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Printable (PDF) file Table of Contents C. elegans Researcher Directory. Leon Avery, Theresa Stiernagle and Bob Herman Technician/Research Assistant Position Available at Yale University. Frank Slack Post-Doctoral Research Opportunity: Functional Genomics Studies in Aging. Marco Marra Position at Caltech, CA. Paul Sternberg CGC Bibliographic References. Theresa Stiernagle and Bob Herman WormBase Update. WormBase Consortium Gene Knockout Methods Available Online. Sheldon McKay, Mark Edgley, Erin Gilchrist, Steven Jones and Don Moerman Using spe-26 as an Alternative Marker for Rescue by Biolistic Transformation. Harold E. Smith and Sam Ward RNAi Feeding to Produce Males. Darrell J. Killian and E. Jane Albert Hubbard A Skim Milk-Supplemented Axenic Medium to Support Development and Reproduction of C. elegans. 33 32 31 30 28 3 2 2 1 1
Hugh F. LaPenotiere, Deborah Y. French, Maria Szilagyi and Eric D. Clegg A Rapid, PCR-Based Protocol to Create GFP Fusions. Oliver Hobert 34
A Vector for Pan-Neuronal smg-1-Dependent Transgene Expression. Chris Link, Gin Fonte and Andrew Taft Overexpression of UNC-40 in the Touch Neurons Results in Misdirection of the ALM Axon. Naomi Levy-Strumpf and Joseph G. Culotti Identifying Male Fertility Genes by Recombinant Inbred Mapping. Marc Hammarlund and Erik M. Jorgensen Natural Variation in Egg-Laying Behavior. Michael Ailion and James H. Thomas Understanding the Function of egl-38 and lin-48 Using Heat Shock Promoters hsp16-2 and hsp16-41. Ashwin Uttam and Helen Chamberlin Suppressor Screen of egl-38 Egg-Laying Defect to Study the Vulva to Uterus Signalling Pathway. Vandana Rajakumar and Helen M. Chamberlin Comparative Study of the ovo-Related gene lin-48 Provides Evidence for the Evolution of Transcriptional Regulation. Xiaodong Wang and Helen M. Chamberlin qui-1 Has Been Moleculary Identified and Corresponds to Y45F10B.10.
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Massimo A. Hilliard, Ronald H.A. Plasterk and Paolo Bazzicalupo Common Imprinting for Temperature and Odor Memories. J.J. Remy The C. elegans Gastronome: Differential Palates for Mycobacterium smegmatis and Mycobacterium tuberculosis. Ming Chih Tsai, Jonathan Lipton, Scott Emmons and John Chan Arrhythmicity of the Defecation Oscillator in lin-42 Mutants. Fred Kippert and Mark L. Blaxter Towards a Pristionchus Genome Map I - P. pacificus BAC Library Construction and End Sequencing of BAC Clones. Ralf J. Sommer, Taco Jesse, Stephan C. Schuster, Gunther Raddatz, Heike Keller and Christa Lanz 48 46 45 44
Towards a Pristionchus Genome Map II - Microevolutionary Analysis of the Nematode Genus Pristionchus. Jagan Srinivasan, Andre Pires da Silva, Arturo Gutierrez, Min Zheng, Hanh Witte, Isabel Kipping and Ralf J. Sommer Towards a Pristionchus Genome Map III - SNP Searches and Analyses in Pristionchus pacificus.
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Jagan Srinivasan, Waltraud Sinz, Alexandra Brand, Hanh Witte, Gunther Raddatz, N. Ram Kumar, Stephan C. Schuster and Ralf J. Sommer Towards a Pristionchus Genome Map IV - A Genetic Linkage Map of Pristionchus pacificus. Waltraud Sinz, Jagan Srinivasan, Alexandra Brand, Hanh Witte, Isabel Kipping and Ralf J. Sommer Simulation of Bacterial Transport in the Pharynx. Leon Avery Dye Transport and the Bright Screen. Leon Avery and Charles Heise The Map Kinase Phosphatase LIP-1 Is Required for the Meiotic Cell-Cycle Arrest in Developing Oocytes. Alex Hajnal, Erika Frohli Hoier and Thomas Berset EGL-15 Promotes Protein Degradation in Muscle by Activating the Ras-MAPK Pathway. Nate Szewczyk and Lew Jacobson. Isolation and Characterization of Suppressors of the Ryanodine Receptor Gene unc-68 Mutants. Ryota Adachi and Hiroaki Kagawa Sodium-Dependent Neurotransmitter (snf) Genes in C. elegans. 59 58 56 55 54 52 51
Gregory Mullen, Gary Moulder, Robert Barstead and James Rand The Serotonin-Synthetic AAADC bas-1 Gene Is C05D2.4. E. Hare and C. Loer Cloning of the ceh-13/labial/hox1 Ortholog from C. remanei. 62 60
Francois Gautron, Jose Fos, Charles Stoyanov, Adrian Streit and Fritz Muller
ceh-44, a Cut-Like Gene in C. elegans. N. Pujol, C. Zimmer, T. Burglin and H. Cremer An Attempt to Slow Aging in C. elegans. 20. A Positive Effect of Ascorbic Acid. Vladimir V. Bakaev An Attempt to Slow Aging in C. elegans. 21. No Positive Effect of Vitamin B12. Vladimir V. Bakaev An Attempt to Slow Aging in C. elegans. 22. No Positive Effect of Ascorbic Acid in Postreproductive Period. Vladimir V. Bakaev An Attempt to Slow Aging in C. elegans. 23. No Positive Effect of Ascorbic Acid in Reproductive Period. Vladimir V. Bakaev Mitochondrial Metabolism in C. elegans. Marge Sedensky and Phil Morgan Author Index. Theresa Stiernagle and Bob Herman
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of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390-9148 2 University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Avenue, St. Paul, MN 55108-1095
Please remember to add or confirm your information in the C. elegans Researcher Directory. Even if your information has not changed you still need to confirm your listing. All listings which are not confirmed before March 2002 will be deleted in order to remove those people who are no longer in the C. elegans field. Its a quick and easy process. Just go to http://elegans.swmed.edu/CeRD and find your listing. Make changes, if necessary, and then click "Submit Changes". This will cause an email message to be sent to you. (This step prevents others from changing your information without your knowledge.) Click on the link contained in the email message. Just by following this link you are confirming the information; no need to do anything else unless you want to make changes. Thats it!
Position at Caltech, CA
Paul Sternberg Division of Biology, 156-29, California Institute of Technology, Pasadena, CA, 91125 CURATOR. Will annotate gene functions in C. elegans, using Gene Ontology (Nature Genetics [2000], vol. 25, pp. 25-29). Duties include: analysing gene functions in the primary literature; judging the optimal description of these functions in Gene Ontology, inventing new terms for Gene Ontology where necessary; and incorporating these descriptions into Wormbase. A Ph.D. in some area of biology and substantial C. elegans experience are required. The successful job candidate will have broad scientific erudition, verbal articulacy, and creative intelligence as well as patience and a willingness to work hard. Computer literacy in UNIX or Linux is a plus, but is not required. Direct inquiries to Paul Sternberg (pws@its.caltech.edu).
CGC #4490 Clifford R;Lee MH;Nayak S;Ohmachi M;Giorgini F;Schedl T FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline. Development 127: 5265-5276 2000 ARTICLE CGC #4491 von Zelewsky T;Palladino F;Brunschwig K;Tobler H;Hajnal A;Muller F The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate determination. Development 127: 5277-5284 2000 ARTICLE CGC #4492 Portman DS;Emmons SW The basic helix-loop-helix transcription factors LIN-32 and HLH-2 function together in multiple steps of a C. elegans neuronal sublineage. Development 127: 5415-5426 2000 ARTICLE CGC #4493 Simske JS;Hardin J Getting into shape: epidermal morphogenesis in Caenorhabditis elegans embyros. BioEssays 23: 12-23 2001 REVIEW CGC #4494 Stein L;Sternberg P;Durbin R;Thierry-Mieg J;Spieth J WormBase: network access to the genome and biology of Caenorhabditis elegans. Nucleic Acids Research 29: 82-86 2001 ARTICLE CGC #4495 Signor D;Rose LS;Scholey JM Analysis of the roles of kinesin and dynein motors in microtubule-based transport in the Caenorhabditis elegans nervous system. Methods 22: 317-325 2000 ARTICLE CGC #4496 Arata Y;Hirabayashi J;Kasai KI Application of reinforced frontal affinity chromatography and advanced processing procedure to the study of the binding property of a Caenorhabditis elegans galectin. Journal of Chromatography 905: 337-343 2001 ARTICLE CGC #4497 Reinhart BJ;Ruvkun G Isoform-specific mutations in the Caenorhabditis elegans heterochronic gene lin-14 affect stage-specific patterning.
Genetics 157: 199-209 2001 ARTICLE CGC #4498 Chou JH;Bargmann CI;Sengupta P The Caenorhabditis elegans odr-2 gene encodes a novel Ly-6-related protein required for olfaction. Genetics 157: 211-224 2001 ARTICLE CGC #4499 Edgar LG;Carr S;Wang H;Wood WB Zygotic expression of the caudal homolog pal-1 is required for posterior patterning in Caenorhabditis elegans embryogenesis. Developmental Biology 229: 71-88 2001 ARTICLE CGC #4500 Arata Y;Sekiguchi M;Hirabayashi J;Kasai K Effects of the substitution of conserved amino acid residues on the sugar-binding property of the tandem-repeat 32-kDa galectin of the nematode Caenorhabditis elegans. Biological &; Pharmaceutical Bulletin 24: 14-18 2001 ARTICLE CGC #4501 Gamulin V;Muller IM;Muller WEG Sponge proteins are more similar to those of Homo sapiens than to Caenorhabditis elegans. Biological Journal of the Linnean Society 71: 821-828 2001 ARTICLE CGC #4502 Wilkie TM G-protein signaling: Satisfying the basic necessities of life. Current Biology 10: R853-R856 2000 REVIEW CGC #4503 Zhou Z;Hartwieg E;Horvitz HR CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans. Cell 104: 43-56 2001 ARTICLE CGC #4504 Kramerova IA;Kawaguchi N;Fessler LI;Nelson RE;Chen Y;Kramerov AA;Kusche-Gullberg M;Kramer JM;Ackley BD;Sieron AL;Prockop DJ;Fessler JH Papilin in development; a pericellular protein with a homology to the ADAMTS metalloproteinases. Development 127: 5475-5485 2000 ARTICLE
CGC #4505 Tatusov RL;Natale DA;Garkavtsev IV;Tatusova TA;Shankavaram UT;Rao BS;Kiryutin B;Galperin MY;Fedorova ND;Koonin EV The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Research 29: 22-28 2001 ARTICLE CGC #4506 Costanzo MC;Crawford ME;Hirschman JE;Kranz JE;Olsen P;Robertson LS;Skrzypek MS;Braun BR;Hopkins KL;Kondu P;Lengieza C;Lew-Smith JE;Tillberg M;Garrels JI YPD, PombePD and WormPD: model organism volumes of the BioKnowledge Library, an integrated resource for protein information. Nucleic Acids Research 29: 75-79 2001 ARTICLE CGC #4507 Hsieh J;Fire A Recognition and silencing of repeated DNA. Annual Review of Genetics 34: 187-204 2000 REVIEW CGC #4508 Hill E;Broadbent ID;Chothia C;Pettitt J Cadherin superfamily proteins in Caenorhabditis elegans and Drosophila melanogaster. Journal of Molecular Biology 305: 1011-1024 2001 ARTICLE CGC #4509 Kamath RS;Martinez-Campos M;Zipperlen P;Fraser AG;Ahringer J Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biology 2: 1-10 2000 ARTICLE CGC #4510 Bossinger O;Klebes A;Segbert C;Theres C;Knust E Zonula adherens formation in Caenorhabditis elegans requires dlg-1, the homologue of the Drosophila gene discs large. Developmental Biology 230: 29-42 2001 ARTICLE CGC #4511 Jonassen T;Larsen PL;Clarke CF A dietary source of coenzyme Q is essential for growth of long-lived Caenorhabditis elegans clk-1 mutants. Proceedings of the National Academy of Sciences USA 98: 421-426 2001 ARTICLE
CGC #4512 Hart AH;Reventar R;Bernstein A Genetic analysis of ETS genes in C. elegans. Oncogene 19: 6400-6408 2000 ARTICLE CGC #4513 Grill SW;Gonczy P;Stelzer EHK;Hyman AA Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo. Nature 409: 630-633 2001 ARTICLE CGC #4514 Xiao GQ;Wang J;Tangen T;Giacomini KM A novel proton-dependent nucleoside transporter, CeCNT3, from Caenorhabditis elegans. Molecular Pharmacology 59: 339-348 2001 ARTICLE CGC #4515 Fitch DHA Evolution of "Rhabditidae" and the male tail. Journal of Nematology 32: 235-244 2000 ARTICLE CGC #4516 Gems D An integrated theory of ageing in the nematode Caenorhabditis elegans. Journal of Anatomy 197: 521-528 2000 ARTICLE CGC #4517 Yoda A;Sawa H;Okano H MSI-1, a neural RNA-binding protein, is involved in male mating behaviour in Caenorhabditis elegans. Genes to Cells 5: 885-895 2000 ARTICLE CGC #4518 Cai T;Krause MW;Odenwald WF;Toyama R;Notkins AL The IA-2 gene family: homologs in Caenorhabditis elegans, Drosophila and zebrafish. Diabetologia 44: 81-88 2001 ARTICLE CGC #4519 Doe CQ;Bowerman B Asymmetric cell division: fly neuroblast meets worm zygote. Current Opinion in Cell Biology 13: 68-75 2001 REVIEW
CGC #4520 Rudel D;Kimble J Conservation of glp-1 regulation and function in nematodes. Genetics 157: 639-654 2001 ARTICLE CGC #4521 Miskowski J;Li Y;Kimble J The sys-1 gene and sexual dimorphism during gonadogenesis in Caenorhabditis elegans. Developmental Biology 230: 61-73 2001 ARTICLE CGC #4522 Siklos S;Jasper JA;Wicks SR;Rankin CH Interactions between an endogenous oscillator and response to tap in C. elegans. Psychobiology 28: 571-580 2000 ARTICLE CGC #4523 Crump JG;Zhen M;Jin Y;Bargmann CI The SAD-1 kinase regulates presynaptic vesicle clustering and axon termination. Neuron 29: 115-129 2001 ARTICLE CGC #4524 Huang T;Kuersten S;Deshpande AM;Spieth J;MacMorris M;Blumenthal T Intercistronic region required for polycistronic pre-mRNA processing in Caenorhabditis elegans. Molecular &; Cellular Biology 21: 1111-1120 2001 ARTICLE CGC #4525 Oishi I;Iwai K;Kagohashi Y;Fujimoto H;Kariya K;Kataoka T;Sawa H;Okano H;Otani H;Yamamura H;Minami Y Critical role of Caenorhabditis elegans homologs of Cds1 (Chk2)-related kinases in meiotic recombination. Molecular &; Cellular Biology 21: 1329-1335 2001 ARTICLE CGC #4526 Thatcher JD;Fernandez AP;Beaster-Jones L;Haun C;Okkema PG The Caenorhabditis elegans peb-1 gene encodes a novel DNA-binding protein involved in morphogenesis of the pharynx, vulva, and hindgut. Developmental Biology 229: 480-483 2001 ARTICLE CGC #4527 Jin SW;Kimble J;Ellis RE Regulation of cell fate in Caenorhabditis elegans by a novel cytoplasmic polyadenylation element binding protein.
