Jan Detmers’ Post

𝐒𝐢𝐧𝐠𝐥𝐞-𝐂𝐞𝐥𝐥 𝐌𝐞𝐭𝐚𝐛𝐨𝐥𝐢𝐬𝐦 𝐆𝐫𝐚𝐧𝐭 𝐀𝐰𝐚𝐫𝐝𝐞𝐝 𝐭𝐨 𝐃𝐫. 𝐀𝐤𝐢 𝐌𝐢𝐧𝐨𝐝𝐚 We are pleased to announce that Dr. Aki Minoda, from the Radboud Institute for Molecular Life Sciences in Nijmegen, Netherlands, has been selected as the recipient of the Single-Cell Metabolism Grant. Dr. Minoda's research focuses on the microbiome's impact on cellular aging. Utilizing her expertise, she has developed a comprehensive Mouse Ageing Atlas, integrating transcriptomic and epigenomic single-cell data. Her investigation aims to elucidate the mechanisms underlying chromatin degradation and gene dysregulation during aging and its implications for disease pathogenesis. As part of the grant, Dr. Minoda and her team will benefit from access to QSM™-enabled single-cell metabolic phenotyping capabilities. The outcomes of their research will be disseminated through publications, and the single-cell metabolic data generated using scQSM™ will be accessible to the scientific community. scQSM™ stands as the inaugural technology platform for phenotyping metabolic pathway activities, encompassing bioenergy metabolism (OxPhos) at the single-cell level. It has been validated for immune cells and brain tissue, offering profound insights for both discovery and translational science.   #congratulations #celltherapy #qsm #quantitativesystemmetabolism #doppelganger #cytometry #singlecell #metabolomics #radboud

𝐈𝐦𝐦𝐮𝐧𝐞 𝐂𝐞𝐥𝐥 𝐌𝐞𝐭𝐚𝐛𝐨𝐥𝐢𝐬𝐦: 𝐎𝐩𝐭𝐢𝐦𝐢𝐳𝐢𝐧𝐠 𝐀𝐝𝐨𝐩𝐭𝐢𝐯𝐞 𝐂𝐞𝐥𝐥 𝐓𝐡𝐞𝐫𝐚𝐩𝐢𝐞𝐬 A novel technology enables the quantification of crucial central metabolism pathways, offering fresh avenues for refining Adoptive Cell Therapies. I'm pleased to present a case study illustrating the optimization of FDA-approved CD28 CAR. Our team utilized QSM™ to measure 15 metabolic and 19 proteomic biomarkers in both standard and enhanced CAR-T cells. Our findings highlight the cellular in-vivo ATP production capacity in CAR-T cells as a robust biomarker for treatment efficacy in PDX models. Check out my compelling case study below. #celltherapy #stemcells #qsm #quantitativesystemmetabolism #doppelganger

𝐌𝐞𝐭𝐚𝐛𝐨𝐥𝐢𝐜 𝐞𝐟𝐟𝐞𝐜𝐭𝐬 𝐨𝐟 𝐒𝐚𝐧𝐨𝐟𝐢’𝐬 𝐋𝐢𝐱𝐢𝐬𝐞𝐧𝐚𝐭𝐢𝐝𝐞 𝐨𝐧 𝐑𝐞𝐭𝐢𝐧𝐚 Delighted to share the successful completion of our extensive multiomic analysis, encompassing deep transcriptomic and metabolic profiling of mouse retina samples. Our investigation concentrated on the metabolic impact of Sanofi's Lixisenatide in the context of diabetic retinopathy. This study involved a reanalysis of recently published data by Kuebra Oeser, Matthias Kolibabka, Johann Gassenhuber, Nadine Dietrich, Thomas Fleming, Andrea Schlotterer, Michael Morcos, Paulus Wohlfart, and Hans-Peter Hammes. Our findings reveal that Lixisenatide demonstrates a protective influence on the diabetic retina, underscoring its potential anti-inflammatory and antioxidative effects on the neurovascular unit. Notably, we observed that Lixisenatide did not significantly alter central metabolic pathways. Should you be interested in delving deeper into the comprehensive analysis report, please don't hesitate to get in touch with me via direct message or leave a comment below. Follow us as we look forward to sharing further insights with you! #Research #DrugDevelopment #Sanofi #Lixisenatide #DiabeticRetinopathy #MetabolicAnalysis #GLP1RA #DiabetesResearch #doppelganger #quantitativesystemmetabolism

