tobacco, oilseed rape, peats ut, tobacco, grsoses, wheat, barley, rye, sorgbong flee, rapeseed, sugarbeet, syrrfknver. tomato, pepper,, bean, lettuce, potato, carrot, and i ii plartfs and fruit trees including bni not limited to apples,, pears, e tras fruits, peaches, nectarines, snavvberries, and gra e , as well as other plants or the monessayledonows and i s-tylsdonioss species.
In an aneaher embodiment, crops and plants capable of being used oath the discio -ed strains, om ositions and methods ineinde bat are not limited to pomme fruit,, stone fruit, girspevme, tea, almonds, nuts, eoftfce, tropical mm, soft fruit, ornamental plants, iawn, olives,, melons, b¾et. sngar beet, cereal oranges,, Clementines;, sarsnmas, lemons, grapefruits, en qusts, mandarines, apples, ptars, peaches, nserar es, cherries, apricots, tea, nntngoes, papayas, figs, pineapples, dates, bananas, dtaians, passion boa kakls. coconuts, cacao, coffee, avocados, lyehees, nmfaetgas, goavas, sngar caa . hazelnuts, treemus alnuts, pistachios, cashew nibs, brazil nuts, pecan nuts, batter rusts, chestnnts. hickory nuts, rnacadamna nuts, eanuts, blackcurrants, gooseberries, raspberries, b!ac!; berries, blueberries, strawberries, red bilberries, kiwis, cranberries, roses, carnations, getbtra. lilies, marguerites, chrgsanthemasns, tulips, daffodils, anemones, poppies, amarylHs, dahlias, aealcas. maives, gardemas, euphobias, bushes, oonrters, fig trees, rhododendron, s ru ? trees, fir trees, pine trees, yew trees, juniper trees, golf lawn, garden lawn, bell peppers, chillies, tomatoes, aubergines, cucumbers, pumpkins,, courgettes, broad beans, climbing and dwarf beans, peas, artichokes, lettuce, chicory, endives, carious types of cress, of rocker, larnbb lettuce, iceberg lettuce, leeks, spinach, Swiss ehard, ceieriae/celetg, beetroot, carrots, radish, horseradish, scofaooera, asparnggts, beet for human cotm mpttoiy palm hearts, bamboo shoots;, ftulfvermOfe bulb vegetables, omons, leeks, Florence leans!, garlic, Breessica vegetables such as cauiiflowess broeeob, kob!rabi. red cabbage, o i-iu- cabbage, cnrly kale. Savoy cabbage, Brussels sprouts, and Chinese cabbage, in one embodim nt, plants include but are not limited to Aster, Astiibe, Black-eyed Susans, Catmas, oarnadons, Conef!owers, Cosmos, Crocuses, Daffodils, Dahlias. Delphiniums, Gladiolus, Hyacinths, Impatiens, irises, Lilies, Marigolds, M rnin Glories, r½stnrtlum, Palsies, Peonies, Petunias, Phlox, Roses, Scduny Shasta Daisies, Sunflowers, Sweet Peas, Tulips. Veronica, Yarrow, and Zinnias.
Plants which can be tre ted: Include those of rhe varieties that are commercially available or in ase. Plant varieties are understood as meaning plants with, novel muos thai have been bred both by conventional breeding, by uta enesis or by recombinant foNA techniques. They m ty take the |¾rm of varieties, bjo ypes or genotypes. The transgenic plants or plant varieties ? planes or plant varieties obtained by means of genetic engineering) drat can be treated include all plants which, by means of the recombinant odif cat . Have received genetic material which c nf s particularly advantageous valuable traits to these plants.
Examples of such traits are better plant growth, increased tolerance to high or low iernperatafes, increased tolerance to drought or to water or soil salinity, increased flowering performance, facilitated harvest speedier maturation, higher yields, higher quality and/or higher wttritkmat value of the crop products, better siorability and/or proeesstbl!liy of the crop products. Other examples of such traits which are particularly emphasized are an improved defease of the plants against animal and microbial pests sack as insects, mites, ph aopaitmgeoie fungi, bacteria and/or viruses, and an increased tolerance of the plates to specific herbiesdai active compounds. Examples of transgenic plants include bat are not limited to crop plants such as cereals ( wheat, rice), snafee, soybean, potato, cotton, tobacco, oilseed rape and that pianos (with the fruits apples, pears, citrus fruits and grapes).
In another era bod intent, plants or plant c«itiv¾rs (obtained by lant biotechnology methods such as genetic etiyineering) that may be treated according to the strains, compositions and methods disclosed herein are herbicide-tolerant plants, mo plains m de tolerant to one or more given herbicides. Such plants can be obtained either by genetic transformation, or by selection of plants containing a tarnation iaipartmg such herbicide tolerance, Herhleldemesistam plants are for example giyplvosaie-toiamni plants, i.e. plants made lofeoant to he herbicide glyphosate or salts t ereof Plants can be made tolerant to giypbosale through different means, f or example, glyphosate-rolerant plants can e obtained by t nsforming the plant with a gene encoding the enzyme S-enolpyruvyisluieimate-a^hosphaie synthase (EPSPS),
Plants or giant caitlvars (obtained by plaid biotechnology methods such as g netic engineering) thai may also be treated according to the awestiaon are insect-resistant transgenic planes, f ee plants made resistanr. to amtek y certain tar et bisects. Such plants c bo obtained by genetic iranairssmn lon. or by selection of la ts contai ing a mutation imparting saeb insect resistance.
In another embodiment, plants; or l nt dd vara (obtained by plan; biotechaoiogy methods such as genetic engineering) that may■'■■ treated according to t e disclosure are tolerant ρ ·· aolonc stresses. Sued plants can be obtained by genetic transfomutlion. of by selection of plants containing a mutation imparting such stress resistance.
Naturally, hat has been said also applies giaat varieties wdheh will be developed, or a-i rkeUal the future and which haye these genetic traits or trans to be developed in the fu ure.
A, Applying feaefc?ria1 straps an ! coMposSikms to lmtts td pl&m ans
As used hereby applying a bacteria! strain or composition ro a seed, a plant, or plant past includes contacting the seed, plant, or plant pars directly arnl/or indmeetly whh the bacterial strain or com osition, hi one embodiment a bacterial strain or compos; dot; may be directly applied as a spray, a rinse, or a powder, of my combination thereof.
As used herein, a spray refers to a no a of liquid panicles thai con ain a bacteria! strain or composition of the present disclosure, in one embodimen , a sp a may be applied to a la or plant part whi le a plant or plant part is being gr wn, in another aspect, a spray may be applied to a plani o< plant part while a hnn or plant part is bein f rtilized, hi soother aspect, a spray may be applied ;o a plant or plant part while s plant or plant part is being harvested. In another aspect, a spray may be applied to a plant or plaid part alter a plant or plant pari has been harvested. In another aspect, a spray may bo applied to a plant or plant part while a plant or plant part is being processed, io another aspect, a spray may be applied to a plant or plant part while a plant or plant part is being packaged. In another aspect, a spray may be applied to a plant or plant p il while a plant or plan! part i$ being stored.
A spray may be applied directly to the plant or plant part using Items including, bur. not limited to, a spray can, a spray bottle, a spray am A spray can di enses a bsereriai strain or composition disclosed herein us ng a Hquid that turns into a. gas at room temperatme and pressure such «s
propssne/isohnane blends or PEEOhAM, or pressured gssses sueh as nitrous oxide or ordinary air.
As used herein, a spray botrfe is a bottle that can be used to squirt, mist or spray fluids. Spray bottles typicaily use a positive displacement pump that acts directly on the fluid. The pump draws Hquid tip a siphon tube from the bottom of the bottle, and the liquid Is forced out a nor le. Depending on the sprayer., the a ro b- may or may not be adjustable, so as to select between squirting a stream, a mist, or a spray.
As used herein, a zzk used ro apply a bacterial strain or com osition refers to s pr jec ing spo t from which a liquid is d scharged A nozzk may be plastic or rneah. As used herein, a spray gun refers lo a tool using compressed alt from a mzzh to spray a liquid m very small droplets in a controlled nei n. W en the bacterial strain or com osition is its e sprayed onto it field of pla t materials, e!eclrostabe tipnay is Ore preferred method.
In another embodiment, a bacterial strain or composition scl sed herein may he ap l ed directly io a plant or plant part as a rinse. As used herein, a rinse is a li uid containing a bacteria! strnfn or eomposrbon disclosed herein. Soeh a rinse may be poured over plant or plant part. A plant or plant an may also be immerse or sobmerged in (h rinse, then remo ed a id allowed to dry.
A rinse may be applied fe or more times to a plant or pkmt pan. A rinse comprising a bacterial strain or composition disclosed betem may be any concentrat ion, or speodkaby a concentration described herein. In one as ect a rinse may be applied to a plant or plant pari white a plant or plant part s being grown, in another aspect, a rime ma be applied o a plant or plant pari while a plant or ph i part is being fertilized, in another as ect, a Hose may be applied to a plant or plant pari while a lant or plant pan being harvested. In another aspect, a rinse may be applied to a plant or plant part after a lant or plant part has been harvested . In another aspect, a rinse may be applied to a plant or plant part wbbe a pianc or lan! art Is being processed. In another aspect, a rinse may oe applied a.1 a plant or plant pari wh ile a plans or plant pari b ing packaged, in another aspect, a nnse vnay be appl ied to a l nt or plans part while a plant or plant pari is being stored.
In another embodiment, a bacterial strain or composition may be applied to a p ttx or plant part atsd may cover 50% of the surface area of a plant material. In another embodiment,, a bacteria! strain or composition may be applied to a plant or plant pari and may cover a percentage of the snrface area of plant materia! selected from the or- -op eonsisbng of than 50% to shorn 95%, from 60% to about 95%, te en 30% to about 05%,, irons B0% to about 95%, and from 90 to a ut *%%,
In another as ect, ■■:>■■. i i:d strain or composition may cover from about 20% to a boa? 3 ,. iron; aboat 30% to about 40%, tr en abont 50% to about 50%, ft¾rn about 50% to sbout 60%, from
Figure imgf000119_0001
(.(} to about 70%. from abont 70% to about 8 %, from about 80% to about 90%, from abont 90% to about 95%·, troni about 0 to about %5%, t'rotn about 98% to about 90% or 1 00% of the snrface area of a plant or pi no part.
In another aspect, a bacterial strain or composition disclosed herein may be applied directly to a plant or plant part as a powder. As nsed herein, a powder is a dry or nearly dry bulk solid composed of a large number of very fine particles that may flow Freel when shaken or t ilted. A dry or nearl dr powder composition disclosed herein preferably contains a low pereenO e of water, such as, tor example, •a various aspects, less than 5%, less than 2 ,5%, or less than ! % by weight.
A powder may be contained in a jar or a canister and may be applied to a plant material by sprinkling or shaking. In another aspect, a powder may be applied to a lant or plant part by an apparatus attached to banning equipment such as a truck, a tractor, or a harvester, In one aspect, a powder may be applied to a plant or plant part while a p an? or plant part being grown, another aspect, a powder now bo appl ied to a lant or plant par; while a plans or plans, art is being fertilized. In another aspe t, a powder m».y be applied to a plant or plant pan while a lan or plant part is being harvested. In another aspect, a owder ma be applied to a plant or plant pars after a plant or plant parr has been harvested. In another aspect, a powder may be applied to a plant: or plant part while- a plant or lain part is being processed, in another aspect, a powder may applied to a plant or plana pan; wln!e a plant or plant part Is being packaged, In another aspect, a. pow'der may be applied to a plant or plant pan while a plant or plant part is being stored.
In another aspect, a composition ean be applied indirectly to the a plant or plant part. For example, a plant or plant part having a bacterial strain or composition already applied may be lunching a second plan;: or plant part so that a bacterial strain or composit on rabs off on a second plant or plant part. In a tin the r aspect, a bacteria! strain or composition may be applied using an applicator, i n various aspects,, an applicator may include, but Is nor limited t , a syringe, a sponge, a paper towd . or a cloth, or any combination thereof,
A contacting step may occur while a plant materia! is being grown, while a plant or plant part is being fertilized, white a plant or plant part is being harvested, after a plant or plant part has been harvested, while a plant or plant part is being processed, while a plant or plant pan is being packaged, or whi le a plant or plant pmi is being stored in ware ouse or on the shelf of a store.
in one aspect, a bacterial strain or eomposibon may be applied to a plant o plant pan., tor example, once a day, t ice a day, once every two days, once every three days, once every seven days, once every 14 days, once every month, once during each growing season, or one or more times while a plant or plant part is being grown,, wh ile a plant or plant part is being ferthimk while a plant or plant part is being harvested, after a plant or plant, past has been harvested, w hile a plant or plant part is being processed, w n!e a plant or plant part is being packaged, or while a plans or lant part is being stored. in another embodiment, a bacterial strain or composition, disclosed herein may be a colloidal dispersion . A colloidal dispersion is a type of chemical mixture where one sabst&nee is disperse evenly throughwn another. Particles of the d ispersed sobstanee arc only suspended in the m ixture, unbke a solution, where they are completely dissolved within. Th is ccurs because the particles in a colloidal dispersion are larger than In a solution - small enough ro be d ispersed evenly and maintain a homogenous appearance, but large enough to scatter Sight and not dissolve, Colloidal dispersions are an intermediate between homogeneous and heterogeneous mixtures end are sometimes classified as either
"homogeneous'' or "heierogeneoao' based upon then- appearance.
In anot her embodiment, ft bacterial strain or composition described herein is applied io dee foliar recion of a plant or crop from about 1 to about 100 days, about 2 to about :U? days, about Ϊ 0 to about 50 da ;, about 15 to abo t 40 cm. o about 20 to about SO days, about 30 to ou 50 days, about 40 to about SO days, about 10 to about 40 days, about 20 to about 40 days, or about 30 to about 40 days fs.n the aoOsl application to soil or seed.
In another emb diment... a bacterial strain or composition described herein is applied to the foliar region of a plant or crop from e%an | . about 5, about 10., about I 5. about 20, a boa- 25, about 30., bous 35, about 40. about 5. noes; 50. nboat 7 . or aboib 100 or more days alter 0;e Initial application O.s seal or seed.
la yet another aspect, a compound or co po>;iiion desoribed herein is applied to fhc lobar region of a plant or crop at least I , at least 5. at leas 10, at ast I S, at least 20, a feast 25, at least 30, at least 3 , at least 40, a- least 45, at least 50, at bast 75. or at least 100 days after the initial aoo a r tioa to sod i¬ saed, In another aspect, snore than cue of the above iohar application bases can be used with aa of the bacteria! strain, compositions- or arethods described herein. For example,, a bacterial srraln or composition desonbed herein can %· applied a- a first foliar application at alsota 10 days and a second fobar application a? about 20 Ο ,ρ ;, a first lobar applicatiou at abase 20 cays and a second fol ar application at about days, a first foliar application ai ab. -n; 30 days and a second foliar npphcauon at about 50 days, or a first lobar application at about 40 days and a second foliar ap lic tion at about 50 days.
In still another cmbodlmcab a bacterial strain or composition described herein is applied to tin; tobar region of a plant or crop at growth stage V2 - V3. In another aspect a bacteria] strain or cou!position described bes om is applied to the foliar region of a plant or crop at growth stage l - R2, In yes another aspect, a compound or omnposidon described herein is applied two or snore times to the foliar region of a plant or crop: Or-■ at growth stage V2 - V3 and second at grsns h stage Rl - R2.
In another aspect a bacterial strain or composition de c ibed herein is applied to the lobar region of a plans or crop at pinhea square tlnuog, In yet another aspect, a bacterial strain or composition described herein is applied two or
Figure imgf000121_0001
times to the Osiiar region of a plant or crop: at least one application at pinhca.d square and at least one more application after ab ut 10 to about 20 day s, after about 1 to about 30 days, after about 10 to about 50 days, after about 20 to about 50 days, or about 10 days, about 20 days, about 30 days, about 40 days, or about 50 days or snore.
!a ass aspect., the bacterial strai s;, compositions,, and methods described herein increase crop yield by about 10% to about 20%, than 10% t about 30%, about 10% to about 40%, about 10% lo about 90"% about 2055 ;o ab ut %)%, about 30% to about 70%, about 40% to about 60%, or about 5% or more, abont 10% or more, about 20% or snore, si on; 30% or more, about 40% or un ne. about 50%. or ur -i s . about 00% or more, abont 70% or more, about gy% or more, or about 0074 or more compared to an untreated seed , plant, plant part, crop, fruit or vegetable. m yet another aspect, t e bacterial ^irahaa oo p: " aoaw, and meOnxt describee herein increase crop yield by irons about' 5% to about, frorn about 10% to about 20%, dons about 20% to about 30%, from about 30% to about 40%, from about 40% io aboid 50%, from at sea 50% to about 00%, from about (•0% to about ?o%; bom aboat 70% in aboat 80%, tfom abosn 80% so aboia 00%.. from about % to abou 05%, from about 95% as-
Figure imgf000122_0001
100%, hvm about 100% aa aboat 1 5%. iron? abom 125% to acs 1 50%, from ab ip s 0% t abom 200%, and greaser than 200% as οοηφηί χΙ to an entreated seed., piano iam past, crop, fee a, or vegetable.