Developmental Biology 229: 537-553 2001 ARTICLE CGC #4528 Gems D Longevity and ageing in parasitic and free-living nematodes. Biogerontology 1: 289-307 2001 REVIEW CGC #4529 Sprengel R;Aronoff R;Volkner M;Schmitt B;Mosbach R;Kuner T Glutamate receptor channel signatures. Trends in Pharmacological Sciences 22: 7-10 2001 REVIEW CGC #4530 Yu L;Gaitatzes C;Neer E;Smith TF Thirty-plus functional families from a single motif. Protein Science 9: 2470-2476 2001 ARTICLE CGC #4531 Momin RA;Nair MG Mosquitocidal, nematicidal, and antifungal compounds from Apium graveolens L. seeds. Journal of Agricultural &; Food Science 49: 142-145 2001 ARTICLE CGC #4532 Thomas GH Spectrin: the ghost in the machine. BioEssays 23: 152-160 2001 REVIEW CGC #4533 Hoss S;Bergtold M;Haitzer M;Traunspurger W;Steinberg CE Refractory dissolved organic matter can influence the reproduction of Caenorhabditis elegans (Nematoda). Freshwater Biology 46: 1-10 2001 ARTICLE CGC #4534 Jungblut B;Sommer RJ The nematode even-skipped homolog vab-7 regulates gonad and vulva position in Pristionchus pacificus. Development 128: 253-261 2001 ARTICLE CGC #4535 Jung SK;Ahnn JH Genetic analysis of jh2 mutation near the unc-29 locus of chromosome I in Caenorhabditis elegans. Korean Journal of Genetics 22: 331-340 2000
ARTICLE CGC #4536 Harrison PM;Echols N;Gerstein MB Digging for dead genes: an analysis of the characteristics of the pseudogene population in the Caenorhabditis elegans genome. Nucleic Acids Research 29: 818-830 2001 ARTICLE CGC #4537 Arata Y;Hirabayashi J;Kasai K Sugar-binding properties of the two lectin domains of the tandem repeat-type galectin LEC-1 (N32) of Caenorhabditis elegans. Journal of Biological Chemistry 276: 3068-3077 2001 ARTICLE CGC #4538 Greener T;Grant B;Zhang Y;Wu X;Greene LE;Hirsh D;Eisenberg E Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo. Nature Cell Biology 3: 215-219 2001 ARTICLE CGC #4539 Hodgkin J;Kuwabara PE;Corneliussen B A novel bacterial pathogen, Microbacterium nematophilum, induces mrophological change in the nematode Caenorhabditis elegans. Current Biology 10: 1615-1618 2001 ARTICLE CGC #4540 Piano F;Schetter AJ;Mangone M;Stein L;Kemphues KJ RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans. Current Biology 10: 1619-1622 2001 ARTICLE CGC #4541 Ayyadevara S;Ayyadevara R;Hou S;Thaden JJ;Reis RJS Genetic mapping of quantitative trait loci governing longevity of Caenorhabditis elegans in recombinant-inbred progeny of a Bergerac BO X RC301 interstrain cross. Genetics 157: 655-666 2001 ARTICLE CGC #4542 Berset T;Hoier EF;Battu G;Canevascini S;Hajnal A Notch inhibition of Ras signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development. Science 291: 1055-1058 2001 ARTICLE
CGC #4543 Momin RA;Ramsewak RS;Nair MG Bioactive compounds and 1,3-Di[(cis)-9-octadecenoyl]-2-[(cis,cis)-9,12-octadecadienoyl]glycerol from Apium Graveolens L. seeds. Journal of Agricultural and Food Chemistry 48: 3785-3788 2000 ARTICLE CGC #4544 Severson AF;Hamill DR;Carter JC;Schumacher J;Bowerman B The Aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis. Current Biology 10: 1162-1171 2000 ARTICLE CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 ARTICLE CGC #4546 Liu Y;Huang T;MacMorris M;Blumenthal T Interplay between AAUAAA and the trans-splice site in processing of a Caenorhabditis elegans operon pre-mRNA. RNA 7: 176-181 2001 ARTICLE CGC #4547 Bianchi L;Miller DM;George AL Expression of a CIC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons. Neuroscience Letters 299: 177-180 2001 ARTICLE CGC #4548 Muller SA;Haner M;Ortiz I;Aebi U;Epstein HF STEM analysis of Caenorhabditis elegans muscle thick filaments: Evidence for microdifferentiated substructures. Journal of Molecular Biology 305: 1035-1044 2001 ARTICLE CGC #4549 Seydoux G;Schedl T The germline in C. elegans: Origins, proliferation, and silencing. International Review of Cytology - A Survey of Cell Biology 203: 139-185 2001 REVIEW GCC #4550 Clements D;Rex M;Woodland HR Initiation and early patterning of the endoderm. International Review of Cytology - A Survey of Cell Biology 203: 383-446 2001 REVIEW
CGC #4551 Cho JH;Bandyopadhyay J;Lee J;Park CS;Ahnn J Two isoforms of sarco/endoplasmic reticulum calcium ATPase (SERCA) are essential in Caenorhabditis elegans. Gene 261: 211-219 2000 ARTICLE CGC #4552 Takemoto T;Sasaki Y;Hamajima N;Goshima Y;Nonaka M;Kimura H Cloning and characterization of the Caenorhabditis elegans CeCRMP/DHP-1 and -2; common ancestors of CRMP and dihydropyrimidinase? Gene 261: 259-267 2000 ARTICLE CGC #4553 Beaudoin F;Michaelson LV;Lewis MJ;Shewry PR;Sayanova O;Napier JA Production of C-20 polyunsaturated fatty acids (PUFAs) by pathway engineering: identification of a PUFA elongase component from Caenorhabditis elegans. Biochemical Society Transactions 28: 661-663 2000 ARTICLE CGC #4554 Bouvier-Nave P;Benveniste P;Noiriel A;Schaller H Expression in yeast of an acyl-CoA:diacylglycerol acyltransferase cDNA from Caenorhabditis elegans. Biochemical Society Transactions 28: 692-695 2000 ARTICLE CGC #4555 Meesapyodsuk D;Reed DW;Savile CK;Buist PH;Schafer UA;Ambrose SJ;Covello PS Substrate specificity, regioselectivity and cryptoregiochemistry of plant and animal omega-3 fatty acid desaturases. Biochemical Society Transactions 28: 632-635 2000 ARTICLE CGC #4556 Das T;Thurmond JM;Bobik E;Leonard AE;Parker-Barnes JM;Huang YS;Mukerji P Polyunsaturated fatty acid-specific elongation enzymes. Biochemical Society Transactions 28: 658-660 2000 ARTICLE CGC #4557 Xie T;Ding D Investigating 42 candidate orthologous protein groups by molecular evolutionary analysis on genome scale. Gene 261: 305-310 2000 ARTICLE CGC #4558 Luzi L;Confalonieri S;Di Fiore PP;Pelicci PG Evolution of Shc functions from nematode to human. Current Opinion in Genetics &; Development 10: 668-674 2000 ARTICLE
CGC #4559 Strange K Model organisms: comparative physiology or just physiology? American Journal of Physiology-Cell Physiology 279: C2050-C2051 2000 REVIEW CGC #4560 Stein L;Mangone M;Schwarz E;Durbin R;Thierry-Mieg J;Spieth J;Sternberg P WormBase: network access to the genome and biology of Caenorhabditis elegans. Nucleic Acids Research 29: 1012 2001 ARTICLE CGC #4561 Redmond DL;Clucas C;Johnstone IL;Knox DP Expression of Haemonchus contortus pepsinogen in Caenorhabditis elegans. Molecular &; Biochemical Parasitology 112: 125-131 2001 ARTICLE CGC #4562 Stansberry J;Baude EJ;Taylor MK;Chen PJ;Jin SW;Ellis RE;Uhler MD A cGMP-dependent protein kinase is implicated in wild-type motility in C. elegans. Journal of Neurochemistry 76: 1177-1187 2001 ARTICLE CGC #4563 Barstead R Genome-wide RNAi. Current Opinion in Chemical Biology 5: 63-66 2001 REVIEW CGC #4564 Hengartner MO Apoptosis: Corralling the corpses. Cell 104: 325-328 2001 REVIEW CGC #4565 Link CD;Johnson CJ;Fonte V;Paupard MC;Hall DH;Styren S;Mathis CA;Klunk WE Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34. Neurobiology of Aging 22: 217-226 2001 ARTICLE CGC #4566 Tissenbaum HA;Guarente L Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature 410: 227-230 2001 ARTICLE
CGC #4567 Brockie PJ;Madsen DM;Zheng Y;Mellem J;Maricq AV Differential expression of glutamate receptor subunits in the nervous system of Caenorhabditis elegans and their regulation by the homeodomain protein UNC-42. Journal of Neuroscience 21: 1510-1522 2001 ARTICLE CGC #4568 Gower NJD;Temple GR;Schein JE;Marra M;Walker DS;Baylis HA Dissection of the promoter region of the inositol 1,4,5-triphospate receptor gene, itr-1, in C. elegans: A molecular basis for cell-specific expression of IP3R isoforms. Journal of Molecular Biology 306: 145-157 2001 ARTICLE CGC #4569 Ono S;McGough A;Pope BJ;Tolbert VT;Bui A;Pohl J;Benian GM;Gernert KM;Weeds AG The C-terminal tail of UNC-60B (actin depolymerizing factor/cofilin) is critical for maintaining its stable association with F-actin and is implicated in the second actin-binding site. Journal of Biological Chemistry 276: 5952-5958 2001 ARTICLE CGC #4570 Ishii N Oxidative stress and aging in Caenorhabditis elegans. Free Radical Research 33: 857-864 2000 ARTICLE CGC #4571 Singson A Every sperm is sacred: Fertilization in Caenorhabditis elegans. Developmental Biology 230: 101-109 2001 REVIEW CGC #4572 Robertson HM Updating the str and srj (stl) families of chemoreceptors in Caenorhabditis nematodes reveals frequent gene movement within and between chromosomes. Chemical Senses 26: 151-159 2001 ARTICLE CGC #4573 Azim MK;Grossmann JG;Zaidi ZH Homology modeling of nematode Caenorhabditis elegans CED3 protein-inhibitor complex. Biochemical &; Biophysical Research Communications 281: 115-121 2001 ARTICLE
CGC #4574 Aballay A;Ausubel FM Programmed cell death mediated by ced-3 and ced-4 protects Caenorhabditis elegans from Salmonella typhimurium-mediated killing. Proceedings of the National Academy of Sciences USA 98: 2735-2739 2001 ARTICLE CGC #4575 Reboul J;Vaglio P;Tzellas N;Thierry-Mieg N;Moore T;Jackson C;Shin-i T;Kohara Y;Thierry-Mieg D;Thierry-Mieg J;Lee H;Hitti J;Doucette-Stamm L;Hartley JL;Temple GF;Brasch MA;Vandenhaute J;Lamesch PE;Hill Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans. Nature Genetics 27: 332-336 2001 ARTICLE CGC #4576 Gotta M;Ahringer J Distinct roles for G alpha and G beta gamma in regulating spindle position and orientation in Caenorhabditis elegans embryos. Nature Cell Biology 3: 297-300 2001 ARTICLE CGC #4577 Hu S;Lee R;Zhang Z;Krylov SN;Dovichi NJ Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis. Journal of Chromatography B 752: 307-310 2001 ARTICLE CGC #4578 Kranewitter WJ;Ylanne J;Gimona M UNC-87 is an actin-bundling protein. Journal of Biological Chemistry 276: 6306-6312 2001 ARTICLE CGC #4579 Herman MA C. elegans POP-1/TCF functions in a canonical Wnt pathway that controls cell migration and in a noncanonical Wnt pathway that controls cell polarity. Development 128: 581-590 2001 ARTICLE CGC #4580 Michalski AI;Johnson ME;Cypser JR;Yashin AI Heating stress patterns in Caenorhabditis elegans longevity and survivorship. Biogerontology 2: 35-44 2001 ARTICLE CGC #4581 Friedman R;Hughes AL Gene duplication and the structure of eukaryotic genomes. Genome Research 11: 373-381 2001
ARTICLE CGC #4582 Boulton SJ;Vincent S;Vidal M Use of protein-interaction maps to formulate biological questions. Current Opinion in Chemical Biology 5: 57-62 2001 REVIEW CGC #4583 Ball CA;Cherry JM Genome comparisons highlight similarity and diversity within the eukaryotic kingdoms. Current Opinion in Chemical Biology 5: 86-89 2001 REVIEW CGC #4584 Turner AJ;Isaac RE;Coates D The neprilysin (NEP) family of zinc metalloendopeptidases: genomics and function. BioEssays 23: 261-269 2001 REVIEW CGC #4585 Anderson GL;Boyd WA;Williams PL Assessment of sublethal endpoints for toxicity testing with the nematode Caenorhabditis elegans. Environmental Toxicology and Chemistry 20: 833-838 2001 ARTICLE CGC #4586 Zarkower D Establishing sexual dimorphism: Conservation amidst diversity? Nature Reviews Genetics 2: 175-185 2001 REVIEW CGC #4587 Sanjuan R;Marin I Tracing the origin of the compensasome: Evolutionary history of DEAH helicase and MYST acetyltransferase gene families. Molecular Biology and Evolution 18: 330-343 2001 ARTICLE CGC #4588 Trachtulec Z;Forejt J Synteny of orthologous genes conserved in mammals, snake, fly, nematode, and fission yeast. Mammalian Genome 12: 227-231 2001 ARTICLE CGC #4589 Aravind L;Dixit VM;Koonin EV Apoptotic molecular machinery: Vastly increased complexity in vertebrates revealed by genome comparisons.
Science 291: 1279-1284 2001 ARTICLE CGC #4590 Dolinski C;Baldwin JG;Thomas WK Comparative survey of early embryogenesis of Secernentea (Nematoda), with phylogenetic implications. Canadian Journal of Zoology 79: 82-94 2001 ARTICLE CGC #4591 Miller MA;Nguyen VQ;Lee MH;Kosinski M;Schedl T;Caprioli RM;Greenstein D A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation. Science 291: 2144-2147 2001 ARTICLE CGC #4592 Hartman PS;Hlavacek A;Wilde H;Lewicki D;Schubert W;Kern RG;Kazarians GA;Benton EV;Benton ER;Nelson GA A comparison of mutations induced by accelerated iron particles versus those induced by low earth orbit space radiation in the FEM-3 gene of Caenorhabditis elegans. Mutation Research 474: 47-55 2001 ARTICLE CGC #4593 Gilleard JS;McGhee JD Activation of hypodermal differentiation in the Caenorhabditis elegans embryo by GATA transcription factors ELT-1 and ELT-3. Molecular &; Cellular Biology 21: 2533-2544 2001 ARTICLE CGC #4594 Lickteig KM;Duerr JS;Frisby DL;Hall DH;Rand JB;Miller DM Regulation of neurotransmitter vesicles by the homeodomain protein UNC-4 and its transcriptional corepressor UNC-37/Groucho in Caenorhabditis elegans cholinergic motor neurons. Journal of Neuroscience 21: 2001-2014 2001 ARTICLE CGC #4595 Miyadera H;Amino H;Hiraishi A;Taka H;Murayama K;Miyoshi H;Sakamoto K;Ishii N;Hekimi S;Kita K Altered quinone biosynthesis in the long-lived clk-1 mutants of Caenorhabditis elegans. Journal of Biological Chemistry 276: 7713-7716 2001 ARTICLE CGC #4596 Chin GM;Villeneuve AM C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G(2) DNA damage checkpoint.
Genes &; Development 15: 522-534 2001 ARTICLE CGC #4597 Timmons L;Court DL;Fire A Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263: 103-112 2001 ARTICLE CGC #4598 Frame IG;Cutfield JF;Poulter RTM New BEL-like LTR-retrotransposons in Fugu rubripes, Caenorhabditis elegans, and Drosophila melanogaster. Gene 263: 219-230 2001 ARTICLE CGC #4599 Barsyte D;Lovejoy DA;Lithgow GJ Longevity and heavy metal resistance in daf-2 and age-1 long-lived mutants of Caenorhabditis elegans. FASEB Journal 15: 627-634 2001 ARTICLE CGC #4600 Villeneuve AM Development - How to stimulate your partner. Science 291: 2209- 2001 REVIEW CGC #4601 Pujol N;Bonnerot C;Ewbank JJ;Kohara Y;Thierry-Mieg D The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ATPase alpha subunit. Journal of Biological Chemistry 276: 11913-11921 2001 ARTICLE CGC #4602 Wang S;Kimble J The TRA-1 transcription factor binds TRA-2 to regulate sexual fates in Caenorhabditis elegans. EMBO Journal 20: 1363-1372 2001 ARTICLE CGC #4603 Haycraft CJ;Swoboda P;Taulman PD;Thomas JH;Yoder BK The C. elegans homolog of the murine cystic kidney disease gene Tg737 functions in a ciliogenic pathway and is disrupted in osm-5 mutant worms. Development 128: 1493-1505 2001 ARTICLE
CGC #4604 Schaefer AM;Nonet ML Cellular and molecular insights into presynaptic assembly. Current Opinion in Neurobiology 11: 137-134 2001 REVIEW CGC #4605 Sharp PA RNA interference - 2001. Genes &; Development 15: 485-190 2001 REVIEW CGC #4606 Chen J;Carey JR;Ferris H Comparative demography of isogenic populations of Caenorhabditis elegans. Experimental Gerontology 36: 431-440 2001 ARTICLE CGC #4607 Horoszok L;Raymond V;Sattelle DB;Wolstenholme AJ GLC-3: a novel fipronil and BIDN-sensitive, but picrotoxinin-insensitive, L-glutamate-gated chloride channel subunit from Caenorhabditis elegans. British Journal of Pharmacology 132: 1247-1254 2001 ARTICLE CGC #4608 Ono S The Caenorhabditis elegans unc-78 gene encodes a homologue of actin-interacting protein 1 required for organized assembly of muscle actin filaments. Journal of Cell Biology 152: 1313-1319 2001 ARTICLE CGC #4609 Pierce-Shimomura JT;Faumont S;Gaston MR;Pearson BJ;Lockery SR The homeobox gene lim-6 is required for dinstinct chemosensory representations in C. elegans. Nature 410: 694-698 2001 ARTICLE CGC #4610 Wes PD;Bargmann CI C. elegans odour discrimination requires asymmetric diversity in olfactory neurons. Nature 410: 698-701 2001 ARTICLE CGC #4611 Pujol N;Ewbank JJ C. elegans: from sequencing to systematic functional genomic. M S-Medecine Sciences 17: 355-357 2001 REVIEW
CGC #4612 Kiehl TR;Shibata H;Pulst SM The ortholog of human ataxin-2 is essential for early embryonic patterning in C. elegans. Journal of Molecular Neuroscience 15: 231-241 2000 ARTICLE CGC #4613 Marais G;Duret L Synonymous codon usage, accuracy of translation, and gene length in Caenorhabditis elegans. Journal of Molecular Evolution 52: 275-280 2001 ARTICLE CGC #4614 Gieseler K;Mariol MC;Bessou C;Migaud M;Franks CJ;Holden-Dye L;Segalat L Molecular, genetic and physiological characterisation of dystrobrevin-like (dyb-1) mutants of Caenorhabditis elegans. Journal of Molecular Biology 307: 107-117 2001 ARTICLE CGC #4615 Ackley BD;Crew JR;Elamaa H;Pihlajaniemi T;Kuo CJ;Kramer JM The NC1/endostatin domain of Caenorhabditis elegans type XVIII collagen affects cell migration and axon guidance. Journal of Cell Biology 152: 1219-1232 2001 ARTICLE CGC #4616 Zhang H;Emmons SW The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene expression. Development 128: 767-777 2001 ARTICLE CGC #4617 Burglin TR;Ruvkun G Regulation of ectodermal and excretory function by the C. elegans POU homeobox gene ceh-6. Development 128: 779-790 2001 ARTICLE CGC #4618 Carthew RW Gene silencing by double-stranded RNA. Current Opinion in Cell Biology 13: 244-248 2001 ARTICLE CGC #4619 Berninsone P;Hwang HY;Zemtseva I;Horvitz HR;Hirschberg CB SQV-7, a protein involved in Caenorhabditis elegans epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-N-acetylgalactosamine, and UDP-galactose.