#Immunology - I am pleased that my team can now quantify immune cell metabolism biomarkers in vivo. The first study by #chineseacademyofsciences shows that CAR-T cells have a 49% reduced ATP production capacity in vivo! In addition, intratumoral CAR-T cells have significantly altered glucose metabolism and show a shift in substrate utilization. Finally, we identified over 50 significantly regulated functional protein markers. Amazing! 🚀 Download the sample analysis report and view all 43 parameters we quantified. 💡   #immunooncology #immunotherapy #celltherapy #targetedtherapy #Tcells #chineseacademyofsciences

𝐈𝐦𝐦𝐮𝐧𝐞 𝐂𝐞𝐥𝐥 𝐌𝐞𝐭𝐚𝐛𝐨𝐥𝐢𝐬𝐦 Researchers at Memorial Sloan Kettering Cancer Center are reporting about ways to improve the performance of CAR-T cells. My team is using QSM™ metabolic profiling of Doppelganger to quantify 43 functional metabolic parameters in vivo. Interestingly, we find that CAR-T constructs differ significantly in glucose metabolism and ATP production capacity. Quantitative analysis of the energy metabolism of immune cells is crucial because immune cells engage in an energy-consuming battle against pathogens, cancer cells, or virus-infected host cells. Therefore, efficient and fast metabolism is crucial for immune functions. Download the full analysis report here: #immunooncology #immunotherapy #celltherapy #targetedtherapy #Tcells

𝐒𝐢𝐧𝐠𝐥𝐞-𝐂𝐞𝐥𝐥 𝐚𝐧𝐝 𝐒𝐩𝐚𝐭𝐢𝐚𝐥 𝐌𝐮𝐥𝐭𝐢-𝐎𝐦𝐢𝐜𝐬 Hot off the press in Nature Reviews Genetics: The integration of genomics, epigenomics, transcriptomics, proteomics, and metabolomics at the single-cell level is revolutionizing our comprehension of cell biology. Over the past decade, groundbreaking technological advancements have facilitated crucial discoveries in understanding the intricate interplay between intracellular and intercellular molecular mechanisms governing development, physiology, and pathogenesis. In a recent review, Katy Vandereyken, Alejandro Sifrim, Bernard Thienpont, and Thierry Voet at the KU Leuven Institute for Single Cell Omics have provided a comprehensive overview of the rapidly evolving field of single-cell and spatial multi-omics technologies, also known as multimodal omics approaches. They also discuss the computational strategies necessary for integrating information across these molecular layers. By highlighting the advancements in single-cell and spatial multi-omics, the authors demonstrate the profound impact of these approaches on fundamental cell biology and translational research. Moreover, they address the current challenges faced in this field and provide an optimistic outlook for the future. This impressive overview underscores the transformative potential of single-cell and spatial multi-omics in unraveling the complexities of cellular processes. It emphasizes the importance of continued innovation and collaboration in propelling the field forward. Check out the original work: Vandereyken, K., Sifrim, A., Thienpont, B. et al. Methods and applications for single-cell and spatial multi-omics. Nat Rev Genet (2023). #SingleCellOmics #SpatialMultiOmics #Genomics #Transcriptomics #Proteomics #Metabolomics #CellBiology #ResearchAdvancements #ScientificReview