EML SEEDS
!.s ae embodiment the bacrerial strains, compositions, and methods disclose rein are suitable for use with a seed . In another eorbsodimenh dsn tsaeie hd oars eeursposhions, aaa methods discloaesl herein are snioible for use with a ,,,·, ! of one or srsore of a y of the plants i'eckeej in seebon Xfl
In -.00 another embodiment bacterial strains, compositions and method^ disclosed herein can be as- d to treat transgenic seed. This; refers to th seed of plants containing at k.-a- s one herero goy. --.. nc thai allows the expression of polypeptide or protein not naturally found in the pkmt The heterologous gene in transgenic seed can orsginaie. for example, b oa: microorganisms of the species Bacillus..
Rit obiu , P d m s, . -%'··-..cw. %c x %omw COrci aa ¾w Gfatm-s.1; e> (5/a> ½:¾or;.
la one emlxxd ent dse seed is teeaied in a state in which it is sufficientl stable so s'hat the treatment does not sacs; an dasvrage. hi general, treatment of the seed may take place at any poim in time between harvesting and sowmg. In one embodiment, the seed used is separated from she plans and reed fi om cobs, shells, stalks, coass. hairs or the flesh of she trusts. Thus, it vs possible to ^se. tor ex m le, seed which has beer; harvested, deaoed and dried. Alternatively, k is also possible to use seed which, after drysae has been treated, Oar example, with wares and thets drsed again.
In one ernb dmienk seed Is trearod ·.·· i;i; the bacterial strains, cos i ges io s arid methods disclosed h savin in such a way that ihe gen-amatkm of she seed is nor adversely affected, or that the resulting plant is not damaged.
i one -onbodb.x -u . Use bacterial sn asns, co p s tion and methods disclosed heroin may be useful on pri-iied and anprimed seeds. Pruning is a wares'-based ps'ocess known In the art thai is performed on seeds to lues esse umformity of germination and emergetioe from a or- -v. lag medium or soil , thus enhancing piam stand establishment. By incorporating the bacterial strains nasi compositions disclosed herein into the priming process, the bene ks of ' timum seed gessnsnation, o umu growth rid development, synehronixed bene to flower, uniform flowering, urdformsty m matssrity of she crop, improved yields and inaa - -ced quality of the harvested crop (fruit or odser plant parts} ma be obtained . The time span between the emerges;oe of the first and the last seevihrrgs may be dees-erased wish priming and fee use of the bacteria i strains and eompositiona disclosed herein as compared to priming aloae. Itroorporation of she bacterial xlrakax compositions and nasA A■ bwwo ad herein into the pruning oce s assy also increase tbe rate v i was; vwaxx so the plant stand st l ishes itself faster, ensui ing maximum eas oas of crop pet acre at harvest. Wide r nge ; in seed ling emergence decrease She am unt cd hareestable l nts per acre, which i an undesirable Abratlon tor the commercial grower,
Irs one embodiment, baefarial strains, and compositions isclosed herein can be ap l ed directly to the seed. For example, bacterial strains, eomposdioas and methods disclosed herein can be applied wit out additional emoposkaus and a u p. .;,·; avsaa been dilated.
in another em odiment. bacterial strains, and compositions disclosed herein are appl ied to the seed in the fonn of a suitable tbrmu!atUw. Suitable formulations and methods for the treatment of seed ¾¾ known to the pens -a skilled la the art red are described, for example. In die following docaurenbs: lib a ,2 /2,417 A. US 4,245,432 A. US 4,808,430 A. US a smo UP? A, U S 2AAy b AU 7S w wo
2002/080675 Ak WO 2002/02$ 186 A2.
I fx-: bacterial shsains, and compositions disclosed herein ear? be converted into custsaunry seed dressing formu lations, auch as solutions, emtbsiouss suspensions,, powders, roams, slurries or other costing materials tor seed, and lso UUV formulations. These ionnuUtmna are prepared In a known manner b mixing She bacteria! skuais, and οοηφοΑίΙοηϊ disclosed her in with customary additives, snob asp for example, customary extenders and also solvents or diluents, colorants, wetting agents, dlspersanto, emuisiikrs, de u -aao rs. preservatives., seco dary thickeners, adbesives, gibberellms and water as well.
In another embodiment, euinrbk colorants that may be present in the seed d sing fbnrsulations Include ad oolemma customary f sr sack purposes. Use may be made boih of pigments, of sparing solubility in water, and · f p s a im a are soluble in water. Examples that may be ment ioned include the colorams known under the designations Rhodaoune B, CJ. Pigment Red i 12. and C.I. S l ent lied k
In another embodiment, suitable wetting agents that may be present in the seed dressing formulations include all substances that promote wetting and are customary In the for uiatsoi! of active agrochenncai ^rsbstances. With preference it is possible to use alkvlnaphihslenewulpbonaies, such as di lsopmpy k or di isobutylna.phibalene--atdphonates.
in still another embodlinesH. suitable dispersants m)d - eaudsifiers that may be resent in the seed dressing formulations include all noniouie, anionic, and estlomc dispersants that are customary In the iom iabon of aetwe agrochcinioal substances, in one embodiment, nenkmic■■·· anionle dispersants or mixtures of nomonic or anionic dwperaants can be used. In one embod iment, noniomc dispersants include best are not limited to ethylene oxide-propy lerse oxide block ol mers, alkyiphenol polyglveol ethers, and trisp, rylphenol polyg! ol ethers, and their phosphates! or suiphated derivatives.
In another embod intent, amonie dispersanis include but are not limned to Hgnosulphonates, polyacryllc sabs., and
Figure imgf000123_0001
condensates. In st ll another embodiment deibamers thai may be present In the seed dressing formulations s be ased according to the invention include all foauaisdbbiiaia compoaods that are customary in the formulation of agroehenhcaily active a; px:ead- including but not limited to silicone deswes sero •oagnesluu; sicta-ate, silicone emoisloos. long- chain alcohols, tatty acids and their salts aad also oraano laorine compounds aaa mix ures the reof
n yet another enbiodimenP preservatives that nvas be presaal n da? s ed dressing !braadaPons include all compounds sn aaa be used or such purposes in agrocheudeal com ositions including hut not limited to dichlorophen and bengal alcohol hemifbrmab
In slid another eusbodiment secondary thickeners thai may be preseal In the seed dressing fonmdaiioas include ad compounds which can be ased for sack purposes la agroehemical compositions, oludmg but no? l an ded to ee!iulose derivatives, aer ho acid derivatives, poiySBXs;sharldes. saeh as xnnlhan gum or v eegnna modi fied clays, phyilosdieates, such as atlapnigiie aad bentonne, aad also bnelv d ivsded silicic ack
Suitable adheslves that may be present in the seed dressing formulations as be used according to the invention Include ail custouisay binders which can be used in seed dressings.
Polyvinylpyrrolidone, polyvinyl acetate, polyv inyl alcohol aad tylose may be mentioned as being preferred.
Suitable gibbereh lus thai: aaay be present la the seed dressing tornanaiioas inehale bat a e not l ea sed to da gibbvrel lins Ah A ?. (~ gibbereHic acicl g Aa aad A?; particular pre erenee is given a- asing guabercll lv acid. T he gshbereU ms are known (ef Kb egier "Chemie dcr Pllanaenscbaiss and Scbad- hngsbekampfongsoslPi-r Chemistry of C isp Froieoti< .« Agenw, and Pesticides ), Vol, 2, Spnnger Verlag, ! 9?0, pp. e0 b-4 ! 2).
In yet another cashed unenf seed dressing formulations may be ased directly or alter dilution with water beforehand to ts¾av seed of any of a e y wide variety of types. T he seed dressing iorrnaiaboos or then- oihne pssiparabens raay also be used to dress seed at rransgeoie plants. In this context, synergisbo effects may also arise a; arteractasn with the sudstanee>. Pasned by expression.
Suitable mixing eaa!pnseoi fbf treating seed with das seed dressing tbrnmiat nsva or the preparations prepared P om them by adding water includes ad mixing eqalpstaenr that can commonly be ased for dressing. The specific procedure adopted when dressing comprises introducing the seed ialo a rnixep adding the articular desired amount of see dressing fornuilation. either as it is or fo! sving dilution with water beforehand and carrying oai mix ing until the fonmnatior! |s ruutbnrdy di tributed oa the seed, Opboaahy, a drying operation follows.
In various es nbod anents, one or sn re bacteria! ssra , compositions or lomm latlons can be added to rhe s- od at a rate of about I x 10" to I a br enjorry fornbng ends (elu) pes- seed, including aboat ! x 10s cfu/seed. r a o t 1 x H? d ¾?sd, or about 1 x lib eta/seed, r about 1 x 10* cln/seed, inclnding about 1 o- 1 x 10 ollbper seed, about I x 10'' as 1 x 50V c I'd/per seed, abom I x 10*' o I x 10' etWper seed, aboui 1 x 10" s 1 a 10' carper seed and about ! x SO'' to 1 x 10* aid/ er saed.
In oaa enbxsihnena b.¾,.i :ad -ars;a · aad eomposbions eae be ionrebared as a liquid saed treatment. The s ed aeataiem comprises at least one bacaadai strabv The seeds are substantially uniibmdy coated with one or nana layers of bacterium, eeu-s conventional mehods of raking, spray mg or combination thereof. Appbe ux-n is dona using equipment that accurately, saf t , rid eipelenfiy applies seed tresPneat pfodticos to seeds. Such
Figure imgf000125_0001
-oa; various- o es of costing technology each as rotary . -sueo:..hum coaterx, fhablxed bed techalgnes spouted bads, rotary mlse> or a combinatio thereof.
la one eo'brsxii ent, the application ½ dons ia eaher a spinning "atomizer" di^-k or a spray xjde thai es enfy dlsnshuies the seed treatment onto the seed as a moves through the spray partera. la yet anmhar embodiment, tha reed is idea mixed or rambled lor an additional period of time as aehsrve additional tieaanent distribnboa aad drying. The seeds cars be primed or impriroed before coating with Ore inventive compositions to increase die uniformity of geradaatlon and ennvrgenee, ia aa alternative eofoodhraenr a dry powder composition ean be metered onto the moving seed.
lo add sa^he: emhodlivmng the seeds n¾n be coated via a eontaamus or batch a<.abng process, in a condnaons coating pix-eess. cs -nnnnons iloo eqolpaieai shnolameorsaly nasers bobs Use seed flow and the seed nvaument products. Λ slide gate, cone an orifice, · sari wheel, or weight device * bed or diverter) regelates seed flow. Once the seed flow rate through treating equpment is deierahaech rhe flow rate fif h seed treatanrni is calibrated to the seed flo rate in order to deliver the desired dose to the seed as it flosvs b-naqs; the seed treating eqaipnaaa Ackiidonalhy, a computer svsieai may monito die seed input as the eoadng asaehiae, thereby es^-ataix-ac a eoastant flow of the appropriate aaaiant of seed. la a batch coabng process, batch treatin equipment weighs out a prescribed araount of se«d aad places she seed into a closed treating chamber or bowl where the corresponding of seed treatment is then applied. The seed and seed treattneai are then mixed ro achieve a substantially mdfona coating oa each seed. This batch .is then daaaged org of he treaing chamber in preparation tor the treatment of die next batch. W th computer cormol g un s, this bvaeh process is automated eaabbng it to coiibnuousiy repeat the batch treaiaig process.
la either eoadng process the seed eoatlag machinery caa optionally be operated by a
programmable logic controller rhai allows various eqalpaseai to be started aad stopped without employee iatervenxien. The eongponenis of this system are commercially available through several sources each as Gastaisoft Equi men of Shakonee, Mam, A variety of additives ears be add«d v- thy seed treatments, Binders can be added nd include hose com osed preferably of an sdheslve polysaer d e can he nahwal or synthetic wlthoat phviotoxk ei vec on das seed to be coaled. A variety of colorants rates ensploved, ineiadiag organic chromophobes classified as tnrroso, aire; aso.. including roomerae, A-.aso. and polyaisa diplwnybnethaae.
trlarvbnethane. ;canihene, tnefhane, aerkbrre, rlria ole, rhbac e, Irrd&none, Inbophenoh mim, oxazine, amhraqaioono, and phiha es aasne. Gther additives that c n be added incltsde traee nutrients soeh as -saw of iron, niaaganese. isoroa, copper, cobalt, moiybdennne and zinc. A poiyoier or other dusi control agent eon be appl ied to retain b¾& rrentmenf on the seed sarfaee.
Giber convent ional i-eel h eatnreni: addit ives include, bat are a- a. bra bed so, coating agents, ettin agents, bnitenng agents, and polysaccharides. A t least one agncnian adly acceptable earner can. be added ro the seed treatment hwnkuation such as wnner, olids: or dry powders. The dry powders caa be derb eo from a variety of materials such as wood bssrka, calcinm c.o bonsw. gypstnn, eernfeetito, tale, hetnus, a; : :·■ :-c:. d chareoai, and various phosphorons compounds.
In one ennbodimeng the seed coating can comprise of at least one fiber., which Is ass organic or inorganic, natural or synthetic c- ·η·· ·.·η.οη with which the bacteria! si. re ar- and compositions theteof sne combined to facil itate its appl ication onto the seed. In -on. wnbodbneta. she fiber is an inert solid such as clays, natural or synthetic sil icates, silica, resins, wa s, solid leridl:rers ii'vr example a mornurn salts), natural sod minerals, such as kaolins, e!ays. Pale, base, quarto aocnpalgitw oognorihon ite. bentonite. or ibatoiogeeons earths, or synthetic mine als, saeh as sHrca, ahnnlna, or sil icates, in parbetbar aluminum or magnesium il ate .
In one ensbodimeng the bacterml sitcm. compositions, and mebiods disclosed herein ear; he used with geoetsoaiby m d fied seeds inc u ing bat not leaned to cotton, soybean, and corn (sweet, heal, eed or popcorn), in one embodiment, geneticall modi fed seeds sncirjde DELTA AND ΡΪΗΕ LAND® giyphosafe tolerant and insect tolerant cotton seeds he■■: ·. Monsanto; ETON EY ILL E N glyghosate tolerant and Insect tolerant cotton seeds from Bayer CropSe;ence; F!BERMAX® gh phosate tolerant and la- cw tole ant cotton seeds from Pba. es CropSeienee: eh gbss-we tolerante soybeans loam Stlne Seed Cosnnatiy, A SGhA OV A glyphosaic tolerant soybean seeds from Monsatuo; ΡΙΟΝΕΕΚΦ glypbosato tolerate and insect tolerant corn seeds from GaPong NORTH R.UP KI G™ glyphosate tolerant soybean eeds from Syugerbs; glyphosate tolerant and insect tolerant cos- a seeds from Burras Company; and Garst Com.pa.ns (AGE IGDGL'- '} giyphosate tolerant and insect tolerant com seeds worn Syngeota ,
The bacterial strains., compositions, and methods wil l be farther understood by reference to the toiiow ing psragraphs: 1 . A composition to inhibit a pliu.it pathogen comprising at feast one bacteria! s rain electe from Pre group consisting of Bacillus amylaliquifauiuns, a illu Ikheaifacms, Bacillus cabiHis, and
P bx iJus pcl nx hi an ei eA ive amount to inMbn plant pathogen.
2. A composition to improve plant psribri'nanee comprising at least one bacterial strain selected from the gr p consisting of Bacillus amyiaiiquifbek . Bacillus lichesufor s, Bacillus suhfuis: and Paeaibacillus polymcxa in r a effective am unt to nope.;-. ·:· plant perfor; nance.
3. A composition to impneve plant yield comprising at least one bacterial strain selected from the group consisting f Bacillus a ploliqiiifaciem. Bacillus Ikkeru/orms, Bacillus sub lils, and Buenas uuBui paly yxa in an effective amount to improve plant yield.
4. A com osit on to improve piant vigot comprising al least o bacteria! strain sele ted from the group consisting of Baalim auu'iulup.Bll:u:iensr Sacillas Bchcnmrms, Buskins sub Bs. and PacfkbacBlus pofymyxa in an effective amount to improve plant vigor.