Proceedings of the National Academy of Sciences USA 98: 3738-3743 2001 ARTICLE CGC #4620 Kang ZB;Ge Y;Chen Z;Cluette-Brown J;Laposata M;Leaf A;Kang JX Adenoviral gene transfer of Caenorhabditis elegans n-3 fatty acid desaturase optimizes fatty acid composition in mammalian cells. Proceedings of the National Academy of Sciences USA 98: 4050-4054 2001 ARTICLE CGC #4621 Yassin L;Gillo B;Kahan T;Halevi S;Eshel M;Treinin M Characterization of the DEG-3/DES-2 receptor: A nicotinic acetylcholine receptor that mutates to cause neuronal degeneration. Molecular &; Cellular Neuroscience 17: 589-599 2001 ARTICLE CGC #4622 Rose JK;Rankin CH Analysis of habituation in Caenorhabditis elegans. Learning &; Memory 8: 63-69 2001 REVIEW CGC #4623 Pierce SB;Costa M;Wisotzkey R;Devadhar S;Homburger SA;Buchman AR;Ferguson KC;Heller J;Platt DM;Pasquinelli AA;Liu LX;Doberstein SK;Ruvkun G Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family. Genes &; Development 15: 672-686 2001 ARTICLE CGC #4624 Wu J;Duggan A;Chalfie M Inhibition of touch cell fate by egl-44 and egl-46 in C. elegans. Genes &; Development 15: 789-802 2001 ARTICLE CGC #4625 Lagido C;Pettitt J;Porter AJR;Paton GI;Glover LA Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors. FEBS Letters 493: 36-39 2001 ARTICLE CGC #4626 Hill KL;LHernault SW Analyses of reproductive interactions that occur after heterospecific matings within the genus Caenorhabditis. Developmental Biology 232: 105-114 2001 ARTICLE
CGC #4627 Vogel BE;Hedgecock EM Hemicentin, a conserved extracellular member of the immunoglobulin superfamily, organizes epithelial and other cell attachments into oriented line-shaped junctions. Development 127: 883-894 2001 ARTICLE CGC #4628 Blomberg N;Baraldi E;Sattler M;Saraste M;Nilges M Structure of a PH domain from the C. elegans muscle protein UNC-89 suggests a novel function. Structure 8: 1079-1087 2001 ARTICLE CGC #4629 Cowen T A heady message for lifespan regulation. Trends in Genetics 17: 109-113 2001 REVIEW CGC #4630 Jedrusik MA;Schulze E A single histone H1 isoform (H1.1) is essential for chromatin silencing and germline development in Caenorhabditis elegans. Development 128: 1069-1080 2001 ARTICLE CGC #4631 Tenenhaus C;Subramaniam K;Dunn MA;Seydoux G PIE-1 is a bifunctional protein that regulates maternal and zygotic gene expression in the embryonic germ line of Caenorhabditis elegans. Genes &; Development 15: 1031-1040 2001 ARTICLE CGC #4632 Rivas E;Eddy SR Secondary structure alone is generally not statistically significant for the detection of noncoding RNAs. Bioinformatics 16: 583-605 2000 ARTICLE CGC #4633 Bargmann C Simple organisms. Neurobiology of Disease 7: 520-522 2000 REVIEW CGC #4634 Page BD;Guedes S;Waring D;Priess JR The C. elegans E2F- and DP-related proteins are required for embyronic asymmetry and negatively regulate Ras/MAPK signaling. Molecular Cell 7: 451-460 2001
ARTICLE CGC #4635 Ceol CJ;Horvitz HR dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in C. elegans vulval development. Molecular Cell 7: 461-473 2001 ARTICLE CGC #4636 Maduro MF;Meneghini MD;Bowerman B;Broitman-Maduro G;Rothman JH Restriction of mesendoderm to a single blastomere by the combined action of SKN-1 and a GSK-3 beta homolog is mediated by MED-1 and -2 in C. elegans. Molecular Cell 7: 475-485 2001 ARTICLE CGC #4637 Kuwabara PE;ONeil N The use of functional genomics in C. elegans for studying human development and disease. Journal of Inherited Metabolic Disease 24: 127-138 2001 REVIEW CGC #4638 Zhang L;Wu SL;Rubin CS A novel adapter protein employs a phosphotyrosine binding domain and exceptionally basic N-terminal domains to capture and localize an atypicl protein kinase C. Journal of Biological Chemistry 276: 10463-10475 2001 ARTICLE CGC #4639 Zhang L;Wu SL;Rubin CS Structural properties and mechanisms that govern association of C kinase adapter 1 with protein kinase C3 and the cell periphery. Journal of Biological Chemistry 276: 10476-10484 2001 ARTICLE CGC #4640 Wang MQ;Sternberg PW Pattern formation during C. elegans vulval induction. Current Topics in Developmental Biology 51: 189-220 2001 REVIEW CGC #4641 Sok J;Calfon M;Lu J;Lichtlen P;Clark SG;Ron D Arsenite-inducible RNA-associated protein (AIRAP) protects cells from arsenite toxicity. Cell Stress &; Chaperones 6: 6-15 2001 ARTICLE
CGC #4642 Gottlieb RA Mitochondria: execution central. FEBS Letters 482: 6-12 2000 REVIEW CGC #4643 Selleck SB Genetic dissection of proteoglycan function in Drosophila and C. elegans. Seminars in Cell &; Developmental Biology 12: 127-134 2001 REVIEW CGC #4644 Salkoff L;Butler A;Fawcett G;Kunkel M;McArdle C;Paz-y-Mino G;Nonet M;Walton N;Wang ZW;Yuan A;Wei A Evolution tunes the excitability of individual neurons. Neuroscience 103: 853-859 2001 ARTICLE CGC #4645 Pemberton DJ;Franks CJ;Walker RJ;Holden-Dye L Characterization of glutamate-gated chloride channels in the pharynx of wild-type and mutant Caenorhabditis elegans delineates the role of the subunit GluCl-a2 in the function of the native receptor. Molecular Pharmacology 59: 1037-1043 2001 ARTICLE CGC #4646 OLone RB;Campbell WC Effect of refrigeration on the antinematodal efficacy of ivermectin. Journal of Parasitology 87: 452-454 2001 ARTICLE CGC #4647 West RJ;Sun AY;Church DL;Lambie EJ The C. elegans gon-2 gene encodes a putative TRP cation channel protein required for mitotic cell cycle progression. Gene 266: 103-110 2001 ARTICLE CGC #4648 Campbell AM;Teesdale-Spittle PH;Barrett J;Liebau E;Jefferies JR;Brophy PM A common class of nematode glutathione S-transferase (GST) revealed by the theoretical proteome of the model organism Caenorhabditis elegans. Comparative Biochemistry &; Physiology B-Biochemistry &; Molecular Biology 128: 701-708 2001 ARTICLE CGC #4649 Sedensky MM;Siefker JM;Morgan PG Model organisms: New insights into ion channel and transporter function. Stomatin homologues interact in Caenorhabditis elegans. American Journal of Physiology - Cell Physiology 280: C1340-C1348 2001
ARTICLE CGC #4650 Rutledge E;Bianchi L;Christensen M;Boehmer C;Morrison R;Broslat A;Beld AM;Geroge AL;Greenstein D;Strange K CLH-3, a ClC-2 anion channel ortholog activated during meiotic maturation in C. elegans oocytes. Current Biology 11: 161-170 2001 ARTICLE CGC #4651 Maeda I;Kohara Y;Yamamoto M;Sugimoto A Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Current Biology 11: 171-176 2001 ARTICLE CGC #4652 Kim SK Functional genomics: The worm scores a knockout. Current Biology 11: R85-R87 2001 REVIEW CGC #4653 Nass R;Miller DM;Blakely RD C. elegans: a novel pharmacogenetic model to study Parkinsons disease. Parkinsonism &; Related Disorders 7: 185-191 2001 ARTICLE CGC #4654 Kay AJ;Hunter CP CDC-42 regulates PAR protein localization and function to control cellular and embyronic polarity in C. elegans. Current Biology 11: 474-481 2001 ARTICLE CGC #4655 Fares H;Greenwald I Regulation of endocytosis by CUP-5, the Caenorhabditis elegans mucolipin-1 homolog. Nature Genetics 28: 64-68 2001 ARTICLE CGC #4656 Cahill CM;Tzivion G;Nasrin N;Ogg S;Dore J;Ruvkun G;Alexander-Bridges M Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways. Journal of Biological Chemistry 276: 13402-13410 2001 ARTICLE
CGC #4657 Xu L;Fong Y;Strome S The Caenorhabditis elegans maternal-effect sterile proteins, MES-2, MES-3, and MES-6, are associated in a complex in embryos. Proceedings of the National Academy of Sciences USA 98: 5061-5066 2001 ARTICLE CGC #4658 Joshua GWP Functional analysis of leucine aminopeptidase in Caenorhabditis elegans. Molecular &; Biochemical Parasitology 113: 223-232 2001 ARTICLE CGC #4659 Van Baelen K;Vanoevelen J;Missiaen L;Raeymaekers L;Wuytack F The golgi PMR1 P-type ATPase of Caenorhabditis elegans - Identification of the gene and demonstration of calcium and manganse transport. Journal of Biological Chemistry 276: 10683-10691 2001 ARTICLE CGC #4660 Kim J;Poole DS;Waggoner LE;Kempf A;Ramirez DS;Treschow PA;Schafer WR Genes affecting the activity of nicotinic receptors involved in Caenorhabditis elegans egg-laying behavior. Genetics 157: 1599-1610 2001 ARTICLE CGC #4661 Shioi G;Shoji M;Nakamura M;Ishihara T;Katsura I;Fujisawa H;Takagi S Mutations affecting nerve attachment of Caenorhabditis elegans. Genetics 157: 1611-1622 2001 ARTICLE CGC #4662 Delattre M;Felix MA Development and evolution of a variable left-right asymmetry in nematodes: The handedness of P11/P12 migration. Developmental Biology 232: 362-371 2001 ARTICLE CGC #4663 Engle MR;Singh SP;Nanduri B;Ji X;Zimniak P Invertebrate glutathione transferases conjugating 4-hydroxynonenal: CeGST 5.4 from Caenorhabditis elegans. Chemico-Biological Interactions 133: 244-248 2001 ARTICLE CGC #4664 van Rossum AJ;Jefferies J;Young CJ;Barrett J;Tait A;Brophy PM Glutathione S-transferase (GST) functional genomics: role of Caenorhabditis elegans in investigating GST expression in parasitic nematodes. Chemico-Biological Interactions 133: 274-277 2001 ARTICLE
CGC #4665 Thomas JH Nematodes are smarter then you think. Neuron 30: 7-8 2000 REVIEW CGC #4666 Gomez M;De Castro E;Guarin E;Sasakura H;Kuhara A;Mori I;Bartfai T;Bargmann CI;Nef P Ca2+ signaling via the neuronal calcium sensor-1 regulates associative learning and memory in C. elegans. Neuron 30: 241-248 2001 ARTICLE CGC #4667 Kitagawa H;Egusa N;Tamura J;Kusche-Gullberg M;Lindahl U;Sugahara K rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha 1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elogation of hepar Journal of Biological Chemistry 276: 4834-4838 2001 ARTICLE CGC #4668 Sternberg PW Working in the post-genomic C. elegans world. Cell 105: 173-176 2001 REVIEW CGC #4669 Sagasti A;Hisamoto N;Hyodo J;Tanaka-Hino M;Matsumoto K;Bargmann CI The CaMKII UNC-43 activates the MAPKKK NSY-1 to execute a lateral signaling decision required for asymmetric olfactory neuron fates. Cell 105: 221-232 2001 ARTICLE CGC #4670 Phelan P;Starich TA Innexins get into the gap. BioEssays 23: 388-396 2001 REVIEW CGC #4671 Schrimpf SP;Langen H;Gomes AV;Wahlestedt C A two-dimensional protein map of Caenorhabditis elegans. Electrophoresis 22: 1224-1232 2001 ARTICLE CGC #4672 Schisa JA;Pitt JN;Priess JR Analysis of RNA associated with P granules in germ cells of C. elegans adults. Development 128: 1287-1298 2001 ARTICLE
CGC #4673 Leroi AM Molecular signals versus the Loi de Balancement. Trends in Ecology &; Evolution 16: 24-29 2001 REVIEW CGC #4674 Luhn K;Wild MK;Eckhardt M;Gerardy-Schahn R;Vestweber D The gene defective in leukocyte adhesion deficiency II encodes a putative GDP-fucose transporter. Nature Genetics 28: 69-72 2001 ARTICLE CGC #4675 Wang W;Lim WA;Jakalian A;Wang J;Wang J;Luo R;Bayly CI;Kollman PA An analysis of the interactions between the Sem-5 SH3 domain and its ligands using molecular dynamics, free energy calculations, and sequence analysis. Journal of the American Chemical Society 123: 3986-3994 2001 ARTICLE CGC #4676 McCarter JP;Bird DM;Clifton SW;Waterston RH Nematode gene sequences, December 2000 update. Journal of Nematology 32: 331-333 2000 REVIEW CGC #4677 Ledent V;Vervoort M The basic helix-loop-helix protein family: Comparative genomics and phylogenetic analysis. Genome Research 11: 754-770 2001 ARTICLE CGC #4678 Delattre M;Felix MA Polymorphism and evoluton of vulval precursor cell lineages within two nematode genera, Caenorhabditis and Oscheius. Current Biology 11: 631-643 2001 ARTICLE CGC #4679 Habeos I;Papavassiliou AG Type 2 diabetes mellitus and worm longevity: a transcriptional link to cure? Trends in Endocrinology &; Metabolism 12: 139-140 2001 ARTICLE CGC #4680 Mikola J;Sulkava P Responses of microbial-feeding nematodes to organic matter distribution and predation in experimental soil habitat. Soil Biology &; Biochemistry 33: 811-817 2001 ARTICLE
CGC #4681 Marais G;Mouchiroud D;Duret L Does recombination improve selection on codon usage? Lessons from nematode and fly complete genomes. Proceedings of the National Academy of Sciences USA 98: 5688-5692 2001 REVIEW CGC #4682 Hekimi S;Benard C;Branicky R;Burgess J;Hihi AK;Rea S Why only time will tell. Mechanisms of Ageing &; Development 122: 571-594 2001 REVIEW CGC #4683 Braeckman BP;Houthoofd K;Vanfleteren JR Insulin-like signaling, metabolism, stress resistance and aging in Caenorhabditis elegans. Mechanisms of Ageing &; Development 122: 673-693 2001 ARTICLE CGC #4684 Funabashi Y;Kawamura K;Oshio K;Morita S;Osana Y;Akiyama E;Oka K Native response of C. elegans encoded in its neuron network. Journal of the Physical Society of Japan 70: 1154-1161 2001 ARTICLE CGC #4685 Du H;Chalfie M Genes regulating touch cell development in Caenorhabditis elegans. Genetics 158: 197-207 2001 ARTICLE CGC #4686 Yook KJ;Proulx SR;Jorgensen EM Rules of nonallelic noncomplementation at the synapse in Caenorhabditis elegans. Genetics 158: 209-220 2001 ARTICLE CGC #4687 van der Linden AM;Simmer F;Cuppen E;Plasterk RHA The G-protein B-subunit GPB-2 in Caenorhabditis elegans regulates the G(o)alpha-G(q)alpha signaling network through interactions with the regulator of G-protein signaling proteins EGL-10 and EAT-16. Genetics 158: 221-235 2001 ARTICLE CGC #4688 Sugimoto A;Kusano A;Hozak RR;Derry WB;Zhu J;Rothman JH Many genomic regions are required for normal embryonic programmed cell death in Caenorhabditis elegans. Genetics 158: 237-252 2001 ARTICLE
CGC #4689 Lee MH;Park H;Shim G;Lee J;Koo HS Regulation of gene expression, cellular localization, and in vivo function of Caenorhabditis elegans DNA topoisomerase I. Genes to Cells 6: 303-312 2001 ARTICLE CGC #4690 Wysocka J;Liu Y;Kobayashi R;Herr W Developmental and cell-cycle regulation of Caenorhabditis elegans HCF phosphorylation. Biochemistry 40: 5786-5794 2001 ARTICLE CGC #4691 Duerr JS;Gaskin J;Rand JB Identified neurons in C. elegans coexpress vesicular transporters for acetylcholine and monoamines. American Journal of Physiology - Cell Physiology 280: C1616-C1622 2001 ARTICLE CGC #4692 Pujol N;Link EM;Liu LX;Kurz CL;Alloing G;Tan MW;Ray KP;Solari R;Johnson CD;Ewbank JJ A reverse genetic analysis of components of the Toll signaling pathway in Caenorhabditis elegans. Current Biology 11: 809-821 2001 ARTICLE CGC #4693 Sommer RJ;Witte H;Schlak I Towards a microevolutionary approach in evolutionary developmental biology: Biogeography of the nematode genus Pristionchus (Diplogastridae). Zoology-Analysis of Complex Systems 103: 91-98 2001 ARTICLE CGC #4694 Sluder AE;Maina CV Nuclear receptors in nematodes: themes and variations. Trends in Genetics 17: 206-213 2001 ARTICLE CGC #4695 Ono S The Caenorhabditis elegans unc-78 gene encodes a homologue of actin-interacting protein 1 required for organised assembly of muscle actin filaments. Journal of Cell Biology 153: 889 2001 CORRECT
