Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. UBM Decellularization
2.2. Decellularization Efficiency
2.2.1. dsDNA Quantification
2.2.2. DAPI Staining
2.2.3. Histological Staining
2.2.4. DNA Electrophoresis
2.3. Residual SDS Detection
2.4. Biochemical Characterization
2.5. UBM Hydrogel Preparation
2.6. Turbidimetric Gelation Kinetics
2.7. Rheologic Testing
2.8. Hydrogel Surface Morphology
2.9. Cytocompatibility
2.9.1. In Vitro Cytotoxicity
2.9.2. D Surface Culture
2.9.3. D Cell Encapsulation
2.10. Statistical Analysis
3. Results
3.1. Decellularization Efficiency
3.2. Biochemical Characterization
3.3. Turbidimetric Gelation Kinetics
3.4. Rheology Study
3.5. Hydrogel Surface Morphology
3.6. Cytocompatibility
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kao, C.-Y.; Nguyen, H.-Q.-D.; Weng, Y.-C. Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method. Polymers 2020, 12, 3007. https://doi.org/10.3390/polym12123007
Kao C-Y, Nguyen H-Q-D, Weng Y-C. Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method. Polymers. 2020; 12(12):3007. https://doi.org/10.3390/polym12123007
Chicago/Turabian StyleKao, Chen-Yu, Huynh-Quang-Dieu Nguyen, and Yu-Chuan Weng. 2020. "Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method" Polymers 12, no. 12: 3007. https://doi.org/10.3390/polym12123007