5. A composition to increase phosphate availability to a plain comprising at least one bacterial strain selected from the group consisting of Bacillus mnypPuppfyc>e : Bacillus Ikhcaifartns, Bacillus subolss. and Pacnibacuius polymyxa in an effective amount to increase phosphate availabilitv,
6. A composition to increase t production of a plant hormone comprising at least one bacterial strain selected lo a n he group consisting of Ikf ill s amyloliquifaciens. Bacillus lichcmfbrrxs. Bacillus subkik, and Paetnbaciilm poly yxa m art effective amount to snorea.se roduct on of a plant hormone.
7. A composition to increase root formaton of a plant comprising at least one bacterial strain selected from the group consisting of Badlhts oo-v Apaaa a .<v il -. Biu.- BcBcuifor x. Bacill s subiilis, a Paerubacillvs palymyxa In an c iteei!ve amount to i ncrease root iormation.
8. A composition to increase shoot height in a plant comprising at least one bacterial soain selected from the grou consisting of Bacillus: a ylaliqnihckuy Bacillus ikhculBsrmx, Bacillus sub Bis, and Pc Bbacillu pidymyxa in an effective amount' to increase shoot height in a plant.
9. A composition us increase leaf length of a plant comprising at least one bacterial strain selected i ron - the group consisting of /toco/so a yloliquijackns. Bacillus Bche forms, Bacillus subiilis, and pcie acili ot rHixa In an effective amount to increase leaf length .
10. A composition to increase the flower bud formation in a plant comprising at least one bacterial stra in selected from the group consisting < B Ba Ao-. anpioBquifacicus, Bacillus Bckeniforms, Baculus subBli , an Bacnibisulllus polynwxa in an effective amount to increase flower bud formation.
1 1 . A composition to increase the total plant fresh weight piant comprising at least one bacterial strain · l ctee; from the group consisting of Bacillus arnyioiiquifackns. Bacillus Iklmn/or s. Bacillus subBBs, d Pacnibaclllus poiy yxa in an effective amount to increase total plant fresh we ight. ί 2- A com 3ii:k>n to•-••ococ iota! plant dry wei ht comprising st ieast one bacnanai strain sel cted from the groan consisting of iVoatyaa nyhii<i ckm. lio ss Ikke ifbrxU f< i!iu
Figure imgf000128_0001
d
FaenibaciSi s poiym xa in mi effecti ve amount to increase total plant dip weighP
O. A co pposits ii to increase seed geraunation com rising at least one bacteria) strain selected iron) i'---.- gronp consisting of ff Λ /-y» x v pulfacif- . >,.<. /.'to e Ρ".νί sww. Ba s!hs sabti 's. and
Faefiib isliss poly yxa in m ofi ctive amonnr to increase seed gemdnaiion.
14, The cornposition of paragraph i, wherein the plant pathogen is baeern
15 The composition of paragraph I , wheren the p!am pathogen is fungus.
16. The composition of paragraph 1. wherein the plant pathogen is a nematode.
I?. The composition of paragraph 2, wherein the m ro ed plant performance is selected from the group consisting oi increased root for alkm. increased root mass, increased root 1\η¾Ροη; increased shoot heighP increased shoot amotion, irrcreased flower bud p e ence, increased tlower bod tofmation, increased total plant wet waghP and increased food plan! dry weight.
18. The composition of any one of paragraphs 1-17, wherein the composition farther comprises ne or more a mhenwa!l active compounds,
19. The composition of any one of paragraphs 8, wherein the composition fmim- ·. ong.a : ·.."·· insecticide
20. The composition of any one of paragraphs f- 19, wherein the composgion farther cortiprises a fungicide,
2 S . The compo«nioo -d'-co one of paragraphs i -20. oh-vobn the o m nKm furher comprises a bactericide,
22, The composition of any · sue of paragraphs i I . wherem due composition further comprises nematieide.
23. The composition of any one of paragraphs J -22, where in the composition further com ri es a ssdener,
24. The composition of any one of paragraphs 1 -23. wherein the eoaiposition farther comprises a li o-chi Pa .! 1 i gosaceh arid-:? .
25, he conmosi!ioo of any one · o wayogdw 1-24, -.heron; die eoaav-^ -oon hnher comprises an isoflsvone,
25, The composition of any one of paragraphs 1-25, wherein the eomposhion iiirther comprises a ryanodine reeepnsr a. shoo a.
27, The composition of any one of paragraphs 1-26, v . he; cm the composition Rather comprises rynaxypyr. 2S. T y composition of any one of pm¾graphs 1-27, heoein the composition farther vomprises yaxypyr,
29. Th« composition of any one of aragra hs 1-28. wherein the composition further comprises a biocontrol agent, wherein the biocontrol agent is seleced from (he gr consisting of bacteria, fungi, yeasg protoeoas. vir se , erttomopathogenic nematodes, botanical s. tracts, proteins, secondary metabolites, and iono ulssna,
30. The compositions of any one of paragraphs Ϊ --29. wherein the strain is selected from the group consist ni of: Bacillus amvlollq facfe B527 (NRRL B~5(B5}. Baalim a yM i/asccs BS2083 (NRRL 8-5 1 ?■ . Ba ilfM amyloHqudackcs I5AP4 (ATCC ΡΐΛ··6:?07). Bacillus cmvloliqaiicclem 3AP4 (ATCC ΡΤΛ--6506). io, .¾; .;:ο 1¾;;ί;όο;ο^ LSSAiR (NRRL B~.¾(HiM). Bacillus
s !oaqiiiieckxs ΛΒΡ278 (NRRL .8-506.34 ); ίο Hie- amyhllqi fhci m 1013 (NRRL B-50S0Q), Bac.tiiw a^hlkp fdcie 9] S (NRRL B-50501), Bacil!us myt ilquifa ens 22CP1 i ATCC FTA-65 8), Bacillus amy liqmfoiiem BSI8 ("NRRi B-50b33), Btxii! s lichc for ls BA842 (NRRL B--50516), Bacillm llcl afoccvs BL.2! (NRRL B-S 134), Bacillus s bdlis 3BP5 (NRRL B-SOSiO). and
Faeaibaci!ias poly yxa ABP166 (NRRL B-S02I 0 and ombmabons there
31. The compositions of any ne of paragraphs 1-30, heem the composition comprises a combnation f'stnjins c-oraprisiog Bacillu amylollqiiiacicfis 22C?) and Bacillus G ykdiquifacis s Ϊ5ΑΡ4,
32. The ooro ostions of any one of paragraphs C31, wherein die composition comprises a combination of strains comprising Ba iii ; amyhHqitifiJciens 22CPi nd Ba illus αηη οίΙφ< ·:ΐ£ £ BSiff.
33. The compositions of any ne of Paragraphs B-32, wherein the cor«positton comprises a combination of strams comprising Bocli;: amyioHquij ciem BS i> and Bacillus m?yioliqiiilaciec 15ΛΡ4.
34. A method to inhibit plant pathogens comprising applying at least one bacterial strain selected from the go -op nsisting f Bacillus anivloliquilhcieus, Bacill iickefdlor s, .ComBs- o.N/C- and Pamlbacill s polymysa to a seed, a plant , lant part or soil in an effective amount to inhibit plant pathogens.
35. A method to improve plant performance comprising applying at least one bacteria! strain (selected from the group consisting of Bacillus a<nylollquifacierts, Bacillus I-i h nifbn , Bacillm scbPli, and PaeabaciB s polymvxa to a seed, a plant, pkmt pars, or sod in an effective amount to im r piant performance. 36. A method to Improve pfaat yield c m rising applying st feast one bacteria! strain selected from ?he group consisting of Saci!kns amb gx xcienSt B di! ehenifbr?n$. Bacillus ntbtdis, and o.;c-oo.;/.'0/.v.: pviy.wyw to a seed, a plant, plant part or sol! in an effe tive a;r5oi.ni to improve plant yield,
37. A method to unprovc plant vig r comprising applying &i least one bac enal strain selected iron; the group consisting or Bacillus artty iiipdf c& . Bacillus /VeAer/drow, Baalim sao/Pro nd
Paeaibacbiu poiy?t<yxa e a seed, a plant, plan; part, or soil in an etlAow arnount to improve plant vigor.
38. A method to increase phosphate av&i lability to a plant comprising applying at k one bacterial strain selected a - »ra the group consisung oi As A As
Figure imgf000130_0001
Bacillus AAnpAvsag A .. gA.- rtAoAo, aM i ttihaciii polymyx to a s ed, a plant, plant part, r soil in an effecdve amount to increase phosphate val!s!yiHty,
39. A method to increase the production oi plant hormones comprising applying at bast one bacterial strain selected from the group consisting of Bacillus amylailqudt dm, B cili PAenpems Bcx'Hhts iibahs, tmd facaibacillu:; po!y yxa io a seed, a plant, plant part, or soil in an efteotive amount io increase production of plant hormones.
40. A method to increase root fonruuioo of a plant comprising applying at least one bacterial strain selected iforn the group consisting of A a Ao- miyl liqidfhci , Bacillus lichcnifbrmx, Bacillus zubtiiis, ?d /¾ dvmy ii io a seed, a plane plans part, or sod in an effective amount to increase root ibnnauon,
1. A method to increase shoot height In a plant composing ap lyrng at least one bacterial strain selected from the group consisting of Bacillus myl lmiijacle s, Bacillus lich lo!ai-Λ. Bacdi s¾MAA and Bacibbacillus polymyxa to a seed, a plant, plant part, or soil In an effective amount to increase shoot height.
42. A method to increase leaf slae of a plant comprising applying at least one bacterial strain selected (s om the group consisting of Bacillus npd updiaci m, Baubles iichcrdfbrms. Bacillus snobby and Paeaibaciilm ροίνηρχά to a seed, a plant, e!am part, or soil in en effective amount to Increase leaf tsv
43. A method to Increase ilower bod formation io a plant comprising applying at least one bacterial straits selected Irons tin- group consisting of Bacillus amyloliaiucicic , BacUhts licbcu or s, Baciiiw oA/oev an Paeubacib t■ polym xa so a seed, a plant, plant part or soil in an effective amount to increase flower bed tbrntation.
44. A method to increase the total plant fresh weight plant comprising applying at least one bacteria! strain selected from the group consisting -.4.A.vo/e- amyioHquifbciens, Bacillus tkheru rms. Bacillus si bifS: and fbtmibiubl polymyxa to a seed, a plant, pl nt part, or soil in an effective amount to increase: total plant fresh weight. 45. A met d t«> increase total plant dry weight omprising a plyin m least otc bacterial srain seected fr m the group eoosAuna of Bacichis -ww / s-oAeAw; Αοο ;/;.* iichmifonm, Bacillus SMPO'AA ana1 faeinb ciil poiymyxa to a seed, a plant, plant pari, or sod in an effective a ount to increase total plant dry weight.
3D. A method to increase seed germination comprising applying si leasi on bacterial strain selected from the group consisting of ifooaasw i idiqni/hcd?; , illus Behetisf rm*. Bacillus saboAy, o«A Famibacul pcly yxa io a seed, a plant, plant part, or soil in an effective amount to increase seed ges-rnm&tioH.
4*?. The method of para ra h 34, wh««i» the plant pathogen la bacteria.
4 A The .method of paragraph . therein die plant pathogen is ftmgus.
49. The method of paragraph 34, wherein the plant pathogen is s nematode.
50, The method of paragraph 35, wherein the improved plan? erformanc is selecte from the group consisting ofinereased toot tbroiabon, increased root mass, increased roo; function, increased shoot ight, mcreased shoo? i'uncooa, increased fl wer bad presence, increased dower bad formation, increased total plant wet weight, and increased total plant dry weight.
5 ! . The- method of my one of paragraphs 3440. wherein the strain is selected front the group consistin of: Bxcii!w amyloli dfycicns BS27 (NRRL B-AOia), a asV
Figure imgf000131_0001
BS2i)S4 (NRRL BAOOPA Bacillus a^loBqmfyciem i 5AP4 (ATCC PT3 -o507}. Bad/his au^loliquldcle 3AF4 f ATCC PTA-6506), Becitf s omyioBamaciem LSSAOi ( R.R.L B-50104). Aoa/A.-.
a yloli i ciens ABPd'S (NRRL 8-50634), Bacillus a yloli uifacie 1013 (NRRL B-50509),
Bacillus anryfoifquifadms9\% (NRKL B-5050S). Bac i y Hq fa 22CP1 (ATCC PTAA508A SaciU s awyfrh vifbdrm BSi 8 (NER! tASOAwP Bacillus Ikb donnis ΒΛ842 (NRRL B-A SiffA Bacillus Bchefiirbrma BL21 (NRRL. B-50134), Badll subiilh 3BPS tM L B AOS 10), and
A cfbAocoAs p fymyxa AMP i 66 (NRRL B-S0211) ana combinations thereof
52. The method of any one of paragraphs 34-51» wherein the method oomprises applying a combination of strains compricrng Bacdlu a vioiiq ifhcW 22CP1 and Bacillus a/nyloliqiiif ie
15AP4.
53- The method of any one of paragraphs 34-52, wherein the nseihod comprises applying a com ination of strains eomprisirrg Bacillus a y il^ ifyi ns 22CPI and Bacillus aff oliqvifack
BSIA
54. The meth d of any one of paragraphs 34 -53. wheein the method comprises applying a combination of ^tr uw comprising AaoAw a yiaiiqwfaciens BS 1 S and Baciiias arrtyl ikuifyd ns 15AP4. 55. The method of any one of para a hs 34-54, wherein the method further comprises fl ing on or more agrochemicai y active compounds.
56. The rooihod of any one of paragraphs 34-55, hored? the method farther comprises applying an hweeticidw
57. The method of my of paragraphs 3-1-56, wh rein the method sVir or om rises apply ig a fwigicide,
58. The method of arty om. of paragraphs: 34-57, wherein the method further comprises applying a bactericide,
59. 'i'hs method of any one of paragraphs 34-58, wherein die method further comprises a pl ing a nematicide.
60. The method of any one of paragraphs 34-59, wherein the method ha her comprises applying a • ateaor
61. The method < T my one of rag aphs 34-60, wherein the method further comprises applying a hpo-chitooHgosaccharlde.
d2. The met o of any one of paragraphs 34-61 , wherein the method farther comprises applying mi iSoflavone,
63, The method of aw one of paragraphs 34-67, wherein d e mwnod further comprises applying a ryarsogine reoepwr nw>d» ka.
64, The method of asp ne of paragraphs 34-63 , wherein die method further c m rises applying rynaxypyr.
65, T e method of an one of paragraphs 34-64, ν« herein the method arthe comprises appl ng eya¾myr.
66. The method oi any one of paragraphs 34-65., wherein the method further comprises applying a hiocomrol agong wherein the bioeontro! agent is tected from the grxatp consisting bacteria, fungi, yeast, proio oas, viruses, ento opati ogenio nematodes, bommcai extracts, proteins, secondary metabolites, and innoouiatua.
67. A product comprising: a composition of any one of paragraphs 1 -33 and »t least one genetically modified seed, plant or plant part
68. A product comprising: a composition of any one of paragraphs 1 -33 and at least one seed, la or plant pan,
do. An isolated Bactt ts strain for improving plant pet idrnmnee, plant vigor, or plant yield, wherein the //www..; strain comprises the Boh A gene, and pvodnees lndok-3-aeetie acid.
70. An Isolated Bacillus strain for improving plant performance, plant vigor, or plant yield, wherein the ;7.a.3iw strain comprises the trpAB gene, and produces indole-3-aeetle acid. 71 , An isolated Bacillus tran for improving plant perfomanse. plant or or plant yield therein the Bac 'iw strain comprises i t pboP one, and the ability to solubilfoe phosphate.
72, An isolated Bacillus strain for Improving plant performance, plant vigor, or plant yield wherein the Bacillus stran comprises the phoP, BdhA, and trpAB genes,
73, Art isolated ί·-■■,:-;:·<! strain fo improving plant performance, plant vigor, or plant yield whes in the BacUl s strain produces tndolefS-acetic acid and has the ability to -.oh-bolsc phosphate.