CGC #4696 Tajima T;Watanabe N;Kogawa Y;Takiguchi N;Kato J;Ikeda T;Kuroda A;Ohtake H Chemotaxis of the nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride. Journal of Bioscience &; Bioengineering 91: 322-324 2001 ARTICLE CGC #4697 Skop AR;Bergmann D;Mohler WA;White JG Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex. Current Biology 11: 735-746 2001 ARTICLE CGC #4698 Kwon S;Song WK;Park CS;Ahnn J Characterization of a novel gene expressed in neuromuscular tissues and centrosomes in Caenorhabditis elegans. Cell Biochemistry &; Function 19: 79-88 2001 ARTICLE CGC #4699 OConnell KF;Caron C;Kopish KR;Hurd DD;Kemphues KJ;Li Y;White JG The C. elegans zyg-1 gene encodes a regulator of centrosome duplication with distinct maternal and paternal roles in the embryo. Cell 105: 547-558 2001 ARTICLE CGC #4700 Wintle RF;Van Tol HHM Dopamine signaling in Caenorhabditis elegans - potential for parkinsonism research. Parkinsonism &; Related Disorders 7: 177-183 2001 REVIEW CGC #4701 Larsen PL Asking the age-old question. Nature Genetics 28: 102-104 2001 REVIEW CGC #4702 Lin K;Hsin H;Libina N;Kenyon C Regulation of the Caenorhabditis elegans longevity protein DAF-16 by insulin/IGF-1 and germline signaling. Nature Genetics 28: 139-145 2001 ARTICLE CGC #4703 Wicks SR;Yeh RT;Gish WR;Waterston RH;Plasterk RHA Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nature Genetics 28: 160-164 2001
ARTICLE CGC #4704 Zhou HM;Brust-Mascher I;Scholey JM Direct visualization of the movement of the monomeric axonal transport motor UNC-104 along neuronal processes in living Caenorhabditis elegans. Journal of Neuroscience 21: 3749-3755 2001 ARTICLE CGC #4705 Aronoff R;Baran R;Hodgkin J Molecular identification of smg-4, required for mRNA surveillance in C. elegans. Gene 268: 153-164 2001 ARTICLE CGC #4706 Tagawa A;Rappleye CA;Aroian RV pod-2, along with pod-1, defines a new class of genes required for polarity in the early Caenorhabditis elegans embryo. Developmental Biology 233: 412-424 2001 ARTICLE CGC #4707 Portereiko MF;Mango SE Early morphogenesis of the Caenorhabditis elegans pharynx. Developmental Biology 233: 482-494 2001 ARTICLE CGC #4708 Madi A;Kele Z;Janaky T;Punyiczki M;Fesus L Identification of protein substrates for transglutaminase in Caenorhabdits elegans. Biochemical &; Biophysical Research Communications 283: 964-968 2001 ARTICLE CGC #4709 Saeki S;Yamamoto M;Iino Y Plasticity of chemotaxis revealed by paired presentation of a chemoattractant and starvation in the nematode Caenorhabditis elegans. Journal of Experimental Biology 204: 1757-1764 2001 ARTICLE CGC #4710 Alper S;Kenyon C REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity. Development 128: 1793-1804 2001 ARTICLE
CGC #4711 Geles KG;Adam SA Germline and developmental roles of the nuclear transport factor importin alpha 3 in C. elegans. Development 128: 1817-1829 2001 ARTICLE CGC #4712 Gems D;Partridge L Insulin/IGF signalling and ageing: seeing the bigger picture. Current Opinion in Genetics &; Development 11: 287-292 2001 REVIEW CGC #4713 Chase DL;Patikoglou GA;Koelle MR Two RGS proteins that inhibit G alpha(o) and G alpha(q) signaling in C. elegans neurons require a G beta(5)-like subunit for function. Current Biology 11: 222-231 2001 ARTICLE CGC #4714 Robatzek M;Niacaris T;Steger K;Avery L;Thomas JH eat-11 encodes GPB-2, a G beta(5) ortholog that interacts with G(o)alpha and G(q)alpha to regulate C. elegans behavior. Current Biology 11: 288-293 2001 ARTICLE CGC #4715 Qin H;Rosenbaum JL;Barr MM An autosomal recessive polycystic kidney disease gene homolog is involved in intraflagellar transport in C. elegans ciliated sensory neurons. Current Biology 11: 457-461 2001 ARTICLE CGC #4716 Gumienny TL;Hengartner MO How the worm removes corpses: the nematode C. elegans as a model system to study engulfment. Cell Death &; Differentiation 8: 564-568 2001 REVIEW CGC #4717 Ankeny RA The natural history of Caenorhabditis elegans research. Nature Reviews Genetics 2: 474-479 2001 REVIEW CGC #4718 Grant B;Zhang Y;Paupard MC:Lin SX;Hall DH;Hirsh D Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recycling. Nature Cell Biology 3: 573-579 2001 ARTICLE
CGC #4719 Kayser EB;Morgan PG;Hoppel CL;Sedensky MM Mitochondrial expression and function of GAS-1 in Caenorhabditis elegans. Journal of Biological Chemistry 276: 20551-20558 2001 ARTICLE CGC #4720 Li Y;Dowbenko D;Lasky LA Caenorhabditis elegans PIAK, a phospholipid-independent kinase that activates the AKT/PKB survival kinase. Journal of Biological Chemistry 276: 20323-20329 2001 ARTICLE CGC #4721 van Swinderen B;Metz LB;Shebester LD;Mendel JE;Sternberg PW;Crowder CM G(o) alpha regulates volatile anesthetic action in Caenorhabditis elegans. Genetics 158: 643-655 2001 ARTICLE CGC #4722 Pasierbek P;Jantsch M;Melcher M;Schleiffer A;Schweizer D;Loidl J A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction. Genes &; Development 15: 1349-1360 2001 ARTICLE CGC #4723 Lindblom TH;Pierce GJ;Sluder AE A C. elegans orphan nuclear receptor contributes to xenobiotic resistance. Current Biology 11: 864-868 2001 ARTICLE CGC #4724 OQuinn AL;Wiegand EM;Jeddeloh JA Burkholderia pseudomallei kills the nematode Caenorhabditis elegans using an endotoxin-mediated paralysis. Cellular Microbiology 3: 381-393 2001 ARTICLE CGC #4725 Gotta M;Abraham MC;Ahringer J CDC-42 controls early cell polarity and spindle orientation in C. elegans. Current Biology 11: 482-488 2001 ARTICLE CGC #4726 Link EM;Hardiman G;Sluder AE;Johnson CD;Liu LX Therapeutic target discovery using Caenorhabditis elegans. Pharmacogenetics 1: 1-15 2000 REVIEW
CGC #4727 Altun-Gultekin Z;Andachi Y;Tsalik E L;Pilgrim D;Kohara Y;Hobert O A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23, controls cell fate specification of a defined interneuron class in C. elegans. Development 128: 1951-1969 2001 ARTICLE CGC #4728 Jornvall H;Shafqat J;Persson B Variations and constant patterns in eukaryotic MDR enzymes. Conclusions from novel structures and characterized genomes. Chemico-Biological Interactions 130-132: 491-498 2001 ARTICLE CGC #4729 Gibson G Developmental evolution: The unbearable likeness of beings. Current Biology 11: R345-R348 2001 REVIEW CGC #4730 Tavernarakis N;Everett JK;Kyrpides NC;Driscoll M Structural and functional features of the intracellular amino terminus of DEG/ENaC ion channels. Current Biology 11: R205-R208 2001 REVIEW CGC #4731 Easton A;Guven K;de Pomerai DI Toxicity of the dithiocarbamate fungicide Mancozeb to the nontarget soil nematode, Caenorhabditis elegans. Journal of Molecular Toxicology 15: 15-25 2001 ARTICLE CGC #4732 Mohsen AWA;Navarette B;Vockley J Identification of Caenorhabditis elegans isovaleryl-CoA dehydrogenase and structural comparison with other acyl-CoA dehydrogenases. Molecular Genetics &; Metabolism 73: 126-137 2001 ARTICLE CGC #4733 Boulianne GL Neuronal regulation of lifespan: clues from flies and worms. Mechanisms of Ageing &; Development 122: 883-894 2001 ARTICLE CGC #4734 Dernburg AF Here, there, and everywhere: Kinetochore function on holocentric chromosomes. Journal of Cell Biology 153: F33-F38 2001 REVIEW
CGC #4735 Moore LL;Roth MB HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for resolution of sister centromeres. Journal of Cell Biology 153: 1199-1207 2001 ARTICLE CGC #4736 Oegema K;Desai A;Rybina S;Kirkham M;Hyman AA Functional analysis of kinetochore assembly in Caenorhabditis elegans. Journal of Cell Biology 153: 1209-1225 2001 ARTICLE CGC #4737 Howe M;McDonald KL;Albertson DG;Meyer BJ HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. Journal of Cell Biology 153: 1227-1238 2001 ARTICLE CGC #4738 Vatamaniuk OK;Bucher EA;Ward JT;Rea PA A new pathway for heavy metal detoxification in animals: Phytochelatin synthase is required for cadmium tolerance in Caenorhabditis elegans. Journal of Biological Chemistry 276: 20817-20820 2001 ARTICLE CGC #4739 Ohtsuki T;Watanabe Y;Takemoto C;Kawai G;Ueda T;Kita K;Kojima S;Kaziro Y;Nyborg J;Watanabe K An "elongated" translation elongation factor Tu for truncated tRNAs in nematode mitochondria. Journal of Biological Chemistry 276: 21571-21577 2001 ARTICLE CGC #4740 Zang X;Maizels RM Serine proteinase inhibitors from nematodes and the arms race between host and pathogen. Trends in Biochemical Sciences 26: 191-197 2001 REVIEW CGC #4741 Cheeseman CL;Delany NS;Woods DJ;Wolstenholme AJ High-affinity ivermectin binding to recombinant subunits of the Haemonchus contortus glutamate-gated chloride channel. Molecular &; Biochemical Parasitology 114: 161-168 2001 ARTICLE CGC #4742 Kostrouchova M;Krause M;Kostrouch Z;Rall JE Nuclear hormone receptor CHR3 is a critical regulator of all four larval molts of the nematode Caenorhabditis elegans.
Proceedings of the National Academy of Sciences USA 98: 7360-7365 2001 ARTICLE CGC #4743 Parrish J;Li L;Klotz K;Ledwich D;Wang X;Xue D Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 412: 90-94 2001 ARTICLE CGC #4744 Hartman PS;Ishii N;Kayser EB;Morgan PG;Sedensky MM Mitochondrial mutations differentially affect aging, mutabiligy and anesthetic sensitivity in Caenorhabditis elegans. Mechanisms of Ageing &; Development 122: 1187-1201 2001 ARTICLE CGC #4745 Lee JS;Min DS;Park C;Park CS;Cho NJ Phytosphingosine and C2-phytoceramide induce cell death and inhibit carbachol-stimulated phospholipase D activation in Chinese hamster ovary cells expressing the Caenorhabditis elegans muscarinic acet FEBS Letters 499: 82-86 2001 ARTICLE CGC #4746 Suzuki N;Buechner M;Nishiwaki K;Hall DH;Nakanishi H;Takai Y;Hisamoto N;Matsumoto K A putative GDP-GTP exchange factor is required for development of the excretory cell in Caenorhabditis elegans. EMBO Reports 2: 530-535 2001 ARTICLE CGC #4747 Rogalski TM;Mullen GP;Bush JA;Gilchrist EJ;Moerman DG UNC-52/perlecan isoform diversity and function in Caenorhabditis elegans. Biochemical Society Transactions 29: 171-176 2001 ARTICLE CGC #4748 Ono K;Ohtomo T;Sato S;Sugamata Y;Suzuki M;Hisamoto N;Ninomiya-Tsuji J;Tsuchiya M;Matsumoto K An evolutionarily conserved motif in the TAB1 C-terminal region is necessary for interaction with and activation of TAK1 MAPKKK. Journal of Biological Chemistry 276: 24396-24400 2001 ARTICLE CGC #4749 Dodd RB;Drickamer K Lectin-like proteins in model organisms: implications for evolution of carbohydrate-binding activity. Glycobiology 11: 71R-79R 2001 ARTICLE
CGC #4750 Maruyama H;Rakow TL;Maruyama IN Synaptic exocytosis and nervous system development impaired in Caenorhabditis elegans unc-13 mutants. Neuroscience 104: 287-297 2001 ARTICLE CGC #4751 Reddien PW;Cameron S;Horvitz HR Phagocytosis promotes programmed cell death in C. elegans. Nature 412: 192-202 2001 ARTICLE CGC #4752 Hoeppner DJ;Hengartner MO;Schnabel R Engulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans. Nature 412: 202-206 2001 ARTICLE CGC #4753 Nuttley WM;Harbinder S;van der Kooy D Regulation of distinct attractive and aversive mechanisms mediating benzaldehyde chemotaxis in Caenorhabditis elegans. Learning &; Memory 8: 170-181 2001 ARTICLE CGC #4754 Walker GA;White TM;McColl G;Jenkins NL;Babich S;Candido EPM;Johnson TE;Lithgow GJ Heat shock protein accumulation is upregulated in a long-lived mutant of Caenorhabditis elegans. Journals of Gerontology Series A-Biological Sciences &; Medical Sciences 56: B281-B287 2001 ARTICLE CGC #4755 Kuwabara PE;Perry MD It aint over till its ova: germline sex determination in C. elegans. BioEssays 23: 596-604 2001 REVIEW CGC #4756 Miyakawa T;Yamada S;Harada S;Ishimori T;Yamamoto H;Hosono R Exposure of Caenorhabditis elegans to extremely low frequency high magnetic fields induces stress responses. Bioelectromagnetics 22: 333-339 2001 ARTICLE CGC #4757 Hofmann ER;Milstein S;Hengartner MO DNA-damage-induced checkpoint pathways in the nematode Caenorhabditis elegans. Cold Spring Harbor Symposia on Quantitative Biology 65: 467-473 2000
REVIEW CGC #4758 Grant WN;Viney ME Post-genomic nematode parasitology. International Journal for Parasitology 31: 879-888 2001 REVIEW CGC #4759 Hirabayashi J;Arata Y;Kasai K Glycome project: Concept, strategy and preliminary application to Caenorhabditis elegans. Proteomics 1: 295-303 2001 ARTICLE CGC #4760 Goldman RD Worms reveal essential functions for intermediate filaments. Proceedings of the National Academy of Sciences USA 98: 7659-7661 2001 REVIEW CGC #4761 Karabinos A;Schmidt H;Harborth J;Schnabel R;Weber K Essential roles for four cytoplasmic intermediate filament proteins in Caenorhabditis elegans development. Proceedings of the National Academy of Sciences USA 98: 7863-7868 2001 ARTICLE CGC #4762 Jiang H;Guo R;Powell-Coffman JA The Caenorhabditis elegans hif-1 gene encodes a bHLH-PAS protein that is required for adaptation to hypoxia. Proceedings of the National Academy of Sciences USA 98: 7916-7921 2001 ARTICLE CGC #4763 Richmond JE;Weimer RM;Jorgensen EM An open forum of syntaxin bypasses the requirement for UNC-13 in vesicle priming. Nature 412: 338-341 2001 ARTICLE CGC #4764 Spike CA;Shaw JE;Herman RK Analysis of smu-1, a gene that regulates the alternative splicing of unc-52 pre-mRNA in Caenorhabditis elegans. Molecular &; Cellular Biology 21: 4985-4995 2001 ARTICLE CGC #4765 McMahon L;Legouis R;Vonesch JL;Labouesse M Assembly of C. elegans apical junctions involves positioning and compaction by LET-413 and protein aggregation by the MAGUK protein DLG-1.