𝐍𝐞𝐰 𝐓𝐨𝐨𝐥: 𝐈𝐧𝐯𝐞𝐬𝐭𝐢𝐠𝐚𝐭𝐢𝐧𝐠 𝐏𝐫𝐨𝐦𝐞𝐭𝐚𝐬𝐭𝐚𝐭𝐢𝐜 𝐄𝐟𝐟𝐞𝐜𝐭𝐬 𝐢𝐧 𝐂𝐚𝐧𝐜𝐞𝐫 Fatty acid uptake and altered metabolism are critical features of metastasis, but understanding their role and the specific impact of dietary fatty acids remains incomplete. Miguel Martin-Perez from IRB in Barcelona utilized Doppelganger's QSM platform to explore the influence of fatty acids on cancer cell metastasis. In a recent interview, he shared insights on the effects of different fatty acids in oral cancer. Miguel Martin-Perez's pioneering study revealed the intricate relationship between fatty acids and metastasis. Through meticulous analysis, he demonstrated that specific dietary fatty acids possess prometastatic properties, challenging previous assumptions. Unraveling the underlying molecular mechanisms opens new avenues for therapeutic interventions in metastatic cancer. This interdisciplinary research showcases the power of cutting-edge technology and collaboration in advancing oncology. It represents a significant contribution to the field, with implications extending beyond cancer treatment. #Oncology #CancerResearch #Metastasis #FattyAcids #ResearchInnovation #ScientificBreakthrough #quantitativesystemmetabolism #doppelganger

🔬 Unlocking the Potential of Omics Technologies in CAR T Cell Therapy 🌟 Omics technologies, such as genomics, epigenomics, transcriptomics, T cell receptor-repertoire profiling, proteomics, metabolomics, and microbiomics, have revolutionized CAR T cell therapy. In a recent article Jingwen Yang and her team at Texas A&M University, Houston, Texas, highlight the significance of these multidimensional profiling technologies in CAR T cell research. By leveraging omics technologies, we can gain invaluable insights into CAR T cell therapy. They hold the potential to identify tumor-specific antigens, enabling targeted treatments for cancer patients. Furthermore, these profiling technologies shed light on the molecular characteristics associated with anti-tumor effects and toxicities, leading to enhanced therapy strategies. Jingwen's overview demonstrates the immense value of omics technologies in advancing CAR T cell therapy. Embracing these cutting-edge tools paves the way for personalized medicine and optimized treatments. Together, let's celebrate the power of omics technologies in revolutionizing cancer research and improving patient outcomes. Check out the original open-access article: Yang J, Chen Y, Han L.A multi-omics perspective of CAR T cell therapy. Clin Transl Med.2023;13:e1274. #CellTherapy #OmicsTechnologies #PrecisionMedicine #CancerResearch #CuttingEdgeScience

🔬 Cardiology: Discover new, functional biomarkers! 🔍 🩺 Are you passionate about the latest developments in cardiology and intrigued by the potential of functional biomarkers? If so, this update is tailored for you! 📢 Join me in an insightful interview as I share groundbreaking research conducted by Fábio Trindade from Universidade do Porto in the field of biomarker identification. Fábio's recent work focused on a cohort of patients with aortic stenosis undergoing valve replacement, employing the state-of-the-art Doppelganger's QSM platform. 🔬 Delving into the intricacies of biomarkers, Fábio and his team made significant discoveries that hold profound implications for improving cardiac function through the stimulation of myocardial reverse remodeling. 💡 These findings are not only groundbreaking but also have the potential to reshape the landscape of cardiology. 💪 By identifying these novel biomarkers, we can now pave the way for enhanced cardiac performance, positively impacting patient outcomes and quality of life. ⚡️ This research carries immense value for healthcare professionals and researchers alike, offering valuable insights that can guide future advancements in the field of cardiology. 🌍 Stay informed and be at the forefront of cutting-edge cardiology research and multi-omics bioanalysis by following my updates. 🔗 #Cardiology #FunctionalBiomarkers #MedicalResearch #AdvancementsInHealthcare #Biomarkers #RevolutionizingHealthcare #university_of_porto #quantitativesystemmetamolism #doppelganger