The bacterial strains, compositions, and methods will be further understood by reference to the iodev. iop H>-a isaroov examples. The following Examples are provided for llhi t a o purposes only. The Examples ae Included herein solely to aid in a more complete understanding of the presendy described invention. The E amples do not linns the scope of the invention described or claimed herein in any fashion,
EXAMPLE 1
Si im with the aMiiiy (o st ahilize phosphate, in the soil
Figure imgf000133_0001
MET HOPS
Baciihti strains were n ed vitro ior their ability to sohfoilize calciant-bound phosphate. Calcium Pi-Phosphate agar (500 rnl drfo!k-d w ter, 7.5 g granulated agar, 0.25 g yeast exlract 5 g glucose, 2,5 g t>; Poof .0,25 g (NlipySCA, 0.1 g KCl 0,05 g MgSCfo 0.0001 g fos p.0.0001 g foefoo. sterilized at 12 Γ€ lor ! S minutes ) plates wans? prepared by pipetting 20 mi tempered a ar io 100 mm x 100 mm x 15 nun square disposable perri plates. Oa oho tain^ were grown at 32T in 0,5 ml Akitrierh broth in a 6~¾¾H deep well block, shaking at 130 tpm for 24 hoars. After the Badiiw strains had grown for 24 hours, 2 μί of culture was spotted onto the surihce of the solidified Calcium trhPhosphaie agar plates. Plates w etc incubated at A',: of agar side down, 0.·· 48 hours. Zones of eleanng svere aseasured ..a millimeters from the edge of the colony to the edge of the com-
Zones of clearing w¾e present tot the followhgg strains; /fo.s/0- li -h-i/or is BA842, Ba i!i Hc eniformL* BL2), Bacillus amyhliq ijckns BS27, if;, do- m lerfiquifiiti ? BS2084, foe Cfw os;A pfp?Ai.. /; so ] SA-P , Bacillm my liq lfoci n.-; 3A-P4, BcieiBw & yio;iqu{facie LSSAOf /;····· for-- • s/ov.Pnp.-.v, .·..».·; ΛΒΡ2/&. fos vhw av >hBqwthcit>w o;g. ;md θο· ·.<¾.·. Mikii 3B-P5, in millimeters from the edge of die colony to the edge of the zon .
Figure imgf000134_0001
Strains that contain the phoP gene
MATERIALS AND METHODS
A polymerase chain reaction (PGR) was rwn to amplify th« plant growth promoting gene pho?f W present. Eaeh reaetlon contained: 5 μΐ iOX PCR buffer, l.Std SOm MgCh I μ! lOnvM, dAS'TPs. OA μ! 2ΰρ / |if fePA i5¾-TGCTGCCGAAAGTGGACGGAA- 3'), OA yi 20plvi/ ul p oV-R {$'■■ ATCATCCGCTCCGAGCTCCAGT- 3*), 0.3 pi SU fnvitrogeft Platinum® Toq DMA polymerase.39.2 pi sterile ddH-O, and 2 μ! template gDNA for a total reaction volum of 50 μ.Ι. The PCR reaction was run on the Applied Biosysteins 2A20 themtocy er using the foll in program; initial denatoaiion 94°C for S minufes, 30 cycles of: denatumhon 94AC for I ruin, annealing 57.5AC for ! ran, ejosgg&r n 72°C for ! win, and a final elongation step of 72AC for 5 minutes.
After completing the PCR reaction, samples wer prepared for electrophoresis through a 1 % agarose gel (l,5g Bio-Rad Molecular Biology Certified agarose and !SOra! IX TEE buffer). Each well as loaded with 4 μΐ PCR product mixed with Sgl Orange O loading dye {I5g sucrose, 0.1 ?5g Orange G, and SOmi dd'H20). The gel was e1ectr«phore¾ed for S3 minutes at 85 volts in I TBE buffer then stained it; 0.173 Ethi mm bromide for 30 minutes before the gel image as captured with the Syngene GeneSnap software program (Cambridge, UK).
RESULTS
The expected size for the hoP gene product is 134 base pairs. Strains FZB42 (positive control). Baciiim mnybU idfaciem L8S AO 1 :. Baciiim amyhiic if ic 22C I , Baciiim im kB ti ckns 15Λ·-Ρ4: Baciiim amyioiiqu cietis 3A-P4, Buciiim ainy/oUqiiifaciem BS2084, Baciiim myiolkraiuciem BS27, Bacilhi<; mnyiciiquiiacie BS18. and Ba&iim anmoHquifhci s ABP278 were found to soma hi tb yAAT gene (F G. I ), ThtsphoP gene is responsible for positively regelating transcription of the phosphate egidoo thai s« n-,:o'-.'..:g: for aikahn phosphatase production, which is n oived ?a the ensyrnatk; fsvAsA-- of phosphate,
Straf harboring gmes ( & i&Md wiih pi i growth ka regulation MATERIALS AND METHODS
A polymerase chain reacti n PFCR3 was run to determine f the plant growth promoting gene hdhA was present in the tested bacteria! strain. Each reaction contained: 5 μ! I OX PCR buffer, I Aid 50mM MgCb I id ! mfvl, dNTP , OA μ| 2 pM pi /AC A; ($!-RCCGAlT1'rTGi.TrACACA G-3,)I 0.5 μΙ 2 pM pi Ao¾A-R ί $CTO g7GY7 . GGWCsAAAG--i ), 0,3 pISU Invh ogen PI;Ah».nrrA 7aq DNA poivmepase, 30,2 pi sterile ddFEO, and 2 μ! template gDNA for a total reaction volume of 50 nl. The PCR reaction was ran on the- Applied Biosystenw 2720 ihemxreyckr using the following pogram; init ial denaturation 94°C for 5 mirnnes, 30 eveies of: ck-naiuraiion 94°€ for 1 rain, annealing 20.5°C for 1 mm- elongation 72A3ior I mtn, and a final elongation step of 72 AC for rfonotes.
After completing the OCR reaction, samples were piepated for electrophore¾.is through a I % agarose gel (1 ,5g Bio-Rad Molecular Biology Certified agarose and 150ml I X TBE barter). Each well was loaded with 4 pi PCR product waxed with t¾ l Orange G loading doe GSg sucrose.0,17Sg Orange G, and 50ml dd'H20), The gel was eieeuophoiesed for 85 inutes a? S5 volts m i TBE buffer then stained in 0.i¾ Ethi unn broodde for JO minutes before the gel image was captured with the S ngene GeneSnap eAc.ee program (Cambridge. UK),.
RESULTS
The expected si e for the /CPA gene product is 336 base pairs, Strains I AAA (poAtive control's Bacilt mdidsquihcdns LSSAOC Badkua umvkdk pfyck 22CP 1 , Baciiim yl liqi cie I 5Λ-Ρ4, BdcUlits myioliqikfacie 5A2P4, >Aw amyid!qidfaciem BS20S4, Madi ntykdk dj ci ns oS2 ' 2¾w//ow yloiiqdfkc m BS! 8, and Ba iii - am- iiquijdc ---g A.BP27K were toond to contain■]■<■■: A/PA gene FIG.2- The bd A gene is associated with the production -A . , :- Aoo-nds ¾ volatile compound associated with plant auxin (growth hormone?) production and regulation,
Sireins mih the aMiiiy i& produce thtplenf h rmo , ditk~3~meik mid (MA) MATERIALS A D METHOOS
Ba k his strains were tested vkro lor now abihiy to produce IndoleA- Acetic acid (IAA) n; dee presence of tryptophan. Bacillus strains were grown at 32" C in 0,5 ml Nutrient broth (MB) in a 96-weii deep wed block, shaking at 130 $ rn for 24 hours. After 24 hours, 2pl of cultu e was transferred to a 96-wel! deep w il hiock coiHartiing 0.5 mi KB with the ;··.!·ΐ;ϋ·.··; of 2 rng/mi Lsiryptopha and grown at 32VCX shaking at 1 0 rpm for 48 hoars,
A 1000 ppm 1AA standard ¾a¾s prepared by adding 30 mg IAA (Sigma) to 5 ad EtOH, vortexed to dissolve, then brought to a final volume of 30 ml with the addition of 2S ml stmte HA), The !AA. standard w s serial diluted in B plus L-Tiypt p an∞u) tested ;*t 1 00.500, 50, 125,623,31,25, 15,625, and 7.8: 24 ppm.
The 48 hour cUlm growth w s pelleted by centrifuga on at 5000 rpm for 20 mimrie* and i 00 μ I of the supernatant was added to 50 pi Salkowski reagent (14 mi sterile HA), i 4 rrd 70% Perehlos ie acid, 0.500 ml 0.5M FeCl in a 96- ei! clear assay plate. In addition, i 00 μ 1 of NB plus L-Tryptophan and 100 pi of the IAA serial dilations were added to 3 pi SalkaswskiA reagent. The assay plate was incubated at room temperature for one hoar for a color change to occur, and then read■ a a Genios plate reader ffecan, aonedorf, Switzerland) with an absorbanec of 492 t¾m.
The absorbance of the negative control wed (NB plus LAfryptophari) was subtracted from ad values, A standard curve was created by plotting the !AA standard serial dilutions on an x-y scatter raph with absorbance along the y-aoch and ppm along the x-axis. A linear trend line w s added to determine the it. value and the slope was used to determine the ppm based on the absorbance of the tested Bacillus strains.
RESULTS
The ΙΛΛ standard cur e had an to value of 0.99 > 9, I A was produced by the following i¾¾:cAi;.s! strains: B ci!ius iic e fir u BL21, Bacillus amyhiiq ifyciem BS20S4, B J a e yiQiii fyci i$SAii Socuhii myioik ^ci s 1013, and Bacillus kiiis 3B-P5 (FIG.3). IAA stimulates cell elongation and division, which Induces root and shoot elongation. 7Abie 2 surnmarAes the parts per million of IAA produced by the MmiUm strains.
Table 2, Parts per million (ppm IA A. produced by Bacillus strains.
Figure imgf000136_0001
Stmins thai contain (he s mi genes irpAB
MATERIALS A D ETHODS
A polymerase c ain reaction ( 'C ) was run to amplify the plant go h promoting ne oy '-!r if present, in c-iU s strains. Each reaction contained: 5 μί I X ICR buffer, I.5ul 50mM MgC!2. ! ul l m.M dNTIG, OA μ! 2()pM pi i?pA ~F($*- AGCIGAATTCT!TCACCTTACCA 3 A, OA μ! 20pM/ μΙ PysAB-E ;5■ A Pi ATGGCTCA ATI A'GC ACC- 3'.), OA pi 50 fesvjtrogen Platinum* Sw D'NA polymerase, 39,2 pi sferue dd!GG, and 2 μ? tem late gDNA t r a total ta ti n volume of 50 μ|. The PGR reaction was run on tire Apphed Biosysteros 2730 iher.0K>cyck-T usng die following program: initial denaturatkm $4°C or 5 minutes... 30 ovdesoi: desatarauoa 94AG En- 1 m annealing 52.4AC for i msy ekmgitthsn 72Ά7 tot 1 roln, andafmal elongation step of 72 °C EM- 5 minutes.
After completing the PGR reaction, sarrrples were prepared lor electrophore i through a i % agarose gel i ! Ag Bio-Rad Mokeular Biology Certified agarose and 150nd iX T'BE bidier). Each well vow loaded with 4 μ| PCR product, mixed wn 8pi Grange G loading dye ( 15g sucrose, 0 i 75g Orange G, and 50ml ddH20). Tiro gel was electrophoresed for S3 minutes at 85 olts in IX T'BE buffer then Gamed in 0.1% Ethidiutn bromide or 30 minutes before ie gel image was captured with die Syngene GensSnap software program (Cambridge.. UKg
EES II LI'S
The expeeted w/e tor the PgAB gene roduc is 2,069 A: w pairs, Strains FZB42 op-Ate e ewer- Bad/ha a yhuqiiif a m LSSAO ! ( Bacillus (m>yiol Mimc 33501 , Bacillus amy iiqui -kM 15 A-P4, B difas iykyiqisj cie 3A-P4, Ba i m a ykll ifack BS20S4, Bacillus amykliquifack BS2A B df/us myioiiq lfa i iv BSGE and Bacillus
Figure imgf000137_0001
A BIG "A wens GwG to contain the rgoAB gene (FIG, 4), The AB gene encodes tiyptophan synthases an enz e necessary for symhe A of tiA pf pharp which A a precursor for tndole-3-AceOe acid (!AA). As discussed above, l.\A is a known plant growth - prof o otir.g hormone .
Strains m'fk the abUlty to produce comf mah with siib cisri i ejje
MATERIALS A D MPTHOSSS
Bacillus mkol jii c ns strains BS27. BS20A4, 22G-PA hSA-Pd, sA-ps, LSSAO E BSIA ABP2 AG 1013, and St! 8, and Paenibadihi poly ym strain ABP466 were grown in 30 ml glass test qdses containing ? 0 ml Tryptfc Soy Brot ("Ϊ SB f he stmm were grown at 32T2 shaking at 1 0 rpm for 24 hours Overnight culture was then inoculated at a E 100 dilution ink? a 250 ml Erlenrneyer flash containing 30 mi TSB and grown 32 "C, sh,ikir>g t 130 rpm for 24 hours, The entire cornersis of the flasks were eenififuged at 5,000 s ro for 20 ruinates. The ¾ip«roatam was then filter sterilised with a OAurn tliser, P Gnl -associated pathogenic haci na, A-.; oAeowo^ s/> aroc Eni-mia erbicofa, 07.·' P.- .so:.; oAwsosds -wos- grown in a mttriem-nch inedia for 24 hours.
An in ibiion roth assay was set up using a 96- el) microliter plate in which treatment w ls and two control webs were set up in duplicate for each pathogen tested. Tosaiment welB contained I SO μί T SB, 20 pi fllterwteriilxed sgoernatant, nd 2 pi overnight atho enic bacteria culure. Control wed contai ed 180 ϊ 1 si 20 pi sank peptone broth, and 2 μ 1 overnight pathogenic bacteria cul ure. Negative control webs were set up for both the treatments and control which contained eit er 180 pi TSB and 20 μΐ Bherwieniizecj supernatant, or ISO μΐ TSB and 20 pi sterile peptone broth, respectively.
T he 6-weiI plates were covered whh a plate seal '-ad incubated at 3'"'C ibr 24 hours, t he optcal density i O.D, for each well was detemtined using the XFtouH plate reader at 5 5nm absorbance. For each pathogen, duplicate wells were averaged for each treatment and control wells and the O.D. values for the egativ ontrol wells w s subtracted before calculating, the percent inhibition.
RESULTS
S he following strains demonstrated the ability to produce compounds with antibacterial effects against common pbint-ass ciated pathogenic bacteria in νίιτο
Badil Qy fahqmfw -ns BS27, BS20S4, 22 CP A laApso 3AP4; LSSAOI , BSI A ABP2-A; and
Figure imgf000138_0001
polymyx ABP! fed.
T he er ent inhibition of the pathogenic baciena Is shown m Table 3. One Psisec/omorim syringac strain, eight Envrnia herbkaiu strains, and three Erwinia ehrys itkeni strains were tested. Bacillus
Figure imgf000138_0002
ain md four of ihe 2 hcrbkda strains. Bad Hits a?»yidi u(fyckns BS2084 inhibited ail eight £ h rhicda strains and all three A h ysanx i strains. Bacillus r^la!iq ciem 22CP1 inhibited five of the £ herbmka strains and two of the £ oft/psorpdea?; strains. Badi hi* a yldiqdfackn 15AP4 inbnb cd the :" syringae strain, sis: of he B. kerbkda strains and two of ihe A, hryv nid l strains. Bacillus a yhlkrifackn^. AP4 inhi ited the A. y rbpgoo strain,, seven of the £ rikc la strains and ail three oi: the £. chry ihemi strains. Bacillus amyioliquk ck LSSAOI inhibited the P. syring e strain, ail eight of ihe £ erbico!a strains, and all tbwe of the £ chn>s nih?:-!-k stra . Badii c ryhiu foviens By i g inhibied the P. syrifig e strain, four of the ft', 2. A- .·.2.· strains and one of the A eeao¾omA?enA strains, / Α· ·;.·:.> ne.a Css/oAs a as A.BF22S inhibited the. P. syrdgae strain, seven of the £. As 2/, da strains and ¾j( three of the £.
c rsatHkeni; strains. Pact BhadB poiyttma ABP 166 inhibited the A. syringa strain, all eighr of the £ hcrbicala strains, and ail three of the A , soc-owAcw strains.