Journal of Cell Science 114: 2265-2277 2001 ARTICLE CGC #4766 Ramulu P;Nathans J Cellular and subcellular localization, N-terminal acylation, and calcium binding of Caenorhabditis elegans protein phosphatase with EF-hands. Journal of Biological Chemistry 276: 25127-25135 2001 ARTICLE CGC #4767 MacQueen AJ;Villeneuve AM Nuclear reorganization and homologous chromosome pairing during meiotic prophase require C. elegans chk-2. Genes &; Development 15: 1674-1687 2001 ARTICLE CGC #4768 Guerardel Y;Balanzino L;Maes E;Leroy Y;Coddeville B;Oriol R;Strecker G The nematode Caenorhabditis elegans synthesizes unusual O-linked glycans: identification of glucose-substituted mucin-type O-glycans and short chondroitin-like oligosaccharides. Biochemical Journal 357: 167-182 2001 ARTICLE CGC #4769 Hanazawa M;Mochii M;Ueno N;Kohara Y;Iino Y Use of cDNA subtraction and RNA interference screens in combination reveals genes required for germ-line development in Caenorhabditis elegans. Proceedings of the National Academy of Sciences USA 98: 8686-8691 2001 ARTICLE CGC #4770 Merz DC;Zheng H;Killeen MT;Krizus A;Culotti JG Multiple signaling mechanisms of the UNC-6/netrin receptors UNC-5 and UNC-40/DCC in vivo. Genetics 158: 1071-1080 2001 ARTICLE CGC #4771 Le QH;Turcotte K;Bureau T Tc8, a Tourist-like transposon in Caenorhabditis elegans. Genetics 158: 1081-1088 2001 ARTICLE CGC #4772 Grishok A;Pasquinelli AE;Conte D;Li N;Parrish S;Ha I;Baillie DL;Fire A;Ruvkun G;Mello CC Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing. Cell 106: 23-34 2001 ARTICLE
CGC #4773 Fallon PG;Allen RL;Rich T Primitive Toll signalling: bugs, flies, worms and man. Trends in Immunology 22: 63-66 2001 REVIEW CGC #4774 Ambros V Dicing up RNAs. Science 293: 811-813 2001 REVIEW CGC #4775 Hutvagner G;McLachlan J;Pasquinelli AE;Balint E;Tuschl T;Zamore PD A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838 2001 ARTICLE CGC #4776 Griffitts JS;Whitacre JL;Stevens DE;Aroian RV Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme. Science 293: 860-864 2001 ARTICLE CGC #4777 Pierce-Shimomura JT;Faumont S;Gaston MR;Pearson BJ;Lockery SR The homeobox gene lim-6 is required for distinct chemosensory representations in C. elegans. Nature 412: 566 2001 ARTICLE CGC #4778 Paupard MC;Miller A;Grant B;Hirsh D;Hall DH Immuno-EM localization of GFP-tagged yolk proteins in C. elegan using microwave fixation. Journal of Histochemistry &; Cytochemistry 49: 949-956 2001 ARTICLE CGC #4779 Clemens S;Schroeder JI;Degenkolb T Caenorhabditis elegans expresses a functional phytochelatin synthase. European Journal of Biochemistry 268: 3640-3643 2001 ARTICLE CGC #4780 Quintin S;Michaux G;McMahon L;Gansmuller A;Labouesse M The Caenorhabditis elegans gene lin-26 can trigger epithelial differentiation without conferring tissue specificity. Developmental Biology 235: 410-421 2001 ARTICLE
CGC #4781 Heid PJ;Raich WB;Smith R;Mohler WA;Simokat K;Gendreau SB;Rothman JH;Hardin J The zinc finger protein DIE-1 is required for late events during epithelial cell rearrangement in C. elegans. Developmental Biology 236: 165-180 2001 ARTICLE CGC #4782 Gotta M;Ahringer J Axis determination in C. elegans: initating and transducing polarity. Current Opinion in Genetics &; Development 11: 367-373 2001 REVIEW CGC #4783 Hope IA Broadcast interference - functional genomics. Trends in Genetics 17: 297-299 2001 REVIEW CGC #4784 Morita K;Shimizu M;Shibuya H;Ueno N A DAF-1-binding protein BRA-1 is a negative regulator of DAF-7 TGF-B signaling. Proceedings of the National Academy of Sciences USA 98: 6284-6288 2001 ARTICLE CGC #4785 Strome S;Powers J;Dunn M;Reese K;Malone CJ;White J;Seydoux G;Saxton W Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos. Molecular Biology of the Cell 12: 1751-1764 2001 ARTICLE CGC #4786 Matyash V;Geier C;Henske A;Mukherjee S;Hirsh D;Thiele C;Grant B;Maxfield FR;Kurzchalia TV Distribution and transport of cholesterol in Caenorhabditis elegans. Molecular Biology of the Cell 12: 1725-1736 2001 ARTICLE CGC #4787 Lehmann R Cell migration in invertebrates: clues from border and distal tip cells. Current Opinion in Genetics &; Development 11: 457-463 2001 REVIEW CGC #4788 Strauss E Longevity: Growing old together. Science 292: 41-43 2001 REVIEW
CGC #4789 Macdonald P Diversity in translational regulation. Current Opinion in Cell Biology 13: 326-331 2001 REVIEW CGC #4790 Park BJ;Lee JI;Lee J;Kim S;Cho KY;Park CS;Ahnn J Isolation of deletion mutants by reverse genetics in Caenorhabditis elegans. Korean Journal of Biological Sciences 5: 65-69 2001 ARTICLE CGC #4791 Kapitonov VV;Jurka J Rolling-circle transposons in eukaryotes. Proceedings of the National Academy of Sciences 98: 8714-8719 2001 ARTICLE CGC #4792 Nagendra HG;Harrington AE;Harmer NJ;Pellegrini L;Blundell TL;Burke DF Sequence analysis and comparative modeling of fly and worm fibroblast growth factor receptors indicate that the determinants for FGF and heparin binding are retained in evolution. FEBS Letters 501: 51-58 2001 ARTICLE CGC #4793 Gutzeit HO Biological effects of ELF-EMF enhanced stress response: New insights and new questions. Electro- and Magnetobiology 20: 15-26 2001 ARTICLE CGC #4794 Gamenara D;Pandolfi E;Saldana J;Dominguez L;Martinez MM;Seoane G Nematocidal activity of natural polyphenols from Bryophytes and their derivatives. Arzneimittel-Forschung 51: 506-510 2001 ARTICLE CGC #4795 Katti MV;Ranjekar PK;Gupta VS Differential distribution of simple sequence repeats in eukaryotic genome sequences. Molecular Biology &; Evolution 18: 1161-1167 2001 ARTICLE CGC #4796 Sanford C;Perry MD Asymmetrically distributed oligonucleotide repeats in the Caenorhabditis elegans genome sequence that map to regions important for meiotic chromosome segregation. Nucleic Acids Research 29: 2920-2926 2001
ARTICLE CGC #4797 De Stasio EA;Dorman S Optimization of ENU mutagenesis of Caenorhabditis elegans. Mutation Research-Genetic Toxicology &; Environmental Mutagenesis 495: 81-88 2001 ARTICLE CGC #4798 Hong L;Elbl T;Ward J;Franzini-Armstrong C;Rybicka KK;Gatewood BK;Baillie DL;Bucher EA MUP-4 is a novel transmembrane protein with functions in epithelial cell adhesion in Caenorhabditis elegans. Journal of Cell Biology 154: 403-414 2001 ARTICLE CGC #4799 Bercher M;Wahl J;Vogel BE;Lu C;Hedgecock ME;Hall DH;Plenefisch JD mua-3, a gene required for mechanical tissue integrity in Caenorhabditis elegans, encodes a novel transmembrane protein of epithelial attachment complexes. Journal of Cell Biology 154: 415-426 2001 ARTICLE CGC #4800 Cypser J;Johnson TE Hormesis extends the correlation between stress resistance and life span by long-lived mutants of Caenorhabditis elegans. Human &; Experimental Toxicology 20: 295-296 2001 ARTICLE CGC #4801 Ho SH;So GMK;Chow KL Postembryonic expression of Caenorhabditis elegans mab-21 and its requirement in sensory ray differentiation. Developmental Dynamics 221: 422-430 2001 ARTICLE CGC #4802 Goldstein B Embryonic polarity: a role for microtubules. Current Biology 10: R820-R822 2000 REVIEW CGC #4803 Polinko ES;Strome S Depletion of a Cks homolog in C. elegans embryos uncovers a post-metaphase role in both meiosis and mitosis. Current Biology 10: 1471-1474 2000 ARTICLE
CGC #4804 Johnson TE;Wu DQ;Tedesco P;Dames S;Vaupel JW Age-specific demographic profiles of longevity mutants in Caenorhabditis elegans show segmental effects. Journals of Gerontology A: 56-B331-B339 2001 ARTICLE CGC #4805 Jones SJM;Riddle DL;Pouzyrev AT;Velcules VE;Hillier L;Eddy SR;Strickli SL;Baillie DL;Waterston R;Marra MA Changes in gene-expression associated with developmental arrest and longevity in Caenorhabditis elegans. Genome Research 11: 1346-1352 2001 ARTICLE CGC #4806 Dwyer ND;Adler CE;Crump JG;LEtiole ND;Bargmann CI Polarized dendritic transport and the AP-1 mu-1 clathrin adapter UNC-101 localize odorant receptors to olfactory cilia. Neuron 31: 277-287 2001 ARTICLE CGC #4807 Gratzer H;Ahlf W Adjustment of a formulated sediment for sediment testing with Caenorhabditis elegans (Nematoda). Acta Hydrochimica et Hydrobiologica 29: 41-46 2001 ARTICLE CGC #4808 Yonemura I;Mabuchi I Heterogeneity of mRNA coding for Caenorhabditis elegans coronin-like protein. Gene 271: 255-259 2001 ARTICLE CGC #4809 Ranganathan R;Sawin ER;Trent C;Horvitz HR Mutations in the Caenorhabditis elegans serotonin reuptake transporter MOD-5 reveal serotonin-dependent and -independent activities of fluoxetine. Journal of Neuroscience 21: 5871-5884 2001 ARTICLE CGC #4810 Zhang Y;Grant B;Hirsh D RME-8, a conserved J-domain protein, is required for endocytosis in Caenorhabditis elegans. Molecular Biology of the Cell 12: 2011-2021 2001 ARTICLE CGC #4811 Zipperlen P;Fraser AG;Kamath RS;Martinez-Campos M;Ahringer J Roles for 147 embryonic lethal genes on C. elegans chromosome I identified by RNA interference and video microscopy.
EMBO Journal 20: 3984-3992 2001 ARTICLE CGC #4812 Salcine AE;Hilliard MA;Croce A;Arbucci S;Luzzi P;Tacchett C;Daniell L;Decamill P;Pelicci PG;Difiore PP The EPS15 C. elegans homolog ehs-1 is implicated in synaptic vesicle recycling. Nature Cell Biology 3: 755-760 2001 ARTICLE CGC #4813 Tsang WY;Sayles LC;Grad LI;Pilgrim DB;Lemire BD Mitochondrial respiratory chain deficiency in Caenorhabditis elegans results in developmental arrest and increased life span. Journal of Biological Chemistry 276: 32240-32246 2001 ARTICLE CGC #4814 Chong H;Lee J;Guan KL Positive and negative regulation of Raf kinase activity and function by phosphorylation. EMBO Journal 20: 3716-3727 2001 ARTICLE CGC #4815 Wagner A Birth and death of duplicated genes in completely sequenced eukaryotes. Trends in Genetics 17: 237-239 2001 REVIEW CGC #4816 Rodaway A;Patient R Mesendoderm: An ancient germ layer? Cell 105: 169-172 2001 REVIEW CGC #4817 Kenyon C A conserved regulatory system for aging. Cell 105: 165-168 2001 REVIEW CGC #4818 Stearns T Centrosome duplication: a centriolar pas de deux. Cell 105: 417-420 2001 REVIEW CGC #4819 Lynch M;Conery JS The evolutionary fate and consequences of duplicate genes. Science 290: 1151-1155 2001
ARTICLE CGC #4820 Espinosa F;Fleischhauer R;McMahon A;Joho RH Dynamic interaction of S5 and S6 during voltage-controlled gating in a potassium channel. Journal of General Physiology 118: 157-169 2001 ARTICLE CGC #4821 Hashmi S;Tawe W;Lustigman S Caenorhabditis elegans and the study of gene function in parasites. Trends in Parasitology 17: 387-393 2001 REVIEW CGC #4822 Yashin AI;Cypser JR;Johnson TE;Michalski AI;Boyko SI;Novoseltsev VN Ageing and survival after different doses of heat shock: the results of analysis of data from stress experiments with the nematode worm Caenorhabditis elegans. Mechanisms of Ageing &; Development 122: 1477-1495 2001 ARTICLE CGC #4823 Sugawara K;Morita K;Ueno N;Shibuya H BIP, a BRAM-interacting protein involved in TGF-B signalling, regulates body length in Caenorhabditis elegans. Genes to Cells 6: 599-606 2001 ARTICLE CGC #4824 Grandien K;Sommer RJ Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequences. Genes &; Development 15: 2161-2172 2001 ARTICLE CGC #4825 Dozier C;Kagoshima H;Niklaus G;Cassata G;Burglin TR The Caenorhabditis elegans Six/sine oculis class homeobox gene ceh-32 is required for head morphogenesis. Developmental Biology 236: 289-303 2001 ARTICLE CGC #4826 Johnson AD;Fitzsimmons D;Hagman J;Chamberlin HM EGL-38 Pax regulates the ovo-related gene lin-48 during Caenorhabditis elegans organ development. Development 128: 2857-2865 2001 ARTICLE
CGC #4827 Goodwin EB Translational repression: Not just a Puf of smoke. Current Biology 11: R607-R609 2001 REVIEW CGC #4828 Wendland B Round-trip ticket: recycling to the plasma membrane requires RME-1. Nature Cell Biology 3: E133-E135 2001 REVIEW CGC #4829 George AL;Bianchi L;Link EM;Vanoye CG >From stones to bones: the biology of ClC channels. Current Biology 11: R620-R628 2001 REVIEW CGC #4830 Lin SX;Grant B;Hirsh D;Maxfield FR Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cells. Nature Cell Biology 3: 567-572 2001 ARTICLE CGC #4831 Caplen NJ;Parrish S;Imani F;Fire A;Morgan RA Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proceedings of the National Academy of Sciences USA 98: 9742-9747 2001 ARTICLE CGC #4832 Chen PJ;Cho S;Jin SW;Ellis RE Specification of germ cell fates by FOG-3 has been conserved during nematode evolution. Genetics 158: 1513-1525 2001 ARTICLE CGC #4833 Koh K;Rothman JH ELT-5 and ELT-6 are required continuously to regulate epidermal seam cell differentiation and cell fusion in C. elegans. Development 128: 2867-2880 2001 ARTICLE CGC #4834 Ueda T;Katsuzaki H;Terami H;Ohtsuka H;Kagawa H;Murase T;Kajiwara Y;Yoshioka O;Iio T Calcium-bindings of wild type and mutant troponin Cs of Caenorhabditis elegans. Biochemica et Biophysica Acta-Protein Structure &; Molecular Enzymology 1548: 220-228 2001
ARTICLE CGC #4835 Ghenea S;Takeuchi M;Motoyama J;Sasamoto K;Kunau WH;Kamiryo T;Bun-ya M The cDNA sequence and expression of the AAA-family peroxin genes pex-1 and pex-6 from the nematode Caenorhabditis elegans. Zoological Science 18: 675-681 2001 ARTICLE CGC #4836 Brockie PJ;Mellem JE;Hills T;Madsen DM;Maricq AV The C. elegans glutamate receptor subunit NMR-1 is required for slow NMDA-activated currents that regulate reversal frequency during locomotion. Neuron 31: 617-630 2001 ARTICLE CGC #4837 Bessereau JL;Wright A;Williams DC;Schuske K;Davis MW;Jorgensen EM Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line. Nature 413: 70-74 2001 ARTICLE CGC #4838 Zhao H;Nonet ML A conserved mechanism of synaptogyrin localization. Molecular Biology of the Cell 12: 2275-2289 2001 ARTICLE CGC #4839 Westmoreland JJ:McEwen J;Moore BA;Jin Y;Condie BG Conserved function of Caenorhabditis elegans UNC-30 and mouse Pitx2 in controllig GABAergic neuron differentiation. Journal of Neuroscience 21: 6810-6819 2001 ARTICLE CGC #4840 Chen L;Ong B;Bennett V LAD-1, the Caenorhabditis elegans L1CAM homologue, participates in embryonic and gonadal morphogenesis and is a substrate for fibroblast growth factor receptor pathway-dependent phosphotyrosine-based Journal of Cell Biology 154: 841-855 2001 ARTICLE CGC #4841 Edens WA;Sharling L;Cheng G;Shapira R;Kinkade JM;Lee T;Edens HA;Tang X;Sullards C;Flaherty DB;Benian GM;Lambeth JD Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a multidomain oxidase/peroxidase with homology to the phagocyte oxidase subunit gp91 phox. Journal of Cell Biology 154: 879-891 2001 ARTICLE
CGC #4842 Oka T;Toyomura T;Honjo K;Wada Y;Futai M Four subunit alpha isoforms of Caenorhabditis elegans vacuolar H+-ATPase Cell-specific expression during development. Journal of Biological Chemistry 276: 33079-33085 2001 ARTICLE CGC #4843 Kawamura K;Funabashi Y;Morita S;Omata K;Oshio K;Osana Y;Oka K Does a randomly organized electrical circuit function as a neuronal system? Japanese Journal of Applied Physics Part 1 40: 2095-2099 2001 ARTICLE CGC #4844 Delattre M;Felix MA Microevolutionary studies in nematodes: a beginning. BioEssays 23: 807-819 2001 REVIEW CGC #4845 Takahashi M;Asaumi S;Honda S;Suzuki Y;Nakai D;Kuroyanagi H;Shimizu T;Honda Y;Shirasawa T Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans. Biochemical &; Biophysical Research Communications 286: 534-540 2001 ARTICLE CGC #4846 Gonczy P;Grill S;Stelzer EHK;Kirkham M;Hyman AA Spindle positioning during the asymmetric first cell division of Caenorhabditis elegans. Novart Foundation 237: 164-181 2001 ARTICLE CGC #4847 Ambros V The temporal control of cell cycle and cell fate in Caenorhabditis elegans. Novart Foundation 237: 203-220 2001 REVIEW CGC #4848 Harris TW;Schuske K;Jorgensen M Studies of synaptic vesicle endocytosis in the nematode C. elegans. Traffic 2: 597-605 2001 ARTICLE CGC #4849 Kim SK HTTP://C. elegans: Mining the functional genomic landscape. Nature Reviews Genetics 2: 681-689 2001 REVIEW
CGC #4850 Rougvie AE Control of developmental timing in animals. Nature Reviews Genetics 2: 690-701 2001 REVIEW CGC #4851 Masler EP;Kovaleva ES;Sardanelli S Aminopeptidase-like activities in Caenorhabditis elegans and the soybean cyst nematode, Heterodera glycines. Journal of Helminthology 75: 267-272 2001 ARTICLE CGC #4852 Tavernarakis N;Driscoll M Mechanotransduction in Caenorhabditis elegans - the role of DEG/ENAC ion channels. Cell Biochemistry and Biophysics 35: 1-18 2001 REVIEW CGC #4853 Kwon JY;Lee J Biological significance of a universally conserved transcription mediator in metazoan developmental signaling pathways. Development 128: 3095-3104 2001 ARTICLE CGC #4854 Barr MM;DeModena J;Braun D;Nguyen CQ;Hall DH;Sternberg PW The Caenorhabditis elegans autosomal dominant polycystic kidney disease gene homologs lov-1 and pkd-2 act in the same pathway. Current Biology 11: 1341-1346 2001 ARTICLE CGC #4855 Altmann F;Fabini G;Ahorn H;Wilson IBH Genetic model organisms in the study of N-glycans. Biochimie 83: 703-712 2001 REVIEW CGC #4856 Kim SK;Lund J;Kiraly M;Duke K;Jiang M;Stuart JM;Eizinger A;Wylie BN;Davidson GS A gene expression map for Caenorhabditis elegans. Science 293: 2087-2092 2001 ARTICLE CGC #4857 Garsin DA;Sifri CD;Mylonakis E;Qin X;Singh KV;Murray BE;Calderwood SB;Ausubel FM A simple model host for identifying Gram-positive virulence factors. Proceedings of the National Academy of Sciences USA 98: 10892-10897 2001 ARTICLE
CGC #4858 Park BJ;Lee DG;Yu JR;Jung SK;Choi K;Lee J;Kim YS;Lee JI;Kwon JY;Singson A;Song WK;Eom SH;Park CS;Kim DH;Bandyopadhyay J;Ahnn J Calreticulin, a calcium-binding molecular chaperone, is required for stress response and fertility in Caenorhabditis elegans. Molecular Biology of the Cell 12: 2835-2845 2001 ARTICLE CGC #4859 Cinar HN;Sweet KL;Hosemann KE;Earley K;Newman AP The SEL-12 presenilin mediates induction of the Caenorhabditis elegans uterine pi cell fate. Developmental Biology 237: 173-182 2001 ARTICLE CGC #4860 Marks NJ;Shaw C;Halton DW;Thompson DP;Geary TG;Li C;Maule AG Isolation and preliminary biological assessment of AADGAPLIRFamide and SVPGVLRFamide from Caenorhabditis elegans. Biochemical &; Biophysical Research Communications 286: 1170-1176 2001 ARTICLE CGC #4861 Morrison GE;van der Kooy D A mutation in the AMPA-type glutamate receptor, glr-1, blocks olfactory associative and nonassociative learning in Caenorhabditis elegans. Behavioral Neuroscience 115: 640-649 2001 ARTICLE CGC #4862 Darby C;Falkow S Mimicry of a G protein mutation by pertussis toxin expression in transgenic Caenorhabditis elegans. Infection and Immunity 69: 6271-6275 2001 ARTICLE CGC #4863 Link CD Transgenic invertebrate models of age-associated neurodegenerative diseases. Mechanisms of Ageing and Development 122: 1639-1649 2001 REVIEW CGC #4864 Ohno S Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity. Current Opinion in Cell Biology 13: 641-648 2001 REVIEW CGC #4865 Schierenberg E Three sons of fortune: early embryogenesis, evolution and ecology of nematodes.
BioEssays 23: 841-847 2001 REVIEW CGC #4866 Kelley S Getting started with Acedb. Briefings in Bioinformatics 1: 131-137 2001 REVIEW CGC #4867 Tavernarakis N;Driscoll M Degenerins. At the core of the metazoan mechanotransducer? Annals of the New York Academy of Sciences 940: 28-41 2001 REVIEW CGC #4868 Meyer BJ Sex and death of a worm: assessing and repressing X chromosomes. Harvey Lectures 95: 85-105 2000 REVIEW CGC #4869 Guarente L SIR2 and aging - the exception that proves the rule. Trends in Genetics 17: 391-392 2001 REVIEW CGC #4870 Knight SW;Bass BL A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 293: 2269-2271 2001 ARTICLE CGC #4871 Ozhogina OA;Trexler M;Banyai L;Llinas M;Patthy L Origin of fibronextin type II (FN2) modules: Structural analyses of distantly-related members of the kringle family identify the kringle domain of neurotrypsin as a potential link between FN2 domains Protein Science 10: 2114-2122 2001 ARTICLE CGC #4872 Morita S;Oshio K;Osana Y;Funabashi Y;Oka K;Kawamura K Geometrical structure of the neuronal network of Caenorhabditis elegans. Physica A 298: 553-561 2001 ARTICLE CGC #4873 Jasmer DP;Roth J;Myler PJ Cathespin B-like cystein proteases and Caenorhabditis elegans homologues dominate gene products expressed in adult Haemonchus contortus intestine. Molecular &; Biochemical Parasitology 116: 159-169 2001
ARTICLE CGC #4874 Majewska A;Yuste R Topology of gap junction networks in C. elegans. Journal of Theoretical Biology 212: 155-167 2001 ARTICLE CGC #4875 Kurokawa H;Osawa M;Kurihara H;Katayama N;Tokumitsu H;Swindells MB;Kainosho M;Ikura M Target-induced conformational adaptation of calmodulin revealed by the crystal structure of a complex with nematode Ca2+/calmodulin-dependent kinase kinase peptide. Journal of Molecular Biology 312: 59-68 2001 ARTICLE CGC #4876 Ishiguro H;Yasuda K;Ishii N;Ihara K;Ohkubo T;Hiyoshi M;Ono K;Senoo-Matsuda N;Shinohara O;Yosshii F;Murakami M;Hartman PS;Tsuda M Enhancement of oxidative damage to cultured cells and Caenorhabditis elegans by mitochondrial electron transport inhibitors. Iubmb Life 51: 263-268 2001 ARTICLE CGC #4877 Friesen JA;Liu MF;Kent C Cloning and characterization of a lipid-activated CTP: phosphocholin cytidylyltransferase from Caenorhabditis elegans: identification of a 21-residue segment critical for lipid activation. Biochimica et Biophysica Acta-Molecular &; Cell Biology of Lipids 1533: 86-98 2001 ARTICLE
WormBase Update
WormBase Consortium www.wormbase.org Release schedule One of the most important improvements to WormBase over the past 12 months has been a drastic increase in the frequency of database updates. WormBase is built in two stages. In the first stage, a complete database (ACeDB) is built incorporating changes and additions from all WormBase sites. This is done weekly, and unless there is a major problem, can be downloaded weekly. The version of the database is listed on the WormBase homepage as WS#, e.g., WS56 for the September 28, 2001 update to WormBase site. In the second stage, the database is configured to support the wormbase.org website. This is now done about every two weeks. We are close to having mirror sites at the Sanger Centre and Caltech; see the WormBase homepage for details. User Interface The WormBase user interface is still very much evolving. Some of the changes are: Genome Browser. The genome browser Genome Hunter has been updated to show predicted and confirmed genes, the precise endpoints of cosmids and YACs, ESTs aligned by BLAT (see below), the regions of genomic sequence corresponding to genes defined by the Worm Transcriptome Projects analysis of Y. Koharas ESTs, regions of homology to C. briggsae, regions with Prosite domains, the oligos and regions they amplify that have been used in some microarray and RNAi experiments, ESTs, among other features. These features are color-coded in the display. You can check boxes to specify the features you would like to see. BLAT is a sequence alignment program written by W.J. Kent at UC Santa Cruz. It efficiently scans a pair of DNA sequences for small regions of high identity: those 40 or more bases long with 95% identity, or perfect sequence matches down to 33 bases in length. It is highly useful for aligning cDNAs to genomic DNA, or small genomic fragments to a genome draft. Genetic Map Viewer. The new genetic map viewer that became available this past spring is Java-based and still not compatible with Macintoshs. We therefore enhanced the classic acedb graphic map display to make it easier to navigate. A new more web-friendly viewer is under development. Search pages Genetic Interval Search. A new genetic Interval Search page takes advantage of the interpolation of genetic and physical maps at the resolution of individual clones. This search allows you to specify a range by map position, gene name, or clone, and returns a list of genes in that region. After determining the range, this script lists all mapped mutants within the range as well as predicted genes on clones that have been interpolated into the range. Of course, since not all genetic loci are mapped relative to one another, the order of genetic loci presented in chromosomal coordinates may not actually reflect the physical order of these genes.
RNAi Phenotype Search. An RNAi search page allows you to search for genes for which RNAi experiments have been done. Most of these are from the large scale projects published in the past year, and an increasing set from individual papers. Negative data from all but the EMBL screen are included. New data RNAi. In addition to the 147 movies from RNAi experiments from the Ahringer laboratory (Zipperlen et al., 2001) are now included in WormBase. Expression patterns from papers. We are focusing on extracting gene expression patterns from the 4630 papers in the CGC bibliography. We almost half done, and now have with 1297Expr patterns representing about 518 genes. In general, each experiment or cluster of related experiments is described in one Expr object. For example, if a genes expression has been analyzed by GFP fusions and by antibodies, there will be two Expr objects in wormbase WTP genes. The regions corresponding to over 10,000 genes from the Worm Transcriptome Projects analysis of EST sequences have been added this summer. The Thierry-Miegs might have additional information on splicing patterns of individual genes, and you should email them for more information. WormPep. WormPep is a set of current best inferences about proteins encoded in the C. elegans genome. Since WormPep is now revised weekly, you can obtain the data for previous versions at http://www.sanger.ac.uk/Projects/C_elegans/wormpep/. Coming Soon SNPs. The positions of the Washington University SNPs will be included in the Genome Viewer. Deletions from the C. elegans Knockout Consortium will be indicated in the genome viewer and on the Gene report pages. C. briggsae data. The assembled genomic sequence from C. briggsae generated by 10x coverage shotgun sequencing at the Sanger Centre and at the Washington University Genome Sequence Center should be in WormBase this Fall. Microarray data. We have started with Stuart Kims global analysis of gene expression that clusters genes over about 500 experiments. Other data will be added in the near future. Gene Ontology Consortium WormBase has joined the Gene Ontology (GO) Consortium. GO is a structured vocabulary allowing the biological functions of gene products to be described with arbitrarily high levels of detail, and compared between diverse organisms in a way independent of sequence similarity or idiosyncrasies of a given model system. More details are available at http://www.geneontology.org. We have begun incorporating GO terms into Wormbase. The first step was to automatically generate annotations based on the Interpro repository of protein sequence motifs, which has become the standard for computational annotation of protein-encoding portions of whole genomes (e.g., the Arabidopsis and human genomes). A second step, currently underway, is to automatically map GO terms onto ~800 genes with mass-produced RNAi phenotypes. This is being done in collaboration with the WormPD database at Proteome, Inc. The longer term and most important phase is to manually annotate each gene with GO terms. During all of this, it is continually necessary to invent new GO terms specifically fitted to the biology of C. elegans. Another basic requirement is to develop a logical scheme (ontology) relating parts of the anatomy; this is being done in collaboration with David Hall and Zeynep Altun of the Worm Atlas Project.
Sheldon McKay 1 , Mark Edgley 1 , Erin Gilchrist 1 , Steven Jones 2 , Don Moerman 1,3 elegans Reverse Genetics Core Facility, Biotechnology Laboratory, University of British Columbia, Vancouver, BC 2 Genome Sequence Centre, British Columbia Cancer Agency, Vancouver, BC 3 Department of Zoology, University of British Columbia, Vancouver, BC
The Vancouver node of the C. elegans gene knockout consortium has made information on the gene knockout process available on the web (http://ko.cigenomics.bc.ca/protocols.html) for researchers who wish to perform gene knockout experiments in their own labs. The time-line and methods for the gene knockout process is described, from mutagenesis to isolation of individual mutations. The information also includes a detailed description and experimental protocols for PCR screening of mutagenized libraries using the "poison primer" technique. Our library construction methods are modeled after the Moulder and Barstead protocol (http://www.mutantfactory.ouhsc.edu/protocols.asp), while our mutagenesis protocol is modified from the method developed by Shohei Mitani. We have also released an updated version of a web-accessible program that uses information in ACeDB to design PCR primers for gene knockout experiments (http://ko.cigenomics.bc.ca/oligos.shtml) and a searchable database of pre-designed knockout primer sets for standard and "poison primer" PCR screening (http://ko.cigenomics.bc.ca/oligodb.shtml).
One of the limitations of C. elegans transgene studies has been the difficulty in obtaining stable (i.e., integrated), low copy number lines. Praitis et al. (Genetics 2001, 157:1217-26) have adapted biolistic transformation (bombardment with DNA-coated gold microparticles) to worms for this purpose by selecting for rescue of unc-119(ed3) lethality on starved plates. The identification of selectable markers in addition to unc-119(ed3)would facilitate the construction of lines with multiple integrated transgenes. In a pilot experiment, we attempted to rescue the temperature-sensitive, sperm-specific sterility (Spe) of the spe-26(hc138ts) mutation via biolistic transformation. We used the spe-26 plasmid pJV145, which, by microinjection, complements the Spe phenotype. Synchronized populations of spe-26(hc138ts) were grown at 15 degrees until young adulthood, subjected to bombardment, allowed to recover for two hours, transferred to new plates, then shifted to 25 degrees. Otherwise, conditions for bombardment were identical to the published protocol. The large number of worms quickly depleted the available OP50, so we added an OP50 "soup" (1ml of a centrifuged culture containing ~50% bacteria by volume) every day or two as needed to prevent dauer arrest. F1 progeny of the bombarded worms should be sterile unless rescued. We obtained four independent fertile lines from 20 bombardments. PCR screening of single fertile worms with plasmid-specific primers confirmed its presence in all four lines. In three of the lines, the high frequency of sterility in subsequent generations suggested extrachromosomal maintenance; those lines were not characterized further. The fourth line exhibited stable maintenance of fertility, and Southern blot analysis confirmed the integration of a single copy of the transgene. Current efforts include testing of a different spe-26 allele (it112ts) and different bombardment parameters to increase the efficiency of integrated rescue.
Primers: A= 5 upstream, approx.20-25 nt A* = nested to A (3-10 bp away from A B = spanning 20-24 nt of end of gene to fuse + 24 nt of gfp-vector pPD95.75 (= sequence of the PLUS strand: 5-AGCTTGCATGCCTGCAGGTCGACT-3). Example: If the 3end of the gene/promoter has the plus strand sequence 5-agagagagagagagagagagag-3, the whole primer B would be: 5- AGTCGACCTGCAGGCATGCAAGCTctctctctctctctctctctct-3 C = polylinker beginning of pPD95.75: 5-AGCTTGCATGCCTGCAGGTCGACT-3 D=at the end of unc-54 3UTR: 5AAGGGCCCGTACGGCCGACTAGTAGG-3 D*= immediately nested to D: 5-GGAAACAGTTATGTTTGGTATATTGGG-3
Overexpression of UNC-40 in the touch neurons results in misdirection of the ALM axon.
Naomi Levy-Strumpf, Joseph G. Culotti S.L. Research Institute of Mt. Sinai Hospital, 600 University Ave., Toronto M5G 1X5 Canada Ectopic expression of UNC-5 in the touch neurons leads to reorientation of their axons toward the dorsal side in an unc-6 dependent manner (1). Therefore, the expression of UNC-5 changes their responsiveness to the UNC-6 signal from attraction to repulsion in the case of the AVM and PVM, and from non-responsiveness to repulsion in the case of the ALM. The latter suggests that the ALM contains the necessary machinery to respond to the UNC-6 signal, but is refractory to UNC-6 and sends a process longitudinally rather than ventrally. Several reasons might account for this, including that UNC-40, which is expressed in the ALM, is tightly regulated and therefore is prevented from responding to the UNC-6 ventral signal or that the forces that drive the ALM process longitudinally are dominant over the UNC-6 ventral cue. We have overexpressed an UNC-40gfp fusion protein in the touch neurons by using the mec-7 promoter. The overexpression of UNC-40 in the ALM results in a variety of axon reorientations which we intend to pursue further. The most common phenotype we observe is that the ALM process, which normally migrates longitudinally, reorients itself towards the ventral cord before it reaches the nerve ring, suggesting that it became responsive to the proximal UNC-6 signal expressed by the axon tracts entering the nerve ring. This finding suggests that the overexpression of UNC-40 in the ALM increases its sensitivity to UNC-6 and prompted us to initiate a screen to look for mutants in which the ALM sends a process ventrally at an earlier stage. Such further increase in the sensitivity to UNC-6 may result from mutations in genes that function either in the regulation of UNC-40 or in the opposing forces that prevent the ALM from sending its process ventrally . 1. Expression of the UNC-5 guidance receptor in the touch neurons of C. elegans steers their axons dorsally. Hamelin et al. Nature 364: 327-330 (1993).
Understanding the function of egl-38 and lin-48 using heat shock promoters hsp16-2 and hsp16-41
Ashwin Uttam, Helen Chamberlin 941 Bio Science Bldg. Columbus OH. 43210 In C.elegans studies of hindgut development indicate the EGL-38 Pax transcription factor directly regulates lin-48, a gene encoding an Ovo related zinc finger transcription factor. To better understand the function of these two genes, we have made clones that include a heat shock promoter (hsp 16-2 and hsp 16-41) and cDNA for each gene. These clones will allow us to test the effect of ectopic expression for each gene. Since egl-38 and lin-48 are initially expressed in embryos, we wanted to test the effect of heat shock on embryos. In preliminary experiments, we observed embryos are very sensitive to the commonly used conditions of 33 C for 1+ hours. Consequently, we wanted to identify a heat shock (HS) condition that could both induce heat shock expression and minimize lethality. To identify a good heat shock condition, a gfp cDNA was cloned into the hsp16-2 and hsp 16-41 promoter vectors. After the injection of the animals and the production of transgenic lines, the animals were heat shocked under various conditions (Table 1.1). We found the greatest gfp expression with minimum amount of lethality due to the heat shock was observed when the animals were incubated at 35 C for half an hour. The above heat shock conditions were also not too detrimental for the animals if they were heat shocked everyday for 24 hours through out their developing life. HS conditions Clone % lethality after HS% lethality w/out HSgfp expression 33 C for 1 hour hsp16-2::gfp 7% 6% +/33 C for 1 hour hsp16-41::gfp 9% 5% +/35 C for 1 hour hsp16-2::gfp 39% 7% + 35 C for 1 hour hsp16-41::gfp 55% 0% + 35 C for hourhsp16-2::gfp 9% 1% + 35 C for hourhsp16-41::gfp 6% 5% + Table 1.1 These conditions were then used on transgenic animals carrying a clone with hsp16-2::egl-38, and animals with hsp16-41::lin-48. Although we found neither transgene significantly affected embryonic variability, both affected adult male spicule development (Table 1.2), hsp16-2::egl-38 also affects larvae viability. Genetic results indicate that lin-48 plays an inhibitory role in the development of the male spicules because lin-48 mutant males have ectopic spicule-like structures. The similarity in effect for egl-38 and lin-48 is most likely due to the fact that egl-38 is a transcription factor for lin-48. Therefore, over expressing egl-38 results in an over expression of lin-48, and there by yielding the deformed or absent male spicule phenotype. % abnormal males% abnormal males after HS w/out HS hsp16-2::lin-48 59% 1.6% Control 3% 0% Table 1.2 Clone
Suppressor Screen of egl-38 egg-laying defect to study the vulva to uterus signalling pathway
Vandana Rajakumar, Helen M Chamberlin MCDB Graduate Program, Department of Molecular Genetics, 941, Biological Sciences Building, The Ohio State University,Columbus, OH 43210 Communication between tissues in an organ system during their development is vital to ensure that they function as a unit. The egg-laying system in Caenorhabditis elegans offers a good model system to study this paradigm. Here the reciprocal interactions from the AC to the vulva and from the vulva to the uterus coordinate development between these tissues. The AC initially induces 3 of 6 multipotential Vulval Precursor Cells (VPCs) to form the vulval cells by the LIN-3-LET-23 mediated signalling pathway. Then the 1 vulval cells reciprocally signal to the uterine cells to induce uv 1 cell formation via LIN-3 again. Some genes important for the AC to vulva signal also mediate the vulva to uterus signal, whereas others function in only one or the other developmental pathway. For example, only the vulval transcription of lin-3 requires EGL-38, a PAX transcription factors while the earlier AC expression is EGL-38- independent (1). Although the AC to vulva signal pathway has been studied in detail, the vulva to uterus pathway is less well characterized. To identify additional genes in the reciprocal pathway from the vulva to the uterus, we have devised a mutagenesis screen for temperature sensitive supressors using egl-38(n578) animals. These animals are egg-laying defective (2,3). We reasoned that mutations in genes that act as negative regulators of the reciprocal pathway may supress the egg-laying defect. Identification and characterization of these suppressor genes would enable us to better understand at a molecular level, the signalling pathway occuring between tissues during their development to form an organ system. We have carried out two genetic screens using EMS as a mutagen wherein the mutagenized animals are subjected to different temperatures during their development. To identify mutations in genes that may have essential additional functions one screen incorporates a temperature shift during the egg-laying system development: 1.Screen 1: Mutagenized worms allowed to grow at 15 C throughout their development 18,783 gametes were screened and eight candidate supressors were recovered. Two supress at all temperatures, five are cold sensitive and one supresses at 20 C. 2.Screen 2: Mutagenized worms allowed to grow at 15 C at all stages but exposed t 25 C during vulval development.12,375 gametes were screened, wherein five candidate supressors were isolated.Two have been verified to be strong supressors at 15 C while two more are weaker. One candidate seems to require exposure to both 15 C and 25 C during development to exhibit supressor phenotype.Results from screening another 12,408 gametes are still awaited. 1. Chang, C., Newman, A.P and Sternberg, P.W. (1999) Current Biology 9: 237-246 2. Trent.C., Tsung ,N and Horvitz, H.R (1983) Genetics 104 : 619-647 (3) Chamberlin, H.M., Palmer, R.E., Newman, A.P., et al (1997) Development 124: 3919-3928
Comparative study of the ovo-related gene lin-48 provides evidence for the evolution of transcriptional regulation
Xiaodong Wang, Helen M. Chamberlin Department of Molecular Genetics, Ohio State University, Columbus, OH 43210 Comparative analysis shows that proteins can be conserved entities throughout the animal kingdom, but their regulation can change during the evolutionary process. Therefore, a meaningful investigation of the molecular changes underlying gene evolution can be provided by comparison of noncoding regulatory regions from closely related species. Previous studies have characterized the C.elegans ovo-related gene lin-48 as a downstream gene of the Pax factor EGL-38, and showed lin-48 functions with egl-38 in the development of hindgut (1). In C. elegans, lin-48 encodes a C2H2 zinc finger protein. It is expressed in head sensory neuron support cells and excretory duct cell as well as hindgut cells. In preliminary studies, we showed that in C. briggsae, the C. elegans lin-48 (Ce-lin-48) promoter was able to drive gfp (green fluorescent protein) expression in the same hindgut cells and neuron support cells as it does in C. elegans. However, expression in the excretory duct cell was essentially eliminated. This result indicated that there is a difference between C. elegans and C. briggsae in either the function or regulation of lin-48. To investigate the molecular nature of this difference, we have isolated the C. briggsae lin-48 gene (Cb-lin-48) from a fosmid clone. A C. briggsae 6.2kb Sal I subclone containing the lin-48 gene could rescue the hindgut and male spicule defects in C. elegans lin-48(sa469) mutants. To test the fuction of lin-48 in C. briggsae, we used RNAi. RNAi treated animals from both species showed low but reproducible levels of lethality, which is similar to C. elegans lin-48 chromosomal mutations (2). We constructed gfp reporter transgenes to investigate the expression pattern of Cb-lin-48. Cb-lin-48::gfp is expressed only in hindgut cells and neuronal support cells in C. briggsae animals, as was observed for the C. elegans gene. This result suggests the expression pattern of lin-48 is different between C. elegans and C. briggsae. Cb-lin-48::gfp in C. elegans animal is likewise expressed only in hindgut cells and neuronal support cells. Thus, at least part of the difference between lin-48 in C. elegans and C. briggsae results from differences in the regulatory sequences. To narrow down the sequences that are critical for the regulation differences between the two species, we did a series of swapping tests. The basic idea behind this is that if any sequences within the regulatory region are important for the regulation differences, the swapping of C.elegans sequences into C.briggsae lin-48 should recover the expression in the excretory duct cell. One swapping clone made up of C.briggsae distal region and C.elegans proximal region showed high excretory duct cell expression. To further narrow down the proximal region, we constructed another swapping clone including 2.4kb C. briggsae distal and about 500bp proximal C. elegans sequence. This clone is able to drive expression of gfp in the excretory duct cell, indicating that this 500bp piece of C. elegans sequence is sufficient for lin-48 transcriptional regulation in this cell. Currently, we are performing a deletion analysis of the C. elegans sequences in this clone. With deletion clones, it is our hope that we will be able to pinpoint the sequence changes that are responsive for the evolutionary changes in gene transcriptional regulation. 1. Johnson, A. D., Fitzsimmons, D., Hagman, J., and Chamberlin, H. M. (2001). Development128: 2857-2865.