Figure imgf000139_0001
Strains with anfiftutgal effects egaimt comtnoft platft-asse ated p geftk maid
MATKRIALS ΛΝ0 METHODS
Common plan moid:; ws-re btaned from I θ·· weP- · ;..··,·! fo mhibkiort %:-0ng: C /ia'rotric nm vramm!coi , F ariiitn gra;n;;>ca>'um, Seiiiroisnin sc;eroiiorum; F ariv verBcBioidei,
Figure imgf000139_0002
arbo m, Fn7arkan ο; ·;%%;ο:?ο. Co hhobol s he -mfroph'-is, Exer hllum teri in?. υψ&άία mm?dis. Molds were struck to purity onto Potato Dextrose Agar (PDA ), then tfansisrred to a 30 mi glass test tube coma ring 10 mi Potato Dextrose Broth (FOB). The molds were grown at 25'C, shaking at 130 rpm for 7 days The Bacillus iny iiquefociem strains BS27, BS208 .22C-PL 17 Λ· i%.7% 03. LSSAOi, BSI8, ABP2 8. i 013, d 0 ! 8, B ciilm iichx fyrmis strains 8AS 2 and BL2 i , Bacillus subBiis strain 3B-P5 and Biicnib cUim poiy.nrsxa sirain ABPI66 wee grown to purity by streai- og trooen culture onto Tryptic Soy Agar (TSA) plate The ISA is !·.:··; were growa ai .7: C tor I. : h u s and osd promptly lor the assay. After ? days of growth, the mold on Pure wa rem ed from the incub tor and vortesed ns break the ball forma k>s>. Using sterile cotton swab, the mold ¾s struck ont ihe surface of a PDA plate rhree times, ot nioy rise plate 60" be een s» wkinw to obtain e en iooonlailon. Six dn'ferent BacUias drains w re ested on each swabbed plate by picking an is lated colony fBaciiha with■:·. sterl toothpick asd stabbing the agar surface. Each Bac lvs strain was tested ts triplicate. Plates were incubated at room ternperstnre for 120 hours; then zones of inhibition in she mold lawn wore measured and sceorded in millmeters of clearing distance from SwAAe; colony edge to mold edge.
Statistical nalysis was accomplished osn? GLM procedure of SPSS (version 1? 0: SPSS Inc.. Chicago, 11,). Mean separation was determined using Tukey Honestly Significant Di fferattce (HSD) test. Tor all analysis performed, significance was declared at P A 0,05,
RESULTS
These strains have s n tne ability to inhibit die growth of common plant pathogenic molds in vitro:
Bacillus u yl lqwfaciem BS27, BS2084, 22C-P1, 15A-P4, 3Λ-ΡΑ LSSAOI BS18, ABP278, 1013.918:
Bacillus xuhbHs Ai -' and
P e b Hhis poiymyxa ABP 166
Results of the assay are summarised in fable 4,
CoikPHrich i yq- mink l was inhibited greater than the control (F< .0 1 ) by A. pviy yxti strain A Pi- i"-> \ t - - ;> κοη. I S <■■>:. BS2084, 918, 5sA ,p 22C-PA J.5A-P4, BSI8, 1013,
ABP278, and 8S27. iwardnw gro ineartim was inhibited greater than the control (PyyO.OO I ) by A poiymyxa Strain ABP! 66 and 11 atr oiiquekic ns strains LSSAOI, 22CAP1, P i 8, BS28HA, 15A-P4, 3A-P4, ABP278, BS27, and I3SIS. F arium woegoAoAes was inhibited greater than the ntrol (PoO.OOli by & o yioiiqui'hfcie strains LSSAOI , - 14 I5A--P4, and BS2084, Sckrols kisa rmiorum was Inhibited greater than the eomrol ί pyO.OQ 1 ) by A. poiv-myxu strain ABP 166 and A amybbiquc/ i s strains PI 8, 1 SA--P4, 3Λ- P4, BS2084. LSSAOI, BS18, BS27r 22C4M, 1013, and Λ.ΒΡ278, Cochtiobolus hetersiropi was inhibited greater than the control (P 0.001 ) by P, pofymym srain ABP i 66 and B. yhliq /a ie strains LSSA 1 , 15A-P4, 918. BS20S4, BS27, 22C-PK 1013, BS 18, ABP278, ami 3A-P4. xseroM mruricuinwss inhibited greaiet than the comrol ίΡ<0001 i by . p iy ra strain Λ.ΒΡ166 and A a ykbiq fy le strains BS2 S-4, LSSAOI, 22CAPL 3ΛΑ , 918, oBPiAA P5A~P1 1013, BS27, and BSI8 and t. subtiii strain *B-P5. FmPfwm vsFgi-iH m was Inhibited greater than the control (P 0,00l) by A myialiqiiiqheie ' strains ABP27S, BS27, 220~PL 3Λ-Ρ4, 101 , LSSAOI, and 15A-P4, Diphdi mayd!s was inhibited greater than the control (P<0. 1 ) by A. pofytmwx strain ABP 100 and 8.
Figure imgf000140_0001
strains 9 ! 8, 1 } 3, t. SS AO 1, BS 1 3 A isg BS20S4, BS27, 22C-P 1 ABP278, and 15A-P4. Co hllob lm u>-bo>n<m was inhibited greater than the ::·;·<;···.;
Figure imgf000141_0001
- :m BS2 S4; 918? !,SSA0K ABP27S, 1013, B iil 15Λ~Ϊ>453Λ-Ρ4, BS27, aod 1 ^ -Vy
Tabl 4. Average ¾>ne of clearance in mm averaged over three replicate plates.
TYfsattHSni >;.:,r yatii'aii.'istias ·..>.··'··········->
000i! 0.00ci 0.0Q¾ 0.Q{ 370' 0.00* o.oo* s.oo: O.Oi .67^ 3.00s* 0.33* 7.33 : 833s ■!0.00*:Ii Q.07a's 8.67b
7.33 2.33* 0.00fc 6.33* 6.00SE 11.Q 'i <70i 7.00:: 7,33*
7sr 4.67!! i ¾7a 7 3* 9.67s 13.67s* 2733** 3.37'- 3 ¾" mn 4.00- 0,00" 3 *" 567s 700* 0.00* 8.67"' 6.67*
8S27 3.33;' 2.33* 0.00fc 8.6?¾ 7.67K 8,33fi 4.00¾ S.335 6,00¾
1SAP4 4i>?*;': 3,33s* 1.33s 10.00* a.07* 10.00**** 2.00*" S-071 387*
s.ooabs 3.00s* 03X* 10 OO* . 7* 12.00*°* 3.33* 86"* 6.67* o.ooe Q.0Di! o.oo- 000'* 000* 000* 0.00 o.oo!-
BL.71 0.00" Q.Oi o.oo- 0,00; LOO* 0.00s 000s 0.00i: 0.0QS
3.67* 1.33s 7.67* 3700Λ 1 00si: 100"* g.67p 6.6?"
¾2Cf*1 6.00s* 400* o.ooi! 33:- 6.67* 367aK 3,8'··* Q.00 sis 5.33*' 4.0Q* 10.3Γ 9.37" 11.67^* ί.33¾ 5.77" 8S7B
1013 4.00* .β?* 0,00" S.67" s,Q0fi 10.00** 267* 9 ·?0:' 7.00*
S8-P5 1.00s 0.00d 0.00" 0.00 s 1.00* 56 " 000" 3.00" 133^
<ΰ. οι Q.00? <0 «?? <0.0Q1 ΰ. 01 ·:,' 0Q1 -.0007 <y.00? 0.33Ϊ
060 0« ! ± o ai 1.10 140 .1? 0,73 052
**Tr a«se vvjL In o imte w¾h diii refti suptreonpjs differ, P y 0,00 S,
Sspsm s? of snesnss by Tufey HSD,
St i have ti/nngei effects egaii t pimt-ei.wfchtted fylhi m
MATERIALS A D ETMMJS
Three J Αθ:/η isolates were taned from DtAPonOaSoneer for irrbibhion testing;
Figure imgf000142_0001
s admky, Pyik iv d!sxoiocimt. ana pythi n gnmnnkoia, Pyshium rsobAss were struck to purity onto V8 agar. and then tnmsierred to a 30 nd lass a-o t be containing SO mi Vg broth. The molds were grown at ..···* AS shaking at 1 0 rprn for 7 d ys, She BacCi!w y!olkj fiics ns strains BS22, BS20H 22C--P 1 SA■ P4. 3A- 4, LSSAOI, BSiS7 ABP2A8. 1013, uni 918, Bacillus I nif rwis strains AM2 and BI./2L, BaeiikiS suht-'Hs -\v:nn 3 -P5 and B enibacP! po!ywyy stran \ V 166 wore gr wn to purity by streaking froze culture o 4'ryptlo Soy Agar (TSA) plates. The 1'SA plates ere grown at 32Τ· or 24 hours and used promptly for she assay.
bi.r days <a' ro vth. the Proa aw? salons was: removed A. an the incubator ro sortexed to bresk da bail formation, I Aeg. a sterile cotton swa , the Py-v m was struck onto the surface of a PDA plate three tiroes, turning, the plsle ¾tf between ss'rsskings to obain even moeoiation. Six diJYercnt B cP!us strains were tested on each swabbed plate by picking an isolated colony i'B vil with a sterile toothpick arid stabbing the agar stn:iace. Each PacPl so. da as tested m asphcate. Plates ;vere -nw;S.ii 4 at room wmpw-oar.- tor ί 20 hours; thee zones of inhibition in the fyihia U vo were rneasared and recorded in millimeters i" clearing distance from edge of Bacniiss colony to edge -f ty :Ao--w growth- Statistical analyses was accomplished using GLM proeeduio of SPSS ( version 12,0, SPSS Inc., Chicago, iL). Mean separation as determined using T'akev L!onesth Significant Difference (HSDitest, For •A; analysis performed, significance was declared at P 095.
RESULTS'
These s rains have shown the ability to inhibit the growth of Pyihi Pi vawo:
lAaAAaa i n hliq fa i BS27, BS2084, iSA-P4, a yd'-l. LSSAOi, BS!S, 918;
Ba ii/as Pdienifimr BAS42; BL2! ;
Βαα!ϊ∞ Auviiiis 3&-Ρ5ι and
Paenihacinw poiymrxa A P 166
Results of the assay are wane. ri..ed tn Table 5.
Pyt iu ,>p!<ifi(h aoraAasA s inhibited greater ihse the oonbof by ·' poiy' yxi? strain ABP 16-6 and /;. ybiiq sfdek m BS27 (p ts.001), 15Ad¾L and LSSA0 i , and A i hetdtbrriPi st ains i.21 and BA842, Asa-ana dks io as inhibited greater than the control by P.. poiy yx straits ABPIdb p<0,O0i) and B. ityk g dem strains LSSAOI a ad BS2084, and B. ikheripprmk strains BL2! and BA842. Pyi w! gra nfcok! was inSbmod greater that; the control b . p !y yxa strain ABP 166 (p<0.00n and B. amybBiquefac strains BS1 % (p< .0 n, I5A-P4 p i?.U0i), 918 (p 0.001 LSSAOI {p<0.001g BS27 (ρΐϋΟΟΙ), BS20I4, and 3Λ-Ρ4. « subtWs strain 3B-P5 (pAO.OOig and J? tfcixnifortnis strain BL21 ,
Table 5, Average z »e of clearanc m mm veraged over three replicate plates.
Pytftium
Pyi ks Pyi iu
splmui s
Treatmen sandusky dissQtocum gr ninicoia
Control 0.00b 0.00b O.OO
ABP16S 2.6?ab 8.00a 13.00a
ABP278 0.00b 0.00b O.OOe
L88ACH 0.6713 i.33b 3.00bcd
BS18 0.00b 0.00b 500b
8sa? 3.00a 0,00b 2.67cc!
1SA-P4 2.67ab 0,00b 4.00bc
3A-P4 0.00b 0.00b I.OOde
BAS42 2.00ab 0.33b O.OOe
8102 2.67ab 0.67b
BS2084 0.00b 1.33b 2-OOccle
22C-P1 0.00b 000b O.OOe
918 000b 0.00b 4.000C
1913 0.00b 0.00b 0 OOe
3B-PS 0.00b 000b 2S0cd
P~vafa® <0.0Q1 0.00O <o.ooi
Figure imgf000143_0001
Separation oi ;u-,ou> by Takey HSD.
St im with nmwlotida' ejfix-ts
MATPRSALS A METHODS
Camorkabdi <;Lga N2 worms and feeding bacteria, A. eoO OP5 -1 t streptomycin resistant strain) were parehased from the Caenorhabditis Genetics Ceraer(CGC) at the University of Minnesota. The C. ΟΙΟΪΓΟΜΛ' assay was adapted from See is fd.Af , Pearaseheck M (20t 1), Measuring (.'es rh bditis e/epa«y Lite Span in 6 Well Microliter Pistes. JoV B.49, DOi: i 0.379 j 22496. In summary,. ^kg wov tested at a synchro! ioa i, 1 stage with a concentration of 80- 100 worrasmi and OP50-1 eeding bacteria v added to the worm culttue at a final concentration! f 6 mg/rnl ( 02 x 10" bacteria / mi). Bacillus amyMujutfaekm strains BS27 BS208a , 22C-P \ , i 5 A-P4, LSSA01, BS i 8, ABP278, and 918, and B^ciUm lichettifonnis strains BA812 and B1..21 were o n un Tryptt Soy Agar plates to obtain pure isolated colonies. The colonies were inoculated into test tubes containing 9.9 ml sterile peptone nd! the optical density reached 0.08 - 0.1 at 625 am .>!."·* · s'A;>: :·.·;;- Tins density ofBaciUus cnltnre w s considered full strength and the ouit re was also tested at a diluted rate of B i 0 in peptone. Four wells of a 96- e Si low profile clear assay plate were inoculated with 120 μϊ wonrbOPaOX solution sad 30 μΐ Ba lh peptone culture at either full strength ¾ IX) or at the P iO dilated rate (0XX) for each ea ncra Sterile peptone (30 μΐ) was osed as a negative consrof Tise plate wo. covered with an adhesive plate se l and incubated at room rerrsperatirre.
The surv val of the m population was recorded at 24.48, and 72 boors using a hsvcfted oheroseope, Tise wo n: population n each well was scored as eidser alive ( 1 0%), dwui pp%) or half ahve half dead (50%). Statistical analysis of nematode survival was aceosrpHshed as 2 x 13 tectorial ANOV'A using 01X4 procedure of SPSS {version 1 X0. SPSS inc., Chicago, it). Doe to significant facto* interaction, data wus re-anaiy¾d as one-way A O'VA. Meart separation was detennhied nsng T;Aey Hooesdy Significant Din'crence (HSD; t s , for all analysis perfomwd, significance was declared av P 0.0S.
RESULTS
These strains have shown the ability to induce death as Caen rki dins eleg ns N2 wonaw at fad strength and ai a I : i 0 dilution:
¾w/¾i myio!iijuifackm BS27, 8S20S4.22CT i , 15A-P4 , LSSAO 1 , BS 8, ABF27S, 9 i 8; and B dH nc evifbrmis BX842, BL2 ) ,
The percent of living w nns is recorded in Table 6. After 24 hours I tr atment L5A-P4 decreased the worm population, to 50% (p O,05). After 48 hours I treatments I S A-P , 22C-P ϊ , 271 BA842, BL2 i , BS i 8. B520?i4, LSSAO I ¾ and 0.1 X treatment ? 5A-P4 decreased the population to 0% (p<005 )..1 X treatment 01 B and 0.1 X treatment LSSAO) decreased the population to 38% ΦΧθ Τχ !X treatment BS27 and O.i treatments ABF278, BA842, BSI8, and BS2084 decreased the population to 50% (p 0.0S): and O.i treatment 22C-P1 decreased the population to gg%. After 72 hours IX ireatrnents PIS, $2~L and P.1X treatments 22C-P1. A8P278. 9le: BA8 2, 61..21, BS!S, BS2084, BS27, and LSSAO 1 decreased the population to 0%> (p 0.05).
with B cillu <ύ either Ml siren
Figure imgf000145_0001
Sirai mil? ike ability to kuhice death in Cden&rk bdhis d ms N2 worms MATERIALS AND THODS
Caerhjrhab iiis eleg s N2 worms and feeding bacteria, E. coll OP50-I (streptomycin resistam strain) were purchased fr t e Caenorhabdltis Genetics Center (CGC a the University ofMinnesota. The C assay was adapted from Soils COM, Petrascheck M (20! 1 }.
Figure imgf000145_0002
tieg m
Lite Spas; in 96 Weli Microliter Plates. JoVE.49, DOI; 1 .37 1/2496. hi summary, O. >>!? ms were t sted at a synchronous LI stage with a concentration of SO- ) GO worms/ml and PSoO feeding bacteria were added to the worm cultu e a? a final concentration of 6 mg/rrd (0 x i v bacteria / ml). Bacillus
Figure imgf000145_0003
strains BS27, BS205 22C-P0 I5A-P4, LSSA L BS1S, ABP278, arid Oil Bacillus lictetiiformis strams 8 AS42 smd BL2 ! t Bacillus billls strain 3B-P5 ,wd Paemb: w0«w t/o!ymyxa strain ABP ! 06 were grown on Tryptic Soy Agar plates to obtain pure isolated colonies, fbe colonies were inoculated into test tub s coutaimng 5 η o, ow water until the optica! d nsity reached 0.08 - Odd at 625 nm absorbanee. Four eds of a dS-weil low profile clear assay pla te were iiiocakoed wnh 12 μ! worrn.A9P50- 1 solution :- 30 μΙ /¾«2/0w water culture for each treatment. Sterile water (31) was used as a negative control ' he plate was covered with m adhesive plate seal and incubated at room temperature, ihis experiment was performed twice.