2. Chamberlin, H. M., Brown, K. B., Sternberg, P. W., and Thomas, J. H. (1999) Genetics 153: 731-742
We have previously described the isolation, after EMS mutagenesis, of a series of mutants that have lost the ability to avoid the water soluble repellent quinine hydrocloride (qui mutants=QUInine non-avoiders). Five mutants presented an unaltered response to light touch and a wild-type structure of the sensory cilia when stained with the fluorescent dye DiO. One of them, qui-1 (gb 404) showed very low avoidance of quinine and SDS although its avoidance responses to high osmotic strength and Cu ++ appear unchanged. We cloned qui-1 using the SNPs mapping strategy (Wicks et al. 2001). Initially, we positioned qui-1 in a small interval on the LG IV of about 120 kb, between the two SNPs Y45F10A and C08F11A. Then, we rescued the quinine avoidance mutant phenotype by injecting long wild-type PCR products in the qui-1 (gb404) strain. The smallest rescuing fragment was 11 kb and contained a single gene Y45F10B.10, predicted by the C.elegans genome-sequencing consortium. We then sequenced the complete Y45F10B.10 gene in the qui-1 (gb404) strain and found a CAA to TAA transition that generates a stop codon in the first exon. The locus can in principle produce a truncated protein of only 76 aa which presumably has no function. The results obtained in the rescue experiments and those of the sequence of the qui-1 (gb404) indicate that qui-1 corresponds to Y45F10B.10. BLAST search results revealed that QUI-1 is a novel protein of unknown function that contains four WD 40 domains described as functional modules for protein-protein interaction. However the cellular function of QUI-1 cannot, at present, be inferred from its amino acid sequence. Using different GFP fusion constructs we discovered that qui-1 is expressed in a subset of the sensory neurons of the amphid including ASH, ADL that have been previously identified as water soluble avoidance neurons. Using GFP constructs with the entire gene we found that the localization of the protein is not confined to the sensory cilia instead the GFP is diffuse in the cell body, the dendrite, sensory cilia and axon. References: Wicks SR, Yeh RT, Gish WR, Waterston RH, Plasterk RHA. (2001). Rapid gene mapping in Caenorhabditis elegans using a high density polymorphism map. Nature Genetics 28: 160-164.
2 remy@biotec.jouy.inra.fr
The AFD neurone and its postsynaptic interneurons AIY and AIZ have been identified as responsible for thermotaxis in C. elegans. It has been shown by the group of Ikue Mori that nematodes are able to learn and memorise temperature (A. Mohri et al., JWM 2000), as they display preference for and move along isotherms corresponding to their breeding temperature. This experience-dependent behavior called isothermal tracking (IT) suggest that sensory inputs present in the environment during early development can influence adult sensory properties. Genetic analysis involved several molecules in this learning process, including recently the neurone specific calcium-sensor 1 (NCS-1) expressed in the AIY interneurone (Gomez et al., Neuron 2001 Apr; 30(1): 241-8). We already suggested that olfactory imprinting (or odor memory) could exist in nematodes (Remy J.J., EWM 2000). More experiments confirmed now that adults worms can be sensitized to odorant molecules that are present during a short period of their early development. For N2 as well as for a number of other of C. elegans strains, we were able to define a critical period during which worms can learn and memorize short odor inputs. Chemotactic assays performed on adults showed that early exposure to an attractive molecule such as beta-citronellol or benzaldehyde, does sensitize worms to these attractants, but sensitization is restricted to the breeding concentration. Moreover, as for temperature memory, this behaviour is dependent on the presence of food and starved worms showed no odor learning ability. Thus olfactory imprinting and temperature memory seem to share at least three common features : short inputs during early development are efficient, memory of the "intensity" of sensory stimulation (heat level or odor concentration), and food-dependence. It is known that the thermosensory AFD and the chemosensory neurones responsible for attraction to volatil attractants, AWA and AWC, cosynapse the same interneurone AIY, suggesting the possibility of common integration of the two sensations. If this is true, then all mutations affecting temperature memory would also affect olfactive memory. We found for instance that the two alleles ot-22 and ks-5 of the thermotaxis mutant ttx-3, a LIM homeobox exclusively expressed in AIY (Hobert O et al., Neuron 1997, 19:345-357), although not affected in their chemotaxis behaviour, are affected in olfactory learning. These observations reinforce and provide new experimental support to the idea of cointegration of thermo and chemosensory inputs in C. elegans (Pierce-Shimomura, IWM 2001).
The C. elegans gastronome: differential palates for Mycobacterium smegmatis and Mycobacterium tuberculosis
Ming Chih Tsai, 1 Jonathan Lipton, 1 Scott Emmons, John Chan Departments Medicine, Microbiology & Immunology, 1 Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461 It has been observed that certain strains of Pseudomonas aeroginosa, when served as the only food source, are lethal to the nematode worm Caenorhabiditis elegans. This observation has led to the use of C. elegans for the expeditious screening of virulence factors of P. aeroginosa, based on lethality. Importantly, this screen has allowed identification of P. aeuroginosa factors that proved essential for virulence in plant and mice. We have initiated studies to test the suitability of the use of C. elegans to screen for virulence factors in Mycobacterium tuberculosis. The relatively avirulent, rapid-growing M. smegmatis (MC 2 155) was initially used to examine the behavior of the nematode when offered mycobacteria as the sole food source. Preliminary studies revealed that C. elegans readily consumes M. smegmatis. Some worms, however, migrated away from the M. smagmatis lawn in the initial period following transfer to the center of the lawn, only to return later. This temporary avoidance was not observed on the Escherichia coli (OP50) lawn. Interestingly, though not quantified, enhanced sexual activities were observed in worms fed M. smegmatis as the sole diet, as assessed by the increased tendency of pairing of males and hermaphrodites. This apparent enhancement in sexual activities could be the result of aggregative behavior induced by mycobacterial noxious stimuli. The aversion displayed by C. elegans to M. smegmatis was confirmed in another set of experiments. In these studies, eggs derived from bleach-treated gravid worms, deposited on a bacteria-free region of an agar plate seeded with the organism of interest (E. coli, M. smegmatis, or M. tuberculosis), were allowed to hatch and the migration of young larvae to the bacterial lawn monitored. While virtually all hatched larvae migrated to the E. coli lawn within 24 h, those seeded next to the virulent M. tuberculosis lawn avoided the tubercle bacillus completely. Trafficking of the larvae to the relatively avirulent M. smegmatis was apparent, although a significant number of worms still failed to reach the MC 2 155 lawn at the end of 48h. Young larvae obtained by the method described above were also followed to evaluate the effects of different diets on overall C. elegans development and reproduction. On M. smegmatis plates, the ability of young larvae to develop and reproduce appeared to be comparable to those fed E. coli, although the number of eggs produced was not enumerated. In addition, as described above, C. elegans readily feeds on MC 2 155. By contrast, on M. tuberculosis plates, development was markedly stunted: the larvae remained small and moved sluggishly. This specific developmental pattern of M. tuberculosis-fed C. elegans is most likely due to the worms aversion to the tubercle bacillus as a diet, and hence starvation. In summary, the degree of shunning of C. elegans from the relatively avirulent M. smegmatis and the virulent M. tuberculosis differs significantly, as assessed by migration toward the food source, consumption of the specific mycobacterial species, and development. It is also noteworthy that C. elegans mating activities are enhanced when M. smegmatis is offered as the sole food source. Thus, although the C. elegans virulence screen may not be an expeditious or a suitable system for the study of M. tuberculosis pathogenesis, the species-specific effects of mycobacteria on some behaviors of C. elegans as complex as sexual activities and food consumption, and on the developmental process may deserve further investigation.
Shortest Longest ShortestLongest mean mean cycle cycle (sec) (sec) (sec) (sec) N2 20C 49.4 46.2 53.5 44 58 N2 25C 38.2 35.9 42.2 34 44 clk-1 (qm30) 20C 77.5 70.8 86.5 66 92 lin-42 (n1089) 20C 52.8 46.7 58.4 43 65 lin-42 (n1089) 25C 50.5 36.0 62.5 24 91 lin-42 (mg152) 20C 53.3 43.1 71.4 22 118 Strain TemperatureStandardCoefficient Mean ofLowest Highest (30 L4 each) (C) deviation of variationCVs CV CV (%) (%) (%) (%) N2 N2 clk-1 (qm30) lin-42 (n1089) lin-42 (n1089) lin-42 (mg152) 20C 25C 20C 20C 25C 20C 2.2 1.9 5.3 3.6 6.7 7.6 4.5 5.0 6.8 6.8 13.3 14.2 3.5 3.7 4.6 4.2 14.1 23.1 1.7 2.8 2.3 2.3 7.2 10.6 5.5 5.6 6.1 7.6 27.8 46.2
Towards a Pristionchus genome map- I P. pacificus BAC Library construction and end sequencing of BAC clones
Ralf J Sommer 1 , Taco Jesse 2 , Stephan C. Schuster 3 , Gnther Raddatz 3 , Heike Keller 3 , Christa Lanz 3
1 Dept
of Evolutionary Biology, Max Planck Institute for Developmental Biology, Tuebingen, Germany 2 KeyGene NV , Wageningen, The Netherlands 3 Genome Centre, Max Planck Institute for Developmental Biology, Tuebingen, Germany In order to facilitate the easier cloning of genes in the satellite model system Pristionchus pacificus, we have initiated a genomic approach by constructing genetic and physical maps of Pristionchus pacificus. An essential prerequisite for a good genomics approach is the availability of a good genomic library with large insert sizes. We constructed a BAC library of Pristionchus pacificus. The library contained 13, 440 clones in total and was a result of 4 different ligations. The average insert size of the clones is 128 kb. Insert sizes ranged between 100-180kb. We estimate the genome coverage of the library to be approximately 8 times. End sequencing of the BAC clones was done at the Genome Centre of the MPI Tbingen. Uptil now 6000 BAC clones have been end sequenced. On an average approximately 600 bp were read from each BAC end. The sequence was checked for quality using the PHRED/PHREP protocol. The total length of the BAC ends sequenced was approximately 10 Mb. The GC content of the sequence was 47%. From the 11271 ends sequenced, 8232 BAC ends gave no hits and 1129 gave hits to the non-redundant NCBI database.
Towards a Pristionchus genome map II Microevolutionary analysis of the nematode genus Pristionchus
Jagan Srinivasan, Andr Pires da Silva, Arturo Gutierrez, Min Zheng, Hanh Witte, Isabel Kipping, Ralf J. Sommer Dept of Evolutionary Biology, Max Planck Institute for Developmental Biology, Tuebingen, Germany We describe the molecular characterization of laboratory strains of the nematode genus Pristionchus, which lays a foundation for microevolutionary analyses of vulva development. We isolated 13 laboratory strains of the Pristionchus genus that are derived from natural isolates from around the world. Mating experiments and ITS sequence analysis indicated that these 13 strains represent four different species; the gonochoristic species P. lheritieri and three hermaphroditic species, P. pacificus, P. maupasi and an as yet undescribed species Pristionchus sp., respectively. P. pacificus is represented by five different strains isolated from California, Washington, Hawaii, Ontario and Poland. Our working wild type strain is the California strain. Since polymorphisms are becoming an important tool in modern day genomic analysis, which facilitate cloning of mutations, we decided to search for polymorphisms in the various Pristionchus pacificus strains. We performed amplified restriction fragment length polymorphism (AFLP) analyses of the different P. pacificus strains and found that the American strains are highly polymorphic (Srinivasan et al., 2001). We observed the largest genetic variation between the strain from California on the one hand and Washington and Hawaii on the other hand. In contrast, the developmentally distinct strain from Poland is identical to the Californian strain. Hence, we chose the Washington strain as our polymorphic strain for future experiments. These results provide us a framework for further studies on microevolution in developmental processes. Reference: Srinivasan, J., Pires-daSilva, A.; Gutierrez, A.; Jungblut, B.; Zheng, M.; Witte, H.; Schlak., I. & Ralf J. Sommer, Evolution & Development (2001) 3: 229-240.
Towards a Pristionchus genome map III SNP searches and analyses in Pristionchus pacificus
Jagan Srinivasan 1 , Waltraud Sinz 1 , Alexandra Brand 1 , Hanh Witte 1 , Gnther Raddatz 2 , N Ram Kumar 2 , Stephan C. Schuster 2 , Ralf J. Sommer 1
1 Dept
of Evolutionary Biology, Max Planck Institute for Developmental Biology, Tuebingen, Germany 2 Genome Centre, Max Planck Institute for Developmental Biology, Tuebingen, Germany The availability of polymorphic strains of P. pacificus and a genomic BAC library of Pristionchus pacificus with large insert sizes encouraged us to use a BAC end based strategy to achieve our goal of constructing genetic and physical maps of the satellite model system Pristionchus pacificus. We looked for polymorphisms in the sequenced BAC ends using the SSCP ( single stranded conformation polymorphism) technique. The BAC end sequences were obtained from our Genome Centre at the institute and primers were designed to amplify 180-250 bp amplicons within these BAC end sequences. We used an inhouse program Prime Array 3.0 to design the primers. These primers were used to amplify both California and Washington DNAs and the resulting PCR products were run on an agarose gel to check for the presence or absence of bands. Later they were run on an SSCP gel to check for mobility differences. We found a total 131 SNPs . BAC ends that showed mobility differerences were then rechecked by sequencing the Washington PCR product and compared to the already available California sequence. Sequence comparison was done on a commercially available program Sequencher3.0. We found that the percentage of insertions and deletions in Pristionchus pacificus was more than in C.elegans (data from Wicks et al) However the single base pair substitutions were lesser than in C.elegans. This result indicates that insertion and deletion events are more common in Pristionchus pacificus than single base pair substitution events.
Under these assumptions, there would be no net transport of bacteria if the pharynx contracted simultaneously along its entire length and the relaxation were a simple reverse of the contraction. This is actually a very general result: in a system as small as the nematode pharynx, inertial forces can be ignored, and fluid motions are linearly related to the forces that generate them. Such a system can generate no net change if its motions are described by a single degree of freedom. In fact, analysis of videotapes shows that pharyngeal motions are not synchronized along the entire length of the pharynx: the anterior isthmus begins its contraction and relaxation slightly after the corresponding motions of the corpus. When this slight delay is included in the simulation, bacteria assumed to move at the mean fluid velocity are trapped and transported, but very inefficiently. The volume of the anterior isthmus is much less than that of the corpus, so that its influence is small.
The last assumption that went into the simulation is less obvious, but is plausible given the triradiate shape of the pharyngeal lumen (figure above): 3. Bacteria are pushed to the center of the pharyngeal lumen when it closes (figure below).
Fluid moves faster than the mean velocity at the center of a tube, because motion near the walls is slowed by friction. The ratio of center to mean flow velocities can be determined by solving the Poisson equation. Solving Poissons equation for the pharyngeal lumen predicts that the center flow velocity should range from 2.2 to 3.2 times mean velocity, depending on the extent to which the lumen is open. Thus, the third assumption predicts that bacteria will at move 2-3x mean velocity. This increased velocity, it turns out, greatly magnifies the effect of the delayed isthmus motions. The simulated pharynx transports bacteria posteriorly with an efficiency that looks like the real thing. The simulation, along with a more detailed explanation, is available as a Java applet at http://eatworms.swmed.edu/~leon/pharynx_sim/. These results suggest that simple hydrodynamics are indeed sufficient to explain the trapping and transport of bacteria within the pharynx. The most direct way to test whether this mechanism is correct would be high-speed videotapes of the motions of particles in the pharyngeal lumen, which, although technically difficult, is probably possible. Until that can be done, there are other predictions that are simpler to test. First, the model predicts that the changes in anterior isthmus motions should have large effects on the transport of bacteria within the corpus. Second, it predicts that the relative timing of the motions of the corpus and anterior isthmus should be critical. (We have had evidence for this for many years: M3, which control the timing of relaxation, is important for efficient transport of bacteria.) Third, it predicts that asymmetric motions of the pharyngeal muscles, which would tend to drive the bacteria off-center, should decrease the efficiency of bacterial transport.
chemicals by the worms array of cytochrome P450s, and elimination through the intestine by the mechanisms we describe here, could also serve as chemical defenses.