The snrvwai of die worm o ulati n, was recorded at 24, 48.72, and 144 hours asing an inverted microscope. The worm population n eacP wed wa scored as either alive { iOOAo, dead (0%) or Half alive half dead f'50%). Statistical analysis of nematode survival was accomplished as on -way A NOV A blocked by trial using GLM procedure or SPSS (version 17.0, SPSS fnc,,: Chicago, lid. Mean separation was determined us g Tukey Honestly Ssgnifieant Difference (i!SD) test- For ail aoalysw performed, signifi anc was declared at P 0,05.
RESULTS
These wraws have shown the ability to indu e death in Cmniorhaba'tiit eiegans 2 worms;
Bacilfni- amyloii aifa iens BS27, BS2084, .2,2. do 15Λ-Ρ4, LSSA i, BSI8, AP4P2 Ay 1013, v!W
B cUim iichmijhrm BA842, BL21: and
Ba illus subiilis 3B-P5
I !>·.· percent of living worms is recorded in Table 7 A ter 48 hours, treatments .As. i 5 A-F4, BS 1 , and BS27 decreased the orn? population to 50% (pyO.05), treatments BA842 and BS2084 decreased the poptdarioo to ¾3%, treatment 91 S decreased the population to 75%, treatment BL2I decreased th population to 88%. arid ireaoneo! I SSAO 1 decreased r e papn i ion to 94%. After 72 hours, treatment BS 18 decreased the worm population to 0% (.p^O.OS), treatments ABP278 nd 15A-P4 decreased the opul tion to 25% (p< .05), treatments 58, 22C-P1 , BAS42, BL2 L 8S2084, BS27, WKI LSSAOJ decreased ihe population to 50% (p<0052 treatment 3B-P5 decreased the population to β ¾Η and treatment 1013 decreased the population to 75%. After 144 hoursAreaowsnts ΛΒΡ278, 1224· i.32C-P! 8 A 3 2, ana 8L2 ! decreased die worm population to 0% (p 0.05), treatment BS2084 decreased the population xo 13% (p<0.05)t treatments 918, 8S27;: and LSSAO I decreased the population to 25% (p<0.05), treatment ) 0 I 3 decreased the population n.> 63 , and Treatment 3B-P3 held the wo m population to 699;,.
with Bacillus.
Figure imgf000147_0001
j2 MBeEll
Strain* with ike ability to prvm e arigold plant growth in <> MATER ALS AN D METB M)S
Eleven il. a ;.oos strains rested individuall in professional potting mix at dose of l xi 0; CPU/g with a currier of 2 rice hulls and { ? limestones 1 "he control treatment was the poising mis with the carrier to determine the Bacillus treatment effect. The Bacdhis strains used were BA842;: 8L2 K BS2?, BS2084, 22C2P E I SA-P4, 5A d , LSSAO h BS1 8, ABP-27S and ABP- 166,
Each treatment was ansed mto approximately 0 5 em ft, poidng mix and id fed 2S8 sixod plug uays- (5/8" square), which were cat into 3x 12 sections. Eight ma i old seeds (A ntigua Yellow ? were s^wn in the nod If row of each tray. Nine trays were used per .Bacillus heatnseni and 1 -V tray s were ased tor the control treatment. The trays were randomized on a propagation bench with a heating mat set at 72 -'P. A misting system was set two loot a ove the rrays spraying rn a circular pattern for stx seconds every tour minutes from swnr.se to sunset without fertilizer. After 12 days the trays were weaned off misting and moved to the research house without a heating nan. the air temperature was set at 62*F and plants were hand watered with fertilizer.
Seed germination was recorded on day 6 (out of 8 seeds). At she end of the trial (35 days}, the six inside the plants of each replicate were measured tor plant height {'measured in millimeter from the top of the lastic tray to the hig est natural point of the plant), width of dee third true leaf pair (measured in millimeters from Up-to-tip the natural width of the third true leaf pair), bud presence (yes ~ 1 ; no ~ 0). visual root ping rating ( sual radng of one ssde of root plug using, a scale of I -?·)< and. dry weight ( lant cut at s l surfa e, put In Ά sfsa!! paper coin envelops, dried ¾t.55°C tor 3 days and weighed on scale lo 0.01 g t Measurements ww taken durin a 6 I -a, period in which plants were not ered. Any instances where a lait did not gro or a measurement parameter was not obtained n¾re left blank during recording, P m measurements were statistically analyzed try an external statistician,
RESULTS
These shams have shown the ability to promote root formation, shoot height growth of leaves, ioi-snatiors of flower bud;:, and or promote eater dry weighs ;v marigold gfeemxmse trials:
Ba ilii^ my H uif ciens SZi .ll Vi, 15AP4, 3AP4. LSSwOL BS 1 S:
Bacilhts ichmtformis Bill: and
P enihc&Hil pofymyxa ABPl 66.
The data is surnrnari¾ed in Table 8,
Dry weight was greater for plants treat d with 22C-P1 (p-0.062)( BSIS, LSSAOi, and 15A-L4 compared to the control. Height was greater in plants treated with B$ 1 S and22CAPi compared to the control. The naoaai width of the third inre loaf pair was greater in plants treated with ABPl 66, BS 18, LSSAOI , ,.d P L and 15A-P4 compared t the control. There was a greater proportion of budding plants treated with 2 C-F1 p« .003"?}. ALLUA and BS 18 compared to the eontrol, Root ratings were higher overall in plants treated with 22C-P? <p-0.O36\ 1 A-P (p-0. ?2X BSLL 3A-P4, LSSAOi, and BL2i compared to the; control Higher seed genninailon was: present in piataA treated with BSiS and l$Ad:>4 mpared to the control.
Table 8. «ar of all greerr!iouse Ϊ s asirfeir ents -with standard de isi tss is pareo theses. P-val ties indicate a significant df fcrence costo-ared f :· t e con »l tor that measurers K!i!.
Figure imgf000149_0001
(0.0053) (251)
i" 0.026V :-: L : iiiilliiiiiil 4.00 ;CMS) Illilllill:
Iplllfl
BS18 0.0359 36.93 22.91 (5.6S "023(0.13) 4.54 (0,28) 7,89 (0,33)
{0.00635
.: ·'. f -i ΙΙΙΙΙβΙΙ^βΙΙΙΙΙ iiiiii iiiiiiieiiii
liiiliil
LSSAOi 0,0380 35,37 22,27 (5.06) 0.06 (0/08) -'.:!' (u 33! ■'■ ··' (0.71)
(0.0064) (2.49)
·. :- i ll!i!! |lllllill|ll |) |:|il l:
11111 liiiliil
22C-P1 0.0406 38.00 22.74 (7,87) 0,35 (0,52) 4,72 (0,23) 7,33 (0,71)
Figure imgf000149_0002
in 0,0293 32,06 19.37 (5.40) 0.02 (0/07) 4.36 (0.63) 7.5669.53)
10.0060) 2,94)
EXAMPLE 12
St ,ί,ν wUh th $ ίώίϋί prom&te > OOifh MiitiaSi
MATERIALS A m M H D
! hree Bacillus strains ·.· ·■:■ •idlvidually, paired, a? td all three combined in prol s 5K>nal posting m ;s a total t -xtatment dose · -f ) s ao? CFU ;'g with a ·■. ;';:":':·. :' f 2/ i rice hulls and i/3 limestone . The eofstroi treatment was he pmting mix wii ? the card er to determine t e B viiins ϋ ·. iil ·": : effect. Thc/i ¾3ii-i.s stf&hss ttsed were 22C -1*1, I5A-I »nd £ SIS,
Bach t :¾ati*neni was mixe^ 1 im pf foxssaatviv 0-3 CEI, it. potting mix aoc tilled 288 so plug tays
(5/8" square) \ vhsch ere c t into x!2 sec turns. Eight narigoK ! seeds (Antigua Yellow) e; e sown in the mi dle row of aach tray. Nine tm j's were « set! per Bacillus treat : :·.·;;! and : 8 it .0 s vvere used t ;· the control tr¾ainie«t, Th- ί iravs were randos nized on a propagation bench wili a heatkg r 'sat set at 7 * F. A misting system was set two feet above the trays spraying in a circular patera for sis seconds ovary four auntnes from sun ise to oaoe; selthosu tertdiKer. After 6 days the trays were weaned off misting and hand watered v oh light f rtilis r.
At the end of the trial (33 days), the six inside plants or each im licate were randomly measured tor bud presenc (yes ::: I ; no Oy visual root ping rating (visual rating o f one side of root ping using a scale of I - S), and dry weight (plant cut at sod surface, put m a sm ll paper coin envelope, dried at 55''C for 3 days atid weighed op scale to 0.000 !g). Measure-mems were taken Pa i g a 6 hour period In which plants were not watered. Any instance where a plant did not grow or a mea.su remen? parameter was ;e obtained wars lest blank during recording.. Plant megs emems ere statistically anal se by an external statistician.
ESULTS
These strains and combi ion of strains have shown the ability to promote root formation, ioonation oi flower buds, and/ or promote greater dry weight in rnarigold greenhouse trials:
oi.. A.-
Figure imgf000150_0001
set Pi. IsA A BSIA and
Combinations: S iiim myhl / aciem 22CF1/1 P4; 22C-PS/SS18; 15A-P4/B$lS. :4s. darn is summa ze in Table A.
There was a greater proportion of budding in plants treated with combination treatments 22C- P!/l 5A"P4; 22C~ 'I/BS 18y and 15A-P4A5S 14 compared to the control. ROOT ratings were higher overall in piano treated with couAination ireatmen 22C--P1 15A-P4 compared to the eonboS. Dry wei ht was greater for plants treated with 22C-P 1 , 15 A-P4, BS 18 (p-ALOGA) rid combination treatments 22C- P1/15AA and 22C-PI/BS18 compared to the oonSroi,
Table 9; Mean of all greenhouse easuements with standard devori rrs in parentheses. P-vaiues
Figure imgf000150_0002
22C-F'1/15.A- 4/BS1¾ 0.820 44.1 '■ (OAS) o.i?; (0.02?) MA TERIALS A METHODS
Three I i iihn strains were tested individually, aired, and ad dues combined in professional porting rate at a total treatment dose of 1 x10'" CfU/g with a carrier of 23 rice bulls and 1 /3 limestone, lite comroi treatment was the pottmg ml.x with the carrier to deterrn ine the Bacillus treatment ef%et. The Bacillus strains used were 22CAP 1 , i 5 A-P4 and BS ί 8,
Eac treseimem was raked into ap ro mately 0.5 era ft. potting mix and til loci 288 sized plug tray i /H' square) which were cut into 3x8 secti ns . Nine marigold seeds (Bomn:m Orange) were sown in a checkerboard pattern oboe trays were used per Bacillus treatment and I S trays were used for the control treatment:. The trays were
Figure imgf000151_0001
A misting system was set two feet above the trays spraying in a circular pattern tor six seconds every four minutes from sunrise to sunset without fertilizer. After ) days the trays were weaned off misting and plants were hand watered with hght fertiliser.
At the end of the trial (45 day i, ail rune plants from each replicate were randomly measured tor vegetative plant height ( measured in millimeters from the top of the plastic tray to the highest natural leafs, flowering plant height (measured in millimeters from the top of the plastic tray to the highest point of the flower), flower stage (see FIG , 5} (ranked I A based on bud I : tight bud, 2: Xsael-wd ' bud, 3; " ψ' of color, A folded petals.. 3 ; open fiower/eornplese), vegetative dry weight (plant cut. at soil surface and flower bud removed at natural indentation of formation, remaining vegetative plant put in a small paper cola envelope, dried at 35aC for 3 days and weighed on scale to 0,0001 gy and reproductive dry weight itlower bud that was removed own plant was put in a small paper coin envelope, dried at 55A2 for 3 days and weighed on scale to 0-0001 g). Measurements were taken during a 6 hour period in which plants were not watered. Any instance where a plant did not grow or a measurement parameter was not obtained was left blank during recording. Piunf measurements were statistically analyzed by an external statistician.
II.EStJL.TS
The following strains and combination of strains demonstrated the ability to promote plant height, ilower score, vegetative dry weight, and/or reproductive dry weight in marigold greenhouse trials:
Saci!!m cmyioHqr /aciem 22CF ! , i 3 Α Ι Ί . BS s 8: and
Combination,.: /Α, :/Α:Λ ar y ii ifacic 22CIAA 5A PT 22C-P 1 /BS 1 S;
22C-f' i /1 5AP4/B5 i 8.
The data is summarized in Table J 0,
Vegetative height was greater in plants heated with BS I $ and combinat ion treatments 22C-P1 /1 5 - P4 and 22C-P i/ S 18 compared to the comroi flowering plant height was greater so plants treated with 5 i 8 compared to the comroi. Flower stage was more mature in plants treated with 22C--F I and I 5A-P4 and eotnbms i n tres mems 22G-PF'B$ 18 and 22C-P ; di 5A -P4/BS I 8 compared e - the corrwoi, Ve tat e dry we h wee; greater for lan s treated with BS i ¾ run! consbinadon treatment 22C-P 1/BS 1 X compared to she control. Reproductive dry weight was greater Ibr plants treated with 22C-P1 com ared to the comroi.
Figure imgf000152_0001
Strains with the (ability ta promote greater ve^eiative fresh weight
AT RIA LS AND ME -mom
Six Bacillus strains were wwcd individually us professional potdag m ix at a dose of i x l O €FU/g with a carrier of 2/3 rice hulls sad i/3 limestone. The control treatment was the potting mix wit the carrier to dete ut e the Bacillus treatm nt effect. The Bacillus strains used were BA842. 10 2 i ; BS2084c 22C-F ! , BS I S nd ABP-27S.
Bach treatment was mixed hue a ximatel 2 ou. if potp ig nnv aad idled 128 ized plag trays (3 4" square). Four marigold seeds (Antigua. Yellow) were sown in four alternate rows of each usry tor a total of 16 seeds per tray. Three trays were used per Bacillus treatment and three trays were ased i r the control treatment. The trays were randomized on a propag&Tion bench with a heating mat set at 72Jf . A misting system was set two feet above the trays spraying in a circular pattern tor six seconds every lour minutes from sunrise to sunset without terdiwer. After 47 days Use e ays were moved to the research house., the air temperature was set at 62'-'F, am lants were hand watered with fertilise*.
At the end of the trial (63 days ), 9 plants per replicate were harvested at the sod level and measured on a scale to 0.0 I for total fresh weight vegetative weight, and reproductive weight ( flower bud). Measurements were taken during an 8 hour period in which plants were not watered. Any iusoxeees where a plam did not gr w or a measurem nt parameter was not obtained were left blank during recording Plant measurements were ¾tat iie-t»|{y analysed by an external statistician.
RESULTS
These strains have shown the ability co promote greater vegetative fresh weight, reproductive f esh weight, and overall fresh weight in mangold greenhouse trials:
tkicii!us
Figure imgf000153_0001
B$208T 2CPI , 8 18.. ABP 78; and
Da iim lichmiformis AS42, Bl.2 i ,
The data rs summarized in Table 1 I.
iota! fresh wei h* was greater in plants treated whh BL2K BS20W, 22C--PL Pwl w nd ΛΒΡ278 compared to the control. Vegetative fresh weigh' was re t r in plants treated with BA842.; ΒΪ..21 , BS2984,
22C2-P i BS 18, and ABP278 compared to the control. Reproductive fresh weight was greater in plants treated with BL.21, BS2084, 22C-P1, BS18, and ABP228 compared to the control.
Table 11, M gr f efl greenhouse- measurements,
Treatment Total Fesh Vegetative Fresh Reproductive Fresh
Weight fe) Weight (g) Weight ¾)
rol Sa iP 'i 22
Figure imgf000153_0002
a.s$ 2.23 2. SO
SSIS S.SS wo; 2,68
ii.2 .023 __=≤.
iMELEJS
Sira ss wih the abtiifv to pronwte et formation, shmrf heigh, gnmih ef leaves, ami dry weight i tomato
MATERIALS AN» METHODS
Ph.- we: /w,w/;V. strains wee tested individually in professional potting mix at a dose oi'ialO CFU/g with a carrier of 2/3 rice hulls and 1/3 Umesto»e. The control treatment was the potting, mix with the carrier to determine the Bacillus ireaimem effect. The Bacillus strains used were BA842, BL L BS2T BS20S4, 22CTPP, 1 A-P , 3A-P4. LSSAOB BSiS, ABP278 and ABP166.
Bach tre tment was mixed into approximately 05 ca, IT potting mix and filled 288 sored plug trays (5/8" s uare) hich were e into 3 12 sections. Eight tomato seeds (Better Boy) were sown in the middle ro of each tray. Nine trays were used per Bacillus treatment and 18 Pays were used for the control treatment The trays were randomized on a propagation bench with a heating mat set at 72CF. A misting system was set two feet above die treys spraying in a circular patters? for six seconds every four minutes from sunrise vo :■■.·■■; o; without fertiliser After 8 days the trays were weaned off misting and hand watered with
Figure imgf000154_0001
At the end of the trial (20 days), the six inside plants of each replicate wore randomly measured for plant height (measured in rniiiimeiers from the top of the plastic tray to the highest natural point of the plant), loaf length (measured m millimeters, the first true loaf from the stem to the lip of the leaf), and dr weight (plant cat at soil oalo o. put in a stnai \ paper c ls e elo s, dried ar 55 A3 for 3 days and weighed on scale to 0.0001 g). Mo ;■:·:■■■:>··.:··■■.<.■.■ were take;; during a 6 hour peri In which lain were not watered. A ay instances where a plant did not grow or a m asurement parametet was not obtained w e loft blank during recording. Plant measurements were statistically analys¾d by m external statistician.
RRStJLTS
I a. r . strains have shown the ability to pronsote shoot height. growth of leaves, and/ or promote greater dry weight tomato greenhouse trials:
Bac&us myioliq ifycien BA3A BS2", BS2084, 22CP I } 15 A Pa, 3 AP4, LSS u 1. BS 18, ABP278
Bacillus ikheniformis BB21 : and
faenibacilius poiyrnyx A 1 6
The dat is summarized m Table 1 ,
The height w¾s greater in plants treated
Figure imgf000154_0002
BA842 (p:A).00i): BL2I (p-0.003); BS i 8 ip:::0.008), :;.A AA (p-0,009), LSSA01 ("p=== .009); ABP166 (p ).013)t 15Λ-Ψ4 p-0.023y BS2084 (p-A024g 8S27, 3A-P4, and ABP27¾ compared to the control. Pts length of the first true leaf was geater m plants treated with BS20H4 fp-0.00n. BSIgi - .OPty BL21 tp-0,017y BSSA0! ( =- .i>23). BA.842 (p= .02 A, BS2A a A- P4, ABP27A and ABPtAd compared to the control. Dry weight was greater tot plants treated with BS2084 fp-O.OOS), BL2i ( =O.G09)f LSSAoi (p-Q.01 ! g BA842: BS2 ", A V I, and BS! 8 compared to the control
theses, P-valoes
Figure imgf000155_0001
Stmifi-s with ike ability ίβ promote plant height, gm k of leaves, promote root fi naihm ami/ at promote g eater dry weight in tomato greeakome trials
MAT £ RIALS AN M nso&s
Three Baculus stra ns were tested individually in professional posting rok at a dose of ! x I 0; CFU/g vridi a carrier of 3/3 rice hnHs and 133 limestone. The control treatment was bw potting mi with the carrier to determine the Bacillus treatment effect 3 be Bacillus strains used were ol d: ! BSeOiwg and LSSA0 L
Figure imgf000155_0002
into approximately 0.2.5 eu. ft. potting mis and filled 288 sized plug trays (5/8" square) which were cut into 3 2 sections. Eight tomato seeds (Beiter Boy} were sown in the middle row of each tra s Nine trays w re used pes Baciliw mmmvttt and 18 trays were used for the comroi treatment. The days were randomise on a. propagation bench · · :b; a headng mat set ai 72"F. A mssting 1 <em was set two feet above 'he trays spraying in a circular pattern for six seconds every four minutes from «snri.se to sunset without fertilizer.
At the end of the trial (311 days), the s inside plants of eac replicate were randomly measured for lant height (measured in millimeters (torn the top of the plastic tray to the highest natural point of the plant), leaf length (measured in millimeters, the first true leaf from the stem o the tip of the leaf)., visual root plug raring t v i ual rating of one side · ·; οοη plug using a scale, of I --5), and dry weigh (plant cut at sod surface, put in a small aper coin envelope, dried at 55w5 for 3 days and svesghed on scale is- 0,0001 g). A ny instances wher a lant did noi grow or a. measu ement arameter was not obtained er left blank (i.*nng recording
Plant snessumiieofs were statistically analysed by an external statists iao.
RESULT
These sudns hove shown the abdriy to promote plant height, growth oi' leaves, promote : · ?· ·;. formatio and/ or promote greater dry i ht in tomato greenhouse trials;
ikieiUm amyhilqum em LSSAO I ; and
Bacillus iickentfbrmh Hi 2 i
The dais is summa ized in 'T able 13.
Height was greater in plants truste with BL2 i s p% -008g BS20S4 i -0.0 ί 7). and LSSA01 (p- .i-O ) compared to the control Length of the first free leaf was greater in lants treated with B1..2 I . BS2084, and LSSAO ί compared to the control. Root raorsgs were higher overall in plants; treated with LSSAO 1 (p;;;0,03 ); BL2 L and BS2084 compared to the control. Dry weight was greater for plants frette with BS20S (p"-0.Q62)» BL L and LSSAO 1 com ared to the control.
Table 13; Mean of all greenhouse measurements with standard deviations lit parentheses. P - vaitjcs indicate a significant difference compared to the control for feat m asurement
Figure imgf000156_0001
ΜάΜΜύϊ
Straim shown ΪΟ pwmoie seet htg germination
MATERIALS A ND METHODS
Three Ihciiim strains 22C--P L 1 5A~Pd, LSSA01 , were tested in a e-orrs seed germination assay. Speculated spray-dried material of the facU s strains were diluted in sterile peptone to a concentration of i o l O" CFU/mf. Sterile niter prmer (Whatman 1 ) was transfene to the bottom of sterile disposable petri plates and soaked with either 10 mi sterile peptone or 10 ml of the diluted Bacillus product. Com seeds were urch sed from a local ga den center and disinfected with 70% Ethanoi for two minutes, rinsed wish sterile water, soaked in 20% bleach for 10 minutes, and rinsed twice with sterile wafer. Disinfected seeds were allowed to soak in sten!e water tor 20 minutes, and then 10 seeds were placed on the soaked filter paper for each treatment. Treatments we e tested in triplicate- Petri plates were covered with a 5 cm tail glass dish a id one plate per treatment as placed randomly on one of three shelves, of a germination chamber. Use germination chamber w¾s set for fight period of ¾ hours si 29.4*C ;«?<! 65% relative humidity and dark period of 16 hours at 23.9A7 nd 50% relative humidity.
At 16 hours the 10 seeds ift each plate ere recorded for germination and repealed every 2 hours until 24 hours. Seeds were allowed to continue to grow for 5 days. lter 5 days she geminat d seeds front each replicate treatment were emoved and placed on a pre-welgaed aluminum pan gnu dried at 55'C for 48 hours. Dry weight measurements were recorded and the average seedling dry weight for each replicate neatrnent as calculated.
RESULTS
These strains have shown the ability to increase the germination rate of corn seed in vitro:
Baclil anry i ufyci m 22CP P J 5AP4, LSSAO I
The percent germination rate for each treatment is shown in FIG. 6. At \ 6 and f & hours, ad three treatments bad a higher germination rate than the control seed, but by 20 hours there was no difference in the rate of germ in at ion. The average seedling dry weight for each treatment Is shown in FIG. 7. After 5 days, tr atment 22C~P i produced seedlings with the highest dry weight, followed by I 5A-P4, LSSAOI , and control having the lo est average seedling dig weight.
ΈΜΜΕΜΜ
Strains with th ability to promote growth in y beam.
MATERIALS AND METHODS
Bacillus ylonqmj' cle strains, 22CF1 , 15AP4, and LSSA0 I were coated individually and as combinations thereof on ten varieties of soybean seeds to a final connr of \x ! f ; CPU/seed. At eac h of five geographical locations, two varieties w re- tested in four replicate plots each. Piois were designed as 4 rows with 30" spacing between rows and each row was 7 ½* long. The same variety of seed was left untreated and tested ollowin the same design to be used as controls. At S SO days after planting, plants were scored for emergence-, stand (plantsfoio?), and seedl ing vigor,
MESPLFS
Expected results wail show that so bean seeds treated with Bacillus a yloiiq efucie strains 22CP I . I 5AP4, LSSAO P a«d combmatloits thereof showed an impr ement for emergc-isce. stand, and seedling vigor, on ail vanebes treated compared to the control seeds of the same varieties.
Strains with the ability to promote mriy vigor, stand, ami igher i/veraM el In com:
MATERIALS ANP METHODS
Bacillus mnioi iiiefacie strains. 22CP P I 5A P4, and LSSA01 were coated individually and as combinations thereof o» one hybrid of traditional corn seed at a final count of 1 x 1 if CFU/seed. Seeds were l nted ·:·> three geographical rww- ns and tested in four replicate plots each. Plots were designed .- 4 ross it 30" s acin See won rows and esc row was 7 ¼' long, lire same hybrid of seed was left untreated and tested follo ing the same desi n to be used as control. At ISO clays after planting, lains were scored for emergence, stand {plargs/plot y and v-ilnie vigor.
RESISTS
Expected resells will show that com seeds treated with Bacillus• fmyloiig efaciem strains 22CPK J5AP4, LSSAOK and combinations thereof, showed an Impro ement for emergenc , stand, and seedling vigor, o sii varieties treated compared to the control seed of the same hybrid,
EXAMPLE 3D
Strains with the ability to promote early vigi*rf stand, a higher overall yi d in drought tolerant com MAT RIALS A.N» Mm BODS
Bacillus amviOiii ie icie strams, 22C2PL ! AS Al arid LSSAOI were coated iodividuaily and as combin ti thereof n four hybrids of drought kderantcswn seed at a final corrrd ol A h CPUAeed. Seeds were planted at throe geographical locations and tested in four replicate plots each. Plots were desgned as 4 rows with 30A spacing bet -wen rows and each row was ? ½' long. ! iw same hybrid of seed was left untreated and teste following the same design to be used as control At 180 days after planting, plants were scored for emergence, stand (plants'plot). and seedling vigor.
RES LTS
Expected results wbi show ;h u corn seeds treated with BacHluc, Vfpeo iWbow??s strains 21s hi 15AF4.. LSSAOK and cotobmatiori thereof, showed ·»· m- rt einem >■■;■ emergence. Wi;.j. and seedhng vigor, on all vanebes treated compared to the control seed of the same hybrid,
Strains mtft the ability fa promote growth (yield, stand, early vigarj I wheat ATERIALS A EJ METH DS
B Jia a yhiiquekicien strains, 22CPL 1SAP4, and LSSAOI were coated individually and as combinations thereof o two varieties of wheat seed (hard red winter and sob red whiter; at a final eonm of Is.10" CPU/seed, Seeds were planted at different eograp ical locations aid lesled in four replicate plots oach. Plots were designed as 4 rows with 30"'" spacing between rows and each row was 7 A' long. The same hybrid of seed was led ontreaied and tested
Figure imgf000158_0001
-.ama design to be tssed as cosUrob At I 80 days after planting, plants were scored for emergence, stand (plaotspiot), and seedling vigor.
RESULTS
Expected results will show that wheal seeds treated with Bacillus u wioiMii heiens strains Ad hi , 15 AN, LSSAOK and combinations thereof showed an improvement for emergence, st nd, and seedling vigor, on all varieties treated compared to the control seed of the same variety. Baalim mnfhMquifitci m 22CP1 promotes e&riy vigo , stan , am higher overall field
MATERIALS AND METHODS
acili a y liq fha'e strain, 22CPL as coaled on corn -seeds to a final count of Ixl i CFLPseed along with standard seed tre mssm obemisines. At each of twenty geographical loca!ionss two hybrid', were tested in four replicate plots. Plots were designed as 4 rows with 30" spaaing between rows- an each row as I ?. long. Seeds of t e ame hybrids were treated wit standard seed r.eatwesa ehennstries bus without, t e addition oi 22CPI for use as controls and tested to! lowing the same experimental design described above. Plants were scored for early seedling vigor ( I -9 scale■■■ ah I -lowest; 9::-highe t) and .aswl :.:<■■:<>: iplants/plot) at the vegetative state V 3 · V i aid yield (bushels/sore} o a -. d termined si. hull fmnn ay of the pi HKts.
RESULT*
InC'eases were seen HI stand count nd grain yield in l ins grown from seed treated with 22CPI compar d to plants grown fr m ('Control seed eslandard seed treatment chemistries but withoai ihc addition of 22 P1). Results are shown in table !·:.
Table 14; Mean of al ratings with Least Square Differences (LSD) listed below each
Figure imgf000159_0001
Five locations were indicative of early stress and plants from those locations were separated for comparison between treatments. Early seedling vigor was higher in plants grown from seed treated wuh 22 P1 compared to plants grown Irons Control seed (p:;:0.1 Of !n addition, here were numeric increases for stand count in pianos grown om seed treated with 22CP1 compared to plants grown from Control seed, Results a e shown in Table 15.
Ta le f.5: Mean of ratings at five early stress locations with Least Square Diffe ences (LSD) listed below each saeasaremersf parameter. BaeiOux emvMiquifackns 22CP1HSAP4 cmHmiikw prm s
rfy grawfk m higher memii yield
MATERIALS A O s HODS
Bacillus anv ikr fbcie strains, 22CP1 and 15AP4? were coated together on corn s eds to a una; count of Ixli CPU/seed alon wdh standard seed treatment eheniistrks, .At each of 14 geographical locations, two hybrids were tested In sk rephcaie plots each, Plots were designed as 4 rows with 30" spacing between rows and each row was 17,5s l op Seeds of the same hybrids were treated with standard seed treatment ehennsmies
Figure imgf000160_0001
without the addition of 22CP 1 H 5AP4 b use as controls and s ed following the same ·οοο π>ο·.·η; d design described above. Plants were scosed for early growth ( I -'·· s al w hh r-lowesp -highest} and stand connt (plants/plot) at the vegetative state V2-V4 and final stand (plams aere) and yield ibushels/acre) were determined at fuU maturity of the plants.
RESULTS
The e ages of both treatments for ach measurement parameter are listed in I able I 6. An increase as early seeching vigor was detected in plants grown in the presenee of 22CP PH SAP seed treatment compared to Control ( p-dd O). There were no differences noted in early stand or final stand count. Pinal yield showed a numeno increase of 0.7 htwhelsAscre in the 22 CP ! Ή 5ΑΡ4 treated plants compared to the Control treatment (standaod seed treatment chemistries but withont the addition of 22CP i e 1 5 AF4).
able 16; Meats of all ratings with beast Square Difference i LSD ) listed below each measurement pa rat i to tor if ign ifka at .
Figure imgf000160_0002
Bacillus mnphliqmfiic m strains, S2CP1, 1SAP4, ami LSSA&l strains prottwie
early or, w ds a d higher owrali yieid
MATERIA LS A D METHODS
Bacillus i yloiiqmfade strains. 2CPL I 5.AP4, and LS3A0 J were coated individually and strains I SAP4 and I. /SSA I svere combined and seated together on two s ariebes of soft red winter wheat seed at a final dose of 1 10" CRAseed. Ail seed was coated wah a c mrncve l standard seed meatmen! and raw treatment inc hided the standard seed troa tneat without a biological component to be used as a control (S'f Dp Seeds were planted at four different geographical locations and tested In s replicate plots per location. Plots were designed as 18" long and 6- to 7» inch row s acing. Early vigor store (i-9 scale wkh Ldowesu H"iighest) ami early stand eonni were sieordsd as well as yield at the c nclusion of the bk-i.
RESULTS
Early vig«_,r results were only available for two of he four locations and are sh wn in J& k 17. An increase \» early vigor score was delected at Poseyvi!le, IN with all treatm n s, 22CPL 1 SAP4, LSSAOl and the combination treatment 15AP4+LSSA01 compared so the STD treatment alone. Overs?!, early vigor was highest when treated with LSSAOl compared to the STD treatment alone when averaging both locations.
Figure imgf000161_0001
Stand resnhs weie only available fo the Atlanta. IN location ami are shown in Table 18 An increase in plants per ¾ere was detected with ireatnwnts, 1 ΛΡ4 and LSSAO ! compared k; the STD treatment alone.
Table 18: Average stand (plams/acxe) at Atlanta, IN- Least Square .Difference (LSD) listed below.
Figure imgf000161_0002
Yieid results tor all lour locations are shown in Table )9. Yield was improved in Atlanta, IN whets seed was treated with 15AP4 and LSSAOL it) Mexico, MO when treated with 22CF1 and I5AP4, and in Posey vii!e. ΓΝ when treated with LSS.A01 compared io die STD treatment alone. Overall, yield WH greatest whes treated with 15AP4 compared to the STD treatment alone when averaging all four locations.