The MAP kinase phosphatase LIP-1 is required for the meiotic cell-cycle arrest in developing oocytes
Alex Hajnal, Erika Frhli Hoier, Thomas Berset Institute of Zoology, University of Zrich, Winterthurerstr. 190, CH-8057 Zrich, Switzerland The RAS/MAP kinase signaling pathway plays an essential role at two steps during hermaphrodite germline development. First, RAS/MAP kinase (MPK-1) signaling is required for the progression of germ cells through the pachytene stage of meiotic prophase I (Lee et al. 2001, IWM abstract 991). After pachytene exit, MPK-1 is rapidly inactivated and the developing oocytes arrest in the diakinesis stage of meiotic prophase I. As the oocytes approach the spermatheca, the secreted sperm signal MSP causes the re-activation of MPK-1, which induces oocyte maturation and allows the meiotic cell cycle to progress (Miller et al. 2001). We have previously shown that the dual specificity phosphatase LIP-1 acts as a negative regulator of MPK-1 during vulval induction (Berset et al. 2001). Here, we report an important role for LIP-1 in regulating MPK-1 activity during germline development. A lip-1 loss-of function-mutation (zh15) suppresses the pachytene arrest caused by reduction-of-function mutations in mpk-1 (oz140 or ga111). Consistent with this genetic interaction, we detected anti-LIP-1 antibody staining in pachytene stage germ cells but no staining at other stages of germline development. Furthermore, the proximal gonads of lip-1(zh15)mutants contain more but smaller oocytes than wild-type gonads (12.34.1 oocytes in lip-1(zh15) as opposed to 7.91.6 in wild-type gonads). The rate of oocyte maturation, on the other hand, is unchanged. Thus, germ cells in lip-1(zh15) mutants exit the pachytene stage at an increased rate, resulting in an overall acceleration of oocyte development. Moreover, by staining gonads with an antibody specific for the diphosphorylated, activated form of MAP kinase (anti-DP-ERK) we found that MPK-1 fails to be inactivated after germ cells exit the pachytene stage in lip-1(zh15)mutants. The increased levels of activated MPK-1 persist throughout the diakinesis stage until fertilization occurs. Interestingly, in 9% of lip-1(zh15) single mutants a few (< 5) unfertilized oocytes per gonad arm display an endomitotic (Emo) phenotype, they start multiple mitotic cell cycles without undergoing cytokinesis. This Emo phenotype is more penetrant (>80%) and stronger (usually >20 mitotic nuclei per gonad arm) in feminized (fem-2 or fog-1) lip-1(zh15) mutants that produce no sperm. Thus, unfertilized oocytes in lip-1(zh15) mutants often fail to arrest the meiotic cell-cycle. Taken together, our results indicate that LIP-1 is responsible for the inactivation of MPK-1 after germ cells exit the pachytene stage. The inactivation of MPK-1 by LIP-1 is critical to allow the developing oocytes to arrest the meiotic cell cycle in the diakinesis stage until oocyte maturation is induced.
(Many thanks to Stuart Kim, Dave Eisenmann, Min Han and the CGC for strains.) [1] Szewczyk and Jacobson, WBG 16(3): 29 (2000) [2] Szewczyk & Jacobson, 2001 International Worm Meeting abstract 554 [3] Lackner and Kim, Genetics 150: 103-117 (1998) [4] Schutzmann, Borland, Newman, Kokel & Stern, Mol. Cell. Biol. (in press) [5] Hara and Han, PNAS 92: 3333-3337 (1995)
Isolation and characterization of suppressors of the ryanodine receptor gene unc-68 mutants
Ryota Adachi, Hiroaki Kagawa Graduate school of Natural Science and Technology, Okayama University, Okayama 700-8530 JAPAN unc-68 encodes the Caenorhabditis elegans ryanodine receptor consisting 5,071 amino acid residues. Most of deletion mutants move slower than the wild-type N2. Only one missense unc-68(kh30) mutant is isolated as a ketamine-response abnormal (Kra) showing convulsions with intermittent paralysis in 30 mM ketamine. The unc-68(kh30) worm produced a full-sized protein having an amino acid substitution at Ser1,444 to Asn which is a putative phosphorylation site of a protein kinase C (Sakube et al., J. Mol. Biol., 267, 849-864, 1997). To investigate molecules interacting with the ryanodine receptor, we isolated revertants from unc-68(e540) and unc-68(kh30) animals. Seven revertants were isolated from unc-68(e540), a nonsense mutant having splicing defect, by choosing faster moving animals. Although the motility of these animals was recovered, the brood size was not. Wild-type N2 bred 330 +/- 49 eggs and 99 % was hatched while unc-68(e540) bred 102 +/- 12 eggs and 87.6 % was hatched. The brood size of revertants was similar to that of unc-68(e540) and hatching rate was about 70 % of the wild-type. We also determined the egg laying rate and defecation cycle. Egg laying rate of the wild-type and unc-68(e540) animals were 7.3 and 1.4 per hour respectively. Egg laying rate in each revertant was two to three times higher than that of unc-68(e540). Although we have not analyzed more details about these unc-68(e540) revertants, these results suggest that in C. elegans, only one ryanodine receptor has different function in body-wall and vulval muscles. On the other hand, five revertants were isolated from unc-68(kh30) by choosing a paralyzed phenotype in 30 mM ketamine like as the wild-type. We tested responses of revertants against some drugs. From the sensitivities to 50 mM caffeine which induces ryanodine receptors to the open-state, five revertants assigned to three groups; the first had high sensitivity, the second had lower sensitivity than the unc-68(kh30), and the last had medium sensitivity between wild-type and unc-68(kh30). We also observed sensitivity to ryanodine; open the channel of ryanodine receptors, levamisole; agonist of acetylcholine receptor, and ouabain; N + , K + -ATPase blocker. We assume that the revertants showing lower sensitivity to drugs have abnormal Ca 2+ control, and one of them showing slow movement and high sensitivity to drugs may be very low Ca 2+ concentration in body-wall muscle. The suppressor gene of the latter was mapped to X chromosome. Using three-factor analysis and cosmid rescuing, this gene was mapped to the region of 0.5 map unit neighbor of lin-15.
Fig. 1: Comparison of the predicted CEH-13 amino acid sequences from the three Caenorhabditis species. The 100% conserved homeodomain is underlined; amino acids that are identical in all three species are in bold.
1) Brunschwig et al. (1999) Development 126:1537-1546 2) Wittmann et al. (1997) Development 124:4193-41200 3) Streit et al. submitted 4) Reto Kohler (1999) Ph.D. thesis, University of Fribourg.
Pujol N. 1 , Zimmer C. 2 , Burglin T. 3 , Cremer H. 2 pujol@ciml.univ-mrs.fr Marseille, France 3 Karolinska Institutet, Huddinge, Sweden
2 IBDM, 1 CIML,
The Cut homeobox gene in Drosophila is required for the specification of neuronal identity in the external sensory organs of the peripheral nervous system. It is also involved in dorsoventral patterning of the wing margin and the formation of Malpighian tubule, tracheal system and some structure in the central nervous system. Two murine homologues of Cut, named Cux-1 and Cux-2 have been cloned. While Cux-1 is expressed in most tissue during development, Cux-2 expression is restricted to the nervous system. Interestingly, ectopic expression of Cux-1 in the fly was shown to rescue the wing scalloping phenotype in a cut mutant. This suggests at least partial conservation of Cut function between invertebrates and mammals (review 1). The sequencing of the C. elegans genome has recently revealed the presence of a Cut-like gene, Y54F10AM.4a, which we call ceh-44. Several Y. Kohara cDNAs confirm the existence of a ceh-44 transcript. It has the same structure as other members of the Cut gene family, consisting of three cut repeats upstream of a cut class homeodomain. Phylogenetic analysis shows that the three cut repeats of CEH-44 are the most divergent of the Cux family, but still cluster with the Cut family. The three cut repeats are 45% (69%), 62% (79%) and 35% (52%) identical (similar) to Drosophila Cut, respectively. The homeodomain of CEH-44 is more divergent and does not cluster significantly with any family, being only 39% identical with mouse Cux-2. The gene is predicted to be the second in an operon downstream of Y54F10AM.5, which has sequence similarity with a NADH-ubiquinone oxidoreductase. Several Y. Kohara cDNAs reveal a spliced form containing the 5 end of the ceh-44 gene, without the cut repeats and the homeodomain (the predicted Y54F10AM.4b form), linked to the predicted adjacent 3 gene Y54F10AM.3. This transcript encodes a protein similar to the vertebrate CASP proteins, the N-terminal part being shared with the vertebrate Cux proteins and CEH-44 (but not Drosophila Cut, which seems to have lost CASP). In the mouse, this protein also arises from alternative splicing of the Cux-1 transcript (2). However, the CASP protein is an evolutionarily old protein, also found in plants and fungi, where it is not associated with an homeobox gene. Thus CASP and an ancestral Cut gene must have merged in early animal evolution. In situ experiments in Y. Koharas database with yk394a11 (3), one of the cDNA corresponding to ceh-44, reveal an early embryonic expression, mostly anterior in the coma stage, as well as in the nerve ring in the larvae and in the gonad in the adult. Our future aim will be to test if ceh-44 in C. elegans functions as a determinant of cell-type specification comparable to Cut in the fly. We will study in more details the ceh-44 expression pattern with GFP fusions and plan to perform loss-of-function and gain-of-function studies. 1. Nepveu A. Gene 2001 2. Lievens P. et al. Gene 1997. 3. http://nematode.lab.nig.ac.jp/cgi-bin/db/ShowGeneInfo.sh?celk=CELK06251
The purpose of this study was to investigate the effect of different concentrations of ascorbic acid in water solutions on nematode life span. In this experiment ascorbic acid was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with ascorbic acid in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21 0 C and in the darkness. The obtained results are presented in the following table. Concentration of ascorbic acid n Longevity (days) MeanS.E.Maximal 1213,71,6 28 12 toxic 1215,41,3 23 1217,91,6 22 1222,00,7 29 1218,61,3 30 1219,31,3 1216,31,1 30 27
Conclusion: If ascorbic acid solution was applied to C. elegans, it was able to increase their mean (by 61,0%, p<0,001) as well as maximal longevity in comparison with control in dilution of 1:10 4 . Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
The purpose of this study was to investigate the effect of different concentrations of vitamin B 12 in water solutions on nematode life span. In this experiment vitamin B 12 was used in following dilutions: 1:10 4 , 1:10 5 , 1:10 6 , 1:10 7 , 1:10 8 and 1:10 9 . Three adult animals (3 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coliand without vitamin B 12 ) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (with vitamin B 12 in any concentration) every day (one worm in one well) beginning from third day. This investigation was carried out in temperature +21 0 C and in the darkness. The obtained results are presented in the following table. Concentration of vitamin B 12 n Longevity (days) MeanS.E.Maximal 1217,81,9 30 12 18,82,3 32 1219,71,6 37 1215,31,1 26 1213,91,0 28 1217,41,1 30 1216,61,2 30
Conclusion: If vitamin B 12 solution was applied to C. elegans, it was not able to increase significantly their mean longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
An attempt to slow aging in C. elegans. No positive effect of ascorbic acid in postreproductive period
Vladimir V. Bakaev 1,2
1 Box 2 E-mail:
The purpose of this study was to investigate the effect of different concentrations of ascorbic acid in water solutions on the nematode life span in postreproductive period. In this experiment ascorbic acid was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without ascorbic acid in medium) every day (one worm in one well) beginning from third day. Then, beginning from 10th day, these worms were transferred every day in next wells containing medium with ascorbic acid in any concentration. This investigation was carried out in temperature +21 C and in the darkness. The obtained results are presented in the following table. Concentration of ascorbic acid n Longevity (days) MeanS.E.Maximal 12 19,91,0 24 12 toxic 12 9,10,1 10 12 14,50,7 22 12 14,00,5 22 12 16,10,9 26 12 18,21,1 12 16,30,7 31 22
Conclusion: If ascorbic acid solution was applied to C. elegans in postreproductive period, it was not able to increase their mean longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans(Bristol, N2) and E. coli OP50.
An attempt to slow aging in C. elegans. 23. No positive effect of ascorbic acid in reproductive period
Vladimir V. Bakaev 1,2
1 Box 2 E-mail:
The purpose of this study was to investigate the effect of different concentrations of ascorbic acid in water solutions on the nematode life span in reproductive period. In this experiment ascorbic acid was used in following dilutions: 1:10 1 , 1:10 2 , 1:10 3 , 1:10 4 , 1:10 5 , 1:10 6 and 1:10 7 . Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,5 ml of liquid medium (with E. coli and without ascorbic acid) during 4 hours, then they were discarded and newborn larvae were transferred in next wells (without ascorbic acid in medium) every day (one worm in one well) beginning from third day. Then, from 3th to 10th day, these worms were transferred every day in next wells containing medium with ascorbic acid in any concentration. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Concentration of ascorbic acid n Longevity (days) MeanS.E.Maximal Control 12 12,81,1 18 1 12 toxic 1:10 12 13,01,3 21 1:10 2 3 12 13,70,7 21 1:10 4 12 14,00,6 23 1:10 5 12 14,80,7 21 1:10 6 12 10,80,5 17 1:10 7 12 13,90,8 23 1:10 Conclusion: If ascorbic acid solution was applied to C. elegans in reproductive period, it was not able to increase their mean longevity in comparison with control. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.
We have been studying a mutation called gas-1 that was isolated in a screen for hypersensitivity to the volatile anesthetic isoflurane. This animal is hypersensitive to all the volatile anesthetics in which it has been tested, has a shortened lifespan, produces only 50 or so eggs at room temperature, and is basically a temperature sensitive lethal. gas-1 encodes a subunit of the first protein complex of the electron transport chain. We>have done a great deal of metabolic measurements to characterize the defect in this animal. The ability of mitochondria from gas-1 to metabolize complex I specific substrates like malate or glutamate is about 1/4 that of N2 mitochondria. Their ability to use succinate, a substrate specific for complex II, is greater than N2. gas-1 animals possess the same ratio of ATP to ADP + AMP as N2 when in air, but this ratio drops when they are immobilized in a volatile anesthetic. We are now mapping two suppressors of the anesthetic sensitive phenotype. These two mutations are dominant, with no clear phenotype of their own for mapping. The suppressors do extend the lifespan of gas-1, although they do not restore it to normal. This is also true for the ability of their mitochondria to metabolize malate or glutamate. We have now produced an antibody to GAS-1 that is designed to distinguish it from another protein that may be expressed from a virtually identical cDNA that is contained on the cosmid T26A5.3. Although we know this sequence is expressed, we have not seen any signal via a GFP promoter, whereas a gas-1 construct apppears widely expressed. T26A5.3 does not rescue gas-1 unless the sequence is placed under the gas-1 promoter. We are also trying to identify the different complexes of the electron transport chain via a native gel in order to see the stoichiometry of the subunits of complex I in a gas-1 animal. Although we can estimate weights of each complex based on the predictions of genomic sequences of each subunit of each complex, commercial antibodies so far have not helped us distinguish Complex I from the other four major bands on gels. Another major effort on this project is the identification of the kinds of damage generated by defective mitochondria in gas-1 animals. Westerns that are probed with an antibody to products of lipid peroxidation and also gels using a kit that measures oxidative damage to proteins show that gas-1 mitochondria undergo more oxidative damage than N2. We are planning to identify the major proteins damaged in gas-1 mitochondria. Since we are anesthesiologists, we have also been interested in patients with mitochondiral myopathies. There have long been anecdotal reports of children with mitochondrial defects that are hypersensitive to anesthetics. We have some observations on children that have come to our operating room for either diagnosis or treatment of a mitochondrial disease, that would suggest that different defects may lead to different sensitivities to a volatile anesthetic. Only those few children we observed with a complex I defect were hypersensitive (4 children out of 16 patients). Children with defects in Complex II were not hypersensitive. We know that mev-1, a defect in Complex II in C. elegans, shares many phenotypes with gas-1, but is not hypersensitve to volatile anesthetics. It cannot use succinate as a substrate for oxygen consumption. Isolation of mitochondria, measurements of ATP, extraction of mitochondrial proteins, and attempts to age-match very large numbers of mutant worms have also absolutely demonstrated to us the reasons biochemistry is not much fun in C. elegans. Once in a while we do a nice mating just to satisfy a longing for the good old days.
Author index
Theresa Stiernagle, Bob Herman University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Avenue, St. Paul, MN 55108-1095 Adachi, R Ailion, M Avery, L Bakaev, V Barstead, R Bazzicalupo, P Berset, T Blaxter, M Brand, A Burglin, T Chamberlin, H Chan, J Clegg, E Cremer, H Culotti, J Edgley, E Emmons, S Fonte, G Fos, J French, D Gautron, F Gilchrist, E Gutierrez, A Hajnal, A Hammarlund, M Hare, E Heise, C Herman, B Hilliard, M Hobert, O Hoier, E Hubbard, E Jacobson, L Jesse, T Jones, S Jorgensen, E Kagawa, H Keller, H 58 39 52, 54 64-67 59 43 55 46 50, 51 63 40-42 45 33 63 37 30 45 36 62 33 62 30 49 55 38 60 54 3 43 34 55 32 56 48 30 38 58 48
Killian, D Kippert, F Kipping, I Kumar, R Lanz, C LaPenotiere, H Levy-Strumpf, N Link, Lipton, J Loer, C McKay, S Moerman, D Morgan, P Moulder, G Mullen, G Mller, F Pires da Silva, A Plasterk, R Pujol, N Raddatz, G Rajakumar, V Rand, J Remy, J Schuster, S Sedensky, M Sinz, W Smith, H Sommer, R Srinivasan, J Stiernagle, T Stoyanov, C Streit, A Szewczyk, N Szilagyi, M Taft, A Thomas, J Tsai, M Uttam, A Wang, X Ward, S Witte, H WormBase Consortium Zheng, M
Zimmer, C
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