Figure imgf000162_0001
EXAMPLE 25
Baellim aniyhtiquifyde 22CP.I p m te early vigor, stand, and
higher overatt yield ave the cmrse of two planting seaions
MATERIALS A METHODS
Ba d! mykdkHj facieris strain, 22CP 1 , was coated on two varieties of soybean seed at a final dose of I A- 1 0" CFUweed along with a Rhnobiam moculam and standard seed treatmen cherncarses. Another treatment included the Mi >bium itiocttiant nd standard seed treaimera chemistries without the addition of
22CP i (STD) and an additional treatment did not include any biological or chemical seed treatments
(Control). Seeds were planted at 20 diffe ed geographical locations in 2013 and 16 different geographical locations in 2014. The 2013 trial included four replications per location, while the 2014 trial included six replications per location. Row spacing for both he 2013 and the 2014 trials was 30- -inches at all locations and row length was between 1 5 and 17,5 feet depending on location. Early seedling vigor score (1 -9 scale with
D owest: 9;::highe¾t) and early stand count {plams/acrej were recorded as wed as yield (bucbeis/acrei at the conclusion of the trial.
RESULTS
Early seedling vigor was collected at 20 test locations over th course of two planting seasons. Plants grown in the presence of seed treatment 22CP ! had an overall higher average seedling vigor score compared to the Control and STD treatments (Table 20), The 22CF 1 seed treatment also produced a higher seedling vi or ¾0 . e in S ¾ of locations compared to the Con trol treatment attd in 70% of locations compared to the STD treatment over the course of two planting seasons (Table 21 ),
Table 20: Average seedling vigor score Π-9ί for all treatments over the course
Figure imgf000162_0002
Table 21: Petcsnt of locations in w c the 22CP1 ireatmeni had a higher numerical alise for seedfmg vsgor s-. ·. ·:' : c mpared ;■· > Control an STD treatments.
21)13 H K S M¾ 63.6%
Z 4 Q ax 0% Ό7 Χ¾
ear average
Stand count (plants acre) w s collected at 36 test locations over the course oii o planting seasons. Plants grown in the presence of seed treatment 22 P 1 had an overall higher average sianci count compared to the Control and STD treatments in 2013. and compared to the Control treatment in 2 1 (Table 22}, The 22CP ! treatment increased the number of plants/acre a? 66,7% of hseatsons compared ΐο the Control treatment over the course of two planting seasons:, however, this was not enhanced compared to the STD treatment Π able 23).
abh i.c: s cooscc stand onru (plants/acr j tor ail treatments over the urse
Figure imgf000163_0001
'Table 23: Percent of locations in which the 22CP1 treatment had a higher numerical valne for stand eour
Figure imgf000163_0002
Overall yield (his/acre) was collected si 33 test locations; over the coarse of two planting osasona. Plants grown in the p e ence of seed treatment 22CP1 had an overall higher yield compared to the Control and SI D trestnseats (Table 24) The 22CP 1 seed treatment also produced a higher yield in $ ! .$% of locations compared to the Control treatment and in 57.6¾ of locations compared to the STD treatment over the coarse of two planting seasons (Table 23).
Table 24: Average yield (ba/acre) for all treatments over the course of 2 years.
Isilllliilliiieil • · : . : : -■
E014 Table 25; Percent of locations in which the 22CF treatment had a higher numerical
Figure imgf000164_0001
EXAMPLE 26
Bacillus mm iiUifit ns 22CP1 ktne d kt cmnbm tkm with ISAP4 ptvmotes
e&rly vigor, stand, rnt igher overall yield
MATERIALS AND METHODS
Bacii!us c ylidiq ef iens strain, 22CPI was tested individusslly md combination with Baciii arrwioikp d ciens strain, ] 5AP4 on two
Figure imgf000164_0002
! 1 !" CPU/seed along with a standard $ee ireatmettt. Another treatment included the standard seed treatment without the addition of the- biological inocuiant (STD) and an additional treatment did not include any biological or chemical seed treatments (Control.!. Seeds wee lant d at 16 different geographical locations and included six replications per ioeanon, Row spa mg was 3 -inehes at all locations and row length was between 15 and 17.5 foci depending on location. Early seedling vigor score (1-9 scale with Cdowest; ^highest; and early stand count (plants/acre) were recorded as well as yield (bo/acre! at die conclusion of the trial.
RESULTS
Average seedling vigor score, stand count, arid yield are shown in Table 26, Overall, the STD seed tre tme t performed better than ihe Control treatment in all three measurement parameters. The- 22CP1 treatment performed better than She Control reament i ail three iaease-rement parameters and better than the STD tr atme t in seedling vigor and yield. The 22CP1 + 15AP4 treatment performed better than both the Ciaits' l and STD seed r atments in all three measurement parameters,
Table 26: Average seedling vigor score (i-9?, stand count spianis'acte and yield (bn/acre) ibr each treatment.
~, . t . See¾I!iit¾ Vigor , ... ,
f rsatweiti „ taad nst YivM
en re
STD S-4 115,378 64,8
22C ii"lSAF4 5,6 I18,3A> 65,5
The percent of locations at which 22CPI and 22CPK15AP4 treatments had a higher numerical value over the Control ireatinem are shows in Table 27, Seedlng vigor was recorded at locations and both the 22CP; seed treatment and the combination 22CPH-15 P4 treatment gave a higher score at 88,9% of locations. Stand conm was recorded at 16 locations and the 22CP1 seed treatment increased pktrrtsAers at 75.0% of location* rid the c mbination ..'...'*' P i o i 5AP4 treatment increased plamsdaon? at 93 8% of locations. Yield w¾s meoixled si 1 loeaoons and both the 22C I seed treatment an the eombmsuon 2.2. id · i 2 A P4 treatment roduced more bushels/acre at 7- .6¾ of locations.
numerical
Figure imgf000165_0001
ί he pereent oi loc tion at which 22CP 1 and 22CP !■· I 5AP4 treatmen had a higher aeown A; value over the SID treatment are shown in Table 28. The AT b i seed treatment gave a highe seedling %gor score at 77.8% of locations and the combination 22CPI-H 5AP4 treatment was higher at 55.623 of locations compared to the STD treatment The stand count was not enhanced with 22CP ! atone compared to the ST'D treatment, however,, the mbination 22CP H- 15AP4 treatment increased plant Aacre at 68,8% of locations. Yield was not enhanced with 22CP1 alone compared to the STD treatment; however, the combination 22CP H I 5AP4 treatm nt produced more bushels/acre ai 78.6% of l ations.
noraencai
Figure imgf000165_0002
l2T l2«112
Biicillui; <myloiiqmfac 22CPI okme and m emhim&m with 1SA P4
pmmw s earfy gttm>tMs earfy uni, ami yield
MATERIA LS ·, Ν;> METHODS
Bacillus
Figure imgf000165_0003
st ains, 22CP I and i a APT, were coated imjividuail and also together on inbred corn seeds io a fi l count of I x 1 if CFU/seed along with standard seed tre tment chemistries. At each of 6 geographical locations, two inbreds were tested in strip icas with six replicate plots per location. The same inbved seed was treated with the standard seed treatme t chemistries but wWwat the addition of the biologseids for use as controls and tested following the same experimental design described above. Plants were scored lor early gro th ( I -9 scale), early stand eoont (%). and overall yield increase 0¾ » c m ared to the control seed. RESUL TS
! η· results for early r wt s early stand ooam, and overall yieid increase compared to control are shown Table 29. Early growth, eerfy stand count and overall yield was improved in plants grown in the presence of 22CPL 15AP4. and die combination 22CP1 H 5AP4 seed treatment compared to control.
Tsbk 29: Mean measurements recorded tor early growth, early stand count, and overall yield for ah treatments.
Early Growth Early Stawt Overall T k\
Coast (%> (% ifferenc owr
v-utnd ?
$7 : " oyyic c:y]::yciyi: ::'
53 87.2% 2,0%
22CP1-H 5 F4 5.4 86.4% 1 .0%
Although specific enibodioients have been illustrated and described herein, it will be appreciated by those of rdinary skill In the an that any arrangement that is calculated to achieve the same purpose may be substituted lor the specific embodiments shown. Tibs application is intended to cover any adaptations or variations ihai operate according to dse principles of the disclosure as described.
Therefo e,, it is intended thai this invention be limited only by the claims and the equivalents thereo The disclosures of patents, references and publications cited in the application are ineoiporaled by reier^nee in their entitery herein,

Claims

1. A composition comprising ai least w bacterial strains selected ifom Use group con isting of Bacillus ana-io!iquif viiifi B cillus cheruforms, Baalim mbtilis. and Baeranacilius polyim ao ϊτ. an effective amount to achieve an effect selected fern s e group eotisisihig of: inhibit a plant path gen, improve plant erformance, improve plant yield, improve lant vigor, increas* phosphate availability, increase production of a. plan hormone, increase stand count, Increase root formation, in rea shoot height « a plant., increase leaf lengt of a plant increase fltw bud formation of a pla increase total lant fresh weight, increase total plant dry weight, and increase seed germination.
2. The composition of Claim !, wherein the composition further comprises a bioconirol agent selected horn the group co sistin of bacteria, tangL yeasg proozoaw vi uses, emo opathogenie nematodes, botanical extracts, proteins, secondary metabolites, and itmc-cui&nis
A The composition of Claim i, wherein at leas? two strains are selected from the group consisting of: BaciikiS a BBiqiiii cicus BS2? (NRRL B i 51 Ba ! his a^ iq i/ac^s BS2 S (NRRL B- 50013), a l s amyloil ufcicBm I5.AF4 (ATCC PTA-6507), Bacillus a yhiiquBach-m AP4 (ATCC FTA-6S06f BccB!m omykUiquifaelens LSSAOd (NRRL -50104). Bacillus a'uyhhg fa e ABICAS (NRRL B-S0634), Bacillas
Figure imgf000167_0001
&' ( RL B-50508), Baciilm atuylcilqidfyciens 22CP1 (ATCC PTAAAOST BacUlus amyioiiuui cie SS 18 CNRR5 B-50633), BacUlus li henif rmh BAAI2 (NRRL B-S0516), Baciilm Uehemformis BL2I (NRRL, 6-50134), Baaiil subilUs JBPS (NRRL B-S05 K¾ and Bacaibaalli pafymy a ABPI66 (NRRL B-50211) and combinations thereof.
4. 1 bo composition of Claim I, wherein the two strains are Bacillus amyioiiqut/acicas 22CP3 and Bacillus atnylonqtdfaclem 13AP4.
5. The composition or Claim I, wherein the strains are Ac .·'/ /<·.·· ylolhitB/u ie 22CP I and Bacillus my!oUqtii/iic i B 18.
6. The composition of Claim , heroin the strains are Bacillus amy iiquifaaenx BS i 8 and Bacillus amykBiquifaacus \ SAP . 7 The composition of any one of Claim 1 -6, wherein the composition further com ri e ; one or more agtochei ideally active compound's s lect d from the group consisting of: ;· ;; Is - e a r. so- a fungicide, a bactericide, and a nenraticide
8. The composition of Clairo 7, wherein the composition further comprises a compound selected fr m the r u consisting of a safeter, a I ipo-ch itooi i gosaeohitride, an tsofiavone, and a ryanodine receptor modulate?.
9. The composition of Claim 7, wherein the sgroehemieaily active compound is rynaxypyr.
10. The composition of l im '■, wherein the ago >chem leal ly active compound Is cya ypyr.
12, The composition of Claim 7. further comprising at least one genetkally modified seed, plant or plant part.
13, The composition of Claim 7, further comprising at least one seed, plant or plant pari.
1 . The composition of Claim I , wherein the lant pathogen is a bacteria, a fttngus, a nematode.
1 5. The composition of Claim I , wherein the im ov ed plant performance is selected from the group consisting of: increased roof formation, increased root mass, increased toot function, increased shoot height, increased shoot function, increased flo er bud presence, increased flower bud tbmtabom increased total plant wei weight, and increased total plant dry weight, t o, A method comprising applying a composition omprising at least wo bacterial o> :um selected from the group consistmg of Bacillus amyioliquif ck-r . Ik/cUl He ken; form , Bociihv subkks. ami F' rkb cniu pofy yxa to a seed, a. plant, plant part or soil in an effecti ve amount to achieve an effect selected from the group consisting of; inhibit a plant pathogen, improve plant performance, improve plant yield, Improve plant vigor, increase phosphate availability, increase production of a plant hormone, increase soot formation, increase shoot height in a plant increase Seat length of a plant,, i crea e (l wer bud formation of a plant, increase total plan! fresh weight, increase total plant dry weight, and increase seed germination. 1 ?. The meihod of Ciaias iC>, wherein the composition further com ses ¾ biocontrol agen whereas the biocoaao! agent selected from the group conssting of bseaeria, ibrrgi, yeast, protozoas, vinsse , eniomopathogmbe nematodes botanical extracts, prote s, secondary metabolites, and innocuiants.
18. The method of Claim i 6, wherem at leas* iwo strains are selected hom th group conss n of; Baciifus { yioiiqnifci > BS2? (NRRL B iS Baci
Figure imgf000169_0001
BS2084 t RRL B-5 I ). fef& oaa-aLya¾. 15AP4 (A'iCC PTA-65 "?), LCoBar- tm lo!iMxfiXkns 3AP4 (ATCC PTA- 6506), Bash'lm amyb!Ujiiifjcietis LSSAOi (N RL B CH04), Bad! fas iy liquij k ABP278 (NRRL APo.oh Becittu amy tiquifavigm 1013 (NRRL B-S0S09), Bacillus amy Uq ifacism 9) yNRRL B~30S08i, Bacillus i y l nuf em 22CH (ATCC PTA~650¾ BaciU ? o;.o aAeaaoe.. e< BS18 (NRRl B-50633)...b Bfw Ifchemformis UA 2 (NRRL B-5G516}, /3Va;4//av ,7< 0,e;A-aas BL2i (NRRL B-50D4 .Ba I sudtWs 3 EPS (NRRL B-AOS!Oy and Pi?em/wcb¾i■ poiymyxa ABRleC (NRRL B-50211) an combinations thereof
19. lite method of Claim i 6, whereia the two straaes are Saclllm a y iiquifa ie 22CP1 arid Bacill- vi ioUqiiif cifffis iSAP4,
29. Th method of Claim B>< whereia the two embes are NA: .·,'.·<-> rtt k&gmfix kr/is 22CF!atid Bo-'L/e- oaa a - ;ay ;·.·/,··: a BSSR
2 i . The method of Claim 16; wherein the two strains are B eUfas myio!iqt fa s BS 18 sad Ba iih®
Figure imgf000169_0002
f 5AF4,
22, The method oi any one of Claims 16- 1. ohcreht the composition further comprises one or mere aarocheaaea!ly active compounds selected froos the groap consisting of an avv.. io i-.ic. ;s rangioids.. ;s bactericide, and a nematicide.
23, The method of Claim 22, whereia the composition Bather com ises a eompewd sefecie from the group consisting of a selenea a Hpo-cfuiooligosseehafsde, an isofiavone, and a ryanodine receptor modulator.
24. The method of Claira 22, wheein the agrochemicaUy active com und is r naxypyr.
25. The meihod oi" Cham 22, wherein the agroeheafcally active compound is eymypyr. 2 , The method of Claim 16, wherein the plant padiogen is a bacteria, a fungus, a nematode.
27. I He method of Claim 16, wherein the improved plant perforouwscc is electe from the group consi ting of increased root fonnauon. increased rx-a mass, increased root function, increased shoot height increased shoot function, increased llower bud presence, increased flower bod formation, increased total plant wet weight, aitd increased total plant dry weight.
28. An isolated
Figure imgf000170_0001
strain for improving plant go >'■··■■>:■■. plant vigor, or giant yield, w herein the Ba illus strain comprises the BdhA gene, and producer- Indolefo-acetie acid,
29. An isolated Bacillus stein for improving plam performance, plant vigor, or plant yield, whereas ■■):■■: Bacillus strain comprises the trpAB gene, and produces indoie~3~acetic acid.
30. A n isolated B cOhx strain for improving l in performance, plant v igor, or plant yield svherein the &« ½ strain comprises the p P gene, and has the ability to soiubi!hre phosphate.
3 1. An isolated Bacillus strain for improving plant psrforroancs, plant vigor, or plant yield wh rein the Bacillus strain comprises the phoP„ BdhA, and frpAB genes.
32. An i lated Bacillus strain for improving plant perfoommee, plant \ icer. or plant yield wherein the BiKiUm strain produces mdo!e-3-aoctie- acid and has the ability to sokanhze phosphate